Zygote (Cambridge, England)最新文献

{"title":"Analysis of trophectoderm markers in domestic cat blastocysts cultured without zona pellucida.","authors":"Daniel Veraguas-Dávila,&nbsp;Darling Saéz-Ruíz,&nbsp;María Consuelo Álvarez,&nbsp;Fernando Saravia,&nbsp;Fidel Ovidio Castro,&nbsp;Lleretny Rodríguez-Alvarez","doi":"10.1017/S096719942200034X","DOIUrl":"https://doi.org/10.1017/S096719942200034X","url":null,"abstract":"<p><p>Domestic cat embryos generated by <i>in vitro</i> fertilization (IVF) and cultured without the zona pellucida have a reduced implantation capacity after embryo transfer at the blastocyst stage. The objective of this study was to evaluate the expression of trophectoderm markers in domestic cat blastocysts cultured without the zona pellucida. Two experimental groups were selected: (1) domestic cat embryos generated by IVF and cultured <i>in vitro</i> normally (zona intact group, ZI); and (2) domestic cat embryos generated by IVF and cultured <i>in vitro</i> without a zona pellucida (zona-free group, ZF). In the ZF group, the zona pellucida of the presumptive zygote was removed and these were cultured using the well of the well (WOW) system. <i>In vitro</i> culture was carried out for 7 days. The cleavage, morula and blastocyst rates were estimated. Finally, the relative expression levels of the trophectoderm markers <i>TEAD4</i>, <i>YAP1</i>, <i>CDX2</i> and <i>EOMES</i>, the cell adhesion marker <i>E-cadherin</i> and the apoptosis marker <i>CASP3</i> were evaluated by RT-qPCR in the blastocysts. The Wilcoxon test was used to evaluate differences (<i>P</i> < 0.05). No differences were observed in the cleavage, morula and blastocyst rates between the ZF and ZI groups. No differences were found in the expression of <i>TEAD4</i>, <i>CDX2</i>, <i>E-cadherin</i> and <i>CASP3</i> between groups. The expression of <i>YAP1</i> and <i>EOMES</i> was higher in ZF blastocysts than in ZI blastocysts. In conclusion, the <i>in vitro</i> culture without the zona pellucida generates an overexpression of <i>YAP1</i> and <i>EOMES</i> in the domestic cat blastocysts. More studies are needed to confirm if this overexpression might affect <i>in vivo</i> development.</p>","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"841-848"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40333870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boar semen cryopreserved with trehalose-containing liposomes: disaccharide determination and rheological behaviour.","authors":"Claudia Denisse Mendoza-Viveros,&nbsp;Oscar Gutiérrez-Pérez,&nbsp;María Josefa Bernad-Bernad,&nbsp;Luis Medina-Torres,&nbsp;Minerva Monroy-Barreto,&nbsp;Miquel Gimeno,&nbsp;María Elena Trujillo-Ortega","doi":"10.1017/S0967199422000442","DOIUrl":"https://doi.org/10.1017/S0967199422000442","url":null,"abstract":"This study aimed to detect intracellular trehalose in boar sperm that were cryopreserved with liposomes and conduct an analysis of its effects on some characteristics of thawed sperm, including rheological properties. First, soybean lecithin cholesterol-based liposomes were produced and characterized in the presence of 300 mM trehalose. Next, semen samples were frozen in two freezing media: a control medium with 300 mM trehalose and an experimental medium supplemented with 300 mM trehalose and 10% liposomes, both of which were thawed and then studied to ascertain their integrity, motility, rheological response, and trehalose quantities by testing two methods of spermatic lysis via high-performance liquid chromatography with an evaporative light-scattering detector (HPLC-ELSD). The results found spherical liposomes measuring 357 nm that were relatively stable in an aqueous medium and had an entrapment efficiency of 73%. An analysis of the cryopreserved ejaculates showed that their viability and motility did not significantly differ between groups (P > 0.05). The viscous response of the samples was influenced by the extracellular medium rather than by the freezing-thawing process, which resulted in a loss of interaction between the cells and cryoprotectants. Finally, intracellular trehalose levels were determined using HPLC-ELSD, with no differences observed (P > 0.05) when comparing both sperm lysis methods. The use of liposomes with trehalose appears to be a promising option for boar semen cryopreservation, with a marked effect on rheological properties. The proposed HPLC-ELSD method was effective for measuring trehalose in cryopreserved cell samples.","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"895-902"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40360212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategy for embryo transfer to improve pregnancy outcomes in advanced maternal age.","authors":"Xue Wang,&nbsp;Yaling Xiao,&nbsp;Zhengyi Sun,&nbsp;Jingran Zhen,&nbsp;Qi Yu","doi":"10.1017/S0967199422000259","DOIUrl":"https://doi.org/10.1017/S0967199422000259","url":null,"abstract":"<p><p>The purpose of this retrospective study was to optimize the transplantation strategy for women of advanced maternal age to achieve live births within the shortest time. Data were collected from patients older than 40 years who underwent assisted reproductive therapy at our centre from 1 January 2009 to 31 December 2019. In total, 1023 cases of fresh cleavage embryo transfer (CET) cycles, 280 cases of frozen-thawed blastocyst transfer (FBT) cycles, and 26 cases of frozen-thawed CET (FCET) cycles were included. The main outcome was the live birth rate (LBR). The secondary outcomes were the clinical pregnancy rate (CPR) and neonatal outcomes. Multivariable logistic regression was performed to adjust for confounding factors. The blastocyst formation rate of patients older than 40 years was 23.5%, the freezing cycle rate was 19.8%, and the fresh-embryo transfer rate was 83.0%. The implantation rate, CPR, and LBR were significantly different among the CET, FCET, and FBT groups. There were no significant differences in multiple pregnancies and abortion rates among the groups, and neonatal outcomes were similar. Multivariate logistic regression analysis showed that, compared with the CET group, LBR did not increase in the FCET group, whereas LBR increased in the FBT group. For patients older than 40 years when having approximately eight embryos after fertilization, blastocyst transfer can be considered after fully discussing the advantages and disadvantages of blastocyst culture. Alternatively, CET can be performed first, followed by FBT if the cleavage embryo transfer is unsuccessful.</p>","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"781-789"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40634285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplementation of porcine <i>in vitro</i> maturation medium with FGF2, LIF, and IGF1 enhances cytoplasmic maturation in prepubertal gilts oocytes and improves embryo quality.","authors":"María Serrano Albal,&nbsp;Giuseppe Silvestri,&nbsp;Lucas G Kiazim,&nbsp;Lucy M Vining,&nbsp;Louisa J Zak,&nbsp;Grant A Walling,&nbsp;Alexandra M Haigh,&nbsp;Simon C Harvey,&nbsp;Katie E Harvey,&nbsp;Darren K Griffin","doi":"10.1017/S0967199422000284","DOIUrl":"https://doi.org/10.1017/S0967199422000284","url":null,"abstract":"<p><p>In porcine <i>in vitro</i> production (IVP) systems, the use of oocytes derived from prepubertal gilts, whilst being commercially attractive, remains challenging due to their poor developmental competence following <i>in vitro</i> maturation (IVM). Follicular fluid contains important growth factors and plays a key role during oocyte maturation; therefore, it is a common supplementation for porcine IVM medium. However, follicular fluid contains many poorly characterized components, is batch variable, and its use raises biosecurity concerns. In an effort to design a defined IVM system, growth factors such as cytokines have been previously tested. These include leukaemia inhibitory factor (LIF), fibroblast growth factor 2 (FGF2), and insulin-like growth factor 1 (IGF1), the combination of which is termed 'FLI'. Here, using abattoir-derived oocytes in a well established porcine IVP system, we compared follicular fluid and FLI supplementation during both IVM and embryo culture to test the hypothesis that FLI can substitute for follicular fluid without compromising oocyte nuclear and cytoplasmic maturation. We demonstrate that in oocytes derived from prepubertal gilts, FLI supplementation enhances oocyte meiotic maturation and has a positive effect on the quality and developmental competence of embryos. Moreover, for the first time, we studied the effects of follicular fluid and FLI combined showing no synergistic effects.</p>","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"801-808"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40336493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic and aberrant patterns of H3K4me3, H3K9me3, and H3K27me3 during early zygotic genome activation in cloned mouse embryos.","authors":"Zhihui Liu,&nbsp;Jing Cui,&nbsp;Weiguo Wang,&nbsp;Mingyang Li,&nbsp;Zhisong Wang,&nbsp;Giorgio Antonio Presicce,&nbsp;Xiuchun Cindy Tian,&nbsp;Liyou An,&nbsp;Fuliang Du","doi":"10.1017/S0967199422000454","DOIUrl":"https://doi.org/10.1017/S0967199422000454","url":null,"abstract":"<p><p>Somatic cell nuclear transfer (NT) is associated with aberrant changes in epigenetic reprogramming that impede the development of embryos, particularly during zygotic genome activation. Here, we characterized epigenetic patterns of H3K4me3, H3K9me3, and H3K27me3 in mouse NT embryos up to the second cell cycle (i.e. four-celled stage) during zygotic genome activation. <i>In vivo</i> fertilized and parthenogenetically activated (PA) embryos served as controls. In fertilized embryos, maternal and paternal pronuclei exhibited asymmetric H3K4me3, H3K9me3, and H3K27me3 modifications, with the paternal pronucleus showing delayed epigenetic modifications. Higher levels of H3K4me3 and H3K9me3 were observed in NT and PA embryos than in fertilized embryos. However, NT embryos exhibited a lower level of H3K27me3 than PA and fertilized embryos from pronuclear stage 3 to the four-celled stage. Our finding that NT embryos exhibited aberrant H3K4me3, H3K9me3, and H3K27me3 modifications in comparison with fertilized embryos during early zygotic genome activation help to unravel the epigenetic mechanisms of methylation changes in early NT reprogramming and provide an insight into the role of histone H3 in the regulation of cell plasticity during natural reproduction and somatic cell NT.</p>","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"903-909"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40360213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survival analysis for estimating lamb survival up to weaning in Harnali sheep.","authors":"Parth Gaur,&nbsp;Z S Malik,&nbsp;Yogesh C Bangar,&nbsp;Ankit Magotra,&nbsp;Ashish Chauhan","doi":"10.1017/S0967199422000272","DOIUrl":"https://doi.org/10.1017/S0967199422000272","url":null,"abstract":"<p><p>The present study was carried out to estimate lamb survival (in days) from birth to weaning under survival analysis using data records from 2057 Harnali lambs born to 134 sires and 623 dams between the period from 2001 to 2020. The weaning age in resourced population was 90 days from birth. The hazard ratio in terms of risk of death up to weaning was determined using Cox proportional hazards model by subjecting some fixed factors such as year of birth, sex of lamb, birth weight (kg), dam's weight at lambing (kg) and dam's age at lambing (years). The overall survivability up to weaning among lambs was 91.59% and Kaplan-Meier estimates of mean survival time up to weaning was 85.77 days. Cox proportional hazard modelling revealed that the hazards of death up to weaning was higher in male lambs [1.66, 95% confidence interval (CI): 1.22-2.26] compared with female lambs [hazard ratio (HR) = 1.00]. It was also observed that the hazards of death (HR = 0.91, 95% CI: 0.88-0.94) had decreasing trends over years. For birth weight (kg), hazard rate was 0.34 (95% CI: 0.25-0.46), which indicated that the risk of pre-weaning mortality was lower as birth weight increases. The weight and age of dams at lambing did not influence the survival time of studied population. The present findings indicated that survival time increased in studied lambs over the years and it could be increased more by giving more emphasis on better litter weight and general health aspects at farm level.</p>","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"797-800"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40574456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of the impact of COVID-19 on male reproduction, and its implications on assisted reproductive technology services.","authors":"Romualdo Sciorio,&nbsp;Luca Tramontano,&nbsp;Serena Bellaminutti,&nbsp;Raffaele Aiello,&nbsp;Adriana Fortunato,&nbsp;Roberto Marci,&nbsp;Ronny Janssens","doi":"10.1017/S0967199421000666","DOIUrl":"https://doi.org/10.1017/S0967199421000666","url":null,"abstract":"<p><p>The announcement in 2019 of a new coronavirus disease that quickly became a major pandemic, is an exceptional challenge to healthcare systems never seen before. Such a public health emergency can largely influence various aspects of people's health as well as reproductive outcome. IVF specialists should be vigilant, monitoring the situation whilst contributing by sharing novel evidence to counsel patients, both pregnant women and would-be mothers. Coronavirus infection might adversely affect pregnant women and their offspring. Consequently, this review paper aims to analyse its potential risks for reproductive health, as well as potential effects of the virus on gamete function and embryo development. In addition, reopening fertility clinics poses several concerns that need immediate addressing, such as the effect of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) on reproductive cells and also the potential risk of cross-contamination and viral transmission. Therefore, this manuscript summarizes what is currently known about the effect of the SARS-CoV-2 infection on medically assisted reproductive treatments and its effect on reproductive health and pregnancy.</p>","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"743-748"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40356515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Hsp90 during <i>in vitro</i> maturation under thermoneutral or heat shock conditions compromises the developmental competence of bovine oocytes.","authors":"Eliza Diniz de Souza,&nbsp;Jessica Fernanda da Silva E Souza,&nbsp;Pedro Manoel de Oliveira Netto,&nbsp;Luciano de Rezende Carvalheira,&nbsp;Ribrio Ivan Tavares Pereira Batista,&nbsp;Carolina Capobiango Romano Quintão,&nbsp;Iuri Drumond Louro,&nbsp;Luiz Sergio Almeida Camargo","doi":"10.1017/S0967199422000387","DOIUrl":"https://doi.org/10.1017/S0967199422000387","url":null,"abstract":"<p><p>Heat shock protein 90 (Hsp90) is critical for cell homeostasis but its role on bovine oocyte maturation is not well known. We investigated the importance of Hsp90 for competence of bovine oocyte using 17-(allylamino)-17-demethoxygeldanamycin (17AAG), an inhibitor of Hsp90, during <i>in vitro</i> maturation (IVM). Three experiments evaluated the effect of 17AAG on developmental competence of oocytes matured <i>in vitro</i> under thermoneutral (38.5ºC) or heat shock (HS; 41.5ºC) temperatures. The first experiment found that the blastocyst rates were lower (<i>P</i> < 0.05) with 2 µM 17AAG compared with the untreated control (0 µM). The abundance of <i>HSF1</i> transcripts was higher in oocytes matured with 2 µM than with 0 and 1 µM 17AAG, whereas the abundance of <i>HSP90AA1</i> and <i>HSPA1A</i> transcripts was lower (<i>P</i> < 0.05) with 1 and 2 µM than with 0 µM. The second experiment found that 2 µM 17AAG for 12 or 24 h during IVM decreased (<i>P</i> < 0.05) the blastocysts rates. In the third experiment, the association of 2 μM 17AAG with HS for 12 h during IVM resulted in lower (<i>P</i> < 0.05) blastocysts rates than 17AAG, HS or untreated control. In conclusion, inhibition of Hsp90 during <i>in vitro</i> maturation compromises further embryo development; the association of Hsp90 inhibition with HS aggravates the deleterious effect of both on oocyte developmental competence.</p>","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"854-862"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40360202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric evaluation of two-pronucleus zygote images using image-processing techniques.","authors":"Niloofar Sayadi,&nbsp;Sara Monji-Azad,&nbsp;Seyed Abolghasem Mirroshandel,&nbsp;Fatemeh Ghasemian","doi":"10.1017/S0967199422000326","DOIUrl":"https://doi.org/10.1017/S0967199422000326","url":null,"abstract":"<p><p>Identifying embryos with a high potential for implementation remains a challenge in <i>in vitro</i> fertilization (IVF) cycles. Despite progress in IVF treatment, only a minority of generated embryos has the ability to implant. Another drawback of this practice is the high frequency of multiple pregnancies. This problem leads to economic and health problems. Therefore, the transfer of a single embryo with high implantation potential is the ideal strategy. Morphometric evaluation of two-pronucleus zygote images is a helpful technique when aiming to transfer a single embryo with a high implantation potential. In this study, an automated zygote morphometric evaluation algorithm, called the zygote morphology evaluation (ZME) algorithm, was created to analyze the zygote and provide morphological measurements. The first and most crucial step of the ZME algorithm is the noise reduction step, which was first applied to zygote images. After that, the proposed algorithm detects different parts of the zygote that are indicators of embryo viability and normality, that is the oolemma, perivitelline space, zona pellucida, and nucleolar precursor bodies (NPBs). In addition, a novel dataset was prepared for this task. This dataset consisted of 703 human zygote images, and called the human zygote morphometric evaluation dataset (HZME-DS). Our experimental results in the HZME-DS showed that the ZME algorithm was able to achieve 79.58% average accuracy in identifying the oolemma region, 79.40% average accuracy in determining the perivitelline space, and 79.72% accuracy in identifying the zona pellucida. To calculate the accuracy of identifying NPBs, the proposed algorithm uses Recall and Precision measures, and their harmonic average (F1 measure) reached values of 81.14% and 79.53%, respectively. These encouraging results for our proposed method, which is an automatic and very fast method, showed that the ZME algorithm could help embryologists to evaluate the best zygotes in real time and the best embryos subsequently.</p>","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"819-829"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40618624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embryo manipulation in neotropical characiform fish: incubation system, anaesthetic, and PGC transplantation in <i>Prochilodus lineatus</i>.","authors":"Gabriella Braga Carvalho,&nbsp;Geovanna Carla Zacheo Coelho,&nbsp;Andreoli Correia Alves,&nbsp;Amanda Pereira Dos Santos Silva,&nbsp;Paulo Sérgio Monzani,&nbsp;José Augusto Senhorini,&nbsp;Norberto Castro Vianna,&nbsp;George Shigueki Yasui","doi":"10.1017/S0967199422000211","DOIUrl":"https://doi.org/10.1017/S0967199422000211","url":null,"abstract":"<p><p>Primordial germ cells transplantation is a unique approach for conservation and reconstitution of endangered fish species. This study aimed to establish techniques to culture dechorionated embryos in different incubation systems and also to determine anaesthetic concentration for fish recipients in the larval stage for subsequent primordial germ cell transplantation. Intact and dechorionated embryos were divided into three incubation systems: (1) a control group with manual replacement of the solution; (2) a closed environment with high oxygen with manual replacement of the solution; and (3) constant solution recirculation. This combination resulted in six treatments. For the evaluation of anaesthetics for larvae, the concentrations evaluated were 19.5 mM, 24.4 mM, 29.3 mM, and 34.2 mM of 2-phenoxyethanol. Anaesthesia concentration and recovery at different stages were evaluated. For transplantation, primordial germ cells of <i>Astyanax altiparanae</i> were transplanted into anaesthetised larvae (1 dph) of <i>Prochilodus lineatus</i>. Better results were obtained in the recirculation system for dechorionated embryos of <i>P. lineatus</i> for hatching (54.18%) and normal morphology (50.06%). The 2-phenoxyethanol anaesthetic with a dose of 29.3 mM resulted in shorter induction times, in addition to the recovery time between 5 and 10 min. By using this anaesthetic concentration at transplantation, GFP-positive cells were seen in two recipients, but the cells did not proliferate. This study established an effective incubation system for the development of the dechorionated embryo and determined an effective anaesthetic concentration for <i>P. lineatus</i> larvae. In addition, micromanipulation and transplantation of primordial germ cells in neotropical species were conducted for the first time.</p>","PeriodicalId":136608,"journal":{"name":"Zygote (Cambridge, England)","volume":" ","pages":"773-780"},"PeriodicalIF":1.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40672770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}