Immunologic Research最新文献

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T cell receptor clonotype in tumor microenvironment contributes to intratumoral signaling network in patients with colorectal cancer. 肿瘤微环境中的 T 细胞受体克隆型与结直肠癌患者的瘤内信号网络有关。
IF 3.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI: 10.1007/s12026-024-09478-5
In Hye Song, Seung-Been Lee, Byung-Kwan Jeong, Jungwook Park, Honggeun Kim, GunHee Lee, Su Min Cha, Heejae Lee, Gyungyub Gong, Nak-Jung Kwon, Hee Jin Lee
{"title":"T cell receptor clonotype in tumor microenvironment contributes to intratumoral signaling network in patients with colorectal cancer.","authors":"In Hye Song, Seung-Been Lee, Byung-Kwan Jeong, Jungwook Park, Honggeun Kim, GunHee Lee, Su Min Cha, Heejae Lee, Gyungyub Gong, Nak-Jung Kwon, Hee Jin Lee","doi":"10.1007/s12026-024-09478-5","DOIUrl":"10.1007/s12026-024-09478-5","url":null,"abstract":"<p><p>Single-cell RNA sequencing (scRNA-seq) has contributed to understanding cellular heterogeneity and immune profiling in cancer. The aim of the study was to investigate gene expression and immune profiling in colorectal cancer (CRC) using scRNA-seq. We analyzed single-cell gene expression and T cell receptor (TCR) sequences in 30 pairs of CRC and matched normal tissue. Intratumoral lymphocytes were measured with digital image analysis. CRC had more T cells, epithelial cells, and myeloid cells than normal colorectal tissue. CRCs with microsatellite instability had more abundant T cells than those without microsatellite instability. Immune cell compositions of CRC and normal colorectal tissue were inversely correlated. CD4 + or CD8 + proliferating T cells, CD4 + effector memory T cells, CD8 + naïve T cells, and regulatory T cells of CRC showed higher TCR clonal expansion. Tumor epithelial cells interacted with immune cells more strongly than normal. T cells, myeloid cells, and fibroblasts from CRCs of expanded T cell clonotypes showed increased expression of genes related to TNF and NFKB signaling and T cell activation. CRCs of expanded T cell clonotypes also showed stronger cellular interactions among immune cells, fibroblasts, and endothelial cells. Pro-inflammatory CXCL and TNF signaling were activated in CRCs of expanded T cell clonotype. In conclusion, scRNA-seq analysis revealed different immune cell compositions, differential gene expression, and diverse TCR clonotype dynamics in CRC. TCR clonality expansion is associated with immune activation through T cell signaling and chemokine signaling. Patients with CRCs of expanded clonotype can be promising candidates for immunotherapy.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"921-937"},"PeriodicalIF":3.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SPDYC serves as a prognostic biomarker related to lipid metabolism and the immune microenvironment in breast cancer. SPDYC 是一种与乳腺癌脂质代谢和免疫微环境有关的预后生物标志物。
IF 3.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-06-18 DOI: 10.1007/s12026-024-09505-5
Xinxin Chen, Haojie Peng, Zhentao Zhang, Changnian Yang, Yingqi Liu, Yanzhen Chen, Fei Yu, Shanshan Wu, Lixue Cao
{"title":"SPDYC serves as a prognostic biomarker related to lipid metabolism and the immune microenvironment in breast cancer.","authors":"Xinxin Chen, Haojie Peng, Zhentao Zhang, Changnian Yang, Yingqi Liu, Yanzhen Chen, Fei Yu, Shanshan Wu, Lixue Cao","doi":"10.1007/s12026-024-09505-5","DOIUrl":"10.1007/s12026-024-09505-5","url":null,"abstract":"<p><p>Breast cancer remains the most common malignant carcinoma among women globally and is resistant to several therapeutic agents. There is a need for novel targets to improve the prognosis of patients with breast cancer. Bioinformatics analyses were conducted to explore potentially relevant prognostic genes in breast cancer using The Cancer Genome Atlas (TCGA) and The Gene Expression Omnibus (GEO) databases. Gene subtypes were categorized by machine learning algorithms. The machine learning-related breast cancer (MLBC) score was evaluated through principal component analysis (PCA) of clinical patients' pathological statuses and subtypes. Immune cell infiltration was analyzed using the xCell and CIBERSORT algorithms. Kyoto Encyclopedia of Genes and Genomes enrichment analysis elucidated regulatory pathways related to speedy/RINGO cell cycle regulator family member C (SPDYC) in breast cancer. The biological functions and lipid metabolic status of breast cancer cell lines were validated via quantitative real-time polymerase chain reaction (RT‒qPCR) assays, western blotting, CCK-8 assays, PI‒Annexin V fluorescence staining, transwell assays, wound healing assays, and Oil Red O staining. Key differentially expressed genes (DEGs) in breast cancer from the TCGA and GEO databases were screened and utilized to establish the MLBC score. Moreover, the MLBC score we established was negatively correlated with poor prognosis in breast cancer patients. Furthermore, the impacts of SPDYC on the tumor immune microenvironment and lipid metabolism in breast cancer were revealed and validated. SPDYC is closely related to activated dendritic cells and macrophages and is simultaneously correlated with the immune checkpoints CD47, cytotoxic T lymphocyte antigen-4 (CTLA-4), and poliovirus receptor (PVR). SPDYC strongly correlated with C-C motif chemokine ligand 7 (CCL7), a chemokine that influences breast cancer patient prognosis. A significant relationship was discovered between key genes involved in lipid metabolism and SPDYC, such as ELOVL fatty acid elongase 2 (ELOVL2), malic enzyme 1 (ME1), and squalene epoxidase (SQLE). Potent inhibitors targeting SPDYC in breast cancer were also discovered, including JNK inhibitor VIII, AICAR, and JW-7-52-1. Downregulation of SPDYC expression in vitro decreased proliferation, increased the apoptotic rate, decreased migration, and reduced lipid droplets. SPDYC possibly influences the tumor immune microenvironment and regulates lipid metabolism in breast cancer. Hence, this study identified SPDYC as a pivotal biomarker for developing therapeutic strategies for breast cancer.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"1030-1050"},"PeriodicalIF":3.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring CCR5 + T regulatory cell subset dysfunction in type 1 diabetes patients: implications for immune regulation. 探索 1 型糖尿病患者 CCR5 + T 调节细胞亚群的功能障碍:对免疫调节的影响。
IF 4.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-06-27 DOI: 10.1007/s12026-024-09508-2
Ławrynowicz Urszula, Juhas Ulana, Słomiński Bartosz, Okońska Maja, Myśliwiec Małgorzata, Ryba-Stanisławowska Monika
{"title":"Exploring CCR5 + T regulatory cell subset dysfunction in type 1 diabetes patients: implications for immune regulation.","authors":"Ławrynowicz Urszula, Juhas Ulana, Słomiński Bartosz, Okońska Maja, Myśliwiec Małgorzata, Ryba-Stanisławowska Monika","doi":"10.1007/s12026-024-09508-2","DOIUrl":"10.1007/s12026-024-09508-2","url":null,"abstract":"<p><p>T regulatory lymphocytes (Treg) expressing CCR5 exhibit strong suppression activity in various autoimmune disorders. However, there remains a lack of comprehensive understanding regarding their involvement in the development of type 1 diabetes (T1D). In this study, we examined the role of the CCR5/CCL5 axis in regulating inflammatory response and its impact on regulatory T cells in type 1 diabetes (T1D). We hypothesize that dysregulation of the CCR5/CCL5 axis contributes to the development and progression of T1D through modulation of Treg-dependent immune responses. We analyzed the expression levels of CCR5 on Tregs isolated from individuals with T1D, as well as the plasma concentration of its main ligands. We found that Tregs from T1D patients exhibited decreased expression of CCR5 compared to healthy controls. Additionally, we observed a correlation between the expression levels of CCR5 on Tregs and their immunosuppressive function in T1D patients. Our results indicate the impaired migratory capacity of CCR5 + Tregs, suggesting a possible link between the dysregulation of the CCR5/CCL5 axis and impaired immune regulation in T1D. In line with previous studies, our findings support the notion that dysregulation of the CCR5/CCL5 axis contributes to the development and progression of type 1 diabetes (T1D) by modulating Treg-dependent immune responses. The decreased expression of CCR5 on Tregs in T1D patients suggests a potential impairment in the migratory capacity of these cells, which could compromise their ability to suppress autoreactive T cells and maintain immune homeostasis. Furthermore, our study highlights the importance of CCR5 as a biomarker for identifying dysfunctional Tregs in T1D.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"1061-1070"},"PeriodicalIF":4.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11564404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141467705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regulation of Tert methylation alleviates food allergy via regulating the Tert-IL10 signal pathway. 通过调节 Tert-IL10 信号通路来调节 Tert 甲基化,从而缓解食物过敏。
IF 3.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-07-22 DOI: 10.1007/s12026-024-09504-6
Haotao Zeng, Lingzhi Xu, Jiangqi Liu, Lihua Mo, Minyao Li, Shuo Song, Xuejie Xu, Shihan Miao, Miao Zhao, Pingchang Yang
{"title":"Regulation of Tert methylation alleviates food allergy via regulating the Tert-IL10 signal pathway.","authors":"Haotao Zeng, Lingzhi Xu, Jiangqi Liu, Lihua Mo, Minyao Li, Shuo Song, Xuejie Xu, Shihan Miao, Miao Zhao, Pingchang Yang","doi":"10.1007/s12026-024-09504-6","DOIUrl":"10.1007/s12026-024-09504-6","url":null,"abstract":"<p><strong>Background: </strong>The cause of food allergy (FA) is still a mystery. Telomerases are involved in the regulation of immune responses. This study aims to gain an understanding of the contribution of telomerase reverse transcriptase (TERT) to the pathogenesis of FA.</p><p><strong>Methods: </strong>A murine FA model was established with ovalbumin as the specific antigen. The role of TERT in regulating dendritic cell (DC) immune tolerogenic functions was evaluated in this murine model.</p><p><strong>Results: </strong>We observed that the Tert promoter was at demethylation status and the Tert expression was elevated in DCs of FA mice. The Tert expression in DCs had a positive correlation with the FA response. TERT prevented the induction of Il10 expression in DCs. The immune tolerogenic functions of DCs were diminished by TERT. The immune tolerogenic functions of DC were restored by CpG by boosting the Tert promoter methylation. Administration of CpG promoted the therapeutic effects of allergen specific immunotherapy in FA mice.</p><p><strong>Conclusions: </strong>Low levels of Il10 expression and high levels of Tert expression were observed in intestinal DCs of FA mice. CpG exposure restored the expression of Il10 and increased the therapeutic benefits of allergen-specific immunotherapy.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"1018-1029"},"PeriodicalIF":3.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endogenous innate sensor NLRP3 is a key component in peritoneal macrophage dynamics required for cestode establishment. 内源性先天性传感器 NLRP3 是腹腔巨噬细胞动态的关键组成部分,是绦虫建立所必需的。
IF 4.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-06-06 DOI: 10.1007/s12026-024-09496-3
Irán Flores-Sotelo, Natalia Juárez, Marisol I González, Auraamellaly Chávez, Danielle T Vannan, Bertus Eksteen, Luis I Terrazas, José L Reyes
{"title":"Endogenous innate sensor NLRP3 is a key component in peritoneal macrophage dynamics required for cestode establishment.","authors":"Irán Flores-Sotelo, Natalia Juárez, Marisol I González, Auraamellaly Chávez, Danielle T Vannan, Bertus Eksteen, Luis I Terrazas, José L Reyes","doi":"10.1007/s12026-024-09496-3","DOIUrl":"10.1007/s12026-024-09496-3","url":null,"abstract":"<p><p>The NLRP3 receptor can assemble inflammasome platforms to trigger inflammatory responses; however, accumulating evidence suggests that it can also display anti-inflammatory properties. Here, we explored the role of nucleotide-binding oligomerization domain pyrin-containing protein 3 (NLRP3) in Taenia crassiceps experimental infection, which requires immune polarization into a Th2-type profile and peritoneal influx of suppressive macrophages for successful colonization. NLRP3 deficient mice (NLRP3<sup>-/-</sup>) were highly resistant against T. crassiceps, relative to wild-type (WT) mice. Resistance in NLRP3<sup>-/-</sup> mice was associated with a diminished IL-4 output, high levels of IL-15, growth factor for both innate and adaptive lymphocytes, and a dramatic decrease in peritoneum-infiltrating suppressive macrophages. Also, a transcriptional analysis on bone marrow-derived macrophages exposed to Taenia-secreted antigens and IL-4 revealed that NLRP3<sup>-/-</sup> macrophages express reduced transcripts of relm-α and PD-1 ligands, markers of alternative activation and suppressive ability, respectively. Finally, we found that the resistance displayed by NLRP3<sup>-/-</sup> mice is transferred through intestinal microbiota exchange, since WT mice co-housed with NLRP3<sup>-/-</sup> mice were significantly more resistant than WT animals preserving their native microbiota. Altogether, these data demonstrate that NLRP3 is a component of innate immunity required for T. crassiceps to establish, most likely contributing to macrophage recruitment, and controlling lymphocyte-stimulating cytokines such as IL-15.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"948-963"},"PeriodicalIF":4.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11564225/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141261778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predictive biomarkers of response to tocilizumab in giant cell arteritis (GCA): correlations with imaging activity. 巨细胞动脉炎(GCA)患者对西利珠单抗反应的预测性生物标志物:与成像活动的相关性。
IF 3.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-08-30 DOI: 10.1007/s12026-024-09518-0
Maurizio Benucci, Ilaria Di Girolamo, Antonino Di Girolamo, Francesca Li Gobbi, Arianna Damiani, Serena Guiducci, Barbara Lari, Valentina Grossi, Maria Infantino, Mariangela Manfredi
{"title":"Predictive biomarkers of response to tocilizumab in giant cell arteritis (GCA): correlations with imaging activity.","authors":"Maurizio Benucci, Ilaria Di Girolamo, Antonino Di Girolamo, Francesca Li Gobbi, Arianna Damiani, Serena Guiducci, Barbara Lari, Valentina Grossi, Maria Infantino, Mariangela Manfredi","doi":"10.1007/s12026-024-09518-0","DOIUrl":"10.1007/s12026-024-09518-0","url":null,"abstract":"<p><p>In the recent EULAR recommendations, ultrasound examination is now recommended as a first-line imaging test in all patients with suspected giant cell arteritis (GCA) and the axillary arteries should be included in the standard exam. As an alternative to ultrasound evaluation, cranial and extracranial arteries can be examined using FDG-PET or MRI. The aim of our study was to observe in a retrospective case series whether there is a correlation between biomarkers and imaging activity in a population of patients followed in real life with GCA treated with prednisone (PDN) and tocilizumab (TCZ). We retrospectively enrolled 68 patients with newly diagnosed GCA between January 2020 and September 2021, followed in real life, who were examined at the Rheumatology Unit of the San Giovanni di Dio Hospital, Florence, Italy. Patients were evaluated at T0-T3-T6-T12-T18-T24 for the following blood tests: ESR, CRP, fibrinogen, platelet count, serum amyloid A (SAA), IL-6, and circulating calprotectin (MRP). Ultrasound examination of the temporal arteries and axillary arteries was assessed at T0 within 7 days of starting treatment with high-dose glucocorticoids and subsequently at T3-T6-T12-T18-T24. A scale from 0 to 3 with semi-quantitative tools (SUV max) was assessed at T0-T12-T24. The evaluation of the correlation coefficient between laboratory and imaging variables has shown that SAA and MRP have the most powerful correlation with the PET score (0.523 and 0.64), and MRP also has an excellent correlation coefficient with the Halo score (0.658). The evaluation of the ROC curves shows for a PET score 3 and SAA values higher than 26 mg/L, sensitivity of 81.5% and specificity of 84.1%, and for a PET score 3 and MRP values higher than 2.3 mcg/mL, sensitivity of 100% and specificity of 76.8%. In this study, we demonstrated that SAA and MRP can be useful as promising tools to detect GCA activity. The study demonstrates a good correlation between the two biomarkers and the imaging activity evaluated by the Halo and PET scores.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"1154-1160"},"PeriodicalIF":3.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of NLRP3 inflammasome activation in patients with chronic obstructive pulmonary disease before and after lung transplantation. 评估肺移植前后慢性阻塞性肺病患者体内 NLRP3 炎症小体的激活情况。
IF 4.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-05-29 DOI: 10.1007/s12026-024-09497-2
Lada Rumora, Ivona Markelić, Iva Hlapčić, Andrea Hulina Tomašković, Marija Fabijanec, Feđa Džubur, Miroslav Samaržija, Andrea Vukić Dugac
{"title":"Assessment of NLRP3 inflammasome activation in patients with chronic obstructive pulmonary disease before and after lung transplantation.","authors":"Lada Rumora, Ivona Markelić, Iva Hlapčić, Andrea Hulina Tomašković, Marija Fabijanec, Feđa Džubur, Miroslav Samaržija, Andrea Vukić Dugac","doi":"10.1007/s12026-024-09497-2","DOIUrl":"10.1007/s12026-024-09497-2","url":null,"abstract":"<p><p>The interplay between purinergic receptors as well as pattern recognition receptors like Toll-like receptors (TLRs) and NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) might have a role in the pathogenesis of chronic obstructive pulmonary disease (COPD). The aim of this study was to determine and compare the concentrations of the damage-associated molecular patterns (DAMPs) heat shock protein 70 (Hsp70) and adenosine triphosphate (ATP), and gene expression of their respective receptors as well as NLRP3 inflammasome-related molecules in the peripheral blood of patients with end-stage COPD before and 1 year after lung transplantation (LT). Lung function was assessed by spirometry and diffusion capacity for carbon monoxide (DLCO). Quantitative polymerase chain reaction (qPCR) was applied for detection of TLR2, TLR4, P2X7R, P2Y2R, IL1B, CASP1, and NLRP3 expression. High-sensitivity ELISA kits were used for extracellular (e) Hsp70 and IL-1β, and luminescence assay for eATP measurements. Concentrations of eHsp70 and eATP as well as IL-1β were significantly increased in the plasma of end-stage COPD patients and significantly decreased after LT. In addition, TLR4, P2Y2R, IL1B, CASP1, and NLRP3 expression was up-regulated in COPD patients before LT, while it was significantly suppressed after LT. In conclusion, it could be assumed that NLRP3 inflammasome is activated in the peripheral blood of end-stage COPD patients and that eHsp70 and eATP could be responsible for its activation through triggering their receptors. On the other hand, previously enhanced pro-inflammatory reactions seem to be suppressed to the healthy population levels in lung recipients without allograft rejection.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"964-974"},"PeriodicalIF":4.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11564204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of the relationship of tissue-resident γδ T cells and IL-17 gene expression with the pathogenesis of autoimmune hepatitis. 组织驻留γδT细胞和IL-17基因表达与自身免疫性肝炎发病机制关系的研究
IF 3.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-07-18 DOI: 10.1007/s12026-024-09515-3
Nurullah Yucel, Gulam Hekimoglu, Sevinc Keser, Selma Erhan, Gamze Yesilay, Gulizar Hocaoglu, Muzaffer Seker
{"title":"Investigation of the relationship of tissue-resident γδ T cells and IL-17 gene expression with the pathogenesis of autoimmune hepatitis.","authors":"Nurullah Yucel, Gulam Hekimoglu, Sevinc Keser, Selma Erhan, Gamze Yesilay, Gulizar Hocaoglu, Muzaffer Seker","doi":"10.1007/s12026-024-09515-3","DOIUrl":"10.1007/s12026-024-09515-3","url":null,"abstract":"<p><p>Autoimmune hepatitis (AIH) is a chronic inflammatory liver disease. Elevated serum immunoglobulin G (IgG) levels, autoantibodies, and histopathological interface hepatitis are the hallmarks of AIH. Autoantibodies and pathological findings, clinical and biochemical features, typical immunoglobulin levels, and exclusion of other diseases are used to diagnose the condition. Gamma-delta (γδ) T cells are a unique population of unconventional T cells with γ and δ glycoprotein chains. γδ T cells have been shown to play a crucial role in autoimmune diseases by producing interleukin (IL)-17. However, its role in AIH remains to be further elucidated. In this study, we aimed to examine the role of γδ T cells and IL-17 in the pathogenesis of AIH, by working on biopsy samples. Paraffin blocks of 18 patients with type 1 AIH and 18 control liver tissues were analyzed. qRT-PCR assessed IL-17 gene expression. Immunofluorescence double staining of CD3<sup>+</sup>TCRγδ<sup>+</sup> was performed to reveal tissue-resident γδ T cells' role in AIH. When comparing AIH to the control, there was a substantial increase in the ratio of CD3<sup>+</sup>TCRγδ<sup>+</sup> cells in total inflammatory cells (p = 0.01). IL-17 gene expression was lowered in AIH when compared to the control (p = 0.01). This study provides evidence for the involvement of γδ T cells and IL-17 in the pathogenesis of AIH. The ratio of γδ T cells and IL-17 gene expression showed a significant difference in AIH suggesting a potential role for γδ T cells in driving liver inflammation in A fIH.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"895-901"},"PeriodicalIF":3.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Elevated total serum IgM predicts the presence of antiphospholipid antibodies in dysautonomia patients. 血清总 IgM 升高可预测自律神经失调症患者体内是否存在抗磷脂抗体。
IF 3.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-08-12 DOI: 10.1007/s12026-024-09510-8
Jill R Schofield, Jill Brook, Denise Calaprice-Whitty
{"title":"Elevated total serum IgM predicts the presence of antiphospholipid antibodies in dysautonomia patients.","authors":"Jill R Schofield, Jill Brook, Denise Calaprice-Whitty","doi":"10.1007/s12026-024-09510-8","DOIUrl":"10.1007/s12026-024-09510-8","url":null,"abstract":"<p><p>Dysautonomia is an abnormal clinical state with multiple etiologies, including autoimmunity. Antiphospholipid antibodies (aPL) are among the autoantibodies that have been associated with autonomic dysfunction. We have observed that an elevated total serum IgM appears to be associated with the presence of aPL in dysautonomia patients. This is a retrospective study analyzing the clinical characteristics of 45 consecutive patients with cardiac autonomic dysfunction and a persistently elevated total serum IgM. 93% of patients were female with a mean age of 32.7 years. Most patients had severely disabling disease, with a mean Karnofsky-like functional ability score of 42% (normal 100%). 93% of patients tested persistently positive for one or more aPL and all patients tested persistently positive for aPL and/or Sjogren's antibodies. No patient had lupus specific antibodies. One third of patients experienced one or more thrombotic events and 58% of patients attempting pregnancy experienced pregnancy morbidity. Lastly, 78% of aPL-positive patients treated with antithrombotic therapy experienced 50 to 100% improvement in one or more symptoms (e.g., migraine, cognitive dysfunction) recognized to be responsive to antithrombotic therapy in a subset of aPL-positive patients and 73% of patients treated with and tolerating immune modulatory therapy experienced a positive response. We propose total serum IgM as a reliable and inexpensive test that can be used to identify dysautonomia patients at risk for persistent aPL-positivity. These patients are important to identify as they have a significant risk for thrombosis and pregnancy morbidity and often experience significant symptomatic improvement with antithrombotic therapy and/or immune modulatory therapy.</p>","PeriodicalId":13389,"journal":{"name":"Immunologic Research","volume":" ","pages":"1086-1091"},"PeriodicalIF":3.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The prognostic value of IgA anti-citrullinated protein antibodies and rheumatoid factor in an early arthritis population with a treat-to-target approach. 在早期关节炎人群中,IgA 抗瓜氨酸蛋白抗体和类风湿因子的预后价值。
IF 3.3 4区 医学
Immunologic Research Pub Date : 2024-10-01 Epub Date: 2024-07-03 DOI: 10.1007/s12026-024-09500-w
Judith W Heutz, Agnes E M Looijen, Jac H S A M Kuijpers, Marco W J Schreurs, Annette H M van der Helm-van Mil, Pascal H P de Jong
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