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Micropropagation and Acclimatization of Monstera deliciosa Liebm. ‘Thai Constellation’ Monstera deliciosa Liebm.泰国星座
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-19 DOI: 10.3390/horticulturae10010001
Yifan Jing, David G. Beleski, Wagner Vendrame
{"title":"Micropropagation and Acclimatization of Monstera deliciosa Liebm. ‘Thai Constellation’","authors":"Yifan Jing, David G. Beleski, Wagner Vendrame","doi":"10.3390/horticulturae10010001","DOIUrl":"https://doi.org/10.3390/horticulturae10010001","url":null,"abstract":"Monstera deliciosa Liebm. ‘Thai Constellation’ is a variegated variety of M. deliciosa belonging to the family Araceae, which has become a new favorite in the foliage plant market. However, limited studies exist on its propagation, and growers have difficulties in achieving large-scale production. This study aimed at developing an efficient protocol for the micropropagation of M. deliciosa using SETIS™ temporary immersion bioreactors. Furthermore, we aimed at evaluating the role of a novel biostimulant (IQ Forte) in the acclimatization of M. deliciosa. Significant differences were observed among the different treatments, showing higher multiplication rates under TIS conditions as compared to the semi-solid control. Adjusting immersion parameters also showed benefits in improving multiplication rates. The novel biostimulant (IQ Forte) did not provide significant gains in growth of M. deliciosa ‘Thai Constellation’ during acclimatization.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":"124 42","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138958929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Response of Microbial Recovery Rate to Straw Return after Calcium Cyanamide Soil Disinfection 氰氨化钙土壤消毒后微生物恢复率对秸秆还田的响应
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-19 DOI: 10.3390/horticulturae10010002
Xuewen Xie, Lida Chen, Yanxia Shi, A. Chai, Tengfei Fan, Baoju Li, Lei Li
{"title":"Response of Microbial Recovery Rate to Straw Return after Calcium Cyanamide Soil Disinfection","authors":"Xuewen Xie, Lida Chen, Yanxia Shi, A. Chai, Tengfei Fan, Baoju Li, Lei Li","doi":"10.3390/horticulturae10010002","DOIUrl":"https://doi.org/10.3390/horticulturae10010002","url":null,"abstract":"At present, returning vegetable straw in situ is an effective measure to solve environmental pollution and improve soil properties. However, the direct return of straw to the field can reduce the release rate of soil organic matter and cause serious soilborne diseases. The combined application of calcium cyanamide (CaCN2) and straw can solve this problem. The objective of this study was to determine the effect of CaCN2 combined with pepper straw return on cucumber yield, soil physicochemical properties, and soil microbial communities during 2020 to 2021 in Shandong Province, China. The treatments were designed as follows: (1) calcium cyanamide soil disinfection, CC; (2) fresh pepper straw return, LJ; (3) fresh pepper straw return combined with calcium cyanamide disinfection, LJ+CC; and (4) natural soil without straw return treatment, CK. Compared with CK, the LJ+CC treatment significantly improved cucumber production by 20%. The cultivable microbial community in the soil was temporarily inhibited during soil fumigation treatment, and the cultivable bacterial and actinomycete communities in the soil return to their initial levels after the film was removed (harvest period). The numbers of culturable bacteria and actinomycetes in the soil in the LJ+CC treatment were 4.68 × 107 CFU/g and 5.17 × 107 CFU/g, respectively, higher than those in the soil in the CC treatment. The contents of TN and OM in the LJ+CC treatment increased by 13.1% and 13.5%, respectively, compared with that in LJ. Therefore, the LJ+CC treatment enhanced soil fertility and cucumber yields. CaCN2 can promote straw decomposition and straw can promote soil microbial recovery, and their combined application is considered a feasible and sustainable technique for utilizing vegetable residues in the greenhouse. The combination of returning pepper straw to the field and calcium cyanamide technology achieves a win-win situation of resource circulation and economic circulation by converting agricultural waste into fertilizer before being put into production. Based on this, it is recommended that the straw returning technology receives strong policy support, stimulates researchers to explore the feasibility of different vegetable straw returning to the field, promotes the implementation of this technology achievement, and leverages the environmental benefits of the application of straw returning technology.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":" 31","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138961400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Molecular Mechanism of Relatively Low-Temperature-Induced Broccoli Flower Bud Differentiation Revealed by Transcriptomic Profiling 转录组分析揭示相对低温诱导西兰花花芽分化的分子机制
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-18 DOI: 10.3390/horticulturae9121353
Wenchen Chai, Xia He, Boyue Wen, Yajie Jiang, Zixuan Zhang, Rui Bai, Xinling Zhang, Jin Xu, L. Hou, Meilan Li, Jing Zhang
{"title":"The Molecular Mechanism of Relatively Low-Temperature-Induced Broccoli Flower Bud Differentiation Revealed by Transcriptomic Profiling","authors":"Wenchen Chai, Xia He, Boyue Wen, Yajie Jiang, Zixuan Zhang, Rui Bai, Xinling Zhang, Jin Xu, L. Hou, Meilan Li, Jing Zhang","doi":"10.3390/horticulturae9121353","DOIUrl":"https://doi.org/10.3390/horticulturae9121353","url":null,"abstract":"Broccoli (Brassica oleracea L. var. italica) has a large, edible green flower head, which is one of its critical economic traits. A prerequisite of broccoli flower head formation is flower bud differentiation (FBD). Low-temperature treatment is an effective way to induce FBD in broccoli. However, the molecular mechanism underlying low-temperature-induced broccoli FBD remains largely unclear. In this study, using broccoli cultivar Zhongqing 10 as the experimental material, we investigated the effects of low-temperature treatment on FBD by comparing the plants grown at low temperatures (17 °C/9 °C, 16 h/8 h) with the control plants grown under normal temperature conditions (25 °C/17 °C, 16 h/8 h). After 15 days of different temperature treatments, the flower buds of the plants growing under the low-temperature condition started to differentiate. However, the control plants remained in the vegetative growth stage, indicating that low temperature successfully induced flower bud formation. Subsequently, a global transcriptomic analysis was conducted to detect the differentially expressed genes (DEGs) during low-temperature-induced FBD in broccoli. A total of 14 DEGs in five phytohormone signaling pathways, 42 DEGs in nine transcription factor families, and 16 DEGs associated with the floral development pathways were identified. More DEGs were present in the auxin signaling pathway than in other phytohormone signaling pathways, which indicated that the auxin signaling pathway played a critical role in modulating low-temperature-induced FBD in broccoli. Furthermore, four TF classes, including bZIP, GCM domain factors, MADS-box factors, and C2H2 zinc finger factors, possessed enriched motifs, indicating that their closely related DETFs ABI5, HY5L, WRKY11, WRKY15, WRKY22, SOC1, AGL8, FLC, SPL8, and SPL15 may be directly involved in the transcription regulation of broccoli FBD. This study provides an important basis for further investigation of the molecular regulatory mechanism of broccoli flower development under low temperatures.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":" 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138994776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
UPLC–MS/MS and Gene Expression Research to Distinguish the Colour Differences of Rhododendron liliiflorum H. Lév 通过 UPLC-MS/MS 和基因表达研究区分杜鹃花的颜色差异
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-18 DOI: 10.3390/horticulturae9121351
Jin-Ying Dai, Xinglin Wang, Xingpan Meng, Xu Zhang, Qihang Zhou, Zhengdong Zhang, Ximin Zhang, Yin Yi, Lunxian Liu, Tie Shen
{"title":"UPLC–MS/MS and Gene Expression Research to Distinguish the Colour Differences of Rhododendron liliiflorum H. Lév","authors":"Jin-Ying Dai, Xinglin Wang, Xingpan Meng, Xu Zhang, Qihang Zhou, Zhengdong Zhang, Ximin Zhang, Yin Yi, Lunxian Liu, Tie Shen","doi":"10.3390/horticulturae9121351","DOIUrl":"https://doi.org/10.3390/horticulturae9121351","url":null,"abstract":"Among ornamental plants, the colour of the petals is an important feature. However, the reason for the colour differences of Rhododendron liliiflorum remains unclear. To reveal the differences in the colour of R. liliiflorum, high-efficiency liquid chromatographic collar (UPLC–MS/MS) technology was used to study the yellow and white parts of R. liliiflorum. A total of 1187 metabolites were identified in R. liliiflorum petals, including 339 flavonoid metabolites. Seventy-eight types of flavonoids in these metabolites were found in the yellow and white parts of R. liliiflorum petals, along with 11 other significantly enriched substances. Combining gene expression-related data with differential metabolite data demonstrated effects of enrichment in the flavanonols (fustin), flavonols (epiafzelechin and afzelechin), and flavanones (pinocembrin) of flavonoid biosynthesis; glyccitin, 6″-O-malonylgenistin, and 6-hydroxydaidzein of isoflavonoid biosynthesis; and anthocyanin biosynthesis of malvidin-3-O-galactoside (primulin), delphinidin-3-O-rutinoside, cyanidin-3-O-glucoside (kuromanin), and cyanidin-3-O-rutinoside (keracyanin), which are potentially the contributing factors responsible for the differences in petal colour in R. liliiflorum. This study establishes a connection between the differential metabolites underlying the color differences in the petals of R. liliiflorum and the gene expression in R. liliiflorum. This will provide a foundation for subsequent research on the regulation of flower color in R. liliiflorum and have profound implications for horticultural applications of R. liliiflorum.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":"313 ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139173375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Paulownia spp.: A Bibliometric Trend Analysis of a Global Multi-Use Tree 泡桐属全球多种用途树木的文献计量趋势分析
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-18 DOI: 10.3390/horticulturae9121352
Linda Lugli, Giustino Mezzalira, Maurizio Lambardi, Huaxin Zhang, N. La Porta
{"title":"Paulownia spp.: A Bibliometric Trend Analysis of a Global Multi-Use Tree","authors":"Linda Lugli, Giustino Mezzalira, Maurizio Lambardi, Huaxin Zhang, N. La Porta","doi":"10.3390/horticulturae9121352","DOIUrl":"https://doi.org/10.3390/horticulturae9121352","url":null,"abstract":"The research on Paulownia spp. has increased in the last twenty years thanks to the growing interest in the application modalities of this plant in various sectors such as wood, phytoremediation, environmental protection, paper, biofuel, chemistry and medicine. For the first time, this study analyzed the papers present in the Web of Science Core Collection on “Paulownia” to obtain a set of characteristics in the work carried out from 1971 to 2021. This analysis selected and took into account 820 articles and provided evidence of the scientific production of authors, institutions, and countries. This work showed that the most studied species was Paulownia tomentosa, followed by P. fortunei and P. elongate. The JCR category and research area with the most publications was plant science, with 20.4% of the total. The papers were published in 460 journals and in a book series. The journals with the most publications were Bioresources, Advanced Material Research, Agroforestry Systems, Journal of Wood Science and Industrial Crops and Products. The institutions with the most prolific affiliation with the field of Paulownia spp. research were Henan University, the US Department of Agriculture, Belgrade University, the Chinese Academy, and Georgia University. Finally, the 3842 keywords were divided into nine different clusters and the trends of interest in the last fifteen years were highlighted.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":"36 5","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening and Selection for Herbicide Tolerance among Diverse Tomato Germplasms 在不同番茄种质中进行除草剂耐受性筛选和选育
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-18 DOI: 10.3390/horticulturae9121354
Gourav Sharma, Swati Shrestha, T. Tseng, Sanju Shrestha
{"title":"Screening and Selection for Herbicide Tolerance among Diverse Tomato Germplasms","authors":"Gourav Sharma, Swati Shrestha, T. Tseng, Sanju Shrestha","doi":"10.3390/horticulturae9121354","DOIUrl":"https://doi.org/10.3390/horticulturae9121354","url":null,"abstract":"Solanum lycopersicum, the domesticated species of tomato, is produced and consumed globally. It is one of the most economically important vegetable crops worldwide. In the commercial production of tomatoes, tomatoes are extremely sensitive to herbicide drifts from row crops in the vicinity. Injury to tomatoes from auxin herbicides and glyphosate can occur at rates as low as 0.01×. This results in a substantial yield reduction, and at high drift rates, plants may not show signs of recovery. With the new herbicide-resistant crop technologies on the market, which include 2,4-D and dicamba-resistant crops, there is an increase in the usage of these herbicides, causing more serious drift problems. There is a diverse germplasm of tomatoes that includes wild relatives which are tolerant to numerous biotic and abiotic stresses. Herbicide/chemical stress is an abiotic stress, and wild tomato accessions may have a natural tolerance to herbicides and other abiotic stresses. In the current study, diverse tomato genotypes consisting of 110 accessions representing numerous species, Solanum habrochaites, S. cheesmaniae, S. pimpinellifolium, S. chilense, S. lycopersicum, S. pimpinellifolium, S. galapagense, S. chimelewskii, S. corneliomulleri, S. neorickii, and S. lycopersicoides, were used for screening drift rate herbicide tolerance. The herbicides tested included simulated drift rates of 2,4-D, dicamba, glyphosate, quinclorac, aminopyralid, aminocyclopyrachlor, and picloram. The visual injury rating of each accession for each herbicide treatment was taken 7, 14, 21, and 28 days after treatment (DAT) on a scale of 0–100%. Numerous accessions were found to have minimal injury (less than 20%) for each of the herbicides tested; nine accessions were found for both 2,4-D and glyphosate, eleven for dicamba, five for quinclorac, eight for aminocyclopyrachlor and two for both aminopyralid and picloram at 28 DAT. The identification of genotypes with a higher herbicide tolerance will provide valuable genetic resources for the development of elite tomato varieties that can resist herbicide injury and produce competitive yields.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":"87 ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139174330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Photoperiod and Gibberellin on the Bolting and Flowering of Non-Heading Chinese Cabbage 光周期和赤霉素对不抽薹大白菜茎叶和开花的影响
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-18 DOI: 10.3390/horticulturae9121349
Shuping Liu, Junyang Lu, Jun Tian, Ping Cao, Shuhao Li, Haicui Ge, Mingxuan Han, Fenglin Zhong
{"title":"Effect of Photoperiod and Gibberellin on the Bolting and Flowering of Non-Heading Chinese Cabbage","authors":"Shuping Liu, Junyang Lu, Jun Tian, Ping Cao, Shuhao Li, Haicui Ge, Mingxuan Han, Fenglin Zhong","doi":"10.3390/horticulturae9121349","DOIUrl":"https://doi.org/10.3390/horticulturae9121349","url":null,"abstract":"Non-heading Chinese cabbage (cabbage) is an essential green leafy vegetable, and bolting and flowering are necessary for reproduction. However, further research is needed to study the effect of photoperiod on the bolting and flowering of cabbage, particularly on the development of the stem. In this study, we performed phenotypic analysis and measured endogenous gibberellin levels in the cabbage. We carried out these experiments under four different photoperiodic treatments, 12 h (light)/12 h (dark), 14 h (light)/10 h (dark), 16 h (light)/8 h (dark), and 18 h (light)/6 h (dark). The results showed that the time of bolting and flowering gradually decreased with increasing light duration. The development of stems was optimal under the 16 h (light)/8 h (dark) photoperiod treatment, and the same result was obtained via cytological observation. In addition, the changes in the endogenous gibberellin3 (GA3) content under different photoperiodic treatments were consistent with the development of stems and peaked at 16 h (light)/8 h (dark). At the same time, qRT-PCR analysis showed that the relative expression of the key gibberellin synthase genes, BcGA3ox2 and BcGA20ox2, exhibited upregulation. When treated with exogenous GA3 and its synthesis inhibitor, paclobutrazol (PAC), exogenous gibberellins significantly promoted bolting; conversely, gibberellin inhibitors suppressed the bolting, flowering, and stem elongation of cabbage. Therefore, the photoperiod may regulate cabbage bolting by regulating endogenous GA3.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":"64 4","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138965280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Arbuscular Mycorrhizal Fungi, Especially Rhizophagus intraradices as a Biostimulant, Improve Plant Growth and Root Columbin Levels in Tinospora sagittata 丛枝菌根真菌,特别是作为生物刺激剂的根瘤菌(Rhizophagus intraradices),可改善虎耳草的植物生长和根部哥伦宾含量
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-18 DOI: 10.3390/horticulturae9121350
Lu-Lu Meng, Fu-Qi Xu, Ze-Zhi Zhang, M. Alqahtani, Manal A. Tashkandi, Qiang-Sheng Wu
{"title":"Arbuscular Mycorrhizal Fungi, Especially Rhizophagus intraradices as a Biostimulant, Improve Plant Growth and Root Columbin Levels in Tinospora sagittata","authors":"Lu-Lu Meng, Fu-Qi Xu, Ze-Zhi Zhang, M. Alqahtani, Manal A. Tashkandi, Qiang-Sheng Wu","doi":"10.3390/horticulturae9121350","DOIUrl":"https://doi.org/10.3390/horticulturae9121350","url":null,"abstract":"Tinospora sagittata is a columbin-rich medicinal plant, but its columbin levels are reduced under artificial cultivation conditions. The objective of this study was to analyze the effects of inoculations with Diversispora versiformis (Dv), Funneliformis mosseae (Fm), Rhizophagus intraradices (Ri), and mixed inoculation (Dv + Fm + Ri) (Mix) on growth performance, root morphology, leaf photosynthetic physiology, and root columbin levels in T. sagittata. These arbuscular mycorrhizal fungi (AMF) were able to colonize the roots, as evidenced by a root mycorrhizal colonization rate ranging from 17% to 48% and soil hyphal lengths ranging from 17.51 cm/g to 32.02 cm/g, with the Mix treatment being the greatest. AMF inoculations improved plant height (16–151%), leaf number (119–283%), shoot (37–211%), and root biomass (22–318%) to varying extents, with Ri and Mix treatments being the most prominent. AMF-treated plants presented relatively greater root total length, projected area, surface area, volume, and average diameter, especially those treated with Ri and Mix. AMF inoculations also significantly improved the leaf nitrogen balance index, transpiration rate, and stomatal conductance, while the photosynthesis rate and chlorophyll index varied by AMF species, along with a decrease in intercellular CO2 levels. Root columbin levels ranged from 0.524 mg/g to 5.389 mg/g, and AMF inoculation significantly increased root columbin levels by 228–928%, with Ri being the most significant. Root columbin levels were significantly positively correlated with soil hyphal length, root total length, root projected area, root surface area, root volume, and root average diameter, but not root AMF colonization rate. This study demonstrates for the first time that AMF, especially Ri, can be employed as a biostimulant to promote growth as well as root columbin levels in T. sagittata, where AMF-triggered improvement in root morphology is an important reason for promoting root columbin levels.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":"73 ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139175334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interaction of Culture Medium and Artificial Light Type on Pigmentation of Micro-Propagated Opuntia Plants 培养基和人工光照类型对微繁殖欧蓬植物色素沉着的影响
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-17 DOI: 10.3390/horticulturae9121348
Hector Silos Espino, R. Escalera García, D. Moncada González, L. L. Valera-Montero, S. Flores Benítez, M. Ortiz Morales, H. S. Guzmán Maldonado, Nivia Escalante-García, E. Olvera-Gonzalez
{"title":"Interaction of Culture Medium and Artificial Light Type on Pigmentation of Micro-Propagated Opuntia Plants","authors":"Hector Silos Espino, R. Escalera García, D. Moncada González, L. L. Valera-Montero, S. Flores Benítez, M. Ortiz Morales, H. S. Guzmán Maldonado, Nivia Escalante-García, E. Olvera-Gonzalez","doi":"10.3390/horticulturae9121348","DOIUrl":"https://doi.org/10.3390/horticulturae9121348","url":null,"abstract":"Opuntia is recognized economically as a significant crop for its nutritional, functional, and therapeutic properties and its potential in the pharmaceutical, cosmetic, and bioenergy industries. Opuntia is conventionally propagated by seed or vegetative propagation via rooted scions or grafting. However, multiplication procedures are insufficient for extensive spreading. One of the alternative techniques is in vitro. For this reason, the objective was to evaluate the growth of different components combined with light conditions to induce callus, embryogenesis, and the possible synthesis of a component in the genus Opuntia. The existence of genes involved in pigment synthesis in genotypes of different fruit colors was complementarily analyzed. In this study, we used different combinations of fructose (2 g/L) with prickly pear juice (2 mL/L), mannitol (4 g/L), silver nitrate (AgNO3: 1 mg/L), 2,4-dichlorophenoxyacetic acid (2,4-D: 2 mg/L), and indole-3-acetic acid (IAA: 1 g/L), with white, blue, and red LEDs (light-emitting diodes) and laser beams. These explants yielded sufficient contents of simple phenols, gallic acid equivalents (GAE: 2283.30 ± 6.29 mg/100 g), and coumaric (2155.0 ± 35.0 mg/100 g) and ferulic (2176 ± 27.9 mg/100 g) acids for the genotype Tapón aguanoso, and chlorogenic acid (CGA: 380.22 ± 22.05 mg/100 g) for the Copena V1 genotype. Upon analyzing the genotypes of different fruit pigmentations, we also observed the following: enzyme 4,5 estradiol dioxygenases can be present in all genotypes (regardless of pigmentation); enzyme 5,3 glucosyltransferases (GTs) could be induced in pigment synthesis in the genotypes of orange to purple fruits. Sequencing primer-amplified fragments for GT showed high similarity to uridine diphospho (UDP)-glucose from other species. This allows us to infer that it is possible to obtain products of high therapeutic value in the near future under controlled conditions.","PeriodicalId":13034,"journal":{"name":"Horticulturae","volume":"1 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138966498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Three-Dimensional Imaging in Agriculture: Challenges and Advancements in the Phenotyping of Japanese Quinces in Latvia 农业三维成像:拉脱维亚日本榅桲表型分析的挑战与进步
IF 3.1 3区 农林科学
Horticulturae Pub Date : 2023-12-17 DOI: 10.3390/horticulturae9121347
E. Kaufmane, Edgars Edelmers, K. Sudars, Ivars Namatēvs, A. Nikulins, S. Strautiņa, I. Kalniņa, Astile Peter
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