微核法测定白月牙(Eclipta alba)甲烷磺酸乙酯(EMS)遗传毒性Hassk。

IF 1 4区生物学 Q3 PLANT SCIENCES

Indian Journal of Genetics and Plant Breeding Pub Date : 2020-09-22 DOI:10.31742/IJGPB.80.3.11

Radha Mishra, G. Kumar

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引用次数: 0

摘要

真核细胞只有一个细胞核。然而，除了主核外，一些细胞显示异常的小核，称为微核。这些异常的小核和主核可能是细胞暴露于细胞毒性物质(化学物质/物理诱变剂)的结果。这些诱变剂也会引起染色体结构或数量畸变。EMS(一种化学诱变剂)的处理诱导了白月食的微核形成以及核和染色体的特性。本文从基因毒素的细胞毒性角度讨论了微核的作用，并阐述了由于基因毒素的致突变作用而引起的核多态性的几个证据。通过花粉育性测定花粉活力。微核导致基因组的不稳定和基因组的消除，这有效地用于非整倍体和单倍体系的培育计划。

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Micronuclei assay as biomarker for ethyl methane sulphonate (EMS) genotoxicity in Eclipta alba (L.) Hassk.

The eukaryotic cells are complemented with a single nucleus. However, several cells show abnormal small nuclei known as micronuclei, with addition to the main nucleus. These anomalous small nuclei along with main nucleus which may be the result of exposure of cell to cytotoxic agents (Chemicals/physical mutagens). These mutagens also cause structural or numerical chromosomal aberrations. The treatment of EMS, a chemical mutagen, induces micronuclei formation and nuclear as well as chromosomal peculiarities in Eclipta alba. The effect of micronuclei is discussed in terms of cytotoxicity of genotoxins and several evidences of nuclear polymorphism illustrated due to the mutagenic effect of EMS. Pollen viability was also examined through pollen fertility. Micronuclei causes the genomic instability and elimination of genome which is effectively put to use in breeding programmes for the creation of aneuploids and also for haploid lines.

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.