{"title":"Eutherian phylogeny as inferred from mitochondrial DNA sequence data.","authors":"Y Cao, J Adachi, M Hasegawa","doi":"10.1266/jjg.69.455","DOIUrl":"https://doi.org/10.1266/jjg.69.455","url":null,"abstract":"<p><p>The phylogenetic relationships among Primates, Artiodactyla, Cetacea, Carnivora, and Rodentia were estimated from the amino acid sequences of proteins encoded by the mitochondrial genomes, for which entire nucleotide sequence data are available, using Marsupialia, Aves, and Amphibia as outgroups. The overall evidence of the maximum likelihood, as well as maximum parsimony, analyses strongly suggests that Rodentia is an outgroup to the other four eutherian orders, and that Cetacea and Artiodactyla form a clade with Carnivora as a sister taxon, consistently with the molecular phylogenetic studies of previous authors. However, analyses of individual proteins do not necessarily conform to this conclusion, and some of the proteins reject the putatively correct tree with nearly 5% significance. Furthermore, the 12S mitochondrial ribosomal RNA sequences do not give the putatively correct tree irrespective of the alignments and of the phylogenetic methods, although the tree is not rejected with a statistical significance. The 16S ribosomal RNA sequences give the putatively correct tree with our sequence alignment when the ML method is used, but the result depends on the alignment and on the choice of outgroup species. These results illustrate the limitation of the ribosomal RNA data alone in phylogenetic inference, and suggest that we must analyze as many genes as possible and synthesize the results to draw a reliable conclusion.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 5","pages":"455-72"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.69.455","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18994056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evolution of pathogenic viruses with special reference to the rates of synonymous and nonsynonymous substitutions.","authors":"T Gojobori, Y Yamaguchi, K Ikeo, M Mizokami","doi":"10.1266/jjg.69.481","DOIUrl":"https://doi.org/10.1266/jjg.69.481","url":null,"abstract":"<p><p>For pathogenic viruses such as human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), human influenza A virus, and human T-cell leukemia virus type I (HTLV-I), the evolutionary features were briefly reviewed with special reference to the rates of synonymous and nonsynonymous substitutions. In particular, these rates were discussed in connection with the neutral theory of molecular evolution. It was common to all the five pathogenic viruses that the rate of synonymous substitution was higher than that of nonsynonymous substitution particularly when the entire gene regions were compared between different isolates. This suggests that the viral proteins are quite conservative to functional and structural changes even though most of these viral genomes are evolving at a speed extraordinarily higher than their host genomes. Thus, this feature is consistent with the neutral theory. However, it is also pointed out that positive selection may be operating on some specific sites such as antigenic sites in order for the pathogenic viruses to escape from the host immune system.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 5","pages":"481-8"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.69.481","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18994058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evolution of glutamine synthetase genes is in accordance with the neutral theory of molecular evolution.","authors":"Y Tateno","doi":"10.1266/jjg.69.489","DOIUrl":"https://doi.org/10.1266/jjg.69.489","url":null,"abstract":"<p><p>Evolution of glutamine synthetase gene is discussed on the results of DNA sequence analysis of the gene. Thirty DNA sequences of various organisms spanning from prokaryotes to eukaryotes were collected from the DNA data banks and translated first, they were aligned next, then evolutionary distances were computed, and molecular phylogeny was finally estimated. The results of the alignment reveal that functionally important regions of glutamine synthetase have been evolutionarily more conserved than the remaining regions. The evolutionary distances computed also show that the rate of synonymous substitution is higher than that of nonsynonymous substitution. These are well in accordance with the neutral theory of molecular evolution. Besides, the molecular phylogeny obtained shows that the origin of glutamine synthetase gene is much earlier than the divergence between eukaryotes and prokaryotes, suggesting that the gene is one of the oldest genes functioning now.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 5","pages":"489-502"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.69.489","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18994059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A possible link between molecular evolution and tissue evolution demonstrated by tissue specific genes.","authors":"T Miyata, K Kuma, N Iwabe, N Nikoh","doi":"10.1266/jjg.69.473","DOIUrl":"https://doi.org/10.1266/jjg.69.473","url":null,"abstract":"<p><p>In this paper, we reviewed our recent works on a possible link between molecular evolution and tissue evolution. The evolutionary rates of genes that are expressed tissue specifically were shown to differ widely to one another, depending on tissues: Brain specific genes evolve with significantly slower rate than immune specific genes. The tissue dependence of molecular evolutionary rate strongly suggests the presence of functional constraints against molecular changes from tissue level. A molecular phylogenetic analysis of tissue specific isoforms that are identical to one another in function, but differ only in tissue distribution revealed frequent gene duplications and rapid accumulations of amino acid substitutions during the early evolution of chordates, where rapid evolution at the tissue or organ levels is thought to have occurred. On the basis of functional constraints, a possible explanation for the correlation between evolution at the two levels was presented.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 5","pages":"473-80"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.69.473","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18994057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mammalian phylogeny inferred from multiple protein data.","authors":"K Kuma, T Miyata","doi":"10.1266/jjg.69.555","DOIUrl":"https://doi.org/10.1266/jjg.69.555","url":null,"abstract":"<p><p>On the basis of multiple protein data, the phylogenetic relationships among the major clades of eutherian mammals, Primates, Cetacea, Artiodactyla, Carnivora, Lagomorpha, Myomorpha, and Caviomorpha, were analyzed by the maximum likelihood (ML) method, together with the maximum parsimony (MP) method and the neighbor joining (NJ) method. Using nineteen protein data, we first reexamined the hypothesis of rodent polyphyly proposed by Graur et al. (1991) based on the four taxon system, consisting of human, mouse (or rat), guinea-pig and an outgroup. The ML analysis does not support the rodent polyphyly, but strongly favors the traditional view of rodent monophyly representing the Myomorpha-Caviomorpha association. In the second analysis, the phylogenetic position of Lagomorpha were reexamined using three sets of multiple protein data, 13 mitochondrial DNA-coded proteins from rodents, rabbit, human, seals, bovine, whales and opossum, 25 protein data from human, rabbit and rodents and an outgroup, and 20 protein data from human, rabbit, bovine (or pig) and rodents. Our analysis favors the closer affinity of Lagomorpha to Primates than to Rodentia. The branching sequence of seven mammalian groups revealed by the present analyses is as follows: Myomorpha-Caviomorpha clade (Rodentia), rabbit, Primates, Carnivora, and Artiodactyla-Cetacea clade. Judging from the branch lengths measured by the numbers of synonymous substitutions, a series of divergence of these mammals is likely to be bush-like. The rapid rate of rodent evolution was reexamined in the light of the new phylogeny.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 5","pages":"555-66"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.69.555","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18994061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A polymorphic microsatellite marker from the tropical tree Dryobalanops lanceolata (Dipterocarpaceae).","authors":"R Terauchi","doi":"10.1266/jjg.69.567","DOIUrl":"https://doi.org/10.1266/jjg.69.567","url":null,"abstract":"<p><p>Di-nucleotide microsatellites were isolated from a genomic library of a tropical tree species, Dryobalanops lanceolata, in Sarawak, for the purpose of using them as hypervariable genetic markers to study the pollen-mediated gene flow. Among 1600 recombinant clones, in total 20 clones gave positive signals when hybridized with oligonucleotides with the three different repeat motifs, GT, CA and CT. Estimations of abundance of (GT)n/(CA)n and (GA)n/(CT)n dinucleotide repeats in D. lanceolata genome revealed to be one in every 84 kb and 80 kb, respectively. Among six sequenced microsatellite loci, one was selected to synthesize PCR primers to amplify the microsatellite. PCR product size of the locus was variable among different individuals, which is attributed to the different number of di-nucleotide repeats. The same microsatellite genotype was detected in the trunk and canopy of a single large tree, indicating the utility of trunk tissue as the source of DNA for the population genetic study of tropical tree species, the canopy of which is usually difficult to approach.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 5","pages":"567-76"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.69.567","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18992507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Centromeric repetitive sequences in Arabidopsis thaliana.","authors":"M Murata, Y Ogura, F Motoyoshi","doi":"10.1266/jjg.69.361","DOIUrl":"https://doi.org/10.1266/jjg.69.361","url":null,"abstract":"<p><p>Two highly repetitive DNA sequences have been cloned from Arabidopsis thaliana, ecotype Columbia, and were characterized by molecular and cytological analyses. These two sequences belong to the same repeat family with 180-bp basic unit, being tandemly organized in clusters. Pulsed field gel electrophoresis showed that this repeat sequence family forms at least seven clusters from ca. 100 to 1200 kb in length and ca. 3500 kb in total. Fluorescent in situ hybridization to somatic metaphase cells with the monomeric repeat unit as a probe clearly revealed that this repeat family is located at the centromeric regions of all chromosomes. It was also shown that this repetitive sequence is closely associated with limited parts of heterochromatic blocks on the centromeric regions which are visible distinctly at meiotic prophase from leptotene to diakinesis. Furthermore, this sequence hybridized preferentially to both polar sides of five bivalent chromosomes at the first metaphase. These results suggest that the repetitive sequences of this family were derived from the regions very close to the centromeres or on the centromeres themselves.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 4","pages":"361-70"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.69.361","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18551520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Allodiploid nature of Allium wakegi Araki revealed by genomic in situ hybridization and localization of 5S and 18S rDNAs.","authors":"M Hizume","doi":"10.1266/jjg.69.407","DOIUrl":"https://doi.org/10.1266/jjg.69.407","url":null,"abstract":"<p><p>Allium wakegi Araki could be originated from cross hybridization between close relatives of a form of A. cepa and A. fistulosum. Chromosomes of each parental haploid set were identified in the chromosome complement of A. wakegi by genomic in situ hybridization using probes of the total, parental genomic DNAs of A. cepa and A. fistulosum, respectively. The results of GISH suggested significant differentiation of genomic DNAs between these closely related species. Allium cepa had two 5S rDNA loci at the interstitial regions of the short arms of small chromosome pair (the 7th pair) and A. fistulosum had one locus at the interstitial region of the homologous short arms. Allium wakegi had two chromosomes carrying one and two 5S rDNA loci which appeared to correspond to those of A. fistulosum and A. cepa, respectively. Chromosomes carrying 18S rDNA loci originated from those of both A. cepa and A. fistulosum were also observed in the chromosome complement of A. wakegi.</p>","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 4","pages":"407-15"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1266/jjg.69.407","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18941383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"New DNA data collected and processed at DNA Data Bank of Japan.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":13120,"journal":{"name":"Idengaku zasshi","volume":"69 4","pages":"439-53"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18941382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}