{"title":"Synthesis of free X duplications carrying a specific region of the centromeric heterochromatin in Drosophila melanogaster.","authors":"H S Park, M T Yamamoto","doi":"10.1266/jjg.68.83","DOIUrl":"https://doi.org/10.1266/jjg.68.83","url":null,"abstract":"<p><p>Free X duplication chromosomes of Drosophila melanogaster were synthesized by X-ray irradiating the In(1)scL8Lsc8R chromosome which has a deletion in the distal half of hA and the proximal half of hB of the centromeric heterochromatin. Fifty-nine duplications have been isolated and cytogenetically analyzed. They all carry wild-type allele of the yellow gene, y+, which should come from the distal tip of In(1)scL8Lsc8R. They appear to be telocentric and predominantly heterochromatic. Majority of the duplications, especially in the classes MEDIUM and LARGE, can pair with XYL.YS in the male meiosis, indicating that they carry male meiotic pairing site(s) that is known to be located exclusively in the X heterochromatin. Complementation test in the males, Df(1)svr, v/Dp, y+, demonstrates that most of the duplications in the classes MEDIUM and LARGE carry euchromatin enough to cover the deletion. The portion of the euchromatin should be of the very proximal region close to the irradiated X chromosome centromere.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 2","pages":"83-95"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.83","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19355481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A theoretical approach to chromosome banding pattern analysis.","authors":"H T Imai","doi":"10.1266/jjg.68.97","DOIUrl":"https://doi.org/10.1266/jjg.68.97","url":null,"abstract":"<p><p>Based on a schematic model of karyotype evolution, a new methodology for G-, R-, or Q-banding pattern analysis was investigated. Banding pattern analysis essentially depends on the unidirectional alteration and the randomness of the exchange sites of the AM-inversion. In karyotypes that evolved by AM-inversion and Robertsonian rearrangement, two matching patterns appear; (1) tandem and (2) complementary matching patterns. The former is characteristic of a single lineage sharing the same AM-inversions, and the latter appears in different lineages sharing different AM-inversions, by which it is theoretically possible to detect the ancestral karyotype and to reconstruct the karyotype phylogeny (cladogram). In contrast, the evolutionary pathway cannot always be perceived if karyotypes evolve only by Robertsonian rearrangement. The tandem matching pattern does not always mean tandem fusion, but can be interpreted as 'tandem fission' by a combination of AM-inversion and centric fission. Tandem fusion and MM-inversion often cause entangled matching patterns, and thus they interfere with banding pattern analysis. Some methodological problems inherent in the conventional banding pattern analysis are highlighted, and suggested that such problems can be minimized by using the karyograph method. The methodology of banding pattern analysis proposed in the present paper will be applicable for matching the chromosome map of genetic markers among different species.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 2","pages":"97-118"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.97","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19355482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"RNA editing of rapeseed mitochondrial atp9 transcripts: RNA editing changes four amino acids, but termination codon is already encoded by genomic sequence.","authors":"H Handa","doi":"10.1266/jjg.68.47","DOIUrl":"https://doi.org/10.1266/jjg.68.47","url":null,"abstract":"<p><p>The gene encoding subunit 9 of Fo-ATPase of rapeseed mitochondria has been isolated. The complete genomic DNA sequence and cDNA sequence corresponding to the atp9 gene transcript have been determined by a method involving cDNA synthesis, using specific oligonucleotides as primers, followed by PCR amplification, cloning and sequencing of the amplification products. In comparison of cDNA sequences to genomic one, four modifications, C-to-U conversions, have been found. When compared with RNA editing patterns of atp9 transcripts among plant mitochondria, that of rapeseed atp9 transcript is more simple; there are only four editing sites on the coding region, and its termination codon is already encoded by genomic sequence.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 1","pages":"47-54"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.47","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19306002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ty1-copia group retrotransposons as ubiquitous components of plant genomes.","authors":"H Hirochika, R Hirochika","doi":"10.1266/jjg.68.35","DOIUrl":"https://doi.org/10.1266/jjg.68.35","url":null,"abstract":"<p><p>Ty1-copia group retrotransposons were searched for in 35 plant species by amplification of the reverse transcriptase coding region using the polymerase chain reaction. Sequences of the expected size were amplified from all of these plant species, including a liverwort, a horsetail, a bracken, gymnosperms and angiosperms. Sequences of 72 clones from 17 species were determined, all of which showed clear homology to the reverse transcriptase sequence of Ty1-copia type retrotransposons. More than half of the sequences carried stop codons or frame shifts. Twenty three new retrotransposon sequences with no interruption by these mutations were revealed. The mechanisms of the evolution of retrotransposons and accumulation of mutations were discussed.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 1","pages":"35-46"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19376973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K Kimura, T Okumura, O Ninaki, M G Kidwell, K Suzuki
{"title":"Transposable elements in commercially useful insects: I. Southern hybridization study of silkworms and honeybees using Drosophila probes.","authors":"K Kimura, T Okumura, O Ninaki, M G Kidwell, K Suzuki","doi":"10.1266/jjg.68.63","DOIUrl":"https://doi.org/10.1266/jjg.68.63","url":null,"abstract":"<p><p>As a first step in surveying transposable elements in silkworms and honeybees, hybridization analyses were carried out using 16 known families of Drosophila transposable elements as probes. jockey and G were the only transposable elements that hybridized with genomic DNA of either honeybees or silkworms under the conditions of this study. jockey hybridized with genomic DNA of both European honeybees (Apis mellifera) and silkworms (Bombyx mori and Antheraea yamamai) and showed significant bands in Southern blots. Banding patterns were highly polymorphic. jockey did not, however, hybridize with any strains of the Asian honeybee (A. cerana). G elements showed a faint signal with the Asian honeybee, but not with any other insects tested. The results suggest that, even though it has some limitations, this approach can be used in practice as a first preliminary step in surveys for the presence of transposable elements in organisms which do not have good genetic information.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"68 1","pages":"63-71"},"PeriodicalIF":0.0,"publicationDate":"1993-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.68.63","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19376974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quantitative genetic study on sexual difference in emigration behavior of Drosophila melanogaster in a natural population.","authors":"K Mikasi","doi":"10.1266/jjg.67.463","DOIUrl":"https://doi.org/10.1266/jjg.67.463","url":null,"abstract":"<p><p>A quantitative genetic analysis was conducted on emigration response behavior using 140 second chromosome lines of Drosophila melanogaster. Fourteen sets of 5 x 5 partial diallel cross experiments were made in the parental generation. The emigration activity per batch of 50 male and 50 female F1 progeny was scored with Sakai's population system. Sexual difference did not appear in the emigration activity in these experiments. A significant genotype x sex x set interaction was detected. The genetic variance components of emigration activity differed between sexes: In males, additive genetic variance of emigration activity was 0.0497 +/- 0.0092 and dominance variance, 0.0018 +/- 0.0046; in females, additive, 0.0373 +/- 0.0076 and dominance, 0.0169 +/- 0.0044. Additive genetic correlation between sexes for the emigration activity was 0.685 +/- 0.150, deviating significantly from unity. These results suggested that the genes affecting emigration activity would operate differently between sexes of D. melanogaster in natural populations.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"67 6","pages":"463-72"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.67.463","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12479665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A novel cDNA clone for acid invertase in tomato fruit.","authors":"A Ohyama, M Hirai, S Nishimura","doi":"10.1266/jjg.67.491","DOIUrl":"https://doi.org/10.1266/jjg.67.491","url":null,"abstract":"<p><p>The sequence of a novel cDNA clone, Aiv-1, for tomato acid invertase was similar to that of TIV1 (Klann et al., 1992) for the enzyme except for a unique intron-like insertion. It is considered that Aiv-1 is derived from either an alternatively spliced mRNA for an isozyme or a pre-mRNA of TIV1.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"67 6","pages":"491-2"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.67.491","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12479666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Variance component analysis of bristle characters in local populations of Drosophila melanogaster.","authors":"M Ichinose, H Tachida, T Mukai","doi":"10.1266/jjg.67.449","DOIUrl":"https://doi.org/10.1266/jjg.67.449","url":null,"abstract":"<p><p>The genetic variabilities of sternopleural and abdominal bristle numbers existing in local natural populations were assessed. Using second chromosome lines of Drosophila melanogaster extracted from three natural populations in Japan (the Ishigakijima, Ogasawara and Aomori populations), experiments were conducted to estimate the components of genetic variances, additive and dominance variances. The following results were obtained: For both sternopleural and abdominal bristle numbers, the additive genetic variances (sigma 2A) were much larger than the dominance variances (sigma 2D) especially in the southern populations. For example, in the Ishigakijima population, for females sternopleural bristle numbers of the inversion-free chromosome group, the additive and dominance variances were estimated to be 1.255 +/- 0.2034 and 0.0552 +/- 0.0180, respectively. The magnitudes of the estimates of additive genetic variances were nearly equal from north to south. By comparing the additive genetic variances of the inversion-free chromosome group with those of the In(2L)t-carrying chromosome group, it was inferred that sufficient number of generations to achieve the equilibrium state has not passed since the introduction of a single or a small number of the In(2L)t-carrying chromosomes to the Ishigakijima population.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"67 6","pages":"449-61"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.67.449","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12479664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Action site of the lethal Ay gene in the mouse embryo.","authors":"Y Saijoh, T Takeuchi","doi":"10.1266/jjg.67.357","DOIUrl":"https://doi.org/10.1266/jjg.67.357","url":null,"abstract":"<p><p>We investigated the lethal effect of Ay gene in embryos at the preimplantation stage in vitro. First, the development until the blastocyst stage and the division of individual cells from 8-cell stage embryos were examined. No difference in development was detected between embryos from the experimental cross (Ay/a x Ay/a) and those from the control cross (a/a x a/a). Therefore, it seems that the abnormality of the Ay/Ay embryo does not appear until blastocyst formation in vitro. We subsequently examined the hatching from zona pellucida of the blastocysts. The hatching ratio of the embryos from the experimental cross was significantly lower than that of the control crosses (Ay/a x a/a, a/a x a/a: p < 0.05). Our observation indicates that deficiency of the Ay/Ay embryo can be detected in vitro at hatching. In order to elucidate the mechanism of the gene action of the Ay, we attempted to rescue the lethal embryos from decreased hatching ratio in vitro. When dbcAMP at the concentration of 1 mM was added to the culture medium, the hatching ratio of blastocysts from the experimental cross increased until the level of those from the control crosses. Since this result indicates that the cAMP content in Ay homozygote seemed to be lower than those in a/a and Ay/a, the cAMP content in individual blastocyst was quantified. It is found that Ay homozygosity was associated with lower level of cAMP. When adenylate cyclase was activated by forskolin and cholera toxin, the hatching ratio was increased. These results seem to suggest that Ay homozygote embryos possess a defect in signal transduction system mediated by adenylate cyclase during hatching.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"67 5","pages":"357-70"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.67.357","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12511159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Induction of endoreduplication in double minutes of the human neuroblastoma cells and the replication pattern.","authors":"S Takayama, Y Hirano, H Hiramatsu","doi":"10.1266/jjg.67.397","DOIUrl":"https://doi.org/10.1266/jjg.67.397","url":null,"abstract":"<p><p>Induction of endoreduplication (ERD) using Hoechst 33258 as well as colcemid was carried out in cultured neuroblastoma (NB) line cells. In these endoreduplicated cells, the majority of double minutes (DMs) appeared to take a diplochromosome like configuration to form a cluster consisting of four minute elements, assuming a complex DM. Sister chromatid differential staining (SCD) using 5-bromo-2'-deoxyuridine (BrdUrd) revealed the non-random distribution of the stained chromatids among four chromatids composing each diplochromosome, suggesting the occurrence of so-called \"outside replication\" of DNA strands during the process of ERD. The same pattern of differential staining was also found in the quadruple minutes of each endoreduplicated DM. Since DMs are acentric, the present results suggest that centromeres do not play any essential role in the formation of diplochromosomes observed in the conventional cytologic preparations and that centromeres are probably not responsible for the phenomenon of the \"outside replication\" of DNA strands.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"67 5","pages":"397-403"},"PeriodicalIF":0.0,"publicationDate":"1992-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.67.397","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12471708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}