Idengaku zasshi最新文献_第10页

Molecular cloning and nucleotide sequencing of the Arthrobacter dextranase gene and its expression in Escherichia coli and Streptococcus sanguis. 节杆菌葡聚糖酶基因的分子克隆、核苷酸测序及其在大肠杆菌和血链球菌中的表达。

Idengaku zasshi Pub Date : 1991-04-01 DOI: 10.1266/jjg.66.173

M Okushima, D Sugino, Y Kouno, S Nakano, J Miyahara, H Toda, S Kubo, A Matsushiro

{"title":"Molecular cloning and nucleotide sequencing of the Arthrobacter dextranase gene and its expression in Escherichia coli and Streptococcus sanguis.","authors":"M Okushima, D Sugino, Y Kouno, S Nakano, J Miyahara, H Toda, S Kubo, A Matsushiro","doi":"10.1266/jjg.66.173","DOIUrl":"https://doi.org/10.1266/jjg.66.173","url":null,"abstract":"A bacterial strain, which assimilated dextran and water-insoluble glucan produced by Streptococcus mutans, was isolated from soil. The bacterium produced and secreted potent dextranase activity, which was identified as Arthrobacter sp. and named CB-8. The dextranase was purified and some enzymatic properties were characterized. The enzyme efficiently decomposed the water-insoluble glucan as well as dextran. A gene library from the bacteria was constructed with Escherichia coli, using plasmid pUC19, and clones producing dextranase activity were selected. Based on the result of nucleotide sequencing analysis, it was deduced that the dextranase was synthesized in CB-8 cells as a polypeptide precursor consisting of 640 amino acid residues, including 49 N-terminal amino acid residues which could be regarded as a signal peptide. In the E. coli transformant, the dextranase activity was detected mostly in the periplasmic space. The gene for the dextranase was introduced into Streptococcus sanguis, using an E. coli-S. sanguis shuttle vector that contained the promoter sequence of a gene for glucosyltransferase derived from a strain of S. mutans. The active dextranase was also expressed and accumulated in S. sanguis cells.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"66 2","pages":"173-87"},"PeriodicalIF":0.0,"publicationDate":"1991-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.66.173","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13021921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Selective transmission of mitochondrial DNA in heteroplasmic lines for intra- and interspecific combinations in Drosophila melanogaster. 黑腹果蝇异质系线粒体DNA在种内和种间组合中的选择性传递。

Idengaku zasshi Pub Date : 1991-04-01 DOI: 10.1266/jjg.66.197

E T Matsuura, Y Niki, S I Chigusa

引用次数: 20

[Factors for activation of the replication origin of the E. coli chromosome]. [激活大肠杆菌染色体复制起源的因素]。

Idengaku zasshi Pub Date : 1991-02-01 DOI: 10.1266/jjg.66.85

T Asai

引用次数: 2

Suppression of the cr-1 mutation in Neurospora crassa. 粗神经孢子虫cr-1突变的抑制。

Idengaku zasshi Pub Date : 1991-02-01 DOI: 10.1266/jjg.66.77

S Kore-eda, T Murayama, I Uno

引用次数: 12

On the robertsonian polymorphism found in the Japanese raccoon dog (Nyctereutes procyonoides viverrinus). 日本貉(Nyctereutes procyonoides viverrinus)的robertsonian多态性研究。

Idengaku zasshi Pub Date : 1991-02-01 DOI: 10.1266/jjg.66.1

M Y Wada, H T Imai

引用次数: 19

Chromosomal rearrangement In(2)TY and linkage maps of the second chromosome of Drosophila virilis. 雄性果蝇染色体重排In(2)TY和第二染色体连锁图谱。

Idengaku zasshi Pub Date : 1991-02-01 DOI: 10.1266/jjg.66.49

K Tsuno, O Yamaguchi

引用次数: 3

Distorted segregation of the esterase isozyme genotypes in barley (Hordeum vulgare L.). 大麦酯酶同工酶基因型的扭曲分离。

Idengaku zasshi Pub Date : 1990-12-01 DOI: 10.1266/jjg.65.411

T Konishi, K Abe, S Matsuura, Y Yano

引用次数: 29

Fitness and its components in Drosophila melanogaster. 黑腹果蝇的适合度及其组成成分。

Idengaku zasshi Pub Date : 1990-12-01 DOI: 10.1266/jjg.65.417

T Tanaka, T Yamazaki

引用次数: 10

Transient expression of chimeric CAT genes injected into early embryos of the domesticated silkworm Bombyx mori. 家蚕早期胚胎嵌合CAT基因的瞬时表达。

Idengaku zasshi Pub Date : 1990-12-01 DOI: 10.1266/jjg.65.401

T Tamura, T Kanda, S Takiya, K Okano, H Maekawa

{"title":"Transient expression of chimeric CAT genes injected into early embryos of the domesticated silkworm Bombyx mori.","authors":"T Tamura, T Kanda, S Takiya, K Okano, H Maekawa","doi":"10.1266/jjg.65.401","DOIUrl":"https://doi.org/10.1266/jjg.65.401","url":null,"abstract":"In order to establish a transient expression system for genes introduced into early embryos of the silkworm, Bombyx mori, we tested various promoters ligated with CAT reporter genes. The embryos into which we injected supercoiled plasmid DNA of pFb(-860/+10)CAT containing the Bombyx fibroin promoter region ligated to the CAT gene showed a reasonably high CAT activity beginning around 30 h after oviposition. This high activity was observed only when the plasmid was injected before termination of the early nuclear cleavage stage, which was about 8 h after oviposition, but not after this stage. This means that the expression of injected DNA is closely related to the presence of cleavage nuclei in early embryos. Promoters originating from insect genes, like the Bombyx sericin-1 gene, Drosophila hsp70 and Drosophila copia LTR, functioned as strong promoters in the embryos. On the contrary, promoters from mammalian virus genes, such as the SV40 early and Moloney murine leukemia virus LTR genes, functioned as weak promoters. Moreover, linearized DNAs showed no or weak activity of expression in embryos. From these results, we conclude that the silkworm embryo transient expression system is a useful tool for studying the mechanism of regulation of insect genes.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"65 6","pages":"401-10"},"PeriodicalIF":0.0,"publicationDate":"1990-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.65.401","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13243979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

An E. coli promoter that is sensitive to visible light. 一种对可见光敏感的大肠杆菌启动子。

Idengaku zasshi Pub Date : 1990-10-01 DOI: 10.1266/jjg.65.381

K Nakahigashi, Y Komine, M Watanabe, H Inokuchi

引用次数: 2