Idengaku zasshi最新文献_第4页

Analysis of the tyrosinase gene of the Japanese pond frog, Rana nigromaculata: cloning and nucleotide sequence of the genomic DNA containing the tyrosinase gene and its flanking regions. 日本黑斑蛙(Rana nigromaculata)酪氨酸酶基因的分析:包含酪氨酸酶基因及其侧翼区基因组DNA的克隆和核苷酸序列。

Idengaku zasshi Pub Date : 1995-02-01 DOI: 10.1266/jjg.70.79

I Miura, H Okumoto, K Makino, A Nakata, M Nishioka

{"title":"Analysis of the tyrosinase gene of the Japanese pond frog, Rana nigromaculata: cloning and nucleotide sequence of the genomic DNA containing the tyrosinase gene and its flanking regions.","authors":"I Miura, H Okumoto, K Makino, A Nakata, M Nishioka","doi":"10.1266/jjg.70.79","DOIUrl":"https://doi.org/10.1266/jjg.70.79","url":null,"abstract":"Three genomic DNA fragments containing the tyrosinase-encoding gene (TYR) of the Japanese pond frog, Rana nigromaculata, were cloned. The first, clone I, was isolated from a genomic library of sperm DNA using the mouse TYR cDNA as the probe and contained a DNA segment similar to exon 4 of the mouse TYR gene. Subsequently, the TYR cDNA was isolated by screening a frog embryo cDNA library using clone I as the probe. Two clones that contain genomic DNA of the TYR gene were isolated also from a blood cell DNA library using the frog TYR cDNA as the probe. Comparison of the nucleotide (nt) sequences of the genomic clone II DNA and the cDNA revealed that clone II contained a 3,140-bp DNA fragment consisting of the 5'-flanking region, the first exon, and a part of the first intron. The region upstream of the coding region contained the characteristic sequences for regulatory elements, including TATA- and CAAT-motifs, and also a pigment cell-specific promoter element, which is shared by the promoter regions of the vertebrate TYR genes. A 764-bp segment containing an upstream 748-bp non-coding region and 16-bp coding region was functional for expression of the promoter-less cat gene on a plasmid in the transiently transformed albino frog melanophore. The genomic clone III contained the 3'-untranslated region of the mRNA and its 3'-flanking region. Thus, the cDNA plus genomic DNA fragments isolated here cover the entire TYR gene and its flanking regions.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"70 1","pages":"79-92"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.70.79","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18772198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Relationships between morphometric and mitochondrial DNA differentiation in laboratory strains of musk shrews (Suncus murinus). 麝鼠(Suncus murinus)实验室品系形态计量学与线粒体DNA分化的关系。

Idengaku zasshi Pub Date : 1995-02-01 DOI: 10.1266/jjg.70.57

A Ishikawa, T Yamagata, T Namikawa

{"title":"Relationships between morphometric and mitochondrial DNA differentiation in laboratory strains of musk shrews (Suncus murinus).","authors":"A Ishikawa, T Yamagata, T Namikawa","doi":"10.1266/jjg.70.57","DOIUrl":"https://doi.org/10.1266/jjg.70.57","url":null,"abstract":"Morphometric differentiation among six strains of musk shrews (Suncus murinus) originating in Bangladesh (BAN), Sri Lanka (SRI), and four Japanese areas, Nagasaki (NAG), Okinawa Island (OKI), Tokunoshima Island (TKU), and Taramajima Island (TR), was examined by use of skull and external measurements. These data were compared with mitochondrial DNA (mtDNA) differentiation previously reported. Significant sexual dimorphism was observed in all morphometric characters of the six strains, except for two characters in the TR strain. The six strains were clearly grouped by principal component analysis into three body-size types: large BAN shrews, intermediate SRI shrews, and small Japanese shrews. By canonical discriminant analysis, the NAG strain was further distinct from the other three Japanese strains despite their apparent similarities in external morphology, and was characterized by having the most unusual shape in the six strains. No individuals were misclassified as to their geographic origin for both sexes of the six strains. The morphometric differentiation pattern based on only the first canonical variate, extracting an overall size factor, was concordant with the mtDNA differentiation pattern (rss = 0.944, P < 0.001 in males and rss = 0.930, P < 0.001 in females). In contrast, the morphometric differentiation pattern estimated from the second to the fifth canonical variates, representing shape factors, was apparently discordant with the mtDNA differentiation pattern (rss = 0.467, P > 0.05 in both sexes). It was previously reported that a partial premating reproductive isolation mechanism is caused by the difference in body size between mating pairs. Thus, body size may be a factor useful for understanding the mechanisms of population differentiation in this species.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"70 1","pages":"57-74"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.70.57","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18772195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

DNA sequence changes in mutations in the tonB gene on the chromosome of Escherichia coli K12: insertion elements dominate the spontaneous spectra. 大肠杆菌K12染色体上tonB基因突变的DNA序列变化:插入元件主导自发光谱。

Idengaku zasshi Pub Date : 1995-02-01 DOI: 10.1266/jjg.70.35

K Kitamura, Y Torii, C Matsuoka, K Yamamoto

{"title":"DNA sequence changes in mutations in the tonB gene on the chromosome of Escherichia coli K12: insertion elements dominate the spontaneous spectra.","authors":"K Kitamura, Y Torii, C Matsuoka, K Yamamoto","doi":"10.1266/jjg.70.35","DOIUrl":"https://doi.org/10.1266/jjg.70.35","url":null,"abstract":"To obtain insight into the nature and mechanisms of spontaneous mutations, Escherichia coli K12 strain TM31 was constructed to determine, by DNA sequencing, the mutational spectrum of the tonB gene on the chromosome. We inserted the chloramphenicol resistant gene 1.6 kb upstream of the tonB gene, thus making it possible to retrieve the mutated tonB gene from the chromosome by shotgun cloning using a drug-resistant marker. The spontaneous mutation frequency in the tonB gene, which was judged by its colicin B-resistant phenotype, is 3-10 x 10(-7). Spontaneous mutations were dominated by large insertions that are identified by DNA sequencing to be IS elements; IS1 dominated, but IS2, IS5, and IS10 were also obtained. In uvrA- strain, transposition of both IS10-R and IS10-L are equally increased, suggesting the interaction of the UvrA protein and IS10 transposition. The base substitutions are the second largest group of mutations, among which G:C-->A:T transition is predominant. Deletions also contribute significantly in wild type with regard to DNA repair and uvrA- strains, but not recA- strain, suggesting that the RecA protein is involved to some extent in deletion formation. Endpoints of these deletions do not always correlate with the presence of repeated sequences, indicating the absence of homologous recombination for deletion formation.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"70 1","pages":"35-46"},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.70.35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18772193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Genetic variation estimated in three Shorea species by the RAPD analysis. 利用RAPD分析估计了三种雪梨属植物的遗传变异。

Idengaku zasshi Pub Date : 1994-12-01 DOI: 10.1266/jjg.69.713

K Harada, A Kinoshita, N A Shukor, H Tachida, T Yamazaki

引用次数: 15

Genetic characterization of Drosophila RBP-J kappa (suppressor of hairless) as a neurogenic gene in adult PNS development. 果蝇RBP-J kappa(无毛抑制基因)在成年PNS发育中的遗传特征。

Idengaku zasshi Pub Date : 1994-12-01 DOI: 10.1266/jjg.69.701

T Furukawa, K Kimura, Y Kobayakawa, K Tamura, M Kawaichi, T Tanimura, T Honjo

引用次数: 8

Cloning of the cDNA and genomic DNA that correspond to the recA-like gene of Drosophila melanogaster. 黑腹果蝇reca样基因cDNA及基因组DNA的克隆。

Idengaku zasshi Pub Date : 1994-12-01 DOI: 10.1266/jjg.69.663

E Akaboshi, Y Inoue, H Ryo

引用次数: 27

Molecular analysis of cis-regulatory sequences of the alpha-amylase gene in D. melanogaster: a short 5'-flanking region of Amy distal gene is required for full expression of Amy proximal gene. 黑腹蝶α -淀粉酶基因顺式调控序列的分子分析:Amy远端基因的5'侧短区域是Amy近端基因完全表达所必需的。

Idengaku zasshi Pub Date : 1994-12-01 DOI: 10.1266/jjg.69.619

J Choi, T Yamazaki

{"title":"Molecular analysis of cis-regulatory sequences of the alpha-amylase gene in D. melanogaster: a short 5'-flanking region of Amy distal gene is required for full expression of Amy proximal gene.","authors":"J Choi, T Yamazaki","doi":"10.1266/jjg.69.619","DOIUrl":"https://doi.org/10.1266/jjg.69.619","url":null,"abstract":"The structural locus for alpha-amylase (AMY) in Drosophila melanogaster is duplicated and divergently transcribed. These two genes are designated as Amy-p and Amy-d, respectively. We searched for the cis-acting regulatory elements for full expression of the duplicated Amy-p and Amy-d loci, by injecting plasmid constructs containing sequences from the Amy locus into preblastoderm embryos of an AMY-null strain and measuring exogenous AMY activity produced in transformed host larvae (i.e., the transient expression assay). Relative activities of endogenous amylase isozymes, AMY-1 and AMY-3, in extracts of AMY1,3 larvae of a Canton-S are almost the same. However, three independently isolated Amy-p1 constructs with only the 5' upstream regions of Amy-p1 expressed a very low AMY-1 activity. Two other Amy-p1 constructs with the 5' upstream region of Amy-d3 in addition to that of Amy-p1 produced a high activity. Thus, the 5' upstream region of Amy-d3 is necessary for full expression of Amy-p1. In order to locate cis-regulatory elements within the 5' region of Amy-d3, a series of hybrid constructs including this region were tested to locate them. Our results clearly show that the cis-acting regulatory sequences required for full expression of Amy-p1 are located between the base pairs at -304 and -372 upstream of Amy-d3 gene. In other words, only a short region located upstream of Amy-d3 was found to be necessary and sufficient for the full expression of Amy-p1 in addition to its promoter. This region seems also necessary for the expression of Amy-d3.","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 6","pages":"619-35"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.69.619","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18856989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Placental glucose dehydrogenase polymorphism in Koreans. 韩国人胎盘葡萄糖脱氢酶多态性。

Idengaku zasshi Pub Date : 1994-12-01 DOI: 10.1266/jjg.69.657

Y J Kim, S G Paik, H Y Park

引用次数: 0

Molecular cloning and characterization of a fission yeast gene responsible for supersensitivity to the spindle poison, isopropyl N-3-chlorophenyl carbamate. 对纺锤体毒素异丙基n -3-氯苯基氨基甲酸酯超敏感的裂变酵母基因的克隆和鉴定。

Idengaku zasshi Pub Date : 1994-12-01 DOI: 10.1266/jjg.69.671

J Ishiguro, Y Uhara, K Kawahara

引用次数: 6

Chromosomal localization of human, rat, and mouse protein phosphatase type 1 beta catalytic subunit genes (PPP1CB) by fluorescence in situ hybridization. 荧光原位杂交技术定位人、大鼠和小鼠蛋白磷酸酶1型β催化亚基基因(PPP1CB)

Idengaku zasshi Pub Date : 1994-12-01 DOI: 10.1266/jjg.69.697

M Saadat, Y Kakinoki, Y Mizuno, K Kikuchi, M C Yoshida

引用次数: 7