Indian journal of experimental biology最新文献_第2页

Molecular evidence of wound healing potency of lyophilized powder of Ziziphus rugosa Lam. leaf extract 叶子提取物冻干粉伤口愈合功效的分子证据

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-12-26 DOI: 10.56042/ijeb.v62i01.1615

引用次数: 0

Cloning of lichenase gene into Bacillus subtilis and partial characterization of the enzyme 将地衣酶基因克隆到枯草芽孢杆菌中并对酶进行部分鉴定

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-12-26 DOI: 10.56042/ijeb.v62i01.6512

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Effect of silver nanoparticles on antibody response against recombinant VP2 protein of Infectious bursal disease virus 银纳米粒子对针对传染性法氏囊病病毒重组 VP2 蛋白的抗体反应的影响

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-12-26 DOI: 10.56042/ijeb.v62i01.1609

引用次数: 0

Allium cepa L., a potential hepatoprotective and antituberculosis agent 薤白，一种潜在的保肝抗结核剂

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-12-26 DOI: 10.56042/ijeb.v62i01.1420

引用次数: 0

Distribution of GABPB1 gene rs7181866 polymorphism in rugby players 橄榄球运动员中 GABPB1 基因 rs7181866 多态性的分布情况

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-11-24 DOI: 10.56042/ijeb.v61i12.4003

Özlem Özge Y ı lmaz, Tolga Polat, B. Eken, Gökhan Tuna, B. Aslan, K. Ulucan

{"title":"Distribution of GABPB1 gene rs7181866 polymorphism in rugby players","authors":"Özlem Özge Y ı lmaz, Tolga Polat, B. Eken, Gökhan Tuna, B. Aslan, K. Ulucan","doi":"10.56042/ijeb.v61i12.4003","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.4003","url":null,"abstract":"Nuclear respiration factor 2 is an important respiratory factor for cellular metabolism formed by polypeptides produced by the GA binding protein transcription factor subunit alpha ( GABPA ) and GA binding protein transcription factor subunit beta 1 ( GAPPB1 ) genes. A functional polymorphism on the GABPB1 gene (rs7181866, G/A) alters the cellular respiration metabolism by affecting the protein dynamics. In this context, here, we examined the distribution of GABPB1 rs718186 polymorphism in professional rugby players to see its relationship with their stamina. Twelve rugby players participated in the study. Genotyping was performed by Real-Time PCR after DNAs were isolated from blood cells using commercially available isolation kits. In our cohort, the AA genotype and the A allele were superior to the AG and GG genotypes and the G allele. There was no statistically significant difference in terms of Genotypes (p=0.9027) and allelic groups (p=0.6948) in the athlete and control groups. Studies have revealed the relationship between endurance characteristics of athletes and rs7181866 AA genotype and A allele. These studies will be an important data source for future studies in sports genetics and GAPPB1 analysis.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"123 5","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139239444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between cervical thickness and histological severity of cervical intraepithelial neoplasia: A transvaginal ultrasound investigation 宫颈厚度与宫颈上皮内瘤变组织学严重程度之间的相关性：经阴道超声调查

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-11-24 DOI: 10.56042/ijeb.v61i12.6813

引用次数: 0

Immature mast cells predominate in skin wound repair in senescent mice 未成熟肥大细胞在衰老小鼠皮肤伤口修复中占主导地位

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-11-24 DOI: 10.56042/ijeb.v61i12.6514

引用次数: 0

Expression levels and clinical significances of hsa-miR-29 family and their target genes in the bone marrow of patients with multiple myeloma 多发性骨髓瘤患者骨髓中 hsa-miR-29 家族及其靶基因的表达水平和临床意义

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-11-24 DOI: 10.56042/ijeb.v61i12.2999

Kenan Çevik, Mustafa Ertan Ay, Özlem İzci, Anil Ay, Didem Derici Tombak, Yildirim Tuba, Kabasakal, Mehmet Emin, Erdal

{"title":"Expression levels and clinical significances of hsa-miR-29 family and their target genes in the bone marrow of patients with multiple myeloma","authors":"Kenan Çevik, Mustafa Ertan Ay, Özlem İzci, Anil Ay, Didem Derici Tombak, Yildirim Tuba, Kabasakal, Mehmet Emin, Erdal","doi":"10.56042/ijeb.v61i12.2999","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.2999","url":null,"abstract":"The microRNA (miR)-29 family has been deregulated in several types of hematologic malignancies. However, role of this family and their target genes DNMT3A (DNA methyltransferase 3A) and TET2 (Ten-Eleven Translocation 2) remains unclear. Here, we have made an attempt to determine the relative expression levels of three miRNAs and target genes in patients with newly diagnosed Multiple myeloma (MM) using quantitative real-time PCR. Moreover, the expression levels of selected miRNAs and genes and their correlations with clinical parameters were compared and analyzed. The ROC curve was used to analyze their diagnostic efficacy for MM. The expression level of hsa-miR-29b-3p was significantly higher in patients with newly diagnosed MM compared with the control group. ROC analysis showed that hsa-miR-29b-3p demonstrated a moderate diagnostic power in MM. The relative expression level of hsa-miR-29b-3p in patients with high LDH levels was markedly reduced compared to that in patients with normal and low LDH levels . DNMT3A expression level was significantly increased in patients with high LDH levels and patients with lambda light chain. Our results indicate that hsa-miR-29b-3p may be used as a potential biomarker in the diagnosis of MM.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"67 ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of Lumbricus rubellus Hoffmeister extract in experimental renal ischemia/reperfusion injury in the nephrectomy rats Lumbricus rubellus Hoffmeister 提取物对肾切除大鼠实验性肾缺血再灌注损伤的保护作用

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-11-24 DOI: 10.56042/ijeb.v61i12.1532

Semih Öz, Fatma Y ı ld ı z, Ş. A. Kabay, Hakan Ş entürk, Dilek Buruko ğ lu Dönmez, Cansu Özbayer, Mehmet Cengiz Üstüner, Bilgin Kayg ı s ı z, K. Civi, Çetin, Hilmi Özden

{"title":"Protective effect of Lumbricus rubellus Hoffmeister extract in experimental renal ischemia/reperfusion injury in the nephrectomy rats","authors":"Semih Öz, Fatma Y ı ld ı z, Ş. A. Kabay, Hakan Ş entürk, Dilek Buruko ğ lu Dönmez, Cansu Özbayer, Mehmet Cengiz Üstüner, Bilgin Kayg ı s ı z, K. Civi, Çetin, Hilmi Özden","doi":"10.56042/ijeb.v61i12.1532","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.1532","url":null,"abstract":"Acute kidney injury (AKI), an immediate loss of renal function, leads to high mortality, and ischemia-reperfusion (I/R) injury is considered as one of the main causes of AKI. Inflammation and oxidative stress are known to play an important role in AKI. On the other hand, the earthworm extract, used in traditional medicine, is known to possess various biological and pharmacological activities viz. antiapoptotic, anticoagulative, fibrinolytic, anti-inflammatory, antioxidative stress, peripheral nerve regeneration, bone regeneration and wound healing. Hence, in this study, we investigated the protective effect of the earthworm Lumbricus rubellus Hoffmeister extract (LE) after nephrectomy, against oxidative stress occurring during renal ischemia/reperfusion (I/R) injury. A total of 10-12 weeks old Sprague Dawley male rats were divided into five groups (n=8). Group I (control), Group II (I/R), Group III (I/R + 10 mg/kg LE), Group IV (I/R + 20 mg/kg LE) and Group V (I/R + 40 mg/kg LE). All rats except in Gr. I were applied ischemia for 45 min and reperfusion for 24 h. At the end of the experiment, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) enzyme activities and malondialdehyde (MDA) levels were evaluated. In addition, kidney tissues were evaluated histologically. In results, the MDA and GPx level of the I/R group were found to be significantly higher than the control and LE groups. SOD activity of the control group did not differ when compared to LE groups and CAT levels were not significantly different between all groups. In addition, in Gr. III-V we observed nearly normal renal cortex and renal tubules. The present study, thus demonstrates that the extract of L. rubellus prevents renal I/R injury and induced biochemical and histological changes in the renal tissues in rats.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"195 ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevention of dabigatran ınduced cytotoxicity by N-acetyl cysteine: An ın vitro study N-乙酰半胱氨酸预防达比加群引起的细胞毒性：体外研究

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-11-24 DOI: 10.56042/ijeb.v61i12.2738

Özer Aylin, Gürpinar, Emre Kubat

{"title":"Prevention of dabigatran ınduced cytotoxicity by N-acetyl cysteine: An ın vitro study","authors":"Özer Aylin, Gürpinar, Emre Kubat","doi":"10.56042/ijeb.v61i12.2738","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.2738","url":null,"abstract":"Dabigatran (DBG) is an oral direct thrombin inhibitor used for prevention of systemic embolism and venous thromboembolism. The major side effect of DBG is gastrointestinal upset. In the present study, we have investigated whether N-acetyl cysteine (NAC) showed a protective effect on dabigatran-induced cytotoxicity in the in vitro setting. The medium not containing DBG but containing NAC were served to assay the effect of NAC on cell proliferation and apoptosis. Comparing DAB and all other groups, the cell viability was the lowest in the D group. However, there was no statistically significant difference between the NAC I and DBG-NAC I group, while the difference was statistically significant compared to all other groups. The cells in the DBG group showed a degenerative and round-shaped morphology with nuclear condensation. In other dilutions, the cell morphology was healthy with a fibroblastic morphology. Based on our study results, NAC at high concentrations exerts cytoprotective effects against DBG, while moderate or low concentrations have no favorable effect on cell viability of NAC. Although using concomitant NAC at appropriate doses appears to be effective agent against dabigatran cytotoxicity in the current study, further experimental and clinical studies are needed to confirm our findings.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"206 ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0