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65th Annual meeting of the Genetics Society of Japan. Mishima, September 17-19, 1993. Abstracts. 第65届日本遗传学会年会。三岛，1993年9月17日至19日。摘要。
Idengaku zasshi Pub Date : 1993-10-01
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引用次数: 0
Complementation assay of primer protein: gene expression systems of plasmid vectors support the infection of suppressor sensitive mutant phages M2 and phi 29. 引物蛋白的互补实验:质粒载体的基因表达系统支持抑制敏感突变体M2和phi 29的感染。
Idengaku zasshi Pub Date : 1993-08-01 DOI: 10.1266/jjg.68.243
T Kishi, K Miura, K Matsumoto, H Hirokawa
{"title":"Complementation assay of primer protein: gene expression systems of plasmid vectors support the infection of suppressor sensitive mutant phages M2 and phi 29.","authors":"T Kishi,&nbsp;K Miura,&nbsp;K Matsumoto,&nbsp;H Hirokawa","doi":"10.1266/jjg.68.243","DOIUrl":"https://doi.org/10.1266/jjg.68.243","url":null,"abstract":"<p><p>Two different expression systems of genes of primer proteins (pE for phage M2, and p3 for phi 29) were constructed to study the protein primed DNA replication of Bacillus phages M2 and phi 29. In one system, expression of the genes was under the control of the inducible spac promoter, whereas in the other system, the expression was under the control of the constitutive promoter in plasmid pUB110. Complementation tests in vivo were performed between the primer proteins expressed by these systems and mutant phages having suppressor sensitive mutations in the genes of the primer proteins. The phages M2 susE and phi 29 sus3 were complemented by pE and p3 expressed by the systems, respectively. However, the complementation and apparent phage DNA synthesis were not detected in the combinations between susE of phage M2 and p3 of phage phi 29, and vice versa. Although pE and p3 proteins exhibited structurally and functionally similar characteristics, these proteins showed species specificity in the protein primed DNA replication of bacteriophages M2 and phi 29.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 4","pages":"243-55"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.243","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19279552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Analysis of mitochondrial DNA in two species of the bipectinata species complex of Drosophila. 果蝇双翅种复合体的两种线粒体DNA分析。
Idengaku zasshi Pub Date : 1993-08-01 DOI: 10.1266/jjg.68.257
J P Gupta, T Aotsuka, A Inaba, O Kitagawa
{"title":"Analysis of mitochondrial DNA in two species of the bipectinata species complex of Drosophila.","authors":"J P Gupta,&nbsp;T Aotsuka,&nbsp;A Inaba,&nbsp;O Kitagawa","doi":"10.1266/jjg.68.257","DOIUrl":"https://doi.org/10.1266/jjg.68.257","url":null,"abstract":"<p><p>Drosophila bipectinata and D. malerkotliana are members of the bipectinata species complex of the ananassae subgroup in the melanogaster species group of Drosophila. The mtDNA from 18 isofemale strains of these species was analyzed, using 13 restriction endonucleases. Altogether, eight mtDNA haplotypes were detected, of which the haplotype 1B was shared by the two species. Restriction cleavage map of mtDNA of these species was constructed. The net nucleotide substitutions per site calculated between these species was found to be 0.0002. This value appeared to be relatively much lower than expected at the interspecific level, even lower than that reported between two subspecies of Drosophila despite both being good species. This extreme closeness of their mtDNAs is discussed in the light of recent findings.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 4","pages":"257-64"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.257","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19279553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
A novel mutation involved in the mitotic checkpoint in the fission yeast Schizosaccharomyces pombe. 分裂酵母有丝分裂检查点的新突变。
Idengaku zasshi Pub Date : 1993-08-01 DOI: 10.1266/jjg.68.265
J Ishiguro, N Yamada
{"title":"A novel mutation involved in the mitotic checkpoint in the fission yeast Schizosaccharomyces pombe.","authors":"J Ishiguro,&nbsp;N Yamada","doi":"10.1266/jjg.68.265","DOIUrl":"https://doi.org/10.1266/jjg.68.265","url":null,"abstract":"<p><p>The cps8 mutation which confers supersensitivity to a spindle poison, Isopropyl N-3-chlorophenyl carbamate (CIPC), in the fission yeast Schizosaccharomyces pombe was investigated. The cps8 mutant accumulated enlarged multinucleate cells in the stationary phase under normal growth conditions. The mutant was highly lethal at 36.5 degrees C in a fresh growth medium but not in a saline solution where the cell cycle ceases quickly. Lethality at high temperature was significantly suppressed by cdc1 or nda2 mutation which blocks nuclear division, but not by hydroxyurea treatment or cdc22 mutation which blocks DNA synthesis. A cdc10 cps8 double mutant remained lethal to high temperature, suggesting this double mutant to bypass the requirement for cdc10+ indispensable for the cell cycle start in a wild-type cell. After being transferred to a fresh medium at 36.5 degrees C, the multinucleate cells rapidly divided with aberrant nuclear segregation. Thus, cps8 mutation allows cells to undergo mitosis without DNA replication at the restrictive temperature. The cps8 gene was mapped on the left arm of chromosome II closely linked to but distinct from cdc2 locus.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 4","pages":"265-76"},"PeriodicalIF":0.0,"publicationDate":"1993-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.265","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19280173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Classification and relationships of rice strains with AA genome by identification of transposable elements at nine loci. 水稻AA基因组9个座转座元件的分类与关系研究。
Idengaku zasshi Pub Date : 1993-06-01 DOI: 10.1266/jjg.68.205
K Mochizuki, H Ohtsubo, H Hirano, Y Sano, E Ohtsubo
{"title":"Classification and relationships of rice strains with AA genome by identification of transposable elements at nine loci.","authors":"K Mochizuki,&nbsp;H Ohtsubo,&nbsp;H Hirano,&nbsp;Y Sano,&nbsp;E Ohtsubo","doi":"10.1266/jjg.68.205","DOIUrl":"https://doi.org/10.1266/jjg.68.205","url":null,"abstract":"<p><p>We analyzed the presence of p-SINE1 members at five loci in the rice strains belonging to seven species with AA genome in the Oryza genus by the methods including polymerase chain reaction (PCR). Four p-SINE1 members (p-SINE1-r3, r4, r5 and r7) were present at the corresponding loci in all the strains examined. One member (p-SINE1-r6) was, however, not present at the corresponding locus in most of the African strains of O. glaberrima and O. barthii, but was in the other strains. The PCR-amplified fragments containing p-SINE1-r4 in many strains were found to be larger due to insertion of either one of two transposable elements, named Tnr2 and Ret1, within or near p-SINE1-r4, respectively: Tnr2 is 157 bp in length with terminal inverted repeat sequences of about 56 bp; Ret1 is only 13 bp in length with a T stretch at its end. Tnr2 was not present in the corresponding locus in all the strains belonging to O. sativa Japonica and in some strains of O. rufipogon and O. longistaminata, while Ret1 was present only in the two strains of O. longistaminata. These results and previous ones obtained from the analysis of the other two p-SINE1 members (p-SINE1-r1 and r2) in the Wx gene indicate that the elements, such as p-SINE1-r6, Tnr2, Ret1 and p-SINE1-r2, have been inserted into the respective loci during divergence of the rice species with AA genome. The patterns for the presence and absence of the transposable elements at the respective loci enabled us to classify the rice strains with AA genome into ten groups and to infer their relationships.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 3","pages":"205-17"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.205","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19240380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
Genetic polymorphism of prophenoloxidase A1 in Drosophila melanogaster. 黑腹果蝇酚氧化酶原A1的遗传多态性。
Idengaku zasshi Pub Date : 1993-06-01
N Asada, K Fujimoto, M Tanaka, E Ohnishi
{"title":"Genetic polymorphism of prophenoloxidase A1 in Drosophila melanogaster.","authors":"N Asada,&nbsp;K Fujimoto,&nbsp;M Tanaka,&nbsp;E Ohnishi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Electrophoretic variations of prophenoloxidase were obtained from natural populations of Drosophila melanogaster. A1, one of the 2 isoforms of the prophenoloxidases, is polymorphic: 10 fast-migrating and 2 slow-migrating types were found in polyacrylamide gel electrophoresis among 271 wild strains. The majority were the intermediate-migrating type. By use of these variants, A1 gene was mapped at 79.6 on the right arm of the second chromosome. By deletion mapping, its cytological position was located at 55A-B. In the electropherograms, hybrids between the types differing in mobility exhibited 3 bands, indicating that A1 is a dimeric protein. The gene for A1 is expressed through larval, pupal and adult stages.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 3","pages":"219-27"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19239708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mapping of the mouse Rar loci encoding retinoic acid receptors RAR alpha, RAR beta and RAR gamma. 编码视黄酸受体Rar α、Rar β和Rar γ的小鼠Rar位点的定位。
Idengaku zasshi Pub Date : 1993-06-01 DOI: 10.1266/jjg.68.175
M Taketo, C Hoopes, T A Howard, E Linney, M F Seldin
{"title":"Mapping of the mouse Rar loci encoding retinoic acid receptors RAR alpha, RAR beta and RAR gamma.","authors":"M Taketo,&nbsp;C Hoopes,&nbsp;T A Howard,&nbsp;E Linney,&nbsp;M F Seldin","doi":"10.1266/jjg.68.175","DOIUrl":"https://doi.org/10.1266/jjg.68.175","url":null,"abstract":"<p><p>Nuclear retinoic acid receptors RAR alpha, RAR beta and RAR gamma are transcription factors that bind all-trans retinoic acid as their ligand and mediate its action by activating particular set of genes that contain retinoic acid responsive elements in their promoter-enhancers. We have mapped genetic loci for these genes using restriction fragment length variants (RFLVs) in interspecific backcross mice. None of the Rar loci cosegregated with each other or with the new subclass of retinoid receptors, Rxr loci. Rara mapped to mChr 11, Rarb mapped to mChr 14, and Rarg mapped to mChr 15. The results are consistent with the previous reports and the human data in terms of syntenic homology between mouse and human chromosomes.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 3","pages":"175-84"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.175","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19240378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Mitosis of rotenone-induced endoreduplication in Chinese hamster cells. 鱼藤素诱导的中国仓鼠细胞内复制的有丝分裂。
Idengaku zasshi Pub Date : 1993-06-01 DOI: 10.1266/jjg.68.185
K Matsumoto, T Ohta
{"title":"Mitosis of rotenone-induced endoreduplication in Chinese hamster cells.","authors":"K Matsumoto,&nbsp;T Ohta","doi":"10.1266/jjg.68.185","DOIUrl":"https://doi.org/10.1266/jjg.68.185","url":null,"abstract":"<p><p>Endoreduplication was induced by rotenone with an extremely high frequency (approximately 90% of all the metaphases) in cultured Chinese hamster cells. Endoreduplicated cells were fixed without colchicine or hypotonic treatment, and chromosomal configurations were examined in various mitotic stages. The two sister chromosomes of each diplochromosome at late prophase were widely separated except the centromeric region, but they became gradually paired along the total length as the cell cycle progressed to metaphase. The anaphase cells underwent multipolar division, resulting in three or four aneuploid daughter cells. Indirect immunofluorescence staining using anti-beta tubulin antibody revealed tripolar or tetrapolar spindles and unusual equatorial plates.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 3","pages":"185-94"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19240379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
cDNAs encoding for storage proteins in the tubers of taro (Colocasia esculenta Schott). 芋头块茎中编码储存蛋白的cdna。
Idengaku zasshi Pub Date : 1993-06-01 DOI: 10.1266/jjg.68.229
M Hirai, K Nakamura, T Imai, T Sato
{"title":"cDNAs encoding for storage proteins in the tubers of taro (Colocasia esculenta Schott).","authors":"M Hirai,&nbsp;K Nakamura,&nbsp;T Imai,&nbsp;T Sato","doi":"10.1266/jjg.68.229","DOIUrl":"https://doi.org/10.1266/jjg.68.229","url":null,"abstract":"<p><p>Two major protein groups of taro (Colocasia esculenta) tuber were purified, and their antisera were used for the screening of the cDNA library constructed from poly(A)+ RNA of taro tuber. A cDNA clone obtained by screening with an anti-12 kD protein antiserum had an insert 1058 bp-long, and an open reading frame for a peptide of 268 amino acids. The analyses of the N-terminal amino acid sequence and in vitro translation product suggested that the protein was synthesized as a peptide with a molecular weight of 27 kD, and then processed into two mature peptides with a molecular weight of 12.5 and 13.9 kD and an extra peptide with a molecular weight of 0.6 kD. The cDNA clones obtained using the anti-25 kD protein antiserum were highly homologous with each other. One of them had an insert 958 bp-long and an open reading frame for a peptide with 209 amino acids. The amino acid sequence deduced from the nucleotide sequence of this clone indicated that the 25 kD proteins were homologous to the trypsin inhibitors of soybean and winged bean as well as sporamins, the storage proteins of sweet potato.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 3","pages":"229-36"},"PeriodicalIF":0.0,"publicationDate":"1993-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.229","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19239709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 32
Temperature-dependent selection in the transmission of mitochondrial DNA in Drosophila. 果蝇线粒体DNA传递中的温度依赖性选择。
Idengaku zasshi Pub Date : 1993-04-01 DOI: 10.1266/jjg.68.127
E T Matsuura, Y Niki, S I Chigusa
{"title":"Temperature-dependent selection in the transmission of mitochondrial DNA in Drosophila.","authors":"E T Matsuura,&nbsp;Y Niki,&nbsp;S I Chigusa","doi":"10.1266/jjg.68.127","DOIUrl":"https://doi.org/10.1266/jjg.68.127","url":null,"abstract":"<p><p>We previously reported a selective mode of mitochondrial DNA (mtDNA) transmission in mtDNA heteroplasmy that was induced artificially in Drosophila melanogaster; the transmission bias appeared to depend on the particular temperature at which heteroplasmic lines were maintained. Here we report investigations of the temperature-dependent mode of mtDNA transmission in heteroplasmic lines for intra- and interspecific combinations maintained separately at 22.5 degrees C, 25 degrees C and 29 degrees C for 20 generations. We have examined a selection model for mitochondrial transmission, similar to genetic selection in haploid organisms. Changes in the relative proportions of two types of mtDNA fit the expectations from the model well. The intensity of selection estimated as a selection coefficient depends on temperature. Temperature-sensitive processes thus appear to be involved in the transmission and maintenance of mitochondria.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 2","pages":"127-35"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.127","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19355480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
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