Inflammation and Regeneration最新文献

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Morphology-based noninvasive early prediction of serial-passage potency enhances the selection of clone-derived high-potency cell bank from mesenchymal stem cells. 基于形态学的无创序列传代效能早期预测提高了间充质干细胞克隆衍生高效细胞库的选择。
IF 8.1 3区 医学
Inflammation and Regeneration Pub Date : 2022-10-02 DOI: 10.1186/s41232-022-00214-w
Takashi Suyama, Yuto Takemoto, Hiromi Miyauchi, Yuko Kato, Yumi Matsuzaki, Ryuji Kato
{"title":"Morphology-based noninvasive early prediction of serial-passage potency enhances the selection of clone-derived high-potency cell bank from mesenchymal stem cells.","authors":"Takashi Suyama,&nbsp;Yuto Takemoto,&nbsp;Hiromi Miyauchi,&nbsp;Yuko Kato,&nbsp;Yumi Matsuzaki,&nbsp;Ryuji Kato","doi":"10.1186/s41232-022-00214-w","DOIUrl":"https://doi.org/10.1186/s41232-022-00214-w","url":null,"abstract":"<p><strong>Background: </strong>Rapidly expanding clones (RECs) are one of the single-cell-derived mesenchymal stem cell clones sorted from human bone marrow mononuclear cells (BMMCs), which possess advantageous features. The RECs exhibit long-lasting proliferation potency that allows more than 10 repeated serial passages in vitro, considerably benefiting the manufacturing process of allogenic MSC-based therapeutic products. Although RECs aid the preparation of large-variation clone libraries for a greedy selection of better-quality clones, such a selection is only possible by establishing multiple-candidate cell banks for quality comparisons. Thus, there is a high demand for a novel method that can predict \"low-risk and high-potency clones\" early and in a feasible manner given the excessive cost and effort required to maintain such an establishment.</p><p><strong>Methods: </strong>LNGFR and Thy-1 co-positive cells from BMMCs were single-cell-sorted into 96-well plates, and only fast-growing clones that reached confluency in 2 weeks were picked up and passaged as RECs. Fifteen RECs were prepared as passage 3 (P3) cryostock as the primary cell bank. From this cryostock, RECs were passaged until their proliferation limitation; their serial-passage limitation numbers were labeled as serial-passage potencies. At the P1 stage, phase-contrast microscopic images were obtained over 6-90 h to identify time-course changes of 24 morphological descriptors describing cell population information. Machine learning models were constructed using the morphological descriptors for predicting serial-passage potencies. The time window and field-of-view-number effects were evaluated to identify the most efficient image data usage condition for realizing high-performance serial-passage potency models.</p><p><strong>Results: </strong>Serial-passage test results indicated variations of 7-13-repeated serial-passage potencies within RECs. Such potency values were predicted quantitatively with high performance (RMSE < 1.0) from P1 morphological profiles using a LASSO model. The earliest and minimum effort predictions require 6-30 h with 40 FOVs and 6-90 h with 15 FOVs, respectively.</p><p><strong>Conclusion: </strong>We successfully developed a noninvasive morphology-based machine learning model to enhance the efficiency of establishing cell banks with single-cell-derived RECs for quantitatively predicting the future serial-passage potencies of clones. Conventional methods that can make noninvasive and quantitative predictions without wasting precious cells in the early stage are lacking; the proposed method will provide a more efficient and robust cell bank establishment process for allogenic therapeutic product manufacturing.</p>","PeriodicalId":13588,"journal":{"name":"Inflammation and Regeneration","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2022-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40388208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Identification of molecular subtypes based on inflammatory response in lower-grade glioma. 基于低级别胶质瘤炎症反应的分子亚型鉴定。
IF 8.1 3区 医学
Inflammation and Regeneration Pub Date : 2022-10-01 DOI: 10.1186/s41232-022-00215-9
Wanzun Lin, Jing Gao, Haojiong Zhang, Li Chen, Xianxin Qiu, Qingting Huang, Jiyi Hu, Lin Kong, Jiade J Lu
{"title":"Identification of molecular subtypes based on inflammatory response in lower-grade glioma.","authors":"Wanzun Lin,&nbsp;Jing Gao,&nbsp;Haojiong Zhang,&nbsp;Li Chen,&nbsp;Xianxin Qiu,&nbsp;Qingting Huang,&nbsp;Jiyi Hu,&nbsp;Lin Kong,&nbsp;Jiade J Lu","doi":"10.1186/s41232-022-00215-9","DOIUrl":"https://doi.org/10.1186/s41232-022-00215-9","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory response is an important characteristic affecting prognosis and therapeutic response in lower-grade glioma (LGG). However, the molecular subtypes based on inflammatory response are still under exploitation.</p><p><strong>Methods: </strong>The RNA sequencing, somatic mutation, and corresponding clinical data from 1205 LGG patients were obtained from the TCGA, CGGA, and Rembrandt cohorts. Consensus clustering was performed to identify molecular subtypes associated with inflammation. Prognosis, clinicopathologic features, immune cell infiltration, and somatic mutation profile were compared among these inflammation-associated subtypes.</p><p><strong>Results: </strong>Our results demonstrate that LGG could be categorized into inflammation-, low, -mid, and -high subtypes with distinct clinicopathologic features, prognostic and tumor microenvironment. We established that this categorization was reproducible, as well as predictable. In general, inflammation-high subtype presents a dismal prognosis with the immunosuppressive microenvironment and high frequency of oncogene mutation. Inversely, inflammation-low subtype was associated with the most favorable clinical outcomes with the immunoreactive microenvironment among three subtypes. Moreover, we develop and validate an inflammation-related prognostic model, which shows strong power for prognosis assessment.</p><p><strong>Conclusion: </strong>In conclusion, we established a novel glioma classification based on the inflammation subtype. This classification had significant outcomes for estimating the prognosis, as well as the tumor microenvironment.</p>","PeriodicalId":13588,"journal":{"name":"Inflammation and Regeneration","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40384481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Imatinib inhibits pericyte-fibroblast transition and inflammation and promotes axon regeneration by blocking the PDGF-BB/PDGFRβ pathway in spinal cord injury. 伊马替尼通过阻断脊髓损伤PDGF-BB/PDGFRβ通路抑制周细胞-成纤维细胞转化和炎症,促进轴突再生。
IF 8.1 3区 医学
Inflammation and Regeneration Pub Date : 2022-09-26 DOI: 10.1186/s41232-022-00223-9
Fei Yao, Yang Luo, Yan-Chang Liu, Yi-Hao Chen, Yi-Teng Li, Xu-Yang Hu, Xing-Yu You, Shui-Sheng Yu, Zi-Yu Li, Lei Chen, Da-Sheng Tian, Mei-Ge Zheng, Li Cheng, Jue-Hua Jing
{"title":"Imatinib inhibits pericyte-fibroblast transition and inflammation and promotes axon regeneration by blocking the PDGF-BB/PDGFRβ pathway in spinal cord injury.","authors":"Fei Yao,&nbsp;Yang Luo,&nbsp;Yan-Chang Liu,&nbsp;Yi-Hao Chen,&nbsp;Yi-Teng Li,&nbsp;Xu-Yang Hu,&nbsp;Xing-Yu You,&nbsp;Shui-Sheng Yu,&nbsp;Zi-Yu Li,&nbsp;Lei Chen,&nbsp;Da-Sheng Tian,&nbsp;Mei-Ge Zheng,&nbsp;Li Cheng,&nbsp;Jue-Hua Jing","doi":"10.1186/s41232-022-00223-9","DOIUrl":"https://doi.org/10.1186/s41232-022-00223-9","url":null,"abstract":"<p><strong>Background: </strong>Fibrotic scar formation and inflammation are characteristic pathologies of spinal cord injury (SCI) in the injured core, which has been widely regarded as the main barrier to axonal regeneration resulting in permanent functional recovery failure. Pericytes were shown to be the main source of fibroblasts that form fibrotic scar. However, the mechanism of pericyte-fibroblast transition after SCI remains elusive.</p><p><strong>Methods: </strong>Fibrotic scarring and microvessels were assessed using immunofluorescence staining after establishing a crush SCI model. To study the process of pericyte-fibroblast transition, we analyzed pericyte marker and fibroblast marker expression using immunofluorescence. The distribution and cellular origin of platelet-derived growth factor (PDGF)-BB were examined with immunofluorescence. Pericyte-fibroblast transition was detected with immunohistochemistry and Western blot assays after PDGF-BB knockdown and blocking PDGF-BB/PDGFRβ signaling in vitro. Intrathecal injection of imatinib was used to selectively inhibit PDGF-BB/PDGFRβ signaling. The Basso mouse scale score and footprint analysis were performed to assess functional recovery. Subsequently, axonal regeneration, fibrotic scarring, fibroblast population, proliferation and apoptosis of PDGFRβ<sup>+</sup> cells, microvessel leakage, and the inflammatory response were assessed with immunofluorescence.</p><p><strong>Results: </strong>PDGFRβ<sup>+</sup> pericytes detached from the blood vessel wall and transitioned into fibroblasts to form fibrotic scar after SCI. PDGF-BB was mainly distributed in the periphery of the injured core, and microvascular endothelial cells were one of the sources of PDGF-BB in the acute phase. Microvascular endothelial cells induced pericyte-fibroblast transition through the PDGF-BB/PDGFRβ signaling pathway in vitro. Pharmacologically blocking the PDGF-BB/PDGFRβ pathway promoted motor function recovery and axonal regeneration and inhibited fibrotic scar formation. After fibrotic scar formation, blocking the PDGFRβ receptor inhibited proliferation and promoted apoptosis of PDGFRβ<sup>+</sup> cells. Imatinib did not alter pericyte coverage on microvessels, while microvessel leakage and inflammation were significantly decreased after imatinib treatment.</p><p><strong>Conclusions: </strong>We reveal that the crosstalk between microvascular endothelial cells and pericytes promotes pericyte-fibroblast transition through the PDGF-BB/PDGFRβ signaling pathway. Our finding suggests that blocking the PDGF-BB/PDGFRβ signaling pathway with imatinib contributes to functional recovery, fibrotic scarring, and inflammatory attenuation after SCI and provides a potential target for the treatment of SCI.</p>","PeriodicalId":13588,"journal":{"name":"Inflammation and Regeneration","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9511779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40375068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Enhancer RNA commits osteogenesis via microRNA-3129 expression in human bone marrow-derived mesenchymal stem cells. 增强子RNA在人骨髓间充质干细胞中通过microRNA-3129的表达实现成骨。
IF 8.1 3区 医学
Inflammation and Regeneration Pub Date : 2022-09-16 DOI: 10.1186/s41232-022-00228-4
Anh Phuong Nguyen, Kaoru Yamagata, Shigeru Iwata, Gulzhan Trimova, Tong Zhang, Yu Shan, Mai-Phuong Nguyen, Koshiro Sonomoto, Shingo Nakayamada, Shigeaki Kato, Yoshiya Tanaka
{"title":"Enhancer RNA commits osteogenesis via microRNA-3129 expression in human bone marrow-derived mesenchymal stem cells.","authors":"Anh Phuong Nguyen,&nbsp;Kaoru Yamagata,&nbsp;Shigeru Iwata,&nbsp;Gulzhan Trimova,&nbsp;Tong Zhang,&nbsp;Yu Shan,&nbsp;Mai-Phuong Nguyen,&nbsp;Koshiro Sonomoto,&nbsp;Shingo Nakayamada,&nbsp;Shigeaki Kato,&nbsp;Yoshiya Tanaka","doi":"10.1186/s41232-022-00228-4","DOIUrl":"https://doi.org/10.1186/s41232-022-00228-4","url":null,"abstract":"<p><strong>Background: </strong>Highly regulated gene expression program underlies osteogenesis of mesenchymal stem cells (MSCs), but the regulators in the program are not entirely identified. As enhancer RNAs (eRNAs) have recently emerged as a key regulator in gene expression, we assume a commitment of an eRNA in osteogenesis.</p><p><strong>Methods: </strong>We performed in silico analysis to identify potential osteogenic microRNA (miRNA) gene predicted to be regulated by super-enhancers (SEs). SE inhibitor treatment and eRNA knocking-down were used to confirm the regulational mechanism of eRNA. miRNA function in osteogenesis was elucidated by miR mimic and inhibitor transfection experiments.</p><p><strong>Results: </strong>miR-3129 was found to be located adjacent in a SE (osteoblast-specific SE_46171) specifically activated in osteoblasts by in silico analysis. A RT-quantitative PCR analysis of human bone marrow-derived MSC (hBMSC) cells showed that eRNA_2S was transcribed from the SE with the expression of miR-3129. Knockdown of eRNA_2S by locked nucleic acid as well as treatment of SE inhibitors JQ1 or THZ1 resulted in low miR-3129 levels. Overexpression of miR-3129 promoted hBMSC osteogenesis, while knockdown of miR-3129 inhibited hBMSC osteogenesis. Solute carrier family 7 member 11 (SLC7A11), encoding a bone formation suppressor, was upregulated following miR-3129-5p inhibition and identified as a target gene for miR-3129 during differentiation of hBMSCs into osteoblasts.</p><p><strong>Conclusions: </strong>miR-3129 expression is regulated by SEs via eRNA_2S and this miRNA promotes hBMSC differentiation into osteoblasts through downregulating the target gene SLC7A11. Thus, the present study uncovers a commitment of an eRNA via a miR-3129/SLC7A11 regulatory pathway during osteogenesis of hBMSCs.</p>","PeriodicalId":13588,"journal":{"name":"Inflammation and Regeneration","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40363985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Entering the era of precision medicine through multiomics approach. 通过多组学方法进入精准医疗时代。
IF 8.1 3区 医学
Inflammation and Regeneration Pub Date : 2022-09-09 DOI: 10.1186/s41232-022-00229-3
Keishi Fujio
{"title":"Entering the era of precision medicine through multiomics approach.","authors":"Keishi Fujio","doi":"10.1186/s41232-022-00229-3","DOIUrl":"https://doi.org/10.1186/s41232-022-00229-3","url":null,"abstract":"","PeriodicalId":13588,"journal":{"name":"Inflammation and Regeneration","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2022-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9461182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33456081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Helicobacter pylori infection in the stomach induces neuroinflammation: the potential roles of bacterial outer membrane vesicles in an animal model of Alzheimer's disease. 胃幽门螺杆菌感染诱发神经炎症:细菌外膜囊泡在阿尔茨海默病动物模型中的潜在作用。
IF 5 3区 医学
Inflammation and Regeneration Pub Date : 2022-09-05 DOI: 10.1186/s41232-022-00224-8
Ah-Mee Park, Ikuo Tsunoda
{"title":"Helicobacter pylori infection in the stomach induces neuroinflammation: the potential roles of bacterial outer membrane vesicles in an animal model of Alzheimer's disease.","authors":"Ah-Mee Park, Ikuo Tsunoda","doi":"10.1186/s41232-022-00224-8","DOIUrl":"10.1186/s41232-022-00224-8","url":null,"abstract":"<p><p>Helicobacter pylori (HP) is a Gram-negative bacterium that colonizes the human stomach chronically. Colonization of HP in the gastric mucosa not only causes gastrointestinal diseases, but also is associated with extra-gastric diseases, such as idiopathic thrombocytopenic purpura and neurological diseases. Among neurological diseases, epidemiological studies have shown that HP infection increases the prevalence of Alzheimer's disease (AD) and Parkinson's disease (PD). Since HP does not invade the central nervous system (CNS), it has been considered that systemic immunological changes induced by HP infection may play pathogenic roles in AD and PD. Here, we investigated the effects of HP infection on the CNS in vivo and in vitro. In the CNS, chronically HP-infected mice had microglial activation without HP colonization, although systemic immunological changes were not observed. This led us to explore the possibility that HP-derived outer membrane vesicles (HP-OMVs) could cause neuroinflammation. OMVs are small, spherical bilayer vesicles (20-500 nm) released into the extracellular space from the outer membrane of Gram-negative bacteria; OMVs contain lipopolysaccharide, proteins, peptidoglycan, DNA, and RNA. OMVs have also been shown to activate both innate and acquired immune cells in vitro, and to disrupt the tight junctions of the gastric epithelium (\"leaky gut\") as well as cross the blood-brain barrier in vivo. Thus, in theory, OMVs can activate immune responses in the remote organs, including the lymphoid organs and CNS, if only OMVs enter the systemic circulation. From the exosome fraction of sera from HP-infected mice, we detected HP-specific DNA, suggesting the presence of HP-OMVs. We also found that microglia incubated with HP-OMVs in vitro increased the cell proliferation, inflammatory cytokine production, and migration. On the other hand, HP-OMVs suppressed the cell proliferation of neuroblastoma in vitro. Lastly, we found that AD model mice infected with HP had amyloid plaques adjacent to activated microglia and astrocytes in vivo. Based on the literature review and our experimental data, we propose our working hypothesis that OMVs produced in chronic HP infection in the gut induce neuroinflammation in the CNS, explaining the higher prevalence of AD in HP-infected people.</p>","PeriodicalId":13588,"journal":{"name":"Inflammation and Regeneration","volume":null,"pages":null},"PeriodicalIF":5.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9442937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40348103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transforming growth factor-β-induced secretion of extracellular vesicles from oral cancer cells evokes endothelial barrier instability via endothelial-mesenchymal transition. 转化生长因子-β诱导的口腔癌细胞外囊泡分泌通过内皮-间质转化引起内皮屏障不稳定。
IF 8.1 3区 医学
Inflammation and Regeneration Pub Date : 2022-09-04 DOI: 10.1186/s41232-022-00225-7
Miho Kobayashi, Kashio Fujiwara, Kazuki Takahashi, Yusuke Yoshioka, Takahiro Ochiya, Katarzyna A Podyma-Inoue, Tetsuro Watabe
{"title":"Transforming growth factor-β-induced secretion of extracellular vesicles from oral cancer cells evokes endothelial barrier instability via endothelial-mesenchymal transition.","authors":"Miho Kobayashi,&nbsp;Kashio Fujiwara,&nbsp;Kazuki Takahashi,&nbsp;Yusuke Yoshioka,&nbsp;Takahiro Ochiya,&nbsp;Katarzyna A Podyma-Inoue,&nbsp;Tetsuro Watabe","doi":"10.1186/s41232-022-00225-7","DOIUrl":"https://doi.org/10.1186/s41232-022-00225-7","url":null,"abstract":"<p><strong>Background: </strong>During metastasis, cancer cells undergo epithelial-mesenchymal transition (EMT) in response to transforming growth factor-β (TGF-β), which is abundant in the tumor microenvironment, and acquire invasive and metastatic potentials. Metastasis to distant organs requires intravascular invasion and extravasation of cancer cells, which is accompanied by the disruption of the adhesion between vascular endothelial cells. Cancer cell-derived extracellular vesicles (EVs) have been suggested to induce the destabilization of normal blood vessels at the metastatic sites. However, the roles of EVs secreted from cancer cells that have undergone EMT in the destabilization of blood vessels remain to be elucidated. In the present study, we characterized EVs secreted by oral cancer cells undergoing TGF-β-induced EMT and elucidated their effects on the characteristics of vascular endothelial cells.</p><p><strong>Methods: </strong>Induction of EMT by TGF-β in human oral cancer cells was assessed using quantitative RT-PCR (qRT-PCR) and immunocytochemistry. Oral cancer cell-derived EVs were isolated from the conditioned media of oral cancer cells that were treated with or without TGF-β using ultracentrifugation, and characterized using nanoparticle tracking analysis and immunoblotting. The effects of EVs on human umbilical artery endothelial cells were examined by qRT-PCR, cellular staining, and permeability assay. The significant differences between means were determined using a t-test or one-way analysis of variance with Tukey's multiple comparisons test.</p><p><strong>Results: </strong>Oral cancer cells underwent EMT in response to TGF-β as revealed by changes in the expression of epithelial and mesenchymal cell markers at both the RNA and protein levels. Oral cancer cells treated with TGF-β showed increased EV production and altered EV composition when compared with untreated cells. The EVs that originated from cells that underwent EMT by TGF-β induced endothelial-mesenchymal transition, which was characterized by the decreased and increased expression of endothelial and mesenchymal cell markers, respectively. EVs derived from oral cancer cells also induced intercellular gap formation which led to the loss of endothelial cell barrier stability.</p><p><strong>Conclusions: </strong>EVs released from oral cancer cells that underwent TGF-β-induced EMT target endothelial cells to induce vascular destabilization. Detailed characterization of oral cancer-derived EVs and factors responsible for EV-mediated vascular instability will lead to the development of agents targeting metastasis.</p>","PeriodicalId":13588,"journal":{"name":"Inflammation and Regeneration","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2022-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40345849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Thymic self-antigen expression for immune tolerance and surveillance. 胸腺自身抗原表达对免疫耐受和监测的影响。
IF 8.1 3区 医学
Inflammation and Regeneration Pub Date : 2022-09-03 DOI: 10.1186/s41232-022-00211-z
Rayene Benlaribi, Qiao Gou, Hiroyuki Takaba
{"title":"Thymic self-antigen expression for immune tolerance and surveillance.","authors":"Rayene Benlaribi,&nbsp;Qiao Gou,&nbsp;Hiroyuki Takaba","doi":"10.1186/s41232-022-00211-z","DOIUrl":"https://doi.org/10.1186/s41232-022-00211-z","url":null,"abstract":"<p><p>T cells are a group of lymphocytes that play a central role in the immune system, notably, eliminating pathogens and attacking cancer while being tolerant of the self. Elucidating how immune tolerance is ensured has become a significant research issue for understanding the pathogenesis of autoimmune diseases as well as cancer immunity. T cell immune tolerance is established mainly in the thymic medulla by the removal of self-responsive T cells and the generation of regulatory T cells, this process depends mainly on the expression of a variety of tissue restricted antigens (TRAs) by medullary thymic epithelial cells (mTECs). The expression of TRAs is known to be regulated by at least two independent factors, Fezf2 and Aire, which play non-redundant and complementary roles by different mechanisms. In this review, we introduce the molecular logic of thymic self-antigen expression that underlies T cell selection for the prevention of autoimmunity and the establishment of immune surveillance.</p>","PeriodicalId":13588,"journal":{"name":"Inflammation and Regeneration","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2022-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40346512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Osteoclast biology in the single-cell era. 单细胞时代的破骨细胞生物学。
IF 8.1 3区 医学
Inflammation and Regeneration Pub Date : 2022-09-02 DOI: 10.1186/s41232-022-00213-x
Masayuki Tsukasaki, Hiroshi Takayanagi
{"title":"Osteoclast biology in the single-cell era.","authors":"Masayuki Tsukasaki,&nbsp;Hiroshi Takayanagi","doi":"10.1186/s41232-022-00213-x","DOIUrl":"https://doi.org/10.1186/s41232-022-00213-x","url":null,"abstract":"<p><p>Osteoclasts, the only cells that can resorb bone, play a central role in bone homeostasis as well as bone damage under pathological conditions such as osteoporosis, arthritis, periodontitis, and bone metastasis. Recent studies using single-cell technologies have uncovered the regulatory mechanisms underlying osteoclastogenesis at unprecedented resolution and shed light on the possibility that there is heterogeneity in the origin, function, and fate of osteoclast-lineage cells. Here, we discuss the current advances and emerging concepts in osteoclast biology.</p>","PeriodicalId":13588,"journal":{"name":"Inflammation and Regeneration","volume":null,"pages":null},"PeriodicalIF":8.1,"publicationDate":"2022-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9438068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40341592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Controlling skin microbiome as a new bacteriotherapy for inflammatory skin diseases. 控制皮肤微生物群是治疗炎症性皮肤病的一种新的细菌疗法。
IF 8.1 3区 医学
Inflammation and Regeneration Pub Date : 2022-09-01 DOI: 10.1186/s41232-022-00212-y
Yoshihiro Ito, Masayuki Amagai
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引用次数: 12
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