Myocyte Redistribution of GRK2 and GRK5 in Hypertensive, Heart-Failure–Prone Rats

X. Yi, A. Gerdes, Faqian Li
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引用次数: 87

Abstract

G protein–coupled receptor kinases (GRKs) are known to be involved in the development of cardiac hypertrophy. Their exact role and subcellular distribution during cardiac hypertrophy and failure remain to be elucidated. We examined expression and subcellular distribution of GRK2 and GRK5 in the left ventricle of female spontaneously hypertensive heart failure (SHHF) rats at 6 months of age using Western blots and fluorescent confocal microscopy. GRK2 was expressed mainly in the Triton X-100 soluble fraction in the left ventricle with similar expression levels between SHHF and age-matched Wistar-Kyoto (WKY) rats. GRK2 had a striated pattern which colocalized with sarcomeric &agr;-actinin and G protein in both SHHF and WKY rat myocytes and specifically accumulated in the intercalated disks of myocytes from SHHF but not WKY rats. GRK5 was expressed in both the Triton X-100 soluble fraction and Triton X-100 insoluble fraction in the left ventricle with similar expression levels between SHHF and WKY rats. GRK5 distributed diffusely in the cytoplasm in both SHHF and WKY rat myocytes and specifically accumulated in the nucleus of myocytes from SHHF but not WKY rats. GRK5 colocalized with coilin, the major component of the nuclear substructure involved in RNA synthesis and processing. The results suggest different roles for GRK2 and GRK5 in G-protein signaling and RNA biogenesis. Subcellular redistribution of GRK2 and GRK5 may be involved in cardiac hypertrophy resulting from chronic hypertension.
高血压、心力衰竭大鼠心肌细胞GRK2和GRK5的再分布
已知G蛋白偶联受体激酶(GRKs)参与心脏肥厚的发生。它们在心脏肥厚和衰竭中的确切作用和亚细胞分布仍有待阐明。采用Western blot和荧光共聚焦显微镜观察6月龄雌性自发性高血压心力衰竭(SHHF)大鼠左心室GRK2和GRK5的表达和亚细胞分布。GRK2主要表达于左心室Triton X-100可溶性部分,在SHHF和年龄匹配的Wistar-Kyoto (WKY)大鼠中表达水平相似。GRK2在SHHF和WKY大鼠的肌细胞中均呈纹状分布,与肌聚体&agr;-actin和G蛋白共定位,并特异性地积聚在SHHF大鼠而WKY大鼠肌细胞的插层盘中。GRK5在SHHF和WKY大鼠左心室Triton X-100可溶部分和Triton X-100不可溶部分均有表达,表达水平相近。GRK5在SHHF和WKY大鼠肌细胞的细胞质中弥漫性分布,在SHHF而WKY大鼠肌细胞的细胞核中特异性积累。GRK5与coilin共定位,coilin是参与RNA合成和加工的核亚结构的主要成分。结果提示GRK2和GRK5在g蛋白信号传导和RNA生物发生中具有不同的作用。GRK2和GRK5的亚细胞再分布可能参与慢性高血压引起的心脏肥厚。
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