Immunogenetics最新文献_第7页

Comparative genomics of the T cell receptor μ locus in marsupials and monotremes. 有袋动物和单孔目动物T细胞受体μ基因座的比较基因组学。

IF 2.9 4区医学

Immunogenetics Pub Date : 2023-12-01 Epub Date: 2023-09-25 DOI: 10.1007/s00251-023-01320-w

K A Morrissey, M R Stammnitz, E Murchison, R D Miller

引用次数: 0

Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania. 免疫遗传学，森林瘟疫及其媒介：坦桑尼亚纳他氏Mastomys natalensis病原体库的见解。

IF 2.9 4区医学

Immunogenetics Pub Date : 2023-12-01 Epub Date: 2023-10-19 DOI: 10.1007/s00251-023-01323-7

Lavinia Haikukutu, Japhet R Lyaku, Charles M Lyimo, Seth J Eiseb, Rhodes H Makundi, Ayodeji Olayemi, Kerstin Wilhelm, Nadine Müller-Klein, Dominik W Schmid, Ramona Fleischer, Simone Sommer

{"title":"Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in Tanzania.","authors":"Lavinia Haikukutu, Japhet R Lyaku, Charles M Lyimo, Seth J Eiseb, Rhodes H Makundi, Ayodeji Olayemi, Kerstin Wilhelm, Nadine Müller-Klein, Dominik W Schmid, Ramona Fleischer, Simone Sommer","doi":"10.1007/s00251-023-01323-7","DOIUrl":"10.1007/s00251-023-01323-7","url":null,"abstract":"Yersinia pestis is a historically important vector-borne pathogen causing plague in humans and other mammals. Contemporary zoonotic infections with Y. pestis still occur in sub-Saharan Africa, including Tanzania and Madagascar, but receive relatively little attention. Thus, the role of wildlife reservoirs in maintaining sylvatic plague and spillover risks to humans is largely unknown. The multimammate rodent Mastomys natalensis is the most abundant and widespread rodent in peri-domestic areas in Tanzania, where it plays a major role as a Y. pestis reservoir in endemic foci. Yet, how M. natalensis' immunogenetics contributes to the maintenance of plague has not been investigated to date. Here, we surveyed wild M. natalensis for Y. pestis vectors, i.e., fleas, and tested for the presence of antibodies against Y. pestis using enzyme-linked immunosorbent assays (ELISA) in areas known to be endemic or without previous records of Y. pestis in Tanzania. We characterized the allelic and functional (i.e., supertype) diversity of the major histocompatibility complex (MHC class II) of M. natalensis and investigated links to Y. pestis vectors and infections. We detected antibodies against Y. pestis in rodents inhabiting both endemic areas and areas considered non-endemic. Of the 111 nucleotide MHC alleles, only DRB*016 was associated with an increased infestation with the flea Xenopsylla. Surprisingly, we found no link between MHC alleles or supertypes and antibodies of Y. pestis. Our findings hint, however, at local adaptations towards Y. pestis vectors, an observation that more exhaustive sampling could unwind in the future.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":" ","pages":"517-530"},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49677109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The KIR2DL1 intermediate upstream element participates in gene activation. KIR2DL1中间上游元件参与基因激活。

IF 2.9 4区医学

Immunogenetics Pub Date : 2023-12-01 Epub Date: 2023-10-06 DOI: 10.1007/s00251-023-01321-9

Paul W Wright, Hongchuan Li, Md Ahasanur Rahman, Erik M Anderson, Megan Karwan, Jeffrey Carrell, Stephen K Anderson

{"title":"The KIR2DL1 intermediate upstream element participates in gene activation.","authors":"Paul W Wright, Hongchuan Li, Md Ahasanur Rahman, Erik M Anderson, Megan Karwan, Jeffrey Carrell, Stephen K Anderson","doi":"10.1007/s00251-023-01321-9","DOIUrl":"10.1007/s00251-023-01321-9","url":null,"abstract":"The human KIR genes encode a family of class I MHC receptors that are expressed on subsets of NK cells. The expression of KIR proteins is controlled by a stochastic process, and competition between sense and antisense promoter elements has been suggested to program the variegated expression of these genes. Previous studies have demonstrated distinct roles of distal, intermediate, and proximal sense promoter/enhancer elements in gene activation and expression. Conversely, proximal and intronic antisense promoter transcripts have been associated with gene silencing at different stages of NK cell development. In the current study, we examine the effect of intermediate promoter deletion on KIR2DL1 expression in the YTS cell line. Homozygous deletion of the KIR2DL1 intermediate element did not affect proximal promoter activity but resulted in increased detection of upstream transcripts. No significant changes in alternative mRNA splicing or expression levels of KIR2DL1 protein were observed. However, intermediate element deletion was associated with a reduced frequency of gene activation by 5-azacytidine. Taken together, these results indicate that the intermediate element is not an enhancer required for KIR expression; however, it is required for the efficient activation of the gene.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":" ","pages":"495-506"},"PeriodicalIF":2.9,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41129347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased circulating Th17 cell populations in patients with pancreatic ductal adenocarcinoma. 胰腺导管腺癌患者循环Th17细胞群增加。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-10-01 Epub Date: 2023-08-04 DOI: 10.1007/s00251-023-01318-4

Imteyaz Ahmad Khan, Nidhi Singh, Deepak Gunjan, Srikant Gopi, Nihar Ranjan Dash, Surabhi Gupta, Anoop Saraya

{"title":"Increased circulating Th17 cell populations in patients with pancreatic ductal adenocarcinoma.","authors":"Imteyaz Ahmad Khan, Nidhi Singh, Deepak Gunjan, Srikant Gopi, Nihar Ranjan Dash, Surabhi Gupta, Anoop Saraya","doi":"10.1007/s00251-023-01318-4","DOIUrl":"10.1007/s00251-023-01318-4","url":null,"abstract":"T-helper 17 (Th17) cells are a subset of CD4+ helper T cells that produce interleukin 17 (IL-17) and play a crucial role in the pathogenesis of inflammatory and autoimmune diseases. Few studies have been conducted to determine the role of Th17 cells in the tumorigenesis and development of pancreatic ductal adenocarcinoma (PDAC); however, its role is still unclear. In this study, the percentage of circulating Th17 cells and serum levels of IL-17A and IL-23 were analyzed using flow cytometry and ELISA, respectively, in 40 PDAC patients, 30 chronic pancreatitis (CP) patients and 30 healthy controls (HC). In addition, the mRNA expression levels of IL-17A, STAT3 and RORγt in tissue samples were quantified by qRT-PCR. The results showed that the percentage of circulating Th17 cells and the concentrations of serum IL-17A and IL-23 were significantly increased in PDAC patients as compared to CP and HC (P < 0.001). In addition, the higher level of IL-17A was significantly correlated with the poor overall survival of the PDAC patients. Furthermore, the frequencies of Th17 cells and IL-17A were significantly higher in stage III+IV PDAC patients versus stage I+II. A significant increase in IL-17A, STAT3 and RORγT mRNA was observed in patients with PDAC. Taken together, these findings suggest that the increased circulating Th17 cells and serum IL-17A may be involved in the development and metastasis of PDAC, and thus represent potential targets for the treatment of PDAC.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":" ","pages":"433-443"},"PeriodicalIF":3.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9935394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correlation between human leukocyte antigen ligands and killer cell immunoglobulin-like receptors in aplastic anemia patients from Shaanxi Han. 陕西汉族再生障碍性贫血患者白细胞抗原配体与杀伤细胞免疫球蛋白样受体的相关性研究。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-10-01 Epub Date: 2023-08-17 DOI: 10.1007/s00251-023-01316-6

Tianju Wang, Jun Qi, Manni Wang, Hua Xu, Junhua Wu, Lixia Shang, Le Chen, Yuhui Li

{"title":"Correlation between human leukocyte antigen ligands and killer cell immunoglobulin-like receptors in aplastic anemia patients from Shaanxi Han.","authors":"Tianju Wang, Jun Qi, Manni Wang, Hua Xu, Junhua Wu, Lixia Shang, Le Chen, Yuhui Li","doi":"10.1007/s00251-023-01316-6","DOIUrl":"10.1007/s00251-023-01316-6","url":null,"abstract":"Regulating natural killer (NK) cell responses in hematological malignancies largely depend on molecular interactions between killer cell immunoglobulin-like receptors (KIR) and human leukocyte antigen (HLA) class I ligands. The goal of the current study was to examine the key functions of KIR genes, gene combinations of KIR-HLA, and KIR genotypes in genetic predisposition to aplastic anemia (AA). Herein, the genotyping of 16 KIR genes and HLA-A, -B, and -C ligands were performed in 72 AA patients and 150 healthy controls using PCR evaluations with sequence-specific primers using standard assays. According to the obtained results, AA patients had an increased incidence of activating KIR and KIR2DS4 (P = 0.465 × 10-4, Pc = 0.837 × 10-3, OR = 20.81, 95% CI = 2.786-155.5) compared to controls. KIR/HLA class I ligand profile KIR2DS4/C1 (P = 0.350 × 10-4, Pc = 0.630 × 10-3, OR = 8.944, 95% CI = 2.667-29.993) was significantly elevated in AA patients compared to healthy controls. Genotype AA1 (P = 0.003, OR = 2.351, 95% CI = 1.325-4.172) were increased, and AA195 (P = 0.006, OR = 0.060, 95% CI = 0.004-1.023) was decreased among AA cases compared to controls. Our findings indicated that KIR2DS4 may play a role in the pathogenesis of AA. This study revealed the contribution of KIR genes in the etiology of AA cases.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":" ","pages":"445-454"},"PeriodicalIF":3.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10020785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors regulating the differences in frequency of infiltration of Th17 and Treg of the blood-brain barrier. 调节血脑屏障Th17和Treg浸润频率差异的因素。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-10-01 Epub Date: 2023-07-11 DOI: 10.1007/s00251-023-01310-y

Norwin Kubick, Marzena Lazarczyk, Nina Strzałkowska, Anna Charuta, Jarosław Olav Horbańczuk, Mariusz Sacharczuk, Michel Edwar Mickael

{"title":"Factors regulating the differences in frequency of infiltration of Th17 and Treg of the blood-brain barrier.","authors":"Norwin Kubick, Marzena Lazarczyk, Nina Strzałkowska, Anna Charuta, Jarosław Olav Horbańczuk, Mariusz Sacharczuk, Michel Edwar Mickael","doi":"10.1007/s00251-023-01310-y","DOIUrl":"10.1007/s00251-023-01310-y","url":null,"abstract":"Controlling CD4+ immune cell infiltration of the brain is a leading aim in designing therapeutic strategies for a range of neuropathological disorders such as multiple sclerosis, Alzheimer's disease, and depression. CD4+ T cells are a highly heterogeneous and reprogrammable family, which includes various distinctive cell types such as Th17, Th1, and Treg cells. Interestingly Th17 and Treg cells share a related transcriptomic profile, where the TGFβ-SMADS pathway plays a fundamental role in regulating the differentiation of both of these cell types. However, Th17 could be highly pathogenic and was shown to promote inflammation in various neuropathological disorders. Conversely, Treg is anti-inflammatory and is known to inhibit Th17. It could be noticed that Th17 frequencies of infiltration of the blood-brain barrier in various neurological disorders are significantly upregulated. However, Treg infiltration numbers are significantly low. The reasons behind these contradicting observations are still unknown. In this perspective, we propose that the difference in the T-cell receptor repertoire diversity, diapedesis pathways, chemokine expression, and mechanical properties of these two cell types could be contributing to answering this intriguing question.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":" ","pages":"417-423"},"PeriodicalIF":3.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10143797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dysregulated PD-L2 is correlated with disease activity and inflammation in rheumatoid arthritis. PD-L2失调与类风湿性关节炎的疾病活动和炎症相关。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-10-01 Epub Date: 2023-07-05 DOI: 10.1007/s00251-023-01307-7

Jian Xiong, Jie Yang, Yuling Sun, Yachun Chen, Yundi Guo, Cuiping Liu, Jing Sun

{"title":"Dysregulated PD-L2 is correlated with disease activity and inflammation in rheumatoid arthritis.","authors":"Jian Xiong, Jie Yang, Yuling Sun, Yachun Chen, Yundi Guo, Cuiping Liu, Jing Sun","doi":"10.1007/s00251-023-01307-7","DOIUrl":"10.1007/s00251-023-01307-7","url":null,"abstract":"The programmed death-1 (PD-1) pathway has been shown to deliver an inhibitory signal, and aberrant expression of the PD-1 molecule and/or its ligand programmed death ligand 1 (PD-L1) has been demonstrated in human diseases, while its other ligand, programmed death ligand 2 (PD-L2), has rarely been studied. Here, we investigated the expression of PD-L2 in synovial tissue and blood from patients with rheumatoid arthritis (RA). Soluble PD-L2 and inflammatory cytokine levels in serum among healthy controls and patients with RA were compared via enzyme-linked immunosorbent assay (ELISA). Membrane PD-L2 on monocytes in blood was analyzed through flow cytometry (FCM). The different expression levels of PD-L2 between the RA and non-RA synovium were semi-quantified by immunohistochemical (IHC) staining. The soluble PD-L2 levels in serum from patients with RA were significantly lower than those in healthy subjects, correlating with active parameters (rheumatoid factor) and inflammatory cytokine secretion. The FCM results showed that patients with RA had significantly increased percentages of PD-L2-expressing CD14+ monocytes and correlated with inflammatory cytokines. PD-L2 expression on macrophages in the synovium from patients with RA was recorded by IHC staining with a higher score, and its correlation with pathological scores and clinical features was determined. Together, our results revealed aberrant expression of PD-L2 in RA, which may be a promising biomarker and therapeutic target associated with the pathogenesis of RA.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":" ","pages":"425-431"},"PeriodicalIF":3.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10110767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prof. Dr. Jan Klein (1936-2023). Jan Klein教授博士（1936-2023）。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-10-01 DOI: 10.1007/s00251-023-01317-5

Ronald Bontrop, Martin Flajnik, Colm S O'Huigin, Masanori Kasahara

引用次数: 0

Ancient fish lineages illuminate toll-like receptor diversification in early vertebrate evolution. 古代鱼类谱系阐明了早期脊椎动物进化中toll样受体的多样性。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-10-01 Epub Date: 2023-08-09 DOI: 10.1007/s00251-023-01315-7

Kara B Carlson, Cameron Nguyen, Dustin J Wcisel, Jeffrey A Yoder, Alex Dornburg

{"title":"Ancient fish lineages illuminate toll-like receptor diversification in early vertebrate evolution.","authors":"Kara B Carlson, Cameron Nguyen, Dustin J Wcisel, Jeffrey A Yoder, Alex Dornburg","doi":"10.1007/s00251-023-01315-7","DOIUrl":"10.1007/s00251-023-01315-7","url":null,"abstract":"Since its initial discovery over 50 years ago, understanding the evolution of the vertebrate RAG- mediated adaptive immune response has been a major area of research focus for comparative geneticists. However, how the evolutionary novelty of an adaptive immune response impacted the diversity of receptors associated with the innate immune response has received considerably less attention until recently. Here, we investigate the diversification of vertebrate toll-like receptors (TLRs), one of the most ancient and well conserved innate immune receptor families found across the Tree of Life, integrating genomic data that represent all major vertebrate lineages with new transcriptomic data from Polypteriformes, the earliest diverging ray-finned fish lineage. Our analyses reveal TLR sequences that reflect the 6 major TLR subfamilies, TLR1, TLR3, TLR4, TLR5, TLR7, and TLR11, and also currently unnamed, yet phylogenetically distinct TLR clades. We additionally recover evidence for a pulse of gene gain coincident with the rise of the RAG-mediated adaptive immune response in jawed vertebrates, followed by a period of rapid gene loss during the Cretaceous. These gene losses are primarily concentrated in marine teleost fish and synchronous with the mid Cretaceous anoxic event, a period of rapid extinction for marine species. Finally, we reveal a mismatch between phylogenetic placement and gene nomenclature for up to 50% of TLRs found in clades such as ray-finned fishes, cyclostomes, amphibians, and elasmobranchs. Collectively, these results provide an unparalleled perspective of TLR diversity and offer a ready framework for testing gene annotations in non-model species.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":" ","pages":"465-478"},"PeriodicalIF":3.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9960738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The minor chicken class I gene BF1 is deleted between short imperfect direct repeats in the B14 and typical B15 major histocompatibility complex (MHC) haplotypes. 次要的鸡I类基因BF1在B14和典型的B15主要组织相容性复合体（MHC）单倍型中的短不完全直接重复之间缺失。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-10-01 Epub Date: 2023-07-05 DOI: 10.1007/s00251-023-01313-9

Nicolas I E Rocos, Felicity J Coulter, Thomas C J Tan, Jim Kaufman

{"title":"The minor chicken class I gene BF1 is deleted between short imperfect direct repeats in the B14 and typical B15 major histocompatibility complex (MHC) haplotypes.","authors":"Nicolas I E Rocos, Felicity J Coulter, Thomas C J Tan, Jim Kaufman","doi":"10.1007/s00251-023-01313-9","DOIUrl":"10.1007/s00251-023-01313-9","url":null,"abstract":"The chicken major histocompatibility complex (MHC, also known as the BF-BL region of the B locus) is notably small and simple with few genes, most of which are involved in antigen processing and presentation. There are two classical class I genes, of which only BF2 is well and systemically expressed as the major ligand for cytotoxic T lymphocytes (CTLs). The other class I gene, BF1, is believed to be primarily a natural killer (NK) cell ligand. Among most standard chicken MHC haplotypes examined in detail, BF1 is expressed tenfold less than BF2 at the RNA level due to defects in the promoter or in a splice site. However, in the B14 and typical B15 haplotypes, BF1 RNA was not detected, and here, we show that a deletion between imperfect 32 nucleotide direct repeats has removed the BF1 gene entirely. The phenotypic effects of not having a BF1 gene (particularly on resistance to infectious pathogens) have not been systematically explored, but such deletions between short direct repeats are also found in some BF1 promoters and in the 5' untranslated region (5'UTR) of some BG genes found in the BG region of the B locus. Despite the opposite transcriptional orientation of homologous genes in the chicken MHC, which might prevent the loss of key genes from a minimal essential MHC, it appears that small direct repeats can still lead to deletion.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":" ","pages":"455-464"},"PeriodicalIF":3.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10514180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10110766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0