Immunogenetics最新文献_第10页

Correction to: Llama peripheral B-cell populations producing conventional and heavy chain-only IgG subtypes are phenotypically indistinguishable but immunogenetically distinct. 修正:产生常规和仅重链IgG亚型的羊驼外周b细胞群在表型上无法区分，但在免疫遗传学上是不同的。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-04-01 DOI: 10.1007/s00251-023-01299-4

Kevin A Henry, Henk van Faassen, Doreen Harcus, Anne Marcil, Jennifer J Hill, Serge Muyldermans, C Roger MacKenzie

引用次数: 0

Unfavorable immunotherapy plus tyrosine kinase inhibition outcome of metastatic renal cell carcinoma after radical nephrectomy with increased ADAM9 expression. 不良免疫治疗加酪氨酸激酶抑制转移性肾癌根治性肾切除术后ADAM9表达升高的结果。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-04-01 DOI: 10.1007/s00251-022-01292-3

Xianglai Xu, Ying Wang, Zhaoyi Chen, Yanjun Zhu, Jiajun Wang, Jianming Guo

{"title":"Unfavorable immunotherapy plus tyrosine kinase inhibition outcome of metastatic renal cell carcinoma after radical nephrectomy with increased ADAM9 expression.","authors":"Xianglai Xu, Ying Wang, Zhaoyi Chen, Yanjun Zhu, Jiajun Wang, Jianming Guo","doi":"10.1007/s00251-022-01292-3","DOIUrl":"https://doi.org/10.1007/s00251-022-01292-3","url":null,"abstract":"Immunotherapy plus tyrosine kinase inhibitor (IO-TKI) has become the standard first-line therapy for advanced renal cell carcinoma (RCC). However, the modest response rate of IO-TKI therapy and the absence of biomarkers limited the selection of treatment strategies for RCC patients. There were three cohorts enrolled: two from our facility (ZS-MRCC and ZS-HRRCC) and one from a clinical study (JAVELIN-101). By RNA sequencing, the expression of ADAM9 in each sample was measured. By flow cytometry and immunohistochemistry, immune infiltration and T cell function were examined. Primary outcomes were established as treatment response and progression-free survival (PFS). Patients with low-ADAM9 expression had a higher objective response rate (56.5% vs 13.6%, P = 0.01) and longer PFS in both cohorts. In the ZS-HRRCC cohort, the expression of ADAM9 was associated with increased tumor-infiltrating T cells, which was proved by immunohistochemistry (P < 0.05) and flow cytometry (Spearman's ρ = 0.42, P < 0.001). In the high-ADAM9 group, CD8+ and CD4+ T cells revealed an exhausted phenotype with decreased GZMB (Spearman's ρ = - 0.31, P = 0.05, and Spearman's ρ = - 0.49, P < 0.001, respectively), and fewer Macrophages were identified. A predictive RFscore was further constructed by random forest approach, involving ADAM9 and immunologic genes. Only in the subgroup with the lower RFscore did IO-TKI outperform TKI monotherapy. High-ADAM9 expression was associated with immunosuppression and IO-TKI resistance. Expression of ADAM9 was also associated with the exhaustion and dysfunction of T cells. ADAM9-based RFscore has the potential to be used as a biomarker to distinguish the optimal patient treatment methods between IO-TKI and TKI monotherapy.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":"75 2","pages":"133-143"},"PeriodicalIF":3.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9201482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IL4I1 enhances PD-L1 expression through JAK/STAT signaling pathway in lung adenocarcinoma. IL4I1通过JAK/STAT信号通路增强PD-L1在肺腺癌中的表达。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-02-01 DOI: 10.1007/s00251-022-01275-4

Jiefei Zhu, Yan Li, Xu Lv

{"title":"IL4I1 enhances PD-L1 expression through JAK/STAT signaling pathway in lung adenocarcinoma.","authors":"Jiefei Zhu, Yan Li, Xu Lv","doi":"10.1007/s00251-022-01275-4","DOIUrl":"https://doi.org/10.1007/s00251-022-01275-4","url":null,"abstract":"Lung adenocarcinoma (LUAD) is the major type of lung cancer and is one of the deadliest cancers worldwide. IL4I1, as a gene associated with unsatisfactory prognosis, is involved in tumor immune escape, but its immune regulatory mechanism in LUAD is limited. Bioinformatics analysis was applied to analyze the differentially expressed mRNAs and enriched signaling pathways in LUAD tissue. Quantitative real-time polymerase chain reaction (qRT-PCR) was manipulated to test IL4I1 expression. We carried out several methods to examine cell functions: CCK-8 to measure LUAD cell proliferation; flow cytometry to determine cell apoptosis; Western blot to assess the expression of JAK/STAT pathway-related proteins and PD-L1; T cell cytotoxicity assay to evaluate the effect of IL4I1 on the immune escape of LUAD cells. Through bioinformatics analysis, IL4I1 was verified to be highly expressed in LUAD tissue, participate in the modulation of JAK/STAT signaling pathway, and be positively associated with CD274 (PD-L1) expression. Cell function experiments indicated that silencing IL4I1 notably repressed LUAD cell proliferation and induced apoptosis. IL4I1 silence would block JAK/STAT signaling pathway, but this effect could be reversed by RO8191 activator treatment. Moreover, IL4I1 silence suppressed PD-L1 expression and facilitated T cell cytotoxicity, while its inhibitory impact on PD-L1 expression and immune escape of LUAD cells could be reversed by atezolizumab treatment. Overall, we confirmed that IL4I1 promoted the malignant cell behaviors and immune escape of LUAD through JAK/STAT signaling pathway. IL4I1 has the potential to be a diagnostic biomarker for LUAD.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":"75 1","pages":"17-25"},"PeriodicalIF":3.2,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10698833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Functional consequences of allotypic polymorphisms in human immunoglobulin G subclasses. 人类免疫球蛋白 G 亚类异型多态性的功能性后果。

IF 2.9 4区医学

Immunogenetics Pub Date : 2023-02-01 Epub Date: 2022-07-29 DOI: 10.1007/s00251-022-01272-7

Andrew R Crowley, Simone I Richardson, Marina Tuyishime, Madeleine Jennewein, Meredith J Bailey, Jiwon Lee, Galit Alter, Guido Ferrari, Lynn Morris, Margaret E Ackerman

引用次数: 0

Mutated Pkhd1 alone is sufficient to cause autoimmune biliary disease on the nonobese diabetic (NOD) genetic background. 在非肥胖糖尿病（NOD）遗传背景下，仅突变的 Pkhd1 就足以导致自身免疫性胆道疾病。

IF 3.2 4区医学

Immunogenetics Pub Date : 2023-02-01 Epub Date: 2022-09-13 DOI: 10.1007/s00251-022-01276-3

David E Adams, Luke S Heuer, Manuel Rojas, Weici Zhang, William M Ridgway

{"title":"Mutated Pkhd1 alone is sufficient to cause autoimmune biliary disease on the nonobese diabetic (NOD) genetic background.","authors":"David E Adams, Luke S Heuer, Manuel Rojas, Weici Zhang, William M Ridgway","doi":"10.1007/s00251-022-01276-3","DOIUrl":"10.1007/s00251-022-01276-3","url":null,"abstract":"We previously reported that nonobese diabetic (NOD) congenic mice (NOD.c3c4 mice) developed an autoimmune biliary disease (ABD) with similarities to human primary biliary cholangitis (PBC), including anti-mitochondrial antibodies and organ-specific biliary lymphocytic infiltrates. We narrowed the possible contributory regions in a novel NOD.Abd3 congenic mouse to a B10 congenic region on chromosome 1 (\"Abd3\") and a mutated Pkhd1 gene (Pkhd1del36-67) upstream from Abd3, and we showed via backcrossing studies that the NOD genetic background was necessary for disease. Here, we show that NOD.Abd3 mice develop anti-PDC-E2 autoantibodies at high levels, and that placing the chromosome 1 interval onto a scid background eliminates disease, demonstrating the critical role of the adaptive immune system in pathogenesis. While the NOD genetic background is essential for disease, it was still unclear which of the two regions in the Abd3 locus were necessary and sufficient for disease. Here, using a classic recombinant breeding approach, we prove that the mutated Pkhd1del36-67 alone, on the NOD background, causes ABD. Further characterization of the mutant sequence demonstrated that the Pkhd1 gene is disrupted by an ETnII-beta retrotransposon inserted in intron 35 in an anti-sense orientation. Homozygous Pkhd1 mutations significantly affect viability, with the offspring skewed away from a Mendelian distribution towards NOD Pkhd1 homozygous or heterozygous genotypes. Cell-specific abnormalities, on a susceptible genetic background, can therefore induce an organ-specific autoimmunity directed to the affected cells. Future work will aim to characterize how mutant Pkhd1 can cause such an autoimmune response.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":"75 1","pages":"27-37"},"PeriodicalIF":3.2,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9468241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10704640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SNHG3 regulates NEIL3 via transcription factor E2F1 to mediate malignant proliferation of hepatocellular carcinoma. SNHG3通过转录因子E2F1调控NEIL3介导肝细胞癌恶性增殖。

IF 2.9 4区医学

Immunogenetics Pub Date : 2023-02-01 Epub Date: 2022-09-17 DOI: 10.1007/s00251-022-01277-2

Fabiao Zhang, Jie Lu, Jian Yang, Qiqiang Dai, Xuefeng Du, Yongfu Xu, Caiming Zhang

{"title":"SNHG3 regulates NEIL3 via transcription factor E2F1 to mediate malignant proliferation of hepatocellular carcinoma.","authors":"Fabiao Zhang, Jie Lu, Jian Yang, Qiqiang Dai, Xuefeng Du, Yongfu Xu, Caiming Zhang","doi":"10.1007/s00251-022-01277-2","DOIUrl":"10.1007/s00251-022-01277-2","url":null,"abstract":"The involvement of small nucleolar RNA host gene 3 (SNHG3) in cancer regulation has been reported. This study attempted to deeply investigate the molecular regulatory mechanism of SNHG3 on malignant progression of hepatocellular carcinoma (HCC). According to TCGA analysis, high SNHG3 expression was a risk factor for poor prognosis of HCC patients. Therefore, we further detected the mRNA level of SNHG3 in HCC tissue and cells. It was found that SNHG3 was upregulated in HCC tissue and cells. Afterwards, CCK-8 and flow cytometry assays further proved that silencing SNHG3 inhibited HCC cell proliferation while inducing cell apoptosis and G0/G1 phase arrest. It was also attested in vivo experiments that silencing SNHG3 could reduce the volume and weight of tumors and downregulate the Ki-67 expression to suppress HCC tumor growth. Next, it was discovered that SNHG3 increased the binding of E2F1 and NEIL3 promoter region, thereby activating the transcription feature of NEIL3. Lastly, rescue assays indicated that NEIL3 participated in SNHG3-mediated HCC cell cycle, apoptosis and proliferation. All in all, this study revealed the specific regulatory mechanism of SNHG3 in HCC to enable SNHG3 a hopeful marker for HCC diagnosis and treatment.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":"75 1","pages":"39-51"},"PeriodicalIF":2.9,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10688998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A highly diverse set of novel immunoglobulin-like transcript (NILT) genes in zebrafish indicates a wide range of functions with complex relationships to mammalian receptors. 斑马鱼中高度多样化的新型免疫球蛋白样转录本（NILT）基因表明，它们具有与哺乳动物受体关系复杂的多种功能。

IF 2.9 4区医学

Immunogenetics Pub Date : 2023-02-01 Epub Date: 2022-07-23 DOI: 10.1007/s00251-022-01270-9

Dustin J Wcisel, Alex Dornburg, Sean C McConnell, Kyle M Hernandez, Jorge Andrade, Jill L O de Jong, Gary W Litman, Jeffrey A Yoder

{"title":"A highly diverse set of novel immunoglobulin-like transcript (NILT) genes in zebrafish indicates a wide range of functions with complex relationships to mammalian receptors.","authors":"Dustin J Wcisel, Alex Dornburg, Sean C McConnell, Kyle M Hernandez, Jorge Andrade, Jill L O de Jong, Gary W Litman, Jeffrey A Yoder","doi":"10.1007/s00251-022-01270-9","DOIUrl":"10.1007/s00251-022-01270-9","url":null,"abstract":"Multiple novel immunoglobulin-like transcripts (NILTs) have been identified from salmon, trout, and carp. NILTs typically encode activating or inhibitory transmembrane receptors with extracellular immunoglobulin (Ig) domains. Although predicted to provide immune recognition in ray-finned fish, we currently lack a definitive framework of NILT diversity, thereby limiting our predictions for their evolutionary origin and function. In order to better understand the diversity of NILTs and their possible roles in immune function, we identified five NILT loci in the Atlantic salmon (Salmo salar) genome, defined 86 NILT Ig domains within a 3-Mbp region of zebrafish (Danio rerio) chromosome 1, and described 41 NILT Ig domains as part of an alternative haplotype for this same genomic region. We then identified transcripts encoded by 43 different NILT genes which reflect an unprecedented diversity of Ig domain sequences and combinations for a family of non-recombining receptors within a single species. Zebrafish NILTs include a sole putative activating receptor but extensive inhibitory and secreted forms as well as membrane-bound forms with no known signaling motifs. These results reveal a higher level of genetic complexity, interindividual variation, and sequence diversity for NILTs than previously described, suggesting that this gene family likely plays multiple roles in host immunity.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":"75 1","pages":"53-69"},"PeriodicalIF":2.9,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10756952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling. 欧洲苦鱼主要MHC-DAB1等位基因谱系的个体拷贝数变异和广泛多样性。

IF 3.2 4区医学

Immunogenetics Pub Date : 2022-10-01 Epub Date: 2022-01-11 DOI: 10.1007/s00251-021-01251-4

Lorenzo Talarico, Anna Bryjová, Dagmar Čížková, Karel Douda, Martin Reichard

{"title":"Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling.","authors":"Lorenzo Talarico, Anna Bryjová, Dagmar Čížková, Karel Douda, Martin Reichard","doi":"10.1007/s00251-021-01251-4","DOIUrl":"https://doi.org/10.1007/s00251-021-01251-4","url":null,"abstract":"Polymorphism of the major histocompatibility complex (MHC), DAB1 gene was characterized for the first time in the European bitterling (Rhodeus amarus), a freshwater fish employed in studies of host-parasite coevolution and mate choice, taking advantage of newly designed primers coupled with high-throughput amplicon sequencing. Across 221 genotyped individuals, we detected 1-4 variants per fish, with 28% individuals possessing 3-4 variants. We identified 36 DAB1 variants, and they showed high sequence diversity mostly located within predicted antigen-binding sites, and both global and codon-specific excess of non-synonymous mutations. Despite deep divergence between two major allelic lineages, functional diversity was surprisingly low (3 supertypes). Overall, these findings suggest the role of positive and balancing selection in promotion and long-time maintenance of DAB1 polymorphism. Further investigations will clarify the role of pathogen-mediated selection to drive the evolution of DAB1 variation.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":"74 5","pages":"497-505"},"PeriodicalIF":3.2,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39686604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sex differences in immune gene expression in the brain of a small shorebird. 小滨鸟大脑中免疫基因表达的性别差异。

IF 3.2 4区医学

Immunogenetics Pub Date : 2022-10-01 Epub Date: 2022-01-27 DOI: 10.1007/s00251-022-01253-w

José O Valdebenito, Kathryn H Maher, Gergely Zachár, Qin Huang, Zhengwang Zhang, Larry J Young, Tamás Székely, Pinjia Que, Yang Liu, Araxi O Urrutia

{"title":"Sex differences in immune gene expression in the brain of a small shorebird.","authors":"José O Valdebenito, Kathryn H Maher, Gergely Zachár, Qin Huang, Zhengwang Zhang, Larry J Young, Tamás Székely, Pinjia Que, Yang Liu, Araxi O Urrutia","doi":"10.1007/s00251-022-01253-w","DOIUrl":"https://doi.org/10.1007/s00251-022-01253-w","url":null,"abstract":"Males and females often exhibit differences in behaviour, life histories, and ecology, many of which are typically reflected in their brains. Neuronal protection and maintenance include complex processes led by the microglia, which also interacts with metabolites such as hormones or immune components. Despite increasing interest in sex-specific brain function in laboratory animals, the significance of sex-specific immune activation in the brain of wild animals along with the variables that could affect it is widely lacking. Here, we use the Kentish plover (Charadrius alexandrinus) to study sex differences in expression of immune genes in the brain of adult males and females, in two wild populations breeding in contrasting habitats: a coastal sea-level population and a high-altitude inland population in China. Our analysis yielded 379 genes associated with immune function. We show a significant male-biased immune gene upregulation. Immune gene expression in the brain did not differ in upregulation between the coastal and inland populations. We discuss the role of dosage compensation in our findings and their evolutionary significance mediated by sex-specific survival and neuronal deterioration. Similar expression profiles in the coastal and inland populations suggest comparable genetic control by the microglia and possible similarities in pathogen pressures between habitats. We call for further studies on gene expression of males and females in wild population to understand the implications of immune function for life-histories and demography in natural systems.","PeriodicalId":13446,"journal":{"name":"Immunogenetics","volume":"74 5","pages":"487-496"},"PeriodicalIF":3.2,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792134/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39964069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

COVID-19: impact on Public Health and hypothesis-driven investigations on genetic susceptibility and severity. 新冠肺炎：对公共卫生的影响以及对遗传易感性和严重程度的假设驱动调查。

IF 2.9 4区医学

Immunogenetics Pub Date : 2022-08-01 Epub Date: 2022-03-29 DOI: 10.1007/s00251-022-01261-w

Susana David, Guillermo Dorado, Elsa L Duarte, Stephanie David-Bosne, João Trigueiro-Louro, Helena Rebelo-de-Andrade

引用次数: 0