光周期和赤霉素对不抽薹大白菜茎叶和开花的影响

IF 3.1 3区 农林科学 Q1 HORTICULTURE
Shuping Liu, Junyang Lu, Jun Tian, Ping Cao, Shuhao Li, Haicui Ge, Mingxuan Han, Fenglin Zhong
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引用次数: 0

摘要

不抽头大白菜(小白菜)是一种重要的绿叶蔬菜,抽薹和开花是其繁殖的必要条件。然而,光周期对小白菜萌芽和开花的影响,尤其是对茎秆发育的影响,还需要进一步研究。在本研究中,我们进行了表型分析,并测量了甘蓝中的内源赤霉素水平。我们在 12 小时(光照)/12 小时(黑暗)、14 小时(光照)/10 小时(黑暗)、16 小时(光照)/8 小时(黑暗)和 18 小时(光照)/6 小时(黑暗)四种不同的光周期处理下进行了上述实验。结果表明,随着光照时间的增加,抽薹和开花的时间逐渐缩短。在 16 小时(光照)/8 小时(黑暗)的光周期处理下,茎的发育达到最佳状态,细胞学观察也得到了相同的结果。此外,不同光周期处理下内源赤霉素3(GA3)含量的变化与茎的发育一致,并在16小时(光照)/8小时(黑暗)时达到峰值。同时,qRT-PCR 分析表明,赤霉素合成酶关键基因 BcGA3ox2 和 BcGA20ox2 的相对表达量出现上调。当使用外源 GA3 及其合成抑制剂 paclobutrazol(PAC)处理时,外源赤霉素能显著促进小白菜的抽薹;反之,赤霉素抑制剂能抑制小白菜的抽薹、开花和茎伸长。因此,光周期可能通过调节内源 GA3 来调节甘蓝的勃起。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Photoperiod and Gibberellin on the Bolting and Flowering of Non-Heading Chinese Cabbage
Non-heading Chinese cabbage (cabbage) is an essential green leafy vegetable, and bolting and flowering are necessary for reproduction. However, further research is needed to study the effect of photoperiod on the bolting and flowering of cabbage, particularly on the development of the stem. In this study, we performed phenotypic analysis and measured endogenous gibberellin levels in the cabbage. We carried out these experiments under four different photoperiodic treatments, 12 h (light)/12 h (dark), 14 h (light)/10 h (dark), 16 h (light)/8 h (dark), and 18 h (light)/6 h (dark). The results showed that the time of bolting and flowering gradually decreased with increasing light duration. The development of stems was optimal under the 16 h (light)/8 h (dark) photoperiod treatment, and the same result was obtained via cytological observation. In addition, the changes in the endogenous gibberellin3 (GA3) content under different photoperiodic treatments were consistent with the development of stems and peaked at 16 h (light)/8 h (dark). At the same time, qRT-PCR analysis showed that the relative expression of the key gibberellin synthase genes, BcGA3ox2 and BcGA20ox2, exhibited upregulation. When treated with exogenous GA3 and its synthesis inhibitor, paclobutrazol (PAC), exogenous gibberellins significantly promoted bolting; conversely, gibberellin inhibitors suppressed the bolting, flowering, and stem elongation of cabbage. Therefore, the photoperiod may regulate cabbage bolting by regulating endogenous GA3.
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来源期刊
Horticulturae
Horticulturae HORTICULTURE-
CiteScore
3.50
自引率
19.40%
发文量
998
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