O. P. Yadav, A. Razdan, B. Kumar, P. Singh, A. Singh
{"title":"Using AMMI approach to delineate genotype by environment interaction and stability of barley (Hordeum vulgare L.) genotypes under northern Indian shivalik hill conditions","authors":"O. P. Yadav, A. Razdan, B. Kumar, P. Singh, A. Singh","doi":"10.31742/IJGPB.80.3.15","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.15","url":null,"abstract":"Genotype by environment interaction (GEI) of 18 barley varieties was assessed during two successive rabi crop seasons so as to identify high yielding and stable barley varieties. AMMI analysis showed that genotypes (G), environment (E) and GEI accounted for 1672.35, 78.25 and 20.51 of total variance, respectively. Partitioning of sum of squares due to GEI revealed significance of interaction principal component axis IPCA1 only On the basis of AMMI biplot analysis DWRB 137 (41.03qha–1), RD 2715 (32.54qha–1), BH 902 (37.53qha–1) and RD 2907 (33.29qha–1) exhibited grain yield superiority of 64.45, 30.42, 50.42 and 33.42 per cent, respectively over farmers’ recycled variety (24.43qha–1).","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80799761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Singh, Monika Dubey, Neeraj Gurjar, Balbeer, Priyamedha, M. Meena, P. Sharma, P. Rai
{"title":"Genetics of white rust resistance in Indian mustard (Brassica juncea L.) and its validation on using molecular markers","authors":"V. Singh, Monika Dubey, Neeraj Gurjar, Balbeer, Priyamedha, M. Meena, P. Sharma, P. Rai","doi":"10.31742/IJGPB.80.3.6","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.6","url":null,"abstract":"White rust resistance loci (AcB1-A4.1 and AcB1-A5.1) associated with intron polymorphic (IP) markers i.e. At5g41560 and At2g36360, respectively, were used for validation of P1, P2, F1, F2, BC1F1 and BC2F1 generations. The donor parents namely, Bio-YSR and BEC-144 produced desired banding pattern of 430 and 750 bp while recipients viz., NRCHB 101 and DRMR-150-35 exhibited different pattern from donors confirming white rust resistance loci 4.1 and 5.1 with marker At5g41560 and At2g36360, respectively. Confirmation of these set of two IP markers in the parents and F1s lead us to further screening of selected F2, BC1F1 and BC2F1 populations. Available data on white rust reaction in different generations under study revealed that single dominant gene is responsible for white rust resistance. Potential of molecular markers in developing white rust resistant genotypes is proved under present study.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86166911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sanjeev Kumar, H. Choudhary, J. P. Sharma, Anil Kumar, Rubby Sandhu, Rucku Gupta, Vikas Gupta, A. Singh
{"title":"Study on genotype x environment interactions and AMMI analysis for agronomic traits in mungbean (Vigna radiata L. Wilczek.) under rainfed conditions.","authors":"Sanjeev Kumar, H. Choudhary, J. P. Sharma, Anil Kumar, Rubby Sandhu, Rucku Gupta, Vikas Gupta, A. Singh","doi":"10.31742/IJGPB.80.3.19","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.19","url":null,"abstract":"In the present study, 23 genotypes of mungbean were evaluated for stability performance under different environments continuously for three years during kharif 2016 (E1), 2017 (E2) and 2018 (E3) under rainfed conditions. Genotype Pusa Vishal exhibited regression coefficient equal to unity with non-significant deviation from regression coefficient and hence showed wider adaptability under poor or good environments. Genotype EC520016 showed earliness in maturity with wider adaptability. Environmental indices indicated that environment E2 and E3 were most favourable for yield and majority of yield attributing traits, whereas E3 alone was important for seed yield/plant and number of seeds/ pod. Based on AMMI models, Pusa Vishal, PD139, IPM2-3, IPM2057 and PML2-14 showed higher IPCA scores coupled with high population mean in E3. AMMI2 analysis indicated ML2056, and K851 with high IPCA1 in E3 whereas IPM99-125, BM63 and PM2-14 with high IPCA2 in the same environment. Genotypes which positioned very close to centre point and are least effected by G x E interactions while those presented away from the point of centre are more affected by G x E interactions and hence not stable. Identified genotypes may be utilized in improvement programme of Vigna radiata for targeted environments.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87451085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells","authors":"Zhang Yumiao, Wang Jun, Wu Tao","doi":"10.31742/IJGPB.80.3.17","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.17","url":null,"abstract":"In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45372138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of superior somaclones of aromatic local cultivar of rice (Oryza sativa L.)","authors":"B. Roy","doi":"10.31742/IJGPB.80.3.13","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.13","url":null,"abstract":"The traditional local aromatic rice cultivars are poor combiners. Here we report development of superior somaclones plants through tissue culture. The somaclones, TC 4/8 had the maximum yield followed by TC 5-1, TC 4/4, TC 4/5, and TC 4/7. Yield increase of TC 4/8, TC-5-1 was 54.75% and 50.33% more over the yield of parental cultivar, respectively. The plant tissue culture thus may be recommended to create genetic variability in rice as a trustworthy biotechnological tool.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90220583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Micronuclei assay as biomarker for ethyl methane sulphonate (EMS) genotoxicity in Eclipta alba (L.) Hassk.","authors":"Radha Mishra, G. Kumar","doi":"10.31742/IJGPB.80.3.11","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.11","url":null,"abstract":"The eukaryotic cells are complemented with a single nucleus. However, several cells show abnormal small nuclei known as micronuclei, with addition to the main nucleus. These anomalous small nuclei along with main nucleus which may be the result of exposure of cell to cytotoxic agents (Chemicals/physical mutagens). These mutagens also cause structural or numerical chromosomal aberrations. The treatment of EMS, a chemical mutagen, induces micronuclei formation and nuclear as well as chromosomal peculiarities in Eclipta alba. The effect of micronuclei is discussed in terms of cytotoxicity of genotoxins and several evidences of nuclear polymorphism illustrated due to the mutagenic effect of EMS. Pollen viability was also examined through pollen fertility. Micronuclei causes the genomic instability and elimination of genome which is effectively put to use in breeding programmes for the creation of aneuploids and also for haploid lines.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78322022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Khulbe, A. Pattanayak, L. Kant, G. Bisht, M. Pant, V. Pandey, Rohit Kapil, N. Mishra
{"title":"Doubled haploid production in maize under Sub-montane Himalayan conditions using R1-nj-based haploid inducer TAILP1","authors":"R. Khulbe, A. Pattanayak, L. Kant, G. Bisht, M. Pant, V. Pandey, Rohit Kapil, N. Mishra","doi":"10.31742/IJGPB.80.3.4","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.4","url":null,"abstract":"The use of in vivo haploid induction system makes the doubled haploid (DH) technology easier to adopt for the conventional maize breeders. However, despite having played an important role in the initial developmental phases of DH technology, Indian maize research has yet to harvest its benefits. Haploid Inducer Lines (HILs) developed by CIMMYT are being widely used in maize breeding programmes in many countries including India. There, however, is no published information on the efficiency of DH line production using CIMMYT HILs in Indian maize breeding programmes. In the present study, the efficiency of DH production using CIMMYT’s tropically adapted inducer line TAILP1 was investigated with eight source populations including two of sweet corn. The average haploid induction rate (HIR) of TAILP1 was 5.48% with a range of 2.01 to 10.03%. Efficiency of DH production ranged from 0.14 to 1.87% for different source populations with an average of 1.07%. The information generated will be useful for maize breeders intending to use DH technology for accelerated development of completely homozygous lines.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84466545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mangal, A. Srivastava, S. Mirajkar, Khushbu B. Singh, V. Solanki, B. Mandal, P. Kalia
{"title":"Differential expression profiling of defense related genes for Leaf Curl Virus (ChiLCV) in resistant and susceptible genotypes of Chiili","authors":"M. Mangal, A. Srivastava, S. Mirajkar, Khushbu B. Singh, V. Solanki, B. Mandal, P. Kalia","doi":"10.31742/IJGPB.80.3.10","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.10","url":null,"abstract":"The present investigation was undertaken to study the expression of eight defense related genes in leaf curl resistant line DLS-Sel-10 and susceptible line Phule Mukta after different days post inoculation (dpi) with viruliferous white flies to understand their role in resistance to chilli leaf curl virus (ChiLCV). The expression level of Ca PPO, Ca AsPer, Ca ATP/ADP and CaTopoII was observed to be higher in resistant genotype DLS-Sel-10 than the susceptible Phule Mukta at all the time points studied. Expression of CaNBS-LRR increased up to 12 dpi while that of Ca Thionin, and Ca SKP1 increased up to 24 dpi in the resistant line, thereafter it started declining. The CaSPI expression did not show any specific pattern in both the test plants. The heat map clustered all the genes under study into two major clusters based on their expression profiles, one comprising CaAsPer, Ca-Thionin, CaATP/ADP transporter, CaPPO and CaTopoII while other group comprised CaSKPI, Ca NBS and CaSPI. The challenge inoculation of the test genotypes also revealed that viral titre increased at a much slower rate in DLS-Sel-10 than Phule Mukta, suggesting thereby that DLS-Sel-10 is resisting the accumulation of ChiLCV and has a more active defense machinery than Phule Mukta.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84552337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Choi, P. Basnet, Hana Yoo, N. Roy, R. Ramekar, Kyong-Cheul Park
{"title":"Identification of novel haplotype of a cyst nematode resistance gene, GmSNAP18 in soybean [Glycine max (L.) Merr.]","authors":"I. Choi, P. Basnet, Hana Yoo, N. Roy, R. Ramekar, Kyong-Cheul Park","doi":"10.31742/IJGPB.80.3.5","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.5","url":null,"abstract":"Soybean cyst nematode (SCN) is one of the most damaging pest of soybean. Discovery and characterization of the genes involved in SCN resistance are important in soybean breeding. Soluble NSF attachment protein (SNAP) genes are related to SCN resistance in soybean. SNAP genes include five gene families, and 2 haplotypes of exons 6 and 9 of SNAP18 are considered resistant to the SCN. In present study the haplotypes of GmSNAP18 were surveyed and chacterized in a total of 60 diverse soybean genotypes including Korean cultivars, landraces, and wild-types. The target region of exons 6 and 9 in GmSNAP18 region was amplified and sequenced to examine nucleotide variation. Characterization of 5 haplotypes identified in present study for the GmSNAP18 gene revealed two haplotypes as resistant, 1 as susceptible and two as novel. A total of twelve genotypes showed resistant haplotypes, and 45 cultivars were found susceptible. Interestingly, the two novel haplotypes were present in 3 soybean lines. The information provided here about the haplotypic variation of GmSNAP18 gene can be further explored for soybean breeding to develop resistant varieties.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76003265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ramya Rathod, K. Suman, P. M. Babu, C. Neeraja, K. Eswari, D. S. Chary, L. V. Subbarao
{"title":"Genetic studies for yield and nutritional traits in unpolished rice RILs of MTU1010 x BR2655","authors":"Ramya Rathod, K. Suman, P. M. Babu, C. Neeraja, K. Eswari, D. S. Chary, L. V. Subbarao","doi":"10.31742/IJGPB.80.3.12","DOIUrl":"https://doi.org/10.31742/IJGPB.80.3.12","url":null,"abstract":"A study was carried out to access the genetic variability in a set of 190 recombinant inbred lines (RILs) of rice derived from MTU1010 and BR2655. Plant height and number of productive tillers per plant recorded high PCV and GCV during kharif 2016, whereas number of filled grains per panicle, grain yield per plant, grain zinc concentration and grain iron concentration during rabi 2016-17.The estimates of PCV were slightly higher than the corresponding GCV estimates for all the traits studied indicating that the characters were least influenced by the environment. High heritability coupled with high genetic advance as per cent of mean was noticed for traits like plant height, number of productive tillers, number of filled grains per panicle, 1000-grain weight, grain yield per plant, grain iron concentration and grain zinc concentration respectively during kharif 2016 and rabi 2016-17 indicating that traits are under additive genetic control, simple selection would likely be effective for further improvement of these traits in desirable direction. RILs J16, J144 and J146 have shown higher grain yield per plant with high iron and zinc concentrations.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89053510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}