Hypertension: Journal of the American Heart Association最新文献_第5页

Linkage of Essential Hypertension to Chromosome 18q 原发性高血压与18q染色体的连锁关系

Hypertension: Journal of the American Heart Association Pub Date : 2002-06-01 DOI: 10.1161/01.HYP.0000018580.24644.18

K. Kristjánsson, A. Manolescu, A. Kristinsson, T. Hardarson, H. Knudsen, Sigurdur Ingason, G. Thorleifsson, M. Frigge, A. Kong, J. Gulcher, K. Stefánsson

引用次数: 101

Prediction of Outcomes in Hypertensive Patients With Suspected Coronary Disease 高血压疑似冠心病患者预后的预测

Hypertension: Journal of the American Heart Association Pub Date : 2002-06-01 DOI: 10.1161/01.HYP.0000018040.27363.53

T. Marwick, C. Case, S. Sawada, C. Vasey, James D. Thomas

{"title":"Prediction of Outcomes in Hypertensive Patients With Suspected Coronary Disease","authors":"T. Marwick, C. Case, S. Sawada, C. Vasey, James D. Thomas","doi":"10.1161/01.HYP.0000018040.27363.53","DOIUrl":"https://doi.org/10.1161/01.HYP.0000018040.27363.53","url":null,"abstract":"Stress echocardiography has been shown to improve the diagnosis of coronary artery disease in the presence of hypertension, but its value in prognostic evaluation is unclear. We sought to determine whether stress echocardiography could be used to predict mortality in 2363 patients with hypertension, who were followed for up to 10 years (mean 4.0±1.8) for death and revascularization. Stress echocardiograms were normal in 1483 patients (63%), 16% had resting left ventricular (LV) dysfunction alone, and 21% had ischemia. Abnormalities were confined to one territory in 489 patients (21%) and to multiple territories in 365 patients (15%). Cardiac death was less frequent among the patients able to exercise than among those undergoing dobutamine echocardiography (4% versus 7%, P < 0.001). The risk of death in patients with a negative stress echocardiogram was <1% per year. Ischemia identified by stress echocardiography was an independent predictor of mortality in those able to exercise (hazard ratio 2.21, 95% confidence intervals 1.10 to 4.43, P =0.0001) as well as those undergoing dobutamine echo (hazard ratio 2.39, 95% confidence intervals 1.53 to 3.75, P =0.0001); other predictors were age, heart failure, resting LV dysfunction, and the Duke treadmill score. In stepwise models replicating the sequence of clinical evaluation, the results of stress echocardiography added prognostic power to models based on clinical and stress-testing variables. Thus, the results of stress echocardiography are an independent predictor of cardiac death in hypertensive patients with known or suspected coronary artery disease, incremental to clinical risks and exercise results.","PeriodicalId":13233,"journal":{"name":"Hypertension: Journal of the American Heart Association","volume":"4 1","pages":"1113-1118"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81640012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Socioeconomic Inequalities in Hypertension Prevalence and Care: The IHPAF Study 高血压患病率和治疗中的社会经济不平等:IHPAF研究

Hypertension: Journal of the American Heart Association Pub Date : 2002-06-01 DOI: 10.1161/01.HYP.0000018912.05345.55

R. de Gaudemaris, T. Lang, G. Chatellier, L. Larabi, V. Lauwers-Cances, A. Maître, E. Diène

{"title":"Socioeconomic Inequalities in Hypertension Prevalence and Care: The IHPAF Study","authors":"R. de Gaudemaris, T. Lang, G. Chatellier, L. Larabi, V. Lauwers-Cances, A. Maître, E. Diène","doi":"10.1161/01.HYP.0000018912.05345.55","DOIUrl":"https://doi.org/10.1161/01.HYP.0000018912.05345.55","url":null,"abstract":"To analyze the health disparities relative to the prevalence of arterial hypertension and its therapeutic control in the active French population, in relation to occupational categories (OC), a population of 17 359 men and 12 267 women was assessed from January 1997 to April 1998. The initial phase was a cross-sectional analysis of a cohort study designed to assess the incidence of arterial hypertension in a French working population. Information was collected by the worksite physician during the annual examination. Blood pressure (BP) was measured using a validated automatic device. Among subjects with a BP ≥140/90 mm Hg, patients not treated with antihypertensive drugs were invited to have an additional BP measurement, 1 month later. Overall prevalence was 16.1% for men and 9.4% for women. Both prevalence and therapeutic control of high BP were related to OCs in this study. Prevalence of hypertension was higher and maintenance of therapeutic control lower among lower OCs. In contrast, awareness of high BP and the proportion of hypertensive subjects under current treatment were not related to OCs. Educational level and low OC were significantly related to prevalence of high BP after adjustment for obesity, excessive alcohol consumption, and sedentary lifestyle in women only. A poor BP control under treatment was related to high alcohol intake and low OC in men. In women only, however, low educational level was related to high prevalence of hypertension and poor BP control under antihypertensive treatment. Inequalities in hypertension prevalence persist, with prevalence being higher among lower OCs. Social disparities were not observed, however, in awareness of their condition among hypertensive subjects and among patients for receiving versus not receiving any treatment for hypertension. In contrast, BP control under antihypertensive treatment was lower among lower OCs.","PeriodicalId":13233,"journal":{"name":"Hypertension: Journal of the American Heart Association","volume":"4 1","pages":"1119-1125"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90501565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 128

Urinary Protein and Essential Hypertension in Black and in White People 黑人和白人尿蛋白与原发性高血压的关系

Hypertension: Journal of the American Heart Association Pub Date : 2002-06-01 DOI: 10.1161/01.HYP.0000019129.44960.C0

R. Chelliah, G. Sagnella, N. Markandu, G. MacGregor

{"title":"Urinary Protein and Essential Hypertension in Black and in White People","authors":"R. Chelliah, G. Sagnella, N. Markandu, G. MacGregor","doi":"10.1161/01.HYP.0000019129.44960.C0","DOIUrl":"https://doi.org/10.1161/01.HYP.0000019129.44960.C0","url":null,"abstract":"The objectives of this work were to examine the association between urinary protein and blood pressure and to compare the pattern of urinary protein excretion with essential hypertension in people of European origin (whites) and in people of African or African-Caribbean origin (blacks) living in southwest London, United Kingdom. In the groups as a whole, there were no significant differences in total urinary protein excretion between blacks and whites (geometric means [95% CI]: 94.0 [85.9 to 102.9] mg/24h for the blacks [n=151] and 102.1 [96.1 to 108.4] mg/24h for the whites [n= 219]). There were also no significant differences between blacks and whites in urinary albumin (6.5 [4.9 to 8.5] mg/24h for the blacks [n=97] and 7.1 [5.6 to 9.0] mg/24h for the whites [n=123]). In both groups, those with essential hypertension displayed a significantly raised urinary protein excretion (1.21-fold higher for the blacks and 1.19-fold higher for the whites) and albumin excretion (1.69-fold higher for the blacks and 2.40-fold higher for the whites). Urinary transferrin excretion measured in a subgroup of 67 subjects was also raised in those with essential hypertension (3.22-fold higher in the blacks and 2.76-fold higher in the whites). Examination of urinary proteins by SDS-PAGE did not identify any pattern consistent with a reduction in renal tubular protein reabsorption in those with essential hypertension. These results suggest that the increase in protein excretion in essential hypertension could be due, at least in part, to an increase in glomerular protein ultrafiltration.","PeriodicalId":13233,"journal":{"name":"Hypertension: Journal of the American Heart Association","volume":"2 1","pages":"1064-1070"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88555936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Nitric Oxide Modulates Superoxide Release and Peroxynitrite Formation in Human Blood Vessels 一氧化氮调节人体血管中超氧化物释放和过氧亚硝酸盐形成

Hypertension: Journal of the American Heart Association Pub Date : 2002-06-01 DOI: 10.1161/01.HYP.0000018041.48432.B5

T. Guzik, N. West, R. Pillai, D. Taggart, K. Channon

{"title":"Nitric Oxide Modulates Superoxide Release and Peroxynitrite Formation in Human Blood Vessels","authors":"T. Guzik, N. West, R. Pillai, D. Taggart, K. Channon","doi":"10.1161/01.HYP.0000018041.48432.B5","DOIUrl":"https://doi.org/10.1161/01.HYP.0000018041.48432.B5","url":null,"abstract":"Nitric oxide and superoxide have important roles as vascular signaling molecules. Nitric oxide (NO) reacts rapidly with superoxide, producing peroxynitrite. The relative balance between these radicals has important implications for vascular pathophysiology in hypertension and other vascular disease states. However, the relationships between superoxide, NO, and peroxynitrite formation in human blood vessels remain unclear. Accordingly, we systematically measured NO, superoxide, and peroxynitrite production from human internal mammary arteries, radial arteries, and saphenous veins from 78 patients undergoing coronary bypass surgery. Basal superoxide release was detected in all vessels at similar levels. However, endothelial removal or nitric oxide synthase inhibition increased mean superoxide release, with a corresponding reduction in peroxynitrite formation. Conversely, NO donors and superoxide scavengers both reduced superoxide release, whereas only NO donors increased peroxynitrite formation. These changes were much larger in arteries that in veins, but there were striking correlations between superoxide production, NO bioavailability, and peroxynitrite formation between the vessel types. Our findings provide direct evidence for coordinated vascular signaling mediated by interactions between NO, superoxide, and peroxynitrite and have important implications for studies of the functional effects of these radicals in human blood vessels.","PeriodicalId":13233,"journal":{"name":"Hypertension: Journal of the American Heart Association","volume":"24 1","pages":"1088-1094"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83834923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 251

ADD1 460W Allele Associated With Cardiovascular Disease in Hypertensive Individuals ADD1 460W等位基因与高血压患者心血管疾病相关

Hypertension: Journal of the American Heart Association Pub Date : 2002-06-01 DOI: 10.1161/01.HYP.0000019128.94483.3A

A. Morrison, M. Bray, A. Folsom, E. Boerwinkle

{"title":"ADD1 460W Allele Associated With Cardiovascular Disease in Hypertensive Individuals","authors":"A. Morrison, M. Bray, A. Folsom, E. Boerwinkle","doi":"10.1161/01.HYP.0000019128.94483.3A","DOIUrl":"https://doi.org/10.1161/01.HYP.0000019128.94483.3A","url":null,"abstract":"High blood pressure is a predictor of cardiovascular disease. Hence, genes contributing to essential hypertension may play a role in the etiology of cardiovascular disease. For this reason, we examined the association between the &agr;-adducin (ADD1) G460W and G-protein &bgr;3 subunit (GNB3) 825C>T polymorphisms and the prevalence of peripheral arterial disease (PAD) and incidence of coronary heart disease (CHD) in non-Hispanic whites from the Atherosclerosis Risk in Communities (ARIC) Study. PAD prevalence was defined by an ankle-brachial index, ie, the ratio of ankle systolic blood pressure to brachial artery systolic blood pressure, of ≤0.90 for men and ≤0.85 for women. CHD incidence was determined by following the ARIC cohort for a median of 5.3 years for potential coronary events. Stratified random samples of the ARIC cohort (n=703 and n=684) were used, respectively, as the comparison groups for the PAD (n=144) and incident CHD (n=408) cases. The GNB3 825T allele and the ADD1 460W allele were not significantly associated with prevalence of PAD or incidence of CHD. However, a test of the interaction between hypertension status and the ADD1 G460W polymorphism indicated that further evaluation of the ADD1 polymorphism in only hypertensive individuals was warranted. The ADD1 460W allele was significantly associated with PAD (odds ratio [OR]: 2.61, 95% CI, 1.27–5.37, P =0.01) and CHD (hazard rate ratio [HRR]: 2.30, 95% CI, 1.20–4.42, P =0.01) in hypertensive individuals after adjustment for multiple cardiovascular disease risk factors. An interaction with hypertension in the association between the ADD1 G460W polymorphism and cardiovascular disease merits further testing in additional populations.","PeriodicalId":13233,"journal":{"name":"Hypertension: Journal of the American Heart Association","volume":"14 1","pages":"1053-1057"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77563765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 72

Fetal Uninephrectomy Leads to Postnatal Hypertension and Compromised Renal Function 胎儿肾切除术导致产后高血压和肾功能损害

Hypertension: Journal of the American Heart Association Pub Date : 2002-06-01 DOI: 10.1161/01.HYP.0000019131.77075.54

K. Moritz, E. Wintour, M. Dodic

{"title":"Fetal Uninephrectomy Leads to Postnatal Hypertension and Compromised Renal Function","authors":"K. Moritz, E. Wintour, M. Dodic","doi":"10.1161/01.HYP.0000019131.77075.54","DOIUrl":"https://doi.org/10.1161/01.HYP.0000019131.77075.54","url":null,"abstract":"It has been proposed that the number of nephrons an individual has may be inversely related to his or her blood pressure. In this study using female ovine fetuses, nephron number was reduced by performing a fetal uninephrectomy during the period of active nephrogenesis (100 days of gestation, term=150 days). Lambs were born at term and grew at a similar rate. At 5 months of age, ovaries were removed and the carotid artery exteriorized into a fold of skin. Blood pressure and renal function were studied at 6 and 12 months of age. At 6 months of age, uninephrectomized lambs had significantly higher mean arterial blood pressure than sham-operated lambs (89±2 versus 82±2 mm Hg, P <0.05) when measured over a 3-day period. Heart rate was not different between the groups. Urine flow rate was similar, but glomerular filtration rate was significantly lower in uninephrectomized animals (P <0.05). Urinary concentrations and excretion rates of sodium tended to be higher in uninephrectomized animals but were similar for chloride and potassium. There was no evidence of proteinuria in the uninephrectomized lambs. Similar differences were observed in blood pressure and renal function at 12 months of age. Plasma renin concentrations at this age were lower in the uninephrectomized lambs (P <0.05). An oral salt load for 10 days did not increase blood pressure significantly in either group at 12 months of age, nor were there differences in the responsiveness to graded doses of angiotensin II. These results suggest that formation of a low nephron number in utero, may result in elevated blood pressure and compromised renal function in later life.","PeriodicalId":13233,"journal":{"name":"Hypertension: Journal of the American Heart Association","volume":"112 1","pages":"1071-1076"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87680673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 96

Genomewide Linkage Scan of Resting Blood Pressure: HERITAGE Family Study 静息血压的全基因组连锁扫描:HERITAGE家族研究

Hypertension: Journal of the American Heart Association Pub Date : 2002-06-01 DOI: 10.1161/01.HYP.0000018911.46067.6E

T. Rice, T. Rankinen, Y. Chagnon, M. Province, L. Pérusse, A. Leon, J. Skinner, J. Wilmore, C. Bouchard, D. Rao

{"title":"Genomewide Linkage Scan of Resting Blood Pressure: HERITAGE Family Study","authors":"T. Rice, T. Rankinen, Y. Chagnon, M. Province, L. Pérusse, A. Leon, J. Skinner, J. Wilmore, C. Bouchard, D. Rao","doi":"10.1161/01.HYP.0000018911.46067.6E","DOIUrl":"https://doi.org/10.1161/01.HYP.0000018911.46067.6E","url":null,"abstract":"The purpose of this study was to search for genomic regions influencing resting systolic (SBP) and diastolic (DBP) blood pressure (BP) in sedentary families (baseline), and for resting BP responses (changes) resulting from a 20-week exercise training intervention (post-training–baseline) in the Health, Risk Factors, Exercise Training, and Genetics (HERITAGE) Family Study. A genome-wide scan was conducted on 317 black individuals from 114 families and 519 white individuals from 99 families using a multipoint variance-components linkage model and a panel of 509 markers. Promising results were primarily, but not exclusively, found in the black families. Linkage evidence (P <0.0023) with baseline BP replicated other studies within a 1-logarithm of odds (LOD) interval on 2p14, 3p26.3, and 12q21.33, and provided new evidence on 3q28, 11q21, and 19p12. Results for several known hypertension genes were less compelling. For response BP, results were not very strong, although markers on 13q11 were mildly suggestive (P <0.01). In conclusion, these HERITAGE data, in conjunction with results from previous genomewide scans, provide a basis for planning future investigations. The major areas warranting further study involve fine mapping to narrow down 3 regions on 2q, 3p, and 12q that may contain “novel” hypertension genes, additional typing of some biological candidate genes to determine whether they are the sources of these and other signals, multilocus investigations to understand how and to what extent some of these candidates may interact, and multivariate studies to characterize any pleiotropy.","PeriodicalId":13233,"journal":{"name":"Hypertension: Journal of the American Heart Association","volume":"45 1","pages":"1037-1043"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77268765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 101

Myocyte Redistribution of GRK2 and GRK5 in Hypertensive, Heart-Failure–Prone Rats 高血压、心力衰竭大鼠心肌细胞GRK2和GRK5的再分布

Hypertension: Journal of the American Heart Association Pub Date : 2002-06-01 DOI: 10.1161/01.HYP.0000019130.09167.3B

X. Yi, A. Gerdes, Faqian Li

{"title":"Myocyte Redistribution of GRK2 and GRK5 in Hypertensive, Heart-Failure–Prone Rats","authors":"X. Yi, A. Gerdes, Faqian Li","doi":"10.1161/01.HYP.0000019130.09167.3B","DOIUrl":"https://doi.org/10.1161/01.HYP.0000019130.09167.3B","url":null,"abstract":"G protein–coupled receptor kinases (GRKs) are known to be involved in the development of cardiac hypertrophy. Their exact role and subcellular distribution during cardiac hypertrophy and failure remain to be elucidated. We examined expression and subcellular distribution of GRK2 and GRK5 in the left ventricle of female spontaneously hypertensive heart failure (SHHF) rats at 6 months of age using Western blots and fluorescent confocal microscopy. GRK2 was expressed mainly in the Triton X-100 soluble fraction in the left ventricle with similar expression levels between SHHF and age-matched Wistar-Kyoto (WKY) rats. GRK2 had a striated pattern which colocalized with sarcomeric &agr;-actinin and G protein in both SHHF and WKY rat myocytes and specifically accumulated in the intercalated disks of myocytes from SHHF but not WKY rats. GRK5 was expressed in both the Triton X-100 soluble fraction and Triton X-100 insoluble fraction in the left ventricle with similar expression levels between SHHF and WKY rats. GRK5 distributed diffusely in the cytoplasm in both SHHF and WKY rat myocytes and specifically accumulated in the nucleus of myocytes from SHHF but not WKY rats. GRK5 colocalized with coilin, the major component of the nuclear substructure involved in RNA synthesis and processing. The results suggest different roles for GRK2 and GRK5 in G-protein signaling and RNA biogenesis. Subcellular redistribution of GRK2 and GRK5 may be involved in cardiac hypertrophy resulting from chronic hypertension.","PeriodicalId":13233,"journal":{"name":"Hypertension: Journal of the American Heart Association","volume":"29 1","pages":"1058-1063"},"PeriodicalIF":0.0,"publicationDate":"2002-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74350277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 87

Angiotensin II Type 2 Receptor Gene Transfer Downregulates Angiotensin II Type 1a Receptor in Vascular Smooth Muscle Cells 血管紧张素II 2型受体基因转移下调血管平滑肌细胞血管紧张素II 1a型受体

Hypertension: Journal of the American Heart Association Pub Date : 2002-05-01 DOI: 10.1161/01.HYP.0000016179.52601.B4

Xueqing Jin, N. Fukuda, Jin-zi Su, Yimu Lai, R. Suzuki, Y. Tahira, H. Takagi, Y. Ikeda, K. Kanmatsuse, H. Miyazaki

{"title":"Angiotensin II Type 2 Receptor Gene Transfer Downregulates Angiotensin II Type 1a Receptor in Vascular Smooth Muscle Cells","authors":"Xueqing Jin, N. Fukuda, Jin-zi Su, Yimu Lai, R. Suzuki, Y. Tahira, H. Takagi, Y. Ikeda, K. Kanmatsuse, H. Miyazaki","doi":"10.1161/01.HYP.0000016179.52601.B4","DOIUrl":"https://doi.org/10.1161/01.HYP.0000016179.52601.B4","url":null,"abstract":"Two distinct subtypes of angiotensin (Ang) II receptors, type 1 (AT1) and type 2 (AT2), have been identified. Vascular smooth muscle cells (VSMCs) usually express AT1 receptor. To elucidate the direct effects of the AT2 receptor on the AT1 receptor in VSMCs, we transfected AT2 receptor gene into cultured rat VSMCs. Overexpression of AT2 receptor significantly decreased expression of AT1a receptor at both the mRNA and protein levels in the presence and absence of Ang II in VSMCs. Overexpression of AT2 receptor increased expression of bradykinin and inducible NO in the presence and absence of Ang II in VSMCs. Bradykinin B2 receptor antagonist HOE–140 and NO synthase inhibitor N&ohgr;-nitro-l-arginine methyl ester (L-NAME) inhibited the decreases in AT1a receptor expression by the overexpression of AT2 receptor in VSMCs. l-Arginine augmented the decrease in AT1a receptor expression. Overexpression of AT2 receptor suppressed basal DNA synthesis and proliferation of VSMCs and abolished response of DNA synthesis to Ang II in VSMCs. Our results demonstrate that overexpression of the AT2 receptor downregulates AT1a receptor expression in rat VSMCs in a ligand-independent manner that is mediated by the bradykinin/NO pathway. Downregulation of AT1a receptor is a novel mechanism by which the AT2 receptor regulates growth and metabolism of VSMCs.","PeriodicalId":13233,"journal":{"name":"Hypertension: Journal of the American Heart Association","volume":"25 1","pages":"1021-1027"},"PeriodicalIF":0.0,"publicationDate":"2002-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78234542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 68