Indian journal of experimental biology最新文献

{"title":"Molecular targets and mechanism of Longji Xiaozhong ointment in treating acute ankle sprain: A network pharmacology based analysis","authors":"Yumei Li, Qing Zhang, Haixia Zhu, De-ta Chen, Tian-you Fan","doi":"10.56042/ijeb.v61i12.6811","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.6811","url":null,"abstract":"Acute ankle sprain is one of the most common lower limb injuries particularly in athletes and it accounts for 16-40% of all sports-related injuries. A significant proportion of patients experience persistent residual symptoms and recurrence. Traditional Chinese medicine (TCM) offers effective treatment in terms of pain relief, swelling reduction, functional improvement, and shorter disease duration. This study provides a comprehensive analysis of the active ingredients, molecular targets, signaling pathways, and Longji Xiaozhong ointment's clinical efficacy for treating acute ankle sprains. We followed network pharmacology approach to identify the molecular targets and mechanism of action of Longji Xiaozhong ointment in treating acute ankle sprain and to validate its clinical value using relevant clinical data. Initially, Longji Xiaozhong Ointment's active ingredients were screened using the TCMSP platform based on drug properties similar to DLP ≥0.18 and oral bioavailability OB ≥30%. The molecular targets were identified from the ETCM database, followed by constructing protein-protein interaction (PPI) network diagrams and GO and pathway enrichment analysis on key genes. Furthermore, the UniProt database was used to investigate the 3D structures of these key genes. Finally, the clinical data of 111 patients with acute ankle sprains were retrospectively analyzed and further explored by comparing the different curative effects of Longji Xiaozhong ointment with Celebrex and Shexiang Analgesic ointment to check the clinical value of Longji Xiaozhong ointment in treating acute ankle sprain. In the TCMSP database, we found that there were 42 active ingredients in Longji Xiaozhong ointment, including 13 kinds of Strychnosnux-vomica L., 7 kinds of Ligusticum chuanxiong , and 22 kinds of Safflowers ( Carthamus tinctorius ), and a total of 3206 target genes were obtained. After applying the OB ≥50% criteriaand DL ≥0.5, 19 key target genes were selected based on their correspondence to the active ingredients. Protein mutual aid network construction and module analysis yielded two high-scoring Clusters and identified seven key proteins, including SLC6A4, ADRB2, ADRA1B, CHRM1, F2, OPRM1, and OPRD1. Functional enrichment analysis (FEA) of candidate target genes of Strychnosnux-vomica , Ligusticum chuanxiong and Safflowers in Longji Xiaozhong ointment in ETCM database showed SUMOylation of intracellular receptors, blockade of NMDA receptors, and activation of GABA A receptors, lipid metabolism regulationby peroxisome proliferator-activated receptor alpha (PPARα). PPARA activates aspects of gene expression and transcriptional activation of mitochondrial biogenesis. On retrospective analysis, all patients were divided into Group A: Celebrex combined with brace immobilization group (36 cases); Group B: Shexiang Jietong ointment combined with brace immobilization group (37 cases); and Group C: Longji Xiaozhong ointment combined with brace immobilization group (38 ","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"73 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"No mutation effect of 50 Hz sinusoidal magnetic field on beta catenin gene phosphorylation site in N-methyl-N-nitrosourea (MNU) induced colon tumor model","authors":"Metin Budak, Mahmut Alp K ı l ı ç, T. Kalkan, H. Tuncel","doi":"10.56042/ijeb.v61i12.4370","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.4370","url":null,"abstract":"The dysregulation of beta-catenin, a key regulator of cadherin-mediated cell adhesion and crucial for embryonic development and adult tissue processes, has been implicated in various cancers, including colon cancer. Meanwhile, there have been longstanding concerns about the potential carcinogenic effects of magnetic fields. In this study, we investigated the possible relationship between beta-catenin dysfunction and 50 Hz sinusoidal magnetic fields (SMF) using an animal model of N-methyl-N-nitrosourea (MNU)-induced rat colon tumors. To assess beta-catenin phosphorylation, genomic DNA was extracted from 58 samples using a commercial extraction kit, and the target gene region corresponding to an important phosphorylation site of beta-catenin was amplified via polymerase chain reaction (PCR). The amplified samples were subsequently analyzed using the single-strand conformation polymorphism (SSCP) method to detect any differences between the experimental groups. Surprisingly, our results revealed no significant differences in beta-catenin gene phosphorylation sites among the groups. These findings suggest that 50 Hz SMF exposure may not directly impact beta-catenin dysfunction in the context of MNU-induced rat colon tumors. Implications of these results and avenues for further research are discussed.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"23 11","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Gonadotropins on Blood, Bone Marrow, and Spleen in Cyclophosphamide-Exposed Rats","authors":"","doi":"10.56042/ijeb.v61i11.1764","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1764","url":null,"abstract":"Cyclophosphamide (CTX) is an effective chemotherapeutic agent. Gonadotropins are molecules with various actions. We investigated the effects of gonadotropins on the peripheral blood, bone marrow, and spleen in rats given CTX. Three groups were formed: Control (C) group with no process; Sham (S) group: Physiological saline was applied; CTX group: A single dose of 200 mg/kg and 8 mg/kg CTX was administered for the next 14 days. All rats were superovulated with 150-300 IU/kg pregnant mare serum gonadotropin. 150-300 IU/kg Human chorionic gonadotropin was given, and complete blood counts, bone marrow smears, and spleen sections were examined; the expression of WNT-1, WNT-4, and β-catenin was analyzed. Although the hemoglobin and platelet value in the CTX group was lowest, it was still within the normal reference ranges. The C and S groups had significantly higher white blood cell  values (p=0.017). In terms of number of megakaryocytes, Myeloid/ Erythroid  ratio, lymphoid cell ratios, no significant differences were found in bone marrow aspiration smears. The CTX group had significantly higher β-catenin expression in the red pulp than the other groups (p=0.0001). The CTX group had the highest WNT-4 expression and very intense expression of WNT-1 in the white pulp. In conclusion, gonadotropins, promising in \"treatment\", have favorable effects on toxicity of CTX.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"50 1-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of bioactive potentials in the crude flavonoid fraction from the leaves of Butea monosperma in vitro and in vivo in experimental models","authors":"","doi":"10.56042/ijeb.v61i11.1533","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1533","url":null,"abstract":"Oxidative stress is responsible for the advent of seizure hence, antioxidants are used for the treatment neurodegenerative ailments such as epilepsy. Profoundly, Butea monosperma possess several medicinal properties and the preliminary phytochemical screening revealed the presence of steroids, alkaloids, triterpenoids, polyphenols, flavonoids, tannins, and glycosides in the leaves. Thus, the present study aimed to evaluate the bioactive potentials in the isolated crude flavonoid fraction (FF) from the leaves. FF was isolated by successive methanol extract method and was evaluated for antioxidant and anticonvulsant activities in vitro and in vivo. For in vivo study, 25 healthy Wistar Albino rats were employed in five groups (Gp): Gp 1: control, Gp 2: standard group receiving phenytoin sodium and Gp 3-5: test groups receiving oral doses of FF i.e., 50, 100 and 200 mg/kg, respectively. In vitrodata showed concentration-dependent antioxidant activity (88.86±0.006%), reducing power activity (0.694±0.002%) and nitric oxide scavenging activity (87.98±0.013%). In vivo, a significant depression in the phases and percentage incidence of convulsions was observed in the maximal electroshock model in a dose-dependent manner. The pentylene tetrazole-induced seizure model also depicted a significant (p≤0.001) decrease in the latency of convulsions and an increase in the percentage protection against the convulsions in a dose-dependent manner. Our findings suggest antioxidant and anticonvulsant potential of FF obtained from Butea monosperma leaves.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"54 1-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematopoietic and Hepatoprotective Activity of Basella alba Fruit Extract in Experimental Animal Model","authors":"","doi":"10.56042/ijeb.v61i11.3650","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.3650","url":null,"abstract":"Basella alba fruit extract (BAFE) has been used in rural eastern India by the traditional practitioners to treat anemia during pregnancy. In the present study, hematopoietic and hepatoprotective activity of BAFE was established in animal model. Animal ethical permission was obtained before experiments. Male Swiss albino rats were divided into: gr.1- sham control, gr.2- disease control (anemia or hepatotoxicity), gr.3- standard drug treated, gr.4- BAFE treated (low dose) and gr.5- BAFE treated (high dose). Anemia was induced in animals through blood loss at regular intervals and hematological, cytokine, antioxidant parameters and RBC morphology were done. Hepatotoxicity was induced in animals by CCl4 (1 mL/kg/p.o.) and serum biochemistry, cytokine, antioxidant parameters and histology were done. There was a significant improvement of hematology (Hb, TC of RBC, TC of WBC, hematocrit, serum iron, TIBC), cytokine (IL 1β), antioxidant (SOD, catalase, LPO, GSH) and RBC morphology in BAFE treated animals when compared to group 2 animals. Significant improvement in biochemical (AST, ALT, γGT, ACP, ALP, total bilirubin), cytokine (IL 1β, IL 4, IL 12, IL 17, cathepsin K, TNF α), antioxidant (SOD, catalase, LPO, GSH) and liver histology were observed in BAFE treated animals when compared to group 2 animals. The present study confirmed the hematopoietic and hepatoprotective activity of BAFE in animal model and warrants further studies.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"52 3-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory effect of ethanolic extract of Ocimum basilicum L. in the healing process of incisional wounds in mice","authors":"","doi":"10.56042/ijeb.v61i11.1908","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1908","url":null,"abstract":"Maintaining the structural and functional integrity of the skin is critical to ensuring body homeostasis. After damage, a series of cellular and molecular events occurs in this structure in order to repair the injured tissue. Imbalances in one or more repair steps are commonly related to difficulties in proceeding with healing. In this sense, the search for treatments that improve the repair has encompassed research with several plants, given the possibility of effects on one or more stages, the reduction of costs and possible side effects. Ocimum basilicum, used globally from cooking to practices in folk medicine, has in its phytoconstitution substances with different actions such as anti-inflammatory, antioxidant, antimicrobial. Therefore, the present study aimed to evaluate the anti-inflammatory effect of intraperitoneal treatment with ethanolic extract of Ocimum basilicum (EEOB) on wound healing in mice. Incisional wounds were induced in mice, they were divided according to treatment period and group, n=6, and received EEOB intraperitoneally (i.p.) at 150 mg/kg daily or saline solution for 5 or 7 days. At the end of this period, the animals were euthanized and the lesions underwent histopathological processing and analysis. In animals treated with EEOB injection, there was a decrease in the number of leukocytes at 5 days and mast cells at 7 days post-injury compared to the control. The observed anti-inflammatory action can be associated with the presence of several phenolic compounds and their individual or synergistic effects on the evaluated process. Keywords: Healing; medicinal plants; inflammation.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"51 2-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effects of Diospyros Kaki Leaves Extract against Oxidative Damage and Inflammation in the Testicular Ischemia-Reperfusion Model in Rats","authors":"","doi":"10.56042/ijeb.v61i11.1916","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1916","url":null,"abstract":"Protective effect of Diospyros Kaki leaves extract against oxidative damage and inflammation in the testicular ischemia-reperfusion model were investigated in the study. 32 male rats were randomly and equally divided into 4 groups. While saline (SF) was given to the control and ischemia/reperfusion (I/R) groups for 5 days, Diospyros Kaki (DK) and I/R+DK were given 200 mg/kg of DK leaf extract. Afterwards, left orchiectomy was performed in control and DK, and testicular torsion was performed in I/R and I/R+DK for 2 hours. All rats were sacrificed 24 hours after procedures and samples were collected. Compared to the control, I/R had high MDA levels, low GSH and GSH-Px levels; compared to the I/R, MDA levels of the I/R+DK were lower, and the GSH and GSH-Px levels were higher. In I/R, the presence of congestion and hemorrhage were determined and the deterioration in seminiferous tubule structure and spermatogenesis were less in the I/R+DK. TNF-α, COX-2, BCL-2, and BAX genes expressions significantly lower in the I/R+DK. TNF-α and COX-2 proteins were higher in I/R and I/R+DK, while NRF2 was higher in I/R+DK. These results show DK leaves extract has crucial protective effect against oxidative damage and inflammation in the I/R model of rats.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"53 3-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apoptotic and antiproliferative effects of Urtica dioica L. extract on K562 chronic myeloid leukemia cell line","authors":"","doi":"10.56042/ijeb.v61i11.1574","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1574","url":null,"abstract":"Urtica dioica (stinging nettle) is an herb commonly in use as a medical supplement for its anticarcinogenic, antiinflammatory, analgesic, antifungal . In this study, phytochemical composition extract was analyzed and its apoptotic and antiproliferative effects were investigated in K562 cell line, a model cell line for chronic myeloid leukemia (CML). K562 cells exposed to different concentrations of Urtica dioica extract (0, 25, 50, 75 and 100 mg/ml) were tested for cell proliferation and appropriate methods and different phases of the cell cycle were analyzed using flow cytometry. Phenolic content of the plant was analyzed by LC–MS/MS. We showed that Urtica dioica ethanol extract has four phenolic compounds: caffeic acid, chlorogenic acid (major compound), malic acid and rutin. Urtica dioica significantly repressed proliferation of K562 cells compared to control mononuclear cells (PBMCs) isolated from the peripheral blood of healthy donors. Increase in Bax/Bcl-2 and PARP cleavage ratio, decrease in mitochondrial membrane potential indicated that the extract inhibited cell proliferation through an apoptosis-mediated process. Furthermore, cell cycle arrest at G0/G1 phase increased in a concentration dependent manner. Our results explicitly demonstrate that ethanol extract of to decrease in cell viability and proliferation, initiate apoptosis and induces G0/G1 cell cycle accumulation in this cell line. This study is the first to show antiproliferative and apoptotic effects of Urtica dioica on leukemia cell line in vitro.                                 &nbsp","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"47 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135410960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abelmoschus esculentus (L.) Moench seed extract alleviates acute acetaminophen induced liver damage in rats","authors":"","doi":"10.56042/ijeb.v61i11.1326","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1326","url":null,"abstract":"Acetaminophen (APAP) is one of the most regularly utilized medicines in children. When administered at the recommended doses, it is a safe medication. However, oxidative stress and inflammation caused by exposure to toxic doses lead to centrilobular hepatic necrosis. N-acetylcysteine (NAC) is utilized in the therapy, however it has potentially adverse effects. On the other hand, it is known that the seed of Abelmoschus esculentus (AE), a herbal product, possesses antioxidant and anti-inflammatory qualities. The aim of this study is to investigate whether AE may be used as an alternative to standart NAC therapy without fear of adverse effects in the treatment of acute APAP-induced liver injury. Forty male Wistar rats were placed into five groups: Control, AE, APAP, APAP+AE, and APAP+AE+NAC groups. Antioxidants such as native thiol and total thiol can be raised in the APAP group by taking just AE (p=0.043 and p=0.028, respectively). Anti-inflammatory indicators such as IL-10 can be increased in this group, while markers such as ALT, which is a sign of hepatotoxicity, can be decreased (p=0.005 and p<0.001, respectively). Histologically, AE has been shown to improve worsened congestion (p=0.003), cytoplasmic vacuolization (p=0.01), sinusoidal dilatation (p=0.001), Kupffer cell proliferation (p<0.001), and inflammation (p<0.001). These results suggest that AE seed may be a potential therapeutic agent for APAP-induced hepatotoxicity. The antioxidant and anti-inflammatory properties of AE seed contribute to this advantage.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"47 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135410959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of microRNA-16-5p, microRNA-194, IP-10 and APRIL in inducing inflammation in SARS-CoV-2 infected patients with severe symptoms","authors":"","doi":"10.56042/ijeb.v61i11.1922","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1922","url":null,"abstract":"The immune system is the main responsible candidate to induce pro-inflammatory conditions in the hospitalized SARS-CoV-2 infected patients. The roles played by humoral immunity-related factors in the pro-inflammatory conditions of the patients are yet to be clarified. It has been revealed that a proliferation-inducing ligand (APRIL), interferon (IFN)-γ-inducible protein 10 (IP-10), and microRNA-16-5p (miR-16-5p) play key roles in the induction of inflammation. Thus, this project was aimed to explore the expression levels of the molecules in the SARS-CoV-2 infected patients with severe symptoms. In addition, miR-194 can inhibit immune responses against viral infection. Another aim of this study was to evaluate expression of the molecule in the patients to explore the effects of the molecule during coronavirus disease 2019 (COVID-19). In this project, 60 severe SARS-CoV-2 infected patients who were in the peak of the disease and 60 healthy controls were enrolled to evaluate APRIL, IP-10, miR16-5p, and miR-194 expression levels. IP-10 expressions were evaluated using enzyme linked immunoassay (ELISA), while APRIL, miR-16-5p, and miR-194 were evaluated using Real-Time PCR technique. The results showed that APRIL, miR-16-5p, and miR-194 expression and serum levels of IP-10 significantly increased in the hospitalized SARS-CoV-2 infected patients in comparison to healthy controls. There was a positive correlation between miR-16-5p and miR-194 levels in the patients. Due to the fact that miR-16-5p significantly participates in the induction of inflammation, it, APRIL, and IP-10 may be the main parts of the excess inflammation in the hospitalized SARS-CoV-2 infected patients with severe symptoms. Up-regulation of miR-194 may be natural negative feedback to the pro-inflammatory conditions and may be associated with establishment of SARS-CoV-2 infection.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"48 2-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}