The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.

Zygote (Cambridge, England) Pub Date : 2015-06-01 Epub Date: 2014-03-05 DOI:10.1017/S096719941400001X

Sung-Sik Kang, Sosuke Ofuji, Kei Imai, Weiping Huang, Keisuke Koyama, Yojiro Yanagawa, Yoshiyuki Takahashi, Masashi Nagano

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引用次数: 10

Abstract

The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.

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观察了WOW培养体系对牛小群体胚胎发育的影响及相邻胚胎数量对其发育的影响。

本研究的目的是阐明WOW培养体系对少量胚的培养效果，以及WOW培养皿中相邻胚数对囊胚发育的影响。在常规滴培养中，与对照组(25-26个胚胎/滴)相比，小数量组(5-6个胚胎/滴)的囊胚发育较低。然而，在WOW培养皿中，少量和大量胚胎(分别为5-6个和25个胚胎)与对照组(25-30个胚胎/滴)相比，在卵裂、囊胚率和囊胚平均细胞数方面没有显著差异。此外，WOW培养皿中相邻胚胎的数量对囊胚发育和囊胚细胞数量没有影响。综上所述，无论将胚胎置于WOW培养皿的微孔中，WOW培养皿都能在小群体培养中提供高且稳定的囊胚发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zygote (Cambridge, England)

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