Idengaku zasshi最新文献_第10页

Molecular cloning and characterization of gravity specific cDNA in rice (Oryza sativa L.) suspension callus. 水稻(Oryza sativa L.)悬浮愈伤组织重力特异性cDNA的分子克隆与鉴定。

Idengaku zasshi Pub Date : 1992-08-01 DOI: 10.1266/jjg.67.335

S T Kwon, S Kikuchi, K Oono

{"title":"Molecular cloning and characterization of gravity specific cDNA in rice (Oryza sativa L.) suspension callus.","authors":"S T Kwon, S Kikuchi, K Oono","doi":"10.1266/jjg.67.335","DOIUrl":"https://doi.org/10.1266/jjg.67.335","url":null,"abstract":"Rice (Oryza sativa L. var. Nipponbare) suspension callus was exposed to gravity stress at 450,000 g for 2 hours, after which poly(A)+RNA was isolated and a cDNA library was constructed. Three different gravity specific cDNAs, namely, GSC 128, GSC 233 and GSC 381 of 0.67, 0.60 and 0.68 kilobase pairs and transcripts of 1.9, 1.6 and 2.0 kb, respectively, were isolated by differential screening and Northern hybridization. The maximum level of transcript was achieved after 4 hours of exposure to gravity at 450,000 g for GSC 128, 2 hours for GSC 233 and 8 hours for GSC 381 followed by a gradual decrease to undetectable levels with the extension of gravitation time. Callus (GSC 128), shoot and callus (GSC 381) and root and callus (GSC 233) specific expression of transcripts was identified. Although the protection of callus by treatment with ABA, kinetin and sucrose extended the period of expression of mRNA in suspension callus after gravity exposure, the expression of gravity-inducible mRNA was exclusively regulated by the degree of callus viability or survival after the stress. In addition, we demonstrated that the level of GSC 381 transcript was markedly increased by exposing the cell to periodical gravity stress, suggesting that this mRNA is expressed and translated into special proteins which are closely related to the survival of the cell against gravity stress. The sequence of GSC 233 and GSC 381, consisting of 417 and 531 base pairs of the longest open reading frames, encode polypeptides with calculated molecular weights of 15.29 and 19.47 kDa, respectively. A sequence homology search against a data bank revealed that GSC 233 and GSC 381 differed from other stress inducible genes in terms of the coding sequence and expression characteristics.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"67 4","pages":"335-48"},"PeriodicalIF":0.0,"publicationDate":"1992-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.67.335","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12537300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Insertional mutagenesis in Drosophila. II. P element mediated transformation of Drosophila yakuba. 果蝇的插入突变。2P元素介导的果蝇转化。

Idengaku zasshi Pub Date : 1992-08-01 DOI: 10.1266/jjg.67.291

S Togashi, R Ueda, M Takahisa, M Mikuni, K Kondo, T Miyake

引用次数: 0

Compensatory changes in silver-stainability of nucleolar organizer regions in mice. 小鼠核仁组织区银染色的代偿性变化。

Idengaku zasshi Pub Date : 1992-06-01 DOI: 10.1266/jjg.67.217

H Suzuki, S Sakurai, M Nishimura, R Kominami, K Moriwaki

引用次数: 17

Amino acid substitution of proteins coded for in mitochondrial DNA during mammalian evolution. 哺乳动物进化过程中线粒体DNA编码蛋白质的氨基酸替代。

Idengaku zasshi Pub Date : 1992-06-01 DOI: 10.1266/jjg.67.187

J Adachi, M Hasegawa

引用次数: 37

Cytological mapping of Om mutants of Drosophila ananassae. 果蝇Om突变体的细胞学定位。

Idengaku zasshi Pub Date : 1992-06-01 DOI: 10.1266/jjg.67.259

H Matsubayashi, M Matsuda, Y Tomimura, M Shibata, Y N Tobari

引用次数: 10

Isolation and characterization of mutants supersensitive to the spindle poison, isopropyl N-3-chlorophenyl carbamate (CIPC) in the fission yeast Schizosaccharomyces pombe. 分裂酵母对纺锤体毒素异丙基n -3-氯苯基氨基甲酸酯(CIPC)超敏感突变体的分离与鉴定。

Idengaku zasshi Pub Date : 1992-04-01 DOI: 10.1266/jjg.67.97

J Ishiguro, Y Uhara

{"title":"Isolation and characterization of mutants supersensitive to the spindle poison, isopropyl N-3-chlorophenyl carbamate (CIPC) in the fission yeast Schizosaccharomyces pombe.","authors":"J Ishiguro, Y Uhara","doi":"10.1266/jjg.67.97","DOIUrl":"https://doi.org/10.1266/jjg.67.97","url":null,"abstract":"Mutants supersensitive to the spindle poison, Isopropyl N-3-chlorophenyl carbamate (CIPC) of the fission yeast Schizosaccharomyces pombe were isolated and characterized genetically. Fourteen different recessive loci were assigned for the mutation (donated as cps1 to cps14) and two, cps1 and cps3, were mapped precisely on the chromosomes. Nine mutant strains were also supersensitive to phenothiazine derivatives, inhibitors of calcium-binding protein calmodulin. Four of nine strains were incapable of growing in the presence of 10 microM calcium ionophore A23187, at which the drug had no effect on cell growth in other strains. Fluorescence microscopy using the DAPI and Calcofluor staining methods showed two strains out of four to be defective in normal cell division; most stationary-phase cells of the cps6 mutant were seen to be bi- or tetra-nucleate, being partitioned with one or three septa, respectively. In the other mutant (cps8), enlarged cells were unequally partitioned with multisepta, and each compartment contained several daughter nuclei. The septa appeared aberrant in position within the cell, and situated diagonally but not vertically along the long cell axis.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"67 2","pages":"97-109"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.67.97","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12693664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Variations in chloroplast proteins and nucleotide sequences of three chloroplast genes in Triticum and Aegilops. 小麦和豌豆叶绿体蛋白和三个叶绿体基因核苷酸序列的差异。

Idengaku zasshi Pub Date : 1992-04-01 DOI: 10.1266/jjg.67.111

T M Ikeda, T Terachi, K Tsunewaki

{"title":"Variations in chloroplast proteins and nucleotide sequences of three chloroplast genes in Triticum and Aegilops.","authors":"T M Ikeda, T Terachi, K Tsunewaki","doi":"10.1266/jjg.67.111","DOIUrl":"https://doi.org/10.1266/jjg.67.111","url":null,"abstract":"Two alloplasmic wheat lines having the same common wheat nucleus but the cytoplasms of Aegilops crassa and Ae. columnaris together with the corresponding normal line (control) were used in the two-dimensional gel electrophoresis of soluble and thylakoid membrane proteins of the chloroplast. Three chloroplast polypeptides: the Rubisco large subunit, the beta subunit of ATP synthase, and an unidentified 31 kDa protein, differed in the common wheat and two Aegilops cytoplasms. Three chloroplast genes, atpB, atpE and trnM, that respectively encode the beta and epsilon subunits of ATP synthase and tRNA(met), were sequenced. The atpB gene differed by two synonymous base substitutions, whereas the other two genes were identical in the two Aegilops cytoplasms. From the predicted amino acid sequences, the beta subunits of the ATP synthase in the Aegilops cytoplasms were assumed to have three amino acid substitutions: Ala by Val, Asp- by Ala, and Gln by Lys+, in contrast to the cytoplasm of common wheat. This accounts for the difference in pI values found for the common wheat and Aegilops cytoplasms. The two base substitutions for the atpE genes of common wheat and the Aegilops cytoplasms were synonymous. The differences detected in the genes encoding the two subunits of ATP synthase do not appear to be ascribable to the differences in phenotypic effects for the common wheat and Aegilops cytoplasms. The base substitution rate of the atpB-atpE-trnM gene cluster was similar to that of the rbcL gene. From the rate for the atpB gene alone, evolutionary divergence of the wheat-Aegilops complex is assumed to have begun ca. 3.0 x 10(6) years ago, as compared to ca. 8.0 x 10(6) years ago for the divergence of the wheat-Aegilops complex and barley.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"67 2","pages":"111-23"},"PeriodicalIF":0.0,"publicationDate":"1992-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.67.111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12559106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Suppression of alpha-amylase gene expression by antisense oligodeoxynucleotide in barley cultured aleurone layers. 反义寡脱氧核苷酸抑制大麦糊粉层α -淀粉酶基因表达的研究。

Idengaku zasshi Pub Date : 1992-04-01 DOI: 10.1266/jjg.67.147

N Tsutsumi, K Kanayama, S Tano

引用次数: 18

Spontaneous mutations affecting glycerol-3-phosphate dehydrogenase enzyme activity in Drosophila melanogaster. 影响黑腹果蝇甘油-3-磷酸脱氢酶活性的自发突变。

Idengaku zasshi Pub Date : 1992-04-01 DOI: 10.1266/jjg.67.125

A Koga, K Harada, S Kusakabe, T Mukai

引用次数: 4

Molecular structure of the human alcohol dehydrogenase 3 gene. 人酒精脱氢酶3基因的分子结构。

Idengaku zasshi Pub Date : 1992-04-01 DOI: 10.1266/jjg.67.167

S Yokoyama, Y Matsuo, S Rajasekharan, R Yokoyama

引用次数: 4