Savitha B Naik, N Kiran Kumar, P Annie Swathisha, Biji Brigit, H S Preetham, Chella S Karumaran
{"title":"Effect of Andrographis paniculata Herbal Extract on Cytotoxicity, Proliferation and Osteogenic/Odontogenic Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study.","authors":"Savitha B Naik, N Kiran Kumar, P Annie Swathisha, Biji Brigit, H S Preetham, Chella S Karumaran","doi":"10.4103/ijdr.ijdr_907_22","DOIUrl":"10.4103/ijdr.ijdr_907_22","url":null,"abstract":"<p><strong>Background: </strong>Research has been conducted to assess the regenerative potential of dental pulp stem cells (DPSCs) following pretreatment of stem cells with certain molecules, bioactive compounds, plant extract and physical stimulation. Andrographis paniculata (AP) herbal extract with important medicinal properties is proven to have a preosteogenic effect on osteoblasts.</p><p><strong>Aim: </strong>This study aimed to determine the effect of various concentrations of AP extract on the cytotoxicity and osteogenic and odontogenic potential of DPSCs.</p><p><strong>Methods and material: </strong>Dental pulp stem cells were subjected to treatment with various concentrations of AP herbal extract (7 ug/ml, 5.2 ug/ml, 3.5 ug/ml, 1.7 ug/ml and 0.8 ug/ml), following which the cells were subjected to tests-3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) analysis for cytotoxicity and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) test for expression of genes (bone morphogenetic protein (BMP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP) and alkaline phosphatase (ALP)).</p><p><strong>Results: </strong>AP extract at concentration of 0.8 ug/ml-5.2 ug/ml had no cytotoxicity supporting cell growth. 3.5 ug/ml showed significant upregulation of genes on the third day.</p><p><strong>Conclusion: </strong>AP, a commonly occurring medicinal plant through its effect on DPSCs, could serve as an effective pretreatment modality for cell-based regenerative therapy and vital pulp therapy.</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":"196-200"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sk Aziz Ikbal, Zeba R Siddiqui, Siddhartha Dutta, Tasneem Fatima, Roopanshi Mehrotra, Surendra K Yadav
{"title":"Surgical Lip Repositioning in Search of Aesthetics - A Case Series.","authors":"Sk Aziz Ikbal, Zeba R Siddiqui, Siddhartha Dutta, Tasneem Fatima, Roopanshi Mehrotra, Surendra K Yadav","doi":"10.4103/ijdr.ijdr_835_23","DOIUrl":"10.4103/ijdr.ijdr_835_23","url":null,"abstract":"<p><strong>Introduction: </strong>The gummy smile (GS) or excessive gum visibility (EGV) is an aesthetic concern that affects an individual's attractiveness and personality.</p><p><strong>Treatment planning: </strong>Lip Repositioning surgery (LRP) is a less invasive surgery that can be attributed to treating EGV due to hypermobile lip muscles or mild-to-moderate vertical maxillary excess (VME). Three patients went through LRP surgery by stripping overlying mucosa from the buccal vestibule, followed by suturing the lip mucosa to the mucogingival junction (MGJ), creating shallow vestibule and restricted muscle pull and reducing gingival visibility (GV) during a smile.</p><p><strong>Followup: </strong>Three months of follow-up showed a satisfactory result with 3.67 ± 0.58 mm mean reduction in GV.</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":"239-241"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Periodontal Therapy on Rheumatoid Disease Activity in Patients on Anti-Rheumatoid Drugs - A Prospective Study.","authors":"Arunachalam Kaveri, Velayudham Rajendran, Tukaram Kshirsagar Jaishree, Krishnamoorthy Malathi, Mohanasundaram Kavitha","doi":"10.4103/ijdr.ijdr_535_23","DOIUrl":"10.4103/ijdr.ijdr_535_23","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this clinical trial rheumatoid arthritis-chronic periodontitis (RA-CP) is to assess the effect of non-surgical periodontal therapy in RA patients.</p><p><strong>Materials and methods: </strong>Sixty RA patients taking disease-modifying anti-rheumatoid drugs (DMARD) with CP were selected from the Institute of Rheumatology, Madras Medical College, randomised into the intervention group (IG) and control group (CG). IG received non-surgical periodontal therapy after the screening visit, but CG did not receive treatment until the 3 months study period. Periodontal parameters were recorded at baseline and after 3 months for both groups. Rheumatoid Disease activity (RD activity) was assessed using disease activity score (DAS28), inclusive of tender joints count (TJC), swollen joints count (SJC), visual analog scale (VAS), and erythrocyte sedimentation rate (ESR) for both groups at baseline and after 3 months.</p><p><strong>Results: </strong>At the end of 3 months, IG had significant reduction in RA parameters such as tender joints count (0.0005), swollen joints count (0.0005), ESR (0.003), VAS score (0.0005), and DAS28 values (0.0005) compared to CG. Periodontal parameters at the end of 3 months were also significantly reduced in IG than in CG.</p><p><strong>Conclusion: </strong>Non-surgical periodontal therapy might have reduced the burden of systemic inflammatory markers, thus reducing severity of RA in IG. Reduction in tenderness and swelling of joints in the upper arm might have enabled the patients in IG to perform better oral hygiene maintenance procedures.</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":"154-159"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anupama Aradya, P K Kiran, K N Raghavendra Swamy, Vidya G Doddawad, Narahari Ranganatha, Koduru Sravani
{"title":"Oral Risk Factors in Patients with Cancer Undergoing Chemotherapy - A Pilot Study.","authors":"Anupama Aradya, P K Kiran, K N Raghavendra Swamy, Vidya G Doddawad, Narahari Ranganatha, Koduru Sravani","doi":"10.4103/ijdr.ijdr_516_23","DOIUrl":"10.4103/ijdr.ijdr_516_23","url":null,"abstract":"<p><strong>Background: </strong>Treatment modalities for cancer including surgery, radiotherapy and chemotherapy, have some complications even in the oral cavity. The literature describes oral lesions that may arise as a result of chemotherapy. However, information regarding oral symptoms in advanced cancer patients is poor.</p><p><strong>Objective: </strong>To identify the oral manifestations like oral mucositis, dry mouth and loss of taste in patients undergoing chemotherapy treatment.</p><p><strong>Methods: </strong>We evaluated 60 patients affected by malignancy undergoing chemotherapy treatment. The clinical and pathological data such as age, gender, diagnosis of malignancy and types of treatments with anticancer chemotherapeutic drug treatment, were obtained. Patients were routinely evaluated for the presence of oral mucositis based on the Common Terminology Criteria for Adverse Events (CTCAE) v5.0 scale for adverse effects and graded. Other findings like dry mouth and loss of taste were recorded.</p><p><strong>Results: </strong>Of the 60 patients, 40 (66.6%) were male, and 20 (33.3%) females with a mean age of 53.7 years. Most patients about 63% and 83% were diagnosed with dry mouth and loss of taste, respectively and 71% of patients had mucositis. Chemotherapy drugs like Cyclophosphamide, carboplatin, nanoxel, paclitaxel, oxaliplatin, docetaxel and doxorubicin, were directly associated with oral mucositis.</p><p><strong>Conclusion: </strong>Patients treated with chemotherapy for cancer most often suffer from a multitude of intense and debilitating oral dysfunctions. Oral lesions found in patients undergoing chemotherapy were mucositis, dry mouth and loss of taste. These adverse effects and an appropriate symptomatic therapy need to be discussed with the patients. Chemotherapy has a significant but transient effect on the oral symptoms.</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":"126-130"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Evaluation of Success of NeoPutty Mineral Trioxide Aggregate ® with Formocresol as Pulpotomy Medicaments in Primary Molars: A Clinical Study.","authors":"Sonu Acharya, Deepa Gurunathan","doi":"10.4103/ijdr.ijdr_730_23","DOIUrl":"10.4103/ijdr.ijdr_730_23","url":null,"abstract":"<p><strong>Objective: </strong>This clinical study was aimed to investigate the clinical and radiographic outcomes of NeoPutty MTA ® (Nusmile), a novel mineral trioxide aggregate (MTA) pulpotomy material, in primary teeth of children aged 4-6 years.</p><p><strong>Methods: </strong>An in vivo study was done in children aged 4-6 years old. A total of 50 primary second molar teeth were included in the study, with 25 of them undergoing treatment with formocresol (FC) and 25 receiving treatment with NeoPutty MTA ® . The subjects were divided into two groups for treatment: the first group received a treatment involving a 1:5 dilution of Buckley's FC. The second group, however, underwent treatment with NeoPutty MTA ® . This study employs a meticulous approach to evaluate the impact of different treatments on a significant number of molar teeth, using FC and NeoPutty MTA ® as the respective treatment methods. The demographics of the subject pool are well-documented, comprising a balance of male and female participants. The technique employed for pulp removal and the subsequent management of bleeding are outlined, underscoring the thoroughness of the process.</p><p><strong>Results: </strong>At 12 months, the NeoPutty MTA ® pulpotomy group showed a significantly higher success rate clinically (96%) compared to the FC group (72%) ( P < 0.05), but radiographically although NeoPutty MTA ® was better (92%) than FC (82%), it was not statistically significant. NeoPutty ® -treated teeth exhibited no signs of pain or discomfort and demonstrated better pulp vitality compared to the FC group.</p><p><strong>Conclusion: </strong>NeoPutty MTA ® demonstrated superior clinical success rates and better radiographic outcomes compared to traditional FC pulpotomy.</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":"170-175"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cytotoxicity and Alkaline Phosphatase Activity of Curcumin, Aloin and MTA on Human Dental Pulp Cells.","authors":"Dilara Dinc, Mehmet F Seyhan, Oya Aktoren","doi":"10.4103/ijdr.ijdr_1184_21","DOIUrl":"10.4103/ijdr.ijdr_1184_21","url":null,"abstract":"<p><strong>Aim: </strong>The objective of this in-vitro study was to assess the cytotoxicity and alkaline phosphatase (ALP) activity of curcumin and aloin extracted from Curcuma longa and Aloe vera , and mineral trioxide aggregate (MTA) on human dental pulp stem cells.</p><p><strong>Methods: </strong>Human dental pulp stem cells (Lonza Group, Switzerland), curcumin (Sigma-Aldrich, USA), aloin (Sigma-Aldrich, USA), and ProRoot MTA (Dentsply, USA) were used in the study. 2.5-6.75-12.5-25-50 μg/ml of curcumin and aloin, 25%-50%-75%-100% of MTA were prepared; pulp cells unincubated with a material were assessed as controls. Cytotoxicity of all doses/concentrations of materials was analysed on days of 1, 2, 3, and 7 by WST-1 test. 2.5-6.75 μg/ml of curcumin and aloin, 25%-50% of MTA incubated with cells for 7-14 days were evaluated for ALP activity by ELISA test. Data was statistically analysed by One Way ANOVA, Tukey, and Sidak tests at GraphPad Prism 6.</p><p><strong>Results: </strong>The findings have shown that 2.5 μg/ml of curcumin, all doses of aloin, 25% and 50% of MTA increased cell proliferation significantly on day 1 ( P < 0.05). Curcumin, aloin, and MTA decreased the cell viability as dose/concentration and exposure time increased. All materials have shown no significant increases in ALP activity ( P > 0.05) on 7 and 14 days.</p><p><strong>Conclusion: </strong>Data of this study revealed that 2.5 - 6.75 μg/ml of curcumin/aloin, 25%-50% of MTA have promoted cell viability and proliferation of human dental pulp cells; and none of the materials have significantly increased the ALP activity at 7-14 days.</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":"216-220"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dental Research and Noncommunicable Diseases.","authors":"S M Balaji","doi":"10.4103/ijdr.ijdr_664_24","DOIUrl":"10.4103/ijdr.ijdr_664_24","url":null,"abstract":"","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":"35 2","pages":"125"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohit Awaradi, Roshan M Sagarkar, Prashantha G Shivamurthy, Silju Mathew, Sharanya Sabrish, Nirajita Bhaduri
{"title":"Evaluation of Stresses Generated with Two Different Self-Ligating Orthodontic Bracket Systems - A Finite Element Analysis.","authors":"Mohit Awaradi, Roshan M Sagarkar, Prashantha G Shivamurthy, Silju Mathew, Sharanya Sabrish, Nirajita Bhaduri","doi":"10.4103/ijdr.ijdr_937_23","DOIUrl":"10.4103/ijdr.ijdr_937_23","url":null,"abstract":"<p><strong>Aim: </strong>To determine stresses generated in the posterior teeth region by two different self-ligating systems, 3M Unitek SmartClip and Damon Q Self-ligating brackets, using the finite element method (FEM).</p><p><strong>Methodology: </strong>Computed tomography (CT) scan of maxilla was taken using Mimics software, and the Dicom data were imported into Hypermesh 13.0 software to convert it into a geometric model and then into FEM. Physical models of both the brackets were constructed and positioned on the teeth. Post archwire modeling, contact is defined between the bracket slot and 0.014\" NiTi arch wire. The final assembled model was exported to ANSYS 12.1 for analysis, and 60 grams of load was applied at the ends of the arch wire.</p><p><strong>Results: </strong>On using 0.014\" CuNiTi in both the bracket systems, stresses generated on the root surface were found to be maximum on the first molar region, followed by the first premolar, second molar, canine, and second premolar regions. Comparison between the two bracket systems showed differences in the stresses generated, where higher stresses were generated at all regions in the Damon Q bracket system, except the inter-second premolar region.</p><p><strong>Conclusion: </strong>Maximum stresses were observed with the 3M Unitek SmartClip bracket system at the second premolar cervical region when compared to the Damon Q system. In both the cortical bone and cancellous bone, maximum stresses were observed at the second premolar cervical in the Damon Q bracket system region.</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":"131-135"},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142286025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Evaluation of Implant-Protected Occlusion in Partially Edentulous Fixed Restoration Using Qualitative and Quantitative Assessment – A Prospective Clinical Trial","authors":"Ramesh Chowdhary, S. Bukkapatnam","doi":"10.4103/ijdr.ijdr_191_23","DOIUrl":"https://doi.org/10.4103/ijdr.ijdr_191_23","url":null,"abstract":"\u0000 \u0000 \u0000 To compare and evaluate the occlusal variability/discrepancy recorded using qualitative (articulating paper) and quantitative (T-scan) techniques in partially edentulous implant-supported restorations.\u0000 \u0000 \u0000 \u0000 A total of 20 patients in the age group of 25–61 yrs participated in this study. All the patients had more than one tooth replaced with implant-supported prosthesis. After three months of restoration, occlusion reevaluated was carried out using qualitative (articulating paper) and quantitative (T-scan). T-scan data were used to measure implant occlusion time, occlusion time, and the relative occlusal force (ROFs) on implant prosthesis. And the data were statistically evaluated.\u0000 \u0000 \u0000 \u0000 The T-scan values showed high points on the implants in most of the patients, which could not be evaluated by that of articulating paper outcomes.\u0000 \u0000 \u0000 \u0000 The occlusal harmony achieved from using qualitative analysis of articulating paper was not supported by the finding of the quantitative analysis (T-scan). Also the T-scan provided the time interval of occlusion of the implant-supported restorations, which would help in better defining the implant-protected occlusal.\u0000 \u0000 \u0000 \u0000 The T-scan occlusal analysis system provides ROF and time intervals of occlusal contact. This allows accurate occlusal equilibration of implant-supported restoration according to implant protected occlusal concept.\u0000","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":"52 40","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139961350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An In Vitro Comparative Study of the Push-out Bond Strengths of Various Endodontic Sealers to Radicular Dentin","authors":"Mohamed Abdelaziz El Sayed, Safiya Saeed Alderei","doi":"10.4103/ijdr.ijdr_708_22","DOIUrl":"https://doi.org/10.4103/ijdr.ijdr_708_22","url":null,"abstract":"\u0000 \u0000 \u0000 The bond strength of an endodontic sealer to the root dentin is an important factor in maintaining the integrity of the root canal seal. The present study aimed to assess the push-out bond strength of three endodontic sealers used with the single-cone obturation technique.\u0000 \u0000 \u0000 \u0000 The root canals of 40 central maxillary incisors were instrumented using ProTaper Universal NiTi rotary files. The roots were divided into 4 groups (N = 10) according to the type of root canal sealers: Group 1 (control group): AH Plus/Gutta-percha lateral condensation technique (LC); Group 2: AH Plus/Gutta-percha single-cone technique (SC); Group 3: GuttaFlow 2/SC; Group 4: Realseal SE/SC. After obturation, each tooth was prepared for push-out assessment. The total and sectional push-out bond strengths were measured and statistically analysed. Furthermore, failure modes were identified microscopically.\u0000 \u0000 \u0000 \u0000 The results indicated that samples in the first group showed the highest total and sectional bond strengths. Samples from other groups showed statistically similar total bond strengths. The highest sectional bond strength was found mainly in the apical root sections. Cohesive failure was predominant for the first group, whereas adhesive failure was predominant in the other groups.\u0000 \u0000 \u0000 \u0000 It could be concluded that the dentin bond strengths of the tested sealers used with the single-cone technique were nearly similar but lower than those of AH Plus/LC. The highest sectional bond strength was observed in the apical thirds. The failure mode was mostly cohesive for AH Plus/LC and adhesive for sealers used with the single-cone obturation technique.\u0000","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":"58 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139960933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}