Hematology, Transfusion and Cell Therapy最新文献_第3页

Spontaneous epidural hematoma in a patient with sickle cell anemia - Case report 镰状细胞贫血患者自发性硬膜外血肿 - 病例报告

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2023.09.2369

Victoria Carneiro Lintz, Patricia Belintani Blum

引用次数: 0

Pseudogaucher cells in a patient with α-thalassemia minor and S-hemoglobin carrier 原发灶不明的癌症骨髓转移中的破骨细胞

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2023.11.010

Verónica Roldán Galiacho , Maite Moreno Gamiz , Juan Carlos García-Ruiz

引用次数: 0

Comparative evaluation of rapid plasma reagin and ELISA with Treponema pallidum hemagglutination assay for the detection of syphilis in blood donors: a single center experience 在检测献血者梅毒方面，快速血浆试剂和酶联免疫吸附试验与苍白螺旋体血凝试验的比较评估：一个单一中心的经验。

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2024.01.003

Brinda Kakkar, Joseph Philip, Rajeevinder Singh Mallhi

{"title":"Comparative evaluation of rapid plasma reagin and ELISA with Treponema pallidum hemagglutination assay for the detection of syphilis in blood donors: a single center experience","authors":"Brinda Kakkar, Joseph Philip, Rajeevinder Singh Mallhi","doi":"10.1016/j.htct.2024.01.003","DOIUrl":"10.1016/j.htct.2024.01.003","url":null,"abstract":"<div><h3>Introduction</h3><div>The prime responsibility of blood transfusion services in India is to provide safe blood. The donated blood is tested for human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), malaria and syphilis. In India, the screening of donated blood for syphilis is performed by rapid plasma reagin (RPR) or venereal disease research laboratory (VDRL), whereas the World Health Organization (WHO) recommends screening of syphilis in blood donors by enzyme-linked immunosorbent assay (ELISA). Therefore, the aim of this study was to evaluate the performance of RPR and ELISA with the <em>Treponema pallidum</em> hemagglutination assay (TPHA – the gold standard) for the detection of syphilis in blood donors.</div></div><div><h3>Methods</h3><div>In this cross-sectional study, 1524 consecutive whole blood donors were screened from April to October 2022. All blood samples collected during the study period were tested by RPR, ELISA and the TPHA and the results obtained were compared.</div></div><div><h3>Results</h3><div>The seroprevalence of syphilis in blood donors in this study was 0.06% by RPR and 0.72% by ELISA and TPHA. On considering ELISA and the TPHA as the gold standard, ELISA had comparable sensitivity (100%), a higher specificity (100% vs. 99.34%), a higher positive predictive value (PPV - 100% vs. 9.1%) and no biological false positive/false negative results (0 vs. 10 false negatives) when compared to RPR.</div></div><div><h3>Conclusion</h3><div>ELISA performed better as a screening assay than RPR in the detection of syphilis in blood donors, which is in agreement with the WHO recommendations for syphilis testing in blood donors with low prevalence.</div></div>","PeriodicalId":12958,"journal":{"name":"Hematology, Transfusion and Cell Therapy","volume":"46 ","pages":"Pages S43-S47"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140013800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outcomes of upfront plerixafor use for mobilization in germ cell tumor patients 生殖细胞肿瘤患者前期使用普乐沙福进行动员的效果。

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2023.12.008

Jaimie Cohen, Shreya Shah, Katrina Piedra, Sila Shalhoub, Cara Benjamin, Denise Pereira

{"title":"Outcomes of upfront plerixafor use for mobilization in germ cell tumor patients","authors":"Jaimie Cohen, Shreya Shah, Katrina Piedra, Sila Shalhoub, Cara Benjamin, Denise Pereira","doi":"10.1016/j.htct.2023.12.008","DOIUrl":"10.1016/j.htct.2023.12.008","url":null,"abstract":"<div><div>High-dose chemotherapy with autologous hematopoietic stem cell transplantation (auto-HSCT) improved 5-year overall survival rates in relapsed/refractory germ cell tumors (GCTs) from 10% to 52%. Nearly 30% of GCT patients are deemed poor mobilizers after receiving several lines of prior therapy. There is limited data available regarding upfront plerixafor use in GCT patients. We predicted upfront plerixafor use would increase the amount of stem cells collected preventing subsequent mobilizations and improve time to curative therapy. A retrospective, single center, chart review of adult GCT patients who received plerixafor upfront for mobilization at a single center between January 1, 2013 and August 31, 2021 was performed.</div><div>The primary objective was to evaluate the rate of successful peripheral blood CD34<sup>+</sup> cell collections. Secondary objectives consisted of describing the impact of plerixafor use on mobilization and assessing auto-HSCT related outcomes. Sixteen patients received plerixafor upfront after an average of three prior lines of therapy (range: 2-5 lines). Successful collection (≥4 × 10<sup>6</sup> CD34<sup>+</sup> cells/Kg collected within four days) was achieved in 15 (94%) patients in a median of one apheresis day (interquartile range: 1-2 days). All patients proceeded to an initial auto-HSCT and 12 patients (75%) completed both transplants as planned. Survival at 12 months was 50%.</div><div>The significantly higher amount of CD34<sup>+</sup> cells collected over less apheresis days demonstrated the clinical utility of upfront plerixafor and its potential to facilitate more efficient stem cell mobilization. There is a need for larger randomized studies with upfront plerixafor use in this unique patient population.</div></div>","PeriodicalId":12958,"journal":{"name":"Hematology, Transfusion and Cell Therapy","volume":"46 ","pages":"Pages S37-S42"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140133607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmodium ovale gametocytes in peripheral blood 外周血中的卵形疟原虫配子细胞

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2023.11.017

Angela Lozano-Ochoa , Verónica Roldán Galiacho , Leyre Cristina Jiménez Ros , Raquel Pérez Garay , Juan Carlos García-Ruiz

引用次数: 0

An instrument of screening criteria to assess individualized high-risk sexual behavior to be applied in the Brazilian population: translation and cross-cultural adaptation 用于评估巴西人口个性化高风险性行为的筛查标准工具：翻译和跨文化调整。

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2024.06.009

Miriane Lucindo Zucoloto , Guilherme Galdino , Edson Zangiacomi Martinez

{"title":"An instrument of screening criteria to assess individualized high-risk sexual behavior to be applied in the Brazilian population: translation and cross-cultural adaptation","authors":"Miriane Lucindo Zucoloto , Guilherme Galdino , Edson Zangiacomi Martinez","doi":"10.1016/j.htct.2024.06.009","DOIUrl":"10.1016/j.htct.2024.06.009","url":null,"abstract":"<div><h3>Background</h3><div>Alternative approaches have been proposed to ensure a safe and equitable screening process for blood donation that treats all people equally, regardless of gender identity or sexual orientation. The terms ‘neutral approach’ and ‘individualized risk assessment’ have been used to describe this goal. To facilitate research and implementation of these concepts in blood donation contexts and health services in Brazil, we propose a Portuguese version of the ‘for the assessment of individualized risk screening criteria’ (FAIR) screening criteria.</div></div><div><h3>Methods</h3><div>The FAIR screening criteria are 12 questions that assess sex, sexuality, ethnicity, and the extent to which participants engaged in each targeted sexual behavior. The aim of FAIR is to reduce error while increasing reliable and accurate reporting of sexual behaviors associated with both objective and subjective estimates of infection risk. The FAIR screening criteria were translated and cross-culturally adapted using a systematic approach with standardized procedures appropriate for adapting instruments that track behaviors.</div></div><div><h3>Results</h3><div>A version that is appropriate for use with the Brazilian population was produced employing the following steps: expert translations, harmonization, consensus version, expert back-translation, revision, panel of experts, cognitive interviewing, and finalization.</div></div><div><h3>Conclusion</h3><div>The Portuguese version of FAIR was proposed, and because of its straightforward, simple language and focus on specific and frequent behaviors in some populations, it has the potential to be used in a variety of contexts involving the screening of high-risk sexual behavior in Brazil.</div></div>","PeriodicalId":12958,"journal":{"name":"Hematology, Transfusion and Cell Therapy","volume":"46 ","pages":"Pages S122-S127"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance and safety of therapeutic erythrocytapheresis in polycythemia and hemochromatosis treatment: single centre experience 在多血症和血色素沉着症治疗中使用治疗性红细胞透析的效果和安全性：单中心经验。

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2024.05.011

Iva Lucija Burnać , Ines Bojanić , Sanja Mazić , Marija Lukić , Branka Golubić Ćepulić

{"title":"Performance and safety of therapeutic erythrocytapheresis in polycythemia and hemochromatosis treatment: single centre experience","authors":"Iva Lucija Burnać , Ines Bojanić , Sanja Mazić , Marija Lukić , Branka Golubić Ćepulić","doi":"10.1016/j.htct.2024.05.011","DOIUrl":"10.1016/j.htct.2024.05.011","url":null,"abstract":"<div><h3>Introduction</h3><div>Therapeutic erythrocytapheresis has some advantages over therapeutic phlebotomy, the standard treatment for cytoreduction in polycythemia and hemochromatosis. Erythrocytapheresis can be performed on different cell separators, each with its own characteristics. We present our experience of therapeutic erythrocytapheresis in the treatment of polycythemia and hemochromatosis with an analysis of the performance of cytoreduction, and a comparison between the characteristics of intermittent- and continuous-flow cell separators.</div></div><div><h3>Material and methods</h3><div>During a 20-year period, 1731 procedures were performed in 125 patients, 1634 (94.4%) with a Haemonetics MCS+ separator and 97 (5.6%) with a Spectra Optia system device. The performance of cytoreduction using the Haemonetics MCS+ separator was analysed in 442 procedures performed in 56 patients and the performance of the two apheresis devices was compared.</div></div><div><h3>Results</h3><div>Haemoglobin (Hb) and haematocrit (Hct) values were significantly reduced after erythrocytapheresis with the Haemonetics MCS+ device (Hb: 18.69%; Hct: 18.73%; p-values both <0.001). The reductions of Hb and Hct were significantly higher in the Haemonetics MCS+ procedure (p-value <0.001), but the Spectra Optia procedure depleted a significantly higher RBC volume (495 mL versus 442 mL) in a shorter time (18 min versus 36 min).</div></div><div><h3>Conclusion</h3><div>Both the Haemonetics MCS+ and Spectra Optia systems proved to be highly efficient and safe in RBC cytoreduction with short procedure times. Erythrocytapheresis reduces the frequency of necessary procedures thereby justifying its therapeutic use especially in eligible patients of working age.</div></div>","PeriodicalId":12958,"journal":{"name":"Hematology, Transfusion and Cell Therapy","volume":"46 ","pages":"Pages S103-S108"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142304645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improvement of blood transfusion safety using the chemiluminescence technique for viral marker screening of blood donors in sub Saharan Africa 利用化学发光技术对撒哈拉以南非洲的献血者进行病毒标记筛查，提高输血安全。

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2024.04.120

Macoura GADJI , Aissata BA , Youssou Bamar GUEYE , Alioune Badara SENGHOR , Tandakha Ndiaye DIEYE , Saliou DIOP

{"title":"Improvement of blood transfusion safety using the chemiluminescence technique for viral marker screening of blood donors in sub Saharan Africa","authors":"Macoura GADJI , Aissata BA , Youssou Bamar GUEYE , Alioune Badara SENGHOR , Tandakha Ndiaye DIEYE , Saliou DIOP","doi":"10.1016/j.htct.2024.04.120","DOIUrl":"10.1016/j.htct.2024.04.120","url":null,"abstract":"<div><h3>Introduction</h3><div>Sub-Saharan Africa struggles continuously with insufficient resources and inadequate infrastructure that hinder the establishment of a safer blood supply despite improvements in transfusion safety over recent decades. This study aimed to evaluate the impact of the chemiluminescence technique in combination with immunoenzymatic and immunochromatographic tests for viral marker screening of hepatitis B (HBV), hepatitis C (HCV) and human immunodeficiency virus (HIV) in donated blood in a country of sub-Saharan Africa.</div></div><div><h3>Method</h3><div>This study was conducted in a population of 113,406 blood donors at the National Centre of Blood Transfusion in Senegal. The data were obtained from the ‘INLOG’ software and donor registers. Statistical analyses used Excel 2010 and Epi Info v6. Screening for HBsAg viral markers, anti-HCV Ab, HIV p24 Ag, anti-HIV1 and anti-HIV2 antibodies were first carried out using the chemiluminescence technique. Blood donations screened positive for HBV or HCV were retested in a second chemiluminescence equipment. HIV-positive donations and their controls were subjected to solid phase immunochromatographic and indirect enzyme immunoassay techniques.</div></div><div><h3>Results</h3><div>The prevalence among donors of HBV was 8.39 %, 0.56 % for HCV and 0.18 % for HIV. Of the donors tested positive for HIV in screenings and in doubled-controls, only 61.54 % were confirmed by the alternative tests; 34.02 % were negative and 4.44 % discordant between the three techniques.</div></div><div><h3>Conclusion</h3><div>This study shows the importance of introducing the chemiluminescence technique in association with serological screening of transfusion-transmitted viruses to improve blood supply safety in low-income countries.</div></div>","PeriodicalId":12958,"journal":{"name":"Hematology, Transfusion and Cell Therapy","volume":"46 ","pages":"Pages S72-S79"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142335171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional characterization of compound heterozygosity Hb S/Hb Deer Lodge in Brazil 巴西 Hb S/Hb Deer Lodge 复合杂合子的功能特征

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2023.09.2370

Marcondes J. Sobreira , Thais H. Batista , Rodrigo M. Santana , Gabriela S. Arcanjo , Susan E. Jorge , Magnun N. Santos , Dulcinéia M. Albuquerque , Betânia L. Hatzlhofer , Ana C. dos Anjos , Fernando F. Costa , Aderson S. Araujo , Maria F. Sonati , Antonio R. Lucena-Araujo , Marcos A. Bezerra

{"title":"Functional characterization of compound heterozygosity Hb S/Hb Deer Lodge in Brazil","authors":"Marcondes J. Sobreira , Thais H. Batista , Rodrigo M. Santana , Gabriela S. Arcanjo , Susan E. Jorge , Magnun N. Santos , Dulcinéia M. Albuquerque , Betânia L. Hatzlhofer , Ana C. dos Anjos , Fernando F. Costa , Aderson S. Araujo , Maria F. Sonati , Antonio R. Lucena-Araujo , Marcos A. Bezerra","doi":"10.1016/j.htct.2023.09.2370","DOIUrl":"10.1016/j.htct.2023.09.2370","url":null,"abstract":"<div><h3>Introduction</h3><div>The Hb Deer Lodge (β2 His>Arg; HBB:c.8A>G) is a structural hemoglobin variant described in some populations around the world, characterized by increased oxygen affinity, but does not confer clinical symptoms to its carriers. The coinheritance of the Hb Deer Lodge with the most common hemoglobin variant, Hb S, has been reported only once; however, functional data were not described. Here we show a case of the Hb S and Hb Deer Lodge carrier in heterozygosity.</div></div><div><h3>Methods</h3><div>The Hb S and Hb Deer Lodge association was identified by High-Performance Liquid Chromatography (HPLC), reverse phase HPLC and the β globin gene sequencing. The functional characterization of this interaction was obtained using the O<sub>2</sub> dissociation curve, determination of the cooperativity between the globin chains and the Bohr effect in the presence and absence of organic phosphates.</div></div><div><h3>Results</h3><div>When the Hb S and Hb Deer Lodge were associated, there was a decrease in cooperativity, no significant changes in oxygen affinity and no significant Bohr effect changes.</div></div><div><h3>Conclusion</h3><div>Despite these genetic variations, the carrier showed no hematological alterations and no clinical symptoms, possibly due to the high oxygen affinity of the Hb Deer Lodge, which interferes with the Hb S polymerization.</div></div>","PeriodicalId":12958,"journal":{"name":"Hematology, Transfusion and Cell Therapy","volume":"46 ","pages":"Pages S158-S162"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138621524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of hydroxyurea on tubular phosphate handling in sickle cell nephropathy 羟基脲对镰状细胞肾病患者肾小管磷酸盐处理的影响

IF 1.8

Hematology, Transfusion and Cell Therapy Pub Date : 2024-11-01 DOI: 10.1016/j.htct.2023.11.015

Gabriela Araujo de Abreu, Duaran Lopes de Sousa, Suzzy Maria Carvalho Dantas, Alice Maria Costa Martins, Tiago Lima Sampaio, Romélia Pinheiro Gonçalves Lemes

{"title":"Influence of hydroxyurea on tubular phosphate handling in sickle cell nephropathy","authors":"Gabriela Araujo de Abreu, Duaran Lopes de Sousa, Suzzy Maria Carvalho Dantas, Alice Maria Costa Martins, Tiago Lima Sampaio, Romélia Pinheiro Gonçalves Lemes","doi":"10.1016/j.htct.2023.11.015","DOIUrl":"10.1016/j.htct.2023.11.015","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to evaluate the markers of tubular phosphate handling in adults with sickle cell anemia (SCA) and the influence of hydroxyurea (HU), the degree of anemia and Hb F concentration on these markers.</div></div><div><h3>Methods</h3><div>Eighty-eight steady state SCA patients in outpatient follow-up in Fortaleza, Ceara, Brazil and 31 healthy individuals were included in this study. Vitamin D (25OHD) was measured by enzyme-bound fluorescence assay, intact parathyroid hormone (iPTH) by electrochemiluminescence, and serum and urinary phosphate and creatinine by colorimetric methods. Details of Hb F and HU use were obtained from clinical records. Tubular reabsorption of phosphate (TRP) and maximum tubular reabsorption of phosphate (MTRP) were calculated. SCA patients were stratified according to the use of HU, degree of anemia and percentage of Hb F. The significance level was set for p-values <0.05.</div></div><div><h3>Results</h3><div>Compared to controls the 25OHD level (25 ± 11 vs. 30 ± 9 pg/mL) was lower in SCA, while serum phosphate and MTRP were higher (3.86 ± 0.94 vs. 3.46 ± 0.72 and 3.6 ± 1.21 vs. 3.21 ± 0.53, respectively). There was no significant difference in iPTH, TRP and phosphaturia. Serum phosphate showed correlation with TRP (<em>r</em> = 0.32; p-value = 0.008) and MTRP (<em>r</em> = 0.9; p-value <0.001) in SCA. Patients taking HU, especially those with Hb <em>F</em> >10 % presented reduced serum phosphate levels, and TRP and MTRP rates. Those with mild anemia presented reduced serum phosphate levels and MTRP rates.</div></div><div><h3>Conclusion</h3><div>Serum phosphate levels and renal phosphate reabsorption rate were increased in SCA. HU use, high Hb F concentration and total Hb were associated with better control of tubular phosphate handling markers.</div></div>","PeriodicalId":12958,"journal":{"name":"Hematology, Transfusion and Cell Therapy","volume":"46 ","pages":"Pages S163-S169"},"PeriodicalIF":1.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139878031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0