K Harada, A Kinoshita, N A Shukor, H Tachida, T Yamazaki
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引用次数: 15
Abstract
Three species of Shorea (S. leprosula, S. acuminata and S. cursitii) were collected from a natural forest reserve of Malaysia and analyzed for genetic variation using the technique of random amplification of polymorphic DNA (RAPD) by the polymerase chain reaction (PCR). The average number of nucleotide substitutions was estimated. The nucleotide diversities within species were very similar and larger than those found in Drosophila melanogaster. The nucleotide divergences between these species are about 1.5 times the nucleotide diversities within the species, indicating that these species diverged from a common ancestor relatively recently.
采用聚合酶链反应(PCR)随机扩增多态性DNA (RAPD)技术，对马来西亚一天然森林保护区的3种Shorea (S. lemacsula, S. acuminata和S. cursitii)进行遗传变异分析。估计核苷酸取代的平均数目。种内核苷酸的多样性与黑腹果蝇非常相似，而且比黑腹果蝇更大。这些物种之间的核苷酸差异大约是物种内部核苷酸差异的1.5倍，表明这些物种从一个共同的祖先分化出来的时间相对较近。
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