Indian journal of experimental biology最新文献

{"title":"Correlation between cervical thickness and histological severity of cervical intraepithelial neoplasia: A transvaginal ultrasound investigation","authors":"","doi":"10.56042/ijeb.v61i12.6813","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.6813","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"204 ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139242387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immature mast cells predominate in skin wound repair in senescent mice","authors":"","doi":"10.56042/ijeb.v61i12.6514","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.6514","url":null,"abstract":"","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"59 16","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression levels and clinical significances of hsa-miR-29 family and their target genes in the bone marrow of patients with multiple myeloma","authors":"Kenan Çevik, Mustafa Ertan Ay, Özlem İzci, Anil Ay, Didem Derici Tombak, Yildirim Tuba, Kabasakal, Mehmet Emin, Erdal","doi":"10.56042/ijeb.v61i12.2999","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.2999","url":null,"abstract":"The microRNA (miR)-29 family has been deregulated in several types of hematologic malignancies. However, role of this family and their target genes DNMT3A (DNA methyltransferase 3A) and TET2 (Ten-Eleven Translocation 2) remains unclear. Here, we have made an attempt to determine the relative expression levels of three miRNAs and target genes in patients with newly diagnosed Multiple myeloma (MM) using quantitative real-time PCR. Moreover, the expression levels of selected miRNAs and genes and their correlations with clinical parameters were compared and analyzed. The ROC curve was used to analyze their diagnostic efficacy for MM. The expression level of hsa-miR-29b-3p was significantly higher in patients with newly diagnosed MM compared with the control group. ROC analysis showed that hsa-miR-29b-3p demonstrated a moderate diagnostic power in MM. The relative expression level of hsa-miR-29b-3p in patients with high LDH levels was markedly reduced compared to that in patients with normal and low LDH levels . DNMT3A expression level was significantly increased in patients with high LDH levels and patients with lambda light chain. Our results indicate that hsa-miR-29b-3p may be used as a potential biomarker in the diagnosis of MM.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"67 ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of Lumbricus rubellus Hoffmeister extract in experimental renal ischemia/reperfusion injury in the nephrectomy rats","authors":"Semih Öz, Fatma Y ı ld ı z, Ş. A. Kabay, Hakan Ş entürk, Dilek Buruko ğ lu Dönmez, Cansu Özbayer, Mehmet Cengiz Üstüner, Bilgin Kayg ı s ı z, K. Civi, Çetin, Hilmi Özden","doi":"10.56042/ijeb.v61i12.1532","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.1532","url":null,"abstract":"Acute kidney injury (AKI), an immediate loss of renal function, leads to high mortality, and ischemia-reperfusion (I/R) injury is considered as one of the main causes of AKI. Inflammation and oxidative stress are known to play an important role in AKI. On the other hand, the earthworm extract, used in traditional medicine, is known to possess various biological and pharmacological activities viz. antiapoptotic, anticoagulative, fibrinolytic, anti-inflammatory, antioxidative stress, peripheral nerve regeneration, bone regeneration and wound healing. Hence, in this study, we investigated the protective effect of the earthworm Lumbricus rubellus Hoffmeister extract (LE) after nephrectomy, against oxidative stress occurring during renal ischemia/reperfusion (I/R) injury. A total of 10-12 weeks old Sprague Dawley male rats were divided into five groups (n=8). Group I (control), Group II (I/R), Group III (I/R + 10 mg/kg LE), Group IV (I/R + 20 mg/kg LE) and Group V (I/R + 40 mg/kg LE). All rats except in Gr. I were applied ischemia for 45 min and reperfusion for 24 h. At the end of the experiment, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) enzyme activities and malondialdehyde (MDA) levels were evaluated. In addition, kidney tissues were evaluated histologically. In results, the MDA and GPx level of the I/R group were found to be significantly higher than the control and LE groups. SOD activity of the control group did not differ when compared to LE groups and CAT levels were not significantly different between all groups. In addition, in Gr. III-V we observed nearly normal renal cortex and renal tubules. The present study, thus demonstrates that the extract of L. rubellus prevents renal I/R injury and induced biochemical and histological changes in the renal tissues in rats.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"195 ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevention of dabigatran ınduced cytotoxicity by N-acetyl cysteine: An ın vitro study","authors":"Özer Aylin, Gürpinar, Emre Kubat","doi":"10.56042/ijeb.v61i12.2738","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.2738","url":null,"abstract":"Dabigatran (DBG) is an oral direct thrombin inhibitor used for prevention of systemic embolism and venous thromboembolism. The major side effect of DBG is gastrointestinal upset. In the present study, we have investigated whether N-acetyl cysteine (NAC) showed a protective effect on dabigatran-induced cytotoxicity in the in vitro setting. The medium not containing DBG but containing NAC were served to assay the effect of NAC on cell proliferation and apoptosis. Comparing DAB and all other groups, the cell viability was the lowest in the D group. However, there was no statistically significant difference between the NAC I and DBG-NAC I group, while the difference was statistically significant compared to all other groups. The cells in the DBG group showed a degenerative and round-shaped morphology with nuclear condensation. In other dilutions, the cell morphology was healthy with a fibroblastic morphology. Based on our study results, NAC at high concentrations exerts cytoprotective effects against DBG, while moderate or low concentrations have no favorable effect on cell viability of NAC. Although using concomitant NAC at appropriate doses appears to be effective agent against dabigatran cytotoxicity in the current study, further experimental and clinical studies are needed to confirm our findings.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"206 ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular targets and mechanism of Longji Xiaozhong ointment in treating acute ankle sprain: A network pharmacology based analysis","authors":"Yumei Li, Qing Zhang, Haixia Zhu, De-ta Chen, Tian-you Fan","doi":"10.56042/ijeb.v61i12.6811","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.6811","url":null,"abstract":"Acute ankle sprain is one of the most common lower limb injuries particularly in athletes and it accounts for 16-40% of all sports-related injuries. A significant proportion of patients experience persistent residual symptoms and recurrence. Traditional Chinese medicine (TCM) offers effective treatment in terms of pain relief, swelling reduction, functional improvement, and shorter disease duration. This study provides a comprehensive analysis of the active ingredients, molecular targets, signaling pathways, and Longji Xiaozhong ointment's clinical efficacy for treating acute ankle sprains. We followed network pharmacology approach to identify the molecular targets and mechanism of action of Longji Xiaozhong ointment in treating acute ankle sprain and to validate its clinical value using relevant clinical data. Initially, Longji Xiaozhong Ointment's active ingredients were screened using the TCMSP platform based on drug properties similar to DLP ≥0.18 and oral bioavailability OB ≥30%. The molecular targets were identified from the ETCM database, followed by constructing protein-protein interaction (PPI) network diagrams and GO and pathway enrichment analysis on key genes. Furthermore, the UniProt database was used to investigate the 3D structures of these key genes. Finally, the clinical data of 111 patients with acute ankle sprains were retrospectively analyzed and further explored by comparing the different curative effects of Longji Xiaozhong ointment with Celebrex and Shexiang Analgesic ointment to check the clinical value of Longji Xiaozhong ointment in treating acute ankle sprain. In the TCMSP database, we found that there were 42 active ingredients in Longji Xiaozhong ointment, including 13 kinds of Strychnosnux-vomica L., 7 kinds of Ligusticum chuanxiong , and 22 kinds of Safflowers ( Carthamus tinctorius ), and a total of 3206 target genes were obtained. After applying the OB ≥50% criteriaand DL ≥0.5, 19 key target genes were selected based on their correspondence to the active ingredients. Protein mutual aid network construction and module analysis yielded two high-scoring Clusters and identified seven key proteins, including SLC6A4, ADRB2, ADRA1B, CHRM1, F2, OPRM1, and OPRD1. Functional enrichment analysis (FEA) of candidate target genes of Strychnosnux-vomica , Ligusticum chuanxiong and Safflowers in Longji Xiaozhong ointment in ETCM database showed SUMOylation of intracellular receptors, blockade of NMDA receptors, and activation of GABA A receptors, lipid metabolism regulationby peroxisome proliferator-activated receptor alpha (PPARα). PPARA activates aspects of gene expression and transcriptional activation of mitochondrial biogenesis. On retrospective analysis, all patients were divided into Group A: Celebrex combined with brace immobilization group (36 cases); Group B: Shexiang Jietong ointment combined with brace immobilization group (37 cases); and Group C: Longji Xiaozhong ointment combined with brace immobilization group (38 ","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"73 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"No mutation effect of 50 Hz sinusoidal magnetic field on beta catenin gene phosphorylation site in N-methyl-N-nitrosourea (MNU) induced colon tumor model","authors":"Metin Budak, Mahmut Alp K ı l ı ç, T. Kalkan, H. Tuncel","doi":"10.56042/ijeb.v61i12.4370","DOIUrl":"https://doi.org/10.56042/ijeb.v61i12.4370","url":null,"abstract":"The dysregulation of beta-catenin, a key regulator of cadherin-mediated cell adhesion and crucial for embryonic development and adult tissue processes, has been implicated in various cancers, including colon cancer. Meanwhile, there have been longstanding concerns about the potential carcinogenic effects of magnetic fields. In this study, we investigated the possible relationship between beta-catenin dysfunction and 50 Hz sinusoidal magnetic fields (SMF) using an animal model of N-methyl-N-nitrosourea (MNU)-induced rat colon tumors. To assess beta-catenin phosphorylation, genomic DNA was extracted from 58 samples using a commercial extraction kit, and the target gene region corresponding to an important phosphorylation site of beta-catenin was amplified via polymerase chain reaction (PCR). The amplified samples were subsequently analyzed using the single-strand conformation polymorphism (SSCP) method to detect any differences between the experimental groups. Surprisingly, our results revealed no significant differences in beta-catenin gene phosphorylation sites among the groups. These findings suggest that 50 Hz SMF exposure may not directly impact beta-catenin dysfunction in the context of MNU-induced rat colon tumors. Implications of these results and avenues for further research are discussed.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"23 11","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Gonadotropins on Blood, Bone Marrow, and Spleen in Cyclophosphamide-Exposed Rats","authors":"","doi":"10.56042/ijeb.v61i11.1764","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1764","url":null,"abstract":"Cyclophosphamide (CTX) is an effective chemotherapeutic agent. Gonadotropins are molecules with various actions. We investigated the effects of gonadotropins on the peripheral blood, bone marrow, and spleen in rats given CTX. Three groups were formed: Control (C) group with no process; Sham (S) group: Physiological saline was applied; CTX group: A single dose of 200 mg/kg and 8 mg/kg CTX was administered for the next 14 days. All rats were superovulated with 150-300 IU/kg pregnant mare serum gonadotropin. 150-300 IU/kg Human chorionic gonadotropin was given, and complete blood counts, bone marrow smears, and spleen sections were examined; the expression of WNT-1, WNT-4, and β-catenin was analyzed. Although the hemoglobin and platelet value in the CTX group was lowest, it was still within the normal reference ranges. The C and S groups had significantly higher white blood cell  values (p=0.017). In terms of number of megakaryocytes, Myeloid/ Erythroid  ratio, lymphoid cell ratios, no significant differences were found in bone marrow aspiration smears. The CTX group had significantly higher β-catenin expression in the red pulp than the other groups (p=0.0001). The CTX group had the highest WNT-4 expression and very intense expression of WNT-1 in the white pulp. In conclusion, gonadotropins, promising in \"treatment\", have favorable effects on toxicity of CTX.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"50 1-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of bioactive potentials in the crude flavonoid fraction from the leaves of Butea monosperma in vitro and in vivo in experimental models","authors":"","doi":"10.56042/ijeb.v61i11.1533","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.1533","url":null,"abstract":"Oxidative stress is responsible for the advent of seizure hence, antioxidants are used for the treatment neurodegenerative ailments such as epilepsy. Profoundly, Butea monosperma possess several medicinal properties and the preliminary phytochemical screening revealed the presence of steroids, alkaloids, triterpenoids, polyphenols, flavonoids, tannins, and glycosides in the leaves. Thus, the present study aimed to evaluate the bioactive potentials in the isolated crude flavonoid fraction (FF) from the leaves. FF was isolated by successive methanol extract method and was evaluated for antioxidant and anticonvulsant activities in vitro and in vivo. For in vivo study, 25 healthy Wistar Albino rats were employed in five groups (Gp): Gp 1: control, Gp 2: standard group receiving phenytoin sodium and Gp 3-5: test groups receiving oral doses of FF i.e., 50, 100 and 200 mg/kg, respectively. In vitrodata showed concentration-dependent antioxidant activity (88.86±0.006%), reducing power activity (0.694±0.002%) and nitric oxide scavenging activity (87.98±0.013%). In vivo, a significant depression in the phases and percentage incidence of convulsions was observed in the maximal electroshock model in a dose-dependent manner. The pentylene tetrazole-induced seizure model also depicted a significant (p≤0.001) decrease in the latency of convulsions and an increase in the percentage protection against the convulsions in a dose-dependent manner. Our findings suggest antioxidant and anticonvulsant potential of FF obtained from Butea monosperma leaves.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"54 1-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematopoietic and Hepatoprotective Activity of Basella alba Fruit Extract in Experimental Animal Model","authors":"","doi":"10.56042/ijeb.v61i11.3650","DOIUrl":"https://doi.org/10.56042/ijeb.v61i11.3650","url":null,"abstract":"Basella alba fruit extract (BAFE) has been used in rural eastern India by the traditional practitioners to treat anemia during pregnancy. In the present study, hematopoietic and hepatoprotective activity of BAFE was established in animal model. Animal ethical permission was obtained before experiments. Male Swiss albino rats were divided into: gr.1- sham control, gr.2- disease control (anemia or hepatotoxicity), gr.3- standard drug treated, gr.4- BAFE treated (low dose) and gr.5- BAFE treated (high dose). Anemia was induced in animals through blood loss at regular intervals and hematological, cytokine, antioxidant parameters and RBC morphology were done. Hepatotoxicity was induced in animals by CCl4 (1 mL/kg/p.o.) and serum biochemistry, cytokine, antioxidant parameters and histology were done. There was a significant improvement of hematology (Hb, TC of RBC, TC of WBC, hematocrit, serum iron, TIBC), cytokine (IL 1β), antioxidant (SOD, catalase, LPO, GSH) and RBC morphology in BAFE treated animals when compared to group 2 animals. Significant improvement in biochemical (AST, ALT, γGT, ACP, ALP, total bilirubin), cytokine (IL 1β, IL 4, IL 12, IL 17, cathepsin K, TNF α), antioxidant (SOD, catalase, LPO, GSH) and liver histology were observed in BAFE treated animals when compared to group 2 animals. The present study confirmed the hematopoietic and hepatoprotective activity of BAFE in animal model and warrants further studies.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"52 3-4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}