Indian journal of experimental biology最新文献

Naringenin prevents diabetic retinopathy via inhibition of apoptosis, oxidative stress and inflammation through heme oxygenase-1 upregulation 柚皮素通过血红素氧化酶-1上调抑制细胞凋亡、氧化应激和炎症来预防糖尿病视网膜病变

4区生物学

Indian journal of experimental biology Pub Date : 2025-01-01 DOI: 10.56042/ijeb.v63i08.17433

Lu Ji, Huiqin Lu, Aping Wu

{"title":"Naringenin prevents diabetic retinopathy via inhibition of apoptosis, oxidative stress and inflammation through heme oxygenase-1 upregulation","authors":"Lu Ji, Huiqin Lu, Aping Wu","doi":"10.56042/ijeb.v63i08.17433","DOIUrl":"https://doi.org/10.56042/ijeb.v63i08.17433","url":null,"abstract":"Despite the high prevalence of diabetic retinopathy, early interventions remain limited due to a lack of therapies targeting oxidative stress and inflammation simultaneously. This study investigates naringenin, a flavonoid with potential antioxidant and anti-inflammatory properties, as a novel therapeutic candidate targeting HO-1 upregulation to mitigate retinal damage. In this regard, a rat model of streptozocin-induced diabetic retinopathy was established and naringenin was administrated (40 mg/Kg/day and 80 mg/Kg/day). Two other untreated groups of diabetic and non-diabetic rats were used to compare with naringenin-treated groups. The findings revealed that naringenin decreased the overexpression of the pro-inflammatory factors IL-1β, IL-6, and TNF-α in the retinal tissue. Moreover, naringenin inhibited the overexpression of TLR4, NF-κB, and CASP-3, caused heme oxygenase-1 (HO-1) overexpression, upregulated BCL-2, reduced the levels of malondialdehyde, and elevated the levels of superoxide dismutase, catalase, and glutathione peroxidase (P-value<0.05). Intraperitoneal injection of the HO-1 inhibitor zinc protoporphyrin (ZnPP) blocked the protective effect of naringenin. These findings suggest that naringenin exerts therapeutic effects in diabetic retinopathy possibly by inducing HO-1 expression.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://or.niscpr.res.in/index.php/IJEB/article/download/17433/5028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147922494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osteomodulin silencing alleviates renal podocyte injury in membranous nephropathy by inhibiting pyroptosis 骨调节素沉默通过抑制焦亡减轻膜性肾病肾足细胞损伤

4区生物学

Indian journal of experimental biology Pub Date : 2025-01-01 DOI: 10.56042/ijeb.v63i08.17447

Daoyuan Lv, Yuqing Su, Laping Chu, Yuan Du, Neng Bao, Yanlie Zheng, Yafen Yu

{"title":"Osteomodulin silencing alleviates renal podocyte injury in membranous nephropathy by inhibiting pyroptosis","authors":"Daoyuan Lv, Yuqing Su, Laping Chu, Yuan Du, Neng Bao, Yanlie Zheng, Yafen Yu","doi":"10.56042/ijeb.v63i08.17447","DOIUrl":"https://doi.org/10.56042/ijeb.v63i08.17447","url":null,"abstract":"Membranous nephropathy (MN) is an autoimmune disorder primarily characterized by renal podocyte injury. The emerging role of pyroptosis, a novel form of regulated cell death, in the pathogenesis of podocyte damage in MN underscores the need to identify additional regulators and inhibitors of pyroptosis to optimize MN treatment. This study examined osteomodulin (OMD) as a potential regulator of pyroptosis in MN using a passive Heymann nephritis (PHN) rat model. OMD silencing in PHN rats resulted in an alleviation of nephrotic syndrome, as evidenced by reductions in 24-h urine protein and low-density lipoprotein levels, and increased albumin levels. Additionally, OMD silencing mitigated podocyte injury, as indicated by the restoration of podocyte foot process architecture, enhanced expression and normalization of Nephrin and Podocin, and reduced Desmin expression in glomeruli. Mechanistically, OMD silencing led to the inhibition of renal p38 MAPK activation and pyroptosis, demonstrated by decreased expression of intrarenal p-p38 MAPK (Thr180/Tyr182) and key pyroptosis markers, including NLRP3, ASC, Caspase-1, Caspase-1 p20, IL-1β, IL-18, GSDMD, and GSDMD-N, as well as lowered serum levels of IL-1β and IL-18. In conclusion, this study suggests that OMD silencing attenuates renal podocyte injury in MN through inhibiting pyroptosis, highlighting a promising new therapeutic approach for MN.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://or.niscpr.res.in/index.php/IJEB/article/download/17447/5029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147906734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taurine reduces cholesterol through CYP7A1 in a calcineurin-dependent manner 牛磺酸以钙调磷酸酶依赖的方式通过CYP7A1降低胆固醇

4区生物学

Indian journal of experimental biology Pub Date : 2024-01-01 DOI: 10.56042/ijeb.v62i10.371

Junxia Guo, Tong Ou, Ya Gao, Yuxing Zhao, Jing Zhang, Yanzhen Zhang, Wen Chen

{"title":"Taurine reduces cholesterol through CYP7A1 in a calcineurin-dependent manner","authors":"Junxia Guo, Tong Ou, Ya Gao, Yuxing Zhao, Jing Zhang, Yanzhen Zhang, Wen Chen","doi":"10.56042/ijeb.v62i10.371","DOIUrl":"https://doi.org/10.56042/ijeb.v62i10.371","url":null,"abstract":"Taurine (2-aminoethanesulfonic acid) could reduce serum and liver cholesterol concentrations via regulating expression and the activity of cholesterol 7α-hydroxylase (CYP7A1). To investigate the possible role of calcineurin in taurine upregulating CYP7A1 expression in HepG2 cells under high cholesterol conditions. High cellular cholesterol conditions were achieved using 0.2 mM cholesterol in HepG2 cells. Calcineurin was decreased by FK506 and was depleted in CnAβ-/- cells. HepG2 cells were cultured in a taurine-containing medium for 24 or 48 h. The levels of total intracellular cholesterol were determined by an enzymatic method, and the expression levels of calcineurin, CYP7A1, and key transcriptional regulatory molecules were detected by western blotting. High-cholesterol resulted in increased CYP7A1 and calcineurin expressions. Taurine exhibited cholesterol-lowering effects regardless of low/high cholesterol conditions or calcineurin status. Taurine induced the expression of CYP7A1, which was abolished by inhibiting or deleting calcineurin. Taurine suppressed MEK1/2, p-c-Jun, and SHP-1, key molecules in one inhibitory pathway of CYP7A1 transcription. In contrast, suppression of MEK1/2 but not p-c-Jun or SHP-1 was reversed after completely knocking down calcineurin. Calcineurin is required for taurine’s upregulation of CYP7A1 expression through inhibiting MEK1/2, which was partly responsible for taurine’s cholesterol-lowering effect.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://or.niscpr.res.in/index.php/IJEB/article/download/371/3453","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147920634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sini Decoction containing serum modulates the expression of angiogenesis-associated molecules in HUVECs by regulating the signalling pathway VEGF-Dll4/Notch1 and p38MAPK/ERK1/2/p-AKT 含血清四逆汤通过调节信号通路VEGF-Dll4/Notch1和p38MAPK/ erk1 /p-AKT调节HUVECs血管生成相关分子的表达

4区生物学

Indian journal of experimental biology Pub Date : 2024-01-01 DOI: 10.56042/ijeb.v62i12.6850

Yalan Qiu, Qing Song, Yunyi Zhai, Lei Wang, Binbin Cheng, Jingdong Gao

{"title":"Sini Decoction containing serum modulates the expression of angiogenesis-associated molecules in HUVECs by regulating the signalling pathway VEGF-Dll4/Notch1 and p38MAPK/ERK1/2/p-AKT","authors":"Yalan Qiu, Qing Song, Yunyi Zhai, Lei Wang, Binbin Cheng, Jingdong Gao","doi":"10.56042/ijeb.v62i12.6850","DOIUrl":"https://doi.org/10.56042/ijeb.v62i12.6850","url":null,"abstract":"Angiogenesis is crucial for tumour growth and metastasis. Vascular endothelial growth factor (VEGF) and Notch ligand Delta-like 4 (Dll4) play a critical role in vessel formation and angiogenesis. This study aimed to determine anti-angiogenesis effects of Sini Decoction serum on human umbilical vein endothelial cells (HUVECs). We prepared Sini Decoction serum in doses of 0.925, 1.85, 3.7 g/mL, and administered to HUVECs. HUVECs migration was evaluated using a wound healing assay. Formation of tube-like structures in HUVECs was observed using a tube-formation assay. Gene transcriptions of Dll4, Notch1, VEGF receptor 2 (VEGFR-2) and VEGF were examined using a PCR assay. Protein expressions of Janus kinase-2/signal transducer and activator of transcription 3 (JAK2/STAT3) molecules and signal molecules downstream of VEGF/VEGFR related to angiogenesis (including mitogen-activated protein kinase (p38MAPK), extracellular signal-regulated kinase-1/2 (ERK1/2), focal adhesion kinase (FAK) and phosphorylated protein kinase B (p-Akt)) were detected by western blot. Sini Decoction serum significantly inhibited cell migration and the formation of tube-shaped structures in HUVECs compared to Control group (P<0.05). Sini Decoction serum markedly decreased Dll4, Notch1, VEGF, and increased VEGFR-2 gene transcriptions, compared to those of Control group (P<0.05). Sini Decoction serum significantly reduced expression of p-Akt, p38MAPK and ERK1/2, compared to Control group (P<0.05). Although a reduction in FAK and STAT3 expression was discovered in HUVECs administered by Sini Decoction serum, without statistical significance compared to Control group (P>0.05). In conclusion, Sini Decoction serum modulated the expression of angiogenesis-associated molecules in HUVECs by activating VEGF-Dll4/Notch1 and p38MAPK/ERK1/2/p-AKT signalling pathways.","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://or.niscpr.res.in/index.php/IJEB/article/download/6850/3823","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shennao fuyuan decoction and hUCMSC-exo combination therapy promotes lipid and atherosclerosis pathway activation and improves brain injury in middle cerebral artery occlusion rats 参脑复元汤联合hUCMSC-exo联合治疗可促进大脑中动脉闭塞大鼠脂质及动脉粥样硬化通路激活，改善脑损伤

4区生物学

Indian journal of experimental biology Pub Date : 2024-01-01 DOI: 10.56042/ijeb.v62i08.5134

Xumin Li, Yan Shang, Lihua Xie, Guoheng Hu

{"title":"Shennao fuyuan decoction and hUCMSC-exo combination therapy promotes lipid and atherosclerosis pathway activation and improves brain injury in middle cerebral artery occlusion rats","authors":"Xumin Li, Yan Shang, Lihua Xie, Guoheng Hu","doi":"10.56042/ijeb.v62i08.5134","DOIUrl":"https://doi.org/10.56042/ijeb.v62i08.5134","url":null,"abstract":"Human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-exo) decrease the mortality rates associated with brain injuries. Its combination with Shennao fuyuan decoction (SFD), a traditional Chinese medicine formulation for ischemic stroke treatment, additionally augments neural deficit repair in rats post-cerebral ischemia. Nonetheless, there is a notable need for more comprehensive research into the exact mechanisms by which this combined treatment ameliorates brain injuries. Consequently, we conducted thorough research on the mechanisms of action of these entities, utilizing network pharmacology approaches and a rat model of middle cerebral artery occlusion (MCAO). Our findings identified the hsa05417: Lipid and atherosclerosis pathway as the primary channel through which SFD impacts the cardiovascular system. The integrated treatment of SFD and hUCMSC-exo attenuated TNF and HSP90AA1 expression and elevated IL6 and GSK3B levels in the brains of rats subjected to MCAO. Moreover, the discrete utilization of hUCMSC-exo in conjunction with SFD reduced cerebral infarction and diminished tissue damage in rats with MCAO. Our investigation has revealed that the coordinated use of hUCMSC-exo and SFD may influence the TNF, HSP90AA1, IL6 and GSK3B targets, thereby managing the lipid and atherosclerosis pathway and lessening cerebral harm in rats afflicted by MCAO","PeriodicalId":13290,"journal":{"name":"Indian journal of experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://or.niscpr.res.in/index.php/IJEB/article/download/5134/3231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147902920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of everolimus combined with anastrozole on serum FOXP 3 and MMP 9 in hormone receptor-positive elderly breast cancer patients 依维莫司联合阿那曲唑对激素受体阳性老年乳腺癌患者血清 FOXP 3 和 MMP 9 的影响

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-12-26 DOI: 10.56042/ijeb.v62i01.7446

引用次数: 0

Bioefficacy of marine seaweed Sargassum wightii Greville ex J.Agardh extracts on the growth of Bombyx mori (L.) larvae 海洋海藻 Sargassum wightii Greville ex J.Agardh 提取物对沙蚕幼虫生长的生物功效

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-12-26 DOI: 10.56042/ijeb.v62i01.1149

引用次数: 0

Antibiotic resistance profiles of Escherichia coli isolated from the floating islands and water of Çat Dam Lake, Adiyaman, Turkey 从土耳其阿迪亚曼恰特坝湖浮岛和水中分离的大肠埃希菌的抗生素耐药性概况

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-12-26 DOI: 10.56042/ijeb.v62i01.4679

引用次数: 0

Potential drug candidates against Hepatitis C virus: In silico screening of phytoconstituents from Phyllanthus fraternus G.L. Webster 抗丙型肝炎病毒的潜在候选药物：从 Phyllanthus fraternus G.L. Webster 中提取的植物成分的硅学筛选

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-12-26 DOI: 10.56042/ijeb.v62i01.4628

引用次数: 0

Effect of combination of radiotherapy and docetaxel on Cripto-1, β-catenin and DBC1 expression in breast cancer patients 放疗和多西他赛联合疗法对乳腺癌患者体内 Cripto-1、β-catenin 和 DBC1 表达的影响

IF 0.6 4区生物学

Indian journal of experimental biology Pub Date : 2023-12-26 DOI: 10.56042/ijeb.v62i01.7444

引用次数: 0