Abelmoschus esculentus (L.) Moench seed extract alleviates acute acetaminophen induced liver damage in rats

Abstract

Acetaminophen (APAP) is one of the most regularly utilized medicines in children. When administered at the recommended doses, it is a safe medication. However, oxidative stress and inflammation caused by exposure to toxic doses lead to centrilobular hepatic necrosis. N-acetylcysteine (NAC) is utilized in the therapy, however it has potentially adverse effects. On the other hand, it is known that the seed of Abelmoschus esculentus (AE), a herbal product, possesses antioxidant and anti-inflammatory qualities. The aim of this study is to investigate whether AE may be used as an alternative to standart NAC therapy without fear of adverse effects in the treatment of acute APAP-induced liver injury. Forty male Wistar rats were placed into five groups: Control, AE, APAP, APAP+AE, and APAP+AE+NAC groups. Antioxidants such as native thiol and total thiol can be raised in the APAP group by taking just AE (p=0.043 and p=0.028, respectively). Anti-inflammatory indicators such as IL-10 can be increased in this group, while markers such as ALT, which is a sign of hepatotoxicity, can be decreased (p=0.005 and p<0.001, respectively). Histologically, AE has been shown to improve worsened congestion (p=0.003), cytoplasmic vacuolization (p=0.01), sinusoidal dilatation (p=0.001), Kupffer cell proliferation (p<0.001), and inflammation (p<0.001). These results suggest that AE seed may be a potential therapeutic agent for APAP-induced hepatotoxicity. The antioxidant and anti-inflammatory properties of AE seed contribute to this advantage.