印度芥菜(Brassica juncea L.)抗白锈病遗传及其分子标记验证

IF 1 4区生物学 Q3 PLANT SCIENCES

Indian Journal of Genetics and Plant Breeding Pub Date : 2020-09-22 DOI:10.31742/IJGPB.80.3.6

V. Singh, Monika Dubey, Neeraj Gurjar, Balbeer, Priyamedha, M. Meena, P. Sharma, P. Rai

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引用次数: 1

摘要

利用与内含子多态性(IP)标记At5g41560和At2g36360相关的抗白锈病基因座(AcB1-A4.1和AcB1-A5.1)对P1、P2、F1、F2、BC1F1和BC2F1代进行验证。供体亲本Bio-YSR和becc -144产生了430和750 bp的条带模式，而受体亲本NRCHB 101和DRMR-150-35的条带模式与供体不同，分别用标记At5g41560和At2g36360确定了抗白锈病位点4.1和5.1。在亲本和F1s中确认这两组IP标记，使我们进一步筛选选定的F2、BC1F1和BC2F1群体。不同代间白锈病反应的现有资料表明，单显性基因对白锈病抗性起主导作用。目前的研究证实了分子标记在开发抗白锈病基因型中的潜力。

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Genetics of white rust resistance in Indian mustard (Brassica juncea L.) and its validation on using molecular markers

White rust resistance loci (AcB1-A4.1 and AcB1-A5.1) associated with intron polymorphic (IP) markers i.e. At5g41560 and At2g36360, respectively, were used for validation of P1, P2, F1, F2, BC1F1 and BC2F1 generations. The donor parents namely, Bio-YSR and BEC-144 produced desired banding pattern of 430 and 750 bp while recipients viz., NRCHB 101 and DRMR-150-35 exhibited different pattern from donors confirming white rust resistance loci 4.1 and 5.1 with marker At5g41560 and At2g36360, respectively. Confirmation of these set of two IP markers in the parents and F1s lead us to further screening of selected F2, BC1F1 and BC2F1 populations. Available data on white rust reaction in different generations under study revealed that single dominant gene is responsible for white rust resistance. Potential of molecular markers in developing white rust resistant genotypes is proved under present study.

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.