农杆菌介导洋葱表皮细胞转化的高效瞬时检测系统

IF 1 4区生物学 Q3 PLANT SCIENCES

Indian Journal of Genetics and Plant Breeding Pub Date : 2020-09-22 DOI:10.31742/IJGPB.80.3.17

Zhang Yumiao, Wang Jun, Wu Tao

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引用次数: 1

摘要

本研究对农杆菌感染培养基、感染时间、洗涤剂和细胞密度进行了优化。以高粱为基础的侵染培养基(SbIM)，侵染时间为10 ~ 20 min，添加0.01%的Silwet L-77，在600 nm光密度(OD600)的条件下，洋葱表皮细胞支持农杆菌侵染的能力提高了50% ~ 90%。细胞周期蛋白依赖性激酶D-2 (CDKD-2)和细胞色素c型生物发生蛋白(CYCH)，蛋白-蛋白相互作用定位。优化后的程序是一种快速有效的检测蛋白质亚细胞定位和蛋白质-蛋白质相互作用的系统。

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An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.