Hacettepe Journal of Biology and Chemistry最新文献

{"title":"Assessing of photoluminescence and structural properties of Dy+3 doped cadmium tantalate phosphor","authors":"Lütfiye Feray Güleryüz","doi":"10.15671/hjbc.1056363","DOIUrl":"https://doi.org/10.15671/hjbc.1056363","url":null,"abstract":"According to the charge balance, trivalent dysprosium doped Cdx-1Ta2O6:xDy3+ (x=0.5, 1, 2, 3, 5) phosphor series was fabricated by solid state reaction route at 1100 C for 8 h. The synthesized phosphors were investigated by XRD (X-ray diffraction), SEM (scanning electron microscopy), and PL (photoluminescence) analyses. XRD and SEM analyzes revealed the orthorhombic colombite crystal structure and the presence of oval-like and shapeless morphology with submicron and several micron grain sizes, respectively. With the excitation of 387.8 nm, the phosphors exhibited blue and yellow emissions at 482.4 nm and 578.5 nm depending on the 4F9/2→6H15/2 and 4F9/2→6H13/2 transitions of Dy3+, respectively. The concentration quenching did not occur because the improvement in the charge balance reduced the structural defects and decreased the conversion of excitation energy to non-radiative transitions. The emission intensity of cadmium tantalate phosphor increased with increasing Dy3+ ion concentration up to x=5 value, and did not occurred the concentration quenching which reduces emission intensity. The Commission Internationale d’Eclairage (CIE) coordinates of Cdx-1Ta2O6:xDy3+ (x=0.5, 1, 2, 3, 5) phosphors were found near the white region in the chromaticity diagram","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81274113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enjeksiyon Kalıplama ve Erimiş Filament Ekstrüzyonu (EFE) yöntemleri ile üretilen ahşap elyaf takviyeli PLA matris biyo-kompozitlerin karşılaştırılması","authors":"Alperen Doğru, Seçil Yilancioğlu, Görkem Ülkü, Buket ŞENTÜRK TURAN, M. Ö. Seydibeyoğlu","doi":"10.15671/hjbc.1053764","DOIUrl":"https://doi.org/10.15671/hjbc.1053764","url":null,"abstract":"Plastic materials have a variety of chemical and mechanical properties that will be useful for a wide variety of applications. However, plastic waste creates pollution and poses a great threat due to the problem of non-degradability affecting ecological environments. Thermoset polymers, which are used extensively in the polymer industry today, have recycling problems. This situation creates serious environmental problems. The most important feature of thermoplastic materials is that they can be recycled. The use of thermoplastic polymers creates significant advantages in recycling and environmental issues. The rapid evolution in additive manufacturing provides a new path to the circular economy using recycling. Thermoplastic or thermoset materials can be processed in additive manufacturing. \u0000Additive Manufacturing Methods (AM) are used today in different industries to produce prototypes and even final products. The polymer material is used in 51% of the parts produced with AM. The Fused Filament Fabrication (FFF) method is the most popular method preferred for shaping polymers with AM. The FFF method is a method of extruding a spool of thermoplastic filament through a heated nozzle and melting the material. Also, FFF is known to have low cost and high printing speeds compared to other AM techniques. PLA material, which is a completely bio-based thermoplastic polymer with many desirable properties, including easy processing ability, strength, hardness, and biodegradability, is widely used in material processing by the FFF method. \u0000In this study, the PLA matrix was reinforced with natural fibers to increase the mechanical properties and contribute to recycling. Bio-composite compounds with 15% wood fiber reinforced PLA matrix by weight were prepared. Specimens’ productions were carried out using bio-composite materials, the FFF method, and injection molding methods. Thermal analyzes of the prepared compounds, filaments, and produced specimens were carried out. A decrease in the Tg value of the compound reinforced with natural fiber was observed, while an increase in the Tm value was observed. The Tg value of the specimens produced by the FFF method increased compared to the injection specimens. In addition, the mechanical properties of the specimens produced by FFF, and the injection molding method were compared. It was determined that the stress at break values of the specimens produced by injection were 2 times higher than the specimens produced by FFF. The impact strength of the specimens produced with injection molding is 51.75% higher than the specimens produced with FFF. The bio-composite materials produced in the study were examined under scanning electron microscopy (SEM). Surface interactions and homogeneous fiber distribution between matrix and fiber were investigated.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78907877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesis of Silver Nanoparticles Using Walnut Shell Powder and Cynara sp. and their Antibacterial Activities","authors":"I. Erdem, Şerife Çakir","doi":"10.15671/hjbc.984727","DOIUrl":"https://doi.org/10.15671/hjbc.984727","url":null,"abstract":"The silver (Ag) is a well-known material with interesting properties (i.e. catalytic activity, antimicrobial, etc.). The nano-sized particles of silver propose enhanced properties due to having relatively higher surface areas. The green synthesis is a promising way of material preparation/production being relatively more environmentally friendly by utilization of less harmful materials. In this work, the plant extracts (Cynara & Walnut shell powder) were used as reaction media for the synthesis of silver nanoparticles (Ag NPs). The nanoparticles produced via two plant extracts were ~46 nm and ~109 nm in size, respectively. The antibacterial activities of the produced silver nanoparticles (against E. coli and S. aureus species) were determined and minimum effective concentrations (MIC) for antibacterial activity were investigated.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79075249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bir Etkili Tek Ortamda Üç Bileşenli Yeni Kinazolin-4-Karboksilik Asit ve Onun Ester ve Amit Türevlerinin Sentezi","authors":"Derviş Gök","doi":"10.15671/hjbc.1004758","DOIUrl":"https://doi.org/10.15671/hjbc.1004758","url":null,"abstract":"İlaç adayları olabilecek bir dizi yeni kinazolin türevleri sentezlendi ve yapıları IR, 1H NMR, 13C NMR ve Q-TOF LC/MS spektrometresi ile karakterize edildi. Önce amonyum asetat ve 4-klorobenzaldehit ile isatinin (indol-2,3-dion) hidrolizinden elde edilen (2-amino-fenil)-okso-asetik asit sodyum tuzunun aynı ortamda üç bileşenli kondenzasyon reaksiyonundan 2-(4-kloro-fenil)-kinazolin-4-karboksilik asit (2) sentezlendi. Karboksilik asit bileşiği 2, ester, asit klorür ve amit türevlerinin sentezine izin verdi. Yeni kinazolin ester türevleri (3-6, 8), 2 bileşiği ve çeşitli alkollerin reaksiyonlarından sentezlendi. Daha sonra kinazolin amit türevleri (9-13), çeşitli alifatik ve aromatik aminlerin 2 bileşiği ve SOCl2’ün reaksiyonundan oluşan 2-(4-kloro-fenil)-kinazolin-4-karbonil klorürün (7) reaksiyonundan elde edildi.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85643391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"siRNA targeting ABCB1 potentiates the efficacy of chemotherapy in human triple-negative breast cancer cells","authors":"Goknur Kara","doi":"10.15671/hjbc.975466","DOIUrl":"https://doi.org/10.15671/hjbc.975466","url":null,"abstract":"Diminishing the efficacy of chemotherapy because of multidrug resistance (MDR) is a major clinical problem for triple-negative breast cancer (TNBC). MDR often occurs by overexpression of ATP-binding cassette B1 (ABCB1) protein that effuses various anticancer drugs from cancer cells. One of the newly developed techniques to addressing MDR is to knockdown ABCB1 by RNA interference (RNAi). RNAi is a gene-silencing process in that small interfering RNA (siRNA) blocks the expression of desired genes with high efficiency/specificity. The aim of this work is to examine the impact of ABCB1 inhibition via specific siRNAs on the efficacy of paclitaxel or etoposide in TNBC cells. The toxicity of increasing paclitaxel and etoposide concentrations on MDA-MB-231 cells was assessed using the MTT test. Cells were then co-treated with paclitaxel or etoposide in combination with ABCB1-siRNA, followed by cytotoxicity, colony formation, and migration assays. The administration of ABCB1-siRNA with paclitaxel or etoposide exhibited a synergistic effect and siRNA-drug treatments markedly reduced viability, clonogenicity, and migration of TNBC cells compared to siRNA or drug alone. Overall, these results indicate that TNBC cells become vulnerable even to sub-toxic doses of paclitaxel and etoposide after ABCB1-siRNA transfection, representing a promising aproach to enhance the influence of chemotherapy in TNBC.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88948793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How mesenchymal stem cell conditioned media affect the HeLa cells on Wnt/beta-catenin signaling, Notch-1 signaling, and apoptosis?","authors":"Hanife Guler Donmez, Handan SEVİM AKAN","doi":"10.15671/hjbc.1001427","DOIUrl":"https://doi.org/10.15671/hjbc.1001427","url":null,"abstract":"This study aims to investigate the influence of mesenchymal stem cells (MSCs) cell-conditioned media (MSCs-CM) on the Wnt/beta-catenin and Notch-1 signaling as well as the apoptosis in cervical cancer cells. Conditioned media of characterized MSCs were freshly collected and filtered before use. HeLa cells cultured standard conditions and treated with MSCs-CM 24, 48, 72 hours. Untreated cells serve as a control. Cell viability measured with MTT assay for all incubation periods. Immunocytochemical staining of beta-catenin, Notch-1 and cleaved caspase 3 were performed for each time-point. MTT cell viability, AO/PI, and immunocytochemical staining of cleaved caspase 3 results showed that through all incubation periods, there was no statistically significant difference between the MSCs-CM treated HeLa cells and the controls (p>0.05). Beta-catenin immunoreactivity was upregulated following treatment from 24 hours to 48 and 72 hours (p","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80588669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, Characterization, Investigation of AChE Activities and Molecular Docking Studies of New Schiff Bases Based on Substitute Salicyl Aldehyde","authors":"İ. Şahin","doi":"10.15671/hjbc.1020606","DOIUrl":"https://doi.org/10.15671/hjbc.1020606","url":null,"abstract":"","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88787947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Composite Barrier in Radioactive Waste Repositories","authors":"Sema Erentürk, Rüveyda Kübra İleri Durmuş","doi":"10.15671/hjbc.1032456","DOIUrl":"https://doi.org/10.15671/hjbc.1032456","url":null,"abstract":"Bu çalışmada, sulu bir çözeltiden 126Sn ve 79Se radyonüklidlerin uzaklaştırılması için PAN/Zeolit bazlı kompozit bariyer malzemesinin performansı değerlendirildi. Kompozit bariyer malzemesinin performans değerlendirmesi 126Sn ve 79Se radyonüklidleri için SWOT analizi kullanılarak ayrı ayrı yapılmıştır. Ayrıca, radyoaktif atık depolama alanlarından bu radyonükltlerin yanlışlıkla salınması durumunda, insan dışı canlılara yönelik riskler değerlendirilmiştir. Bu riskler ERICA assessment tool kullarak tatlı su ve karasal biotaların alacağı toplam dozlar hesaplanarak değerlendirilmiştir. Tatlı sudaki kabuklular, fitoplankton ve vasküler bitkiler gibi referans biyotaların maruz kaldığı toplam doz aralığı, kompozit bariyer malzemesi kullanılarak 126Sn izotopu için 4.02E6-3.87E9 µG h-1'e ve 79Se izotopu için 3.35E07-1.92E12 µG h-1'e düşürülmüştür. Annelidler, çimenler, likenler ve briyofitler, memeliler ve ağaçlar gibi karasal referans biyotasında, maruz kalınan toplam doz aralığı 126Sn için 7.41E04-4.51E06 µG h-1ya ve 79Se için 2.15E06-5.53E09 µG h-1'e düşürülmüştür.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81987996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation Cobalt Ferrite–Silver Nanocomposite Via Green Chemistry Approach","authors":"Yeliz Akpınar, Begüm Avci","doi":"10.15671/hjbc.1032223","DOIUrl":"https://doi.org/10.15671/hjbc.1032223","url":null,"abstract":"Nanocomposites are formed by joining two or more materials, at least one of which must have a size in the nanometer range (1 nm -100 nm). In this study, cobalt ferrite-silver nanocomposite was prepared with a green synthesis approach. The aim was to combine the magnetic properties of cobalt-ferrite nanoparticles with both the antimicrobial and conductivity properties of silver nanoparticles. Thus, the prepared material can be used for biological, optical, electrical, and magnetic applications. Cobalt ferrite nanoparticles were prepared using the co-precipitation method. Silver nanostructures were incorporated into cobalt ferrite nanoparticles using waste grass extract. Scanning Electron Microscope (SEM), Infrared Spectrometer, UV-VIS Spectrometer, X-RAY Diffraction Spectrometer, Dynamic Light Scattering Technique were used for characterization studies.","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"2011 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74053490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinspired Material-Integrated Sensors for Improving Nanoplasmonic Characteristics","authors":"F. Inci","doi":"10.15671/hjbc.1035918","DOIUrl":"https://doi.org/10.15671/hjbc.1035918","url":null,"abstract":"","PeriodicalId":12939,"journal":{"name":"Hacettepe Journal of Biology and Chemistry","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83939404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}