Haemostasis最新文献_第7页

Determination of the prevalence of fibrinogen Banks peninsula mutation (gamma280Tyr-->Cys) by PCR. 用PCR方法测定纤维蛋白原Banks半岛突变(gamma280Tyr- >Cys)的患病率。

Haemostasis Pub Date : 1999-01-01 DOI: 10.1159/000022506

K Zimmermann, P L Turecek, H P Schwarz

引用次数: 0

Usefulness of screening for congenital or acquired hemostatic abnormalities in women with previous complicated pregnancies. 筛查先天性或获得性止血异常对既往复杂妊娠妇女的有用性。

Haemostasis Pub Date : 1999-01-01 DOI: 10.1159/000022502

G Mello, E Parretti, E Martini, F Mecacci, P La Torre, R Cioni, R Lucchetti, S Fedi, A M Gori, G Pepe, D Prisco, R Abbate

引用次数: 53

Protein-bound heparin/heparan sulfates in human adult and umbilical cord plasma. 成人和脐带血浆中的蛋白结合肝素/硫酸肝素。

Haemostasis Pub Date : 1999-01-01 DOI: 10.1159/000022508

H Xiao, S J Miller, N U Bang, W P Faulk

{"title":"Protein-bound heparin/heparan sulfates in human adult and umbilical cord plasma.","authors":"H Xiao, S J Miller, N U Bang, W P Faulk","doi":"10.1159/000022508","DOIUrl":"https://doi.org/10.1159/000022508","url":null,"abstract":"We used thrombin times and a competitive radiometric assay to identify, quantitate and characterize endogenous heparin-like molecules in umbilical cord (n = 58) and normal adult (n = 25) plasma. Thrombin times for cord plasma (29.6+/-3.6 s) were significantly longer (p< or = 0.0005) than those for adult plasma (18. 9+/-2.3 s), suggesting increased endogenous heparins. A radiometric assay based on the displacement of (125)I-heparin from protamine-Sepharose revealed that protease-digested plasma contained heparin/heparan sulfate, and plasma that was not digested with protease appeared not to contain heparin/heparan sulfate. More heparin/heparan sulfate was identified in cord than in adult plasma (p< or =0.05), but heparinase digestion produced significantly (p< or =0.001) reduced concentrations of heparin/heparan sulfate in only 39% of the samples. The lack of heparinase sensitivity in 61% of the protease-digested samples apparently was due to low molecular weight (LMW) heparins, for control heparin fragments of 5 kD that did not extend thrombin times were also less affected by heparinase, but the same LMW heparins were detected by radiometric assay. Despite normal thrombin times in all samples, the amounts of endogenous heparin/heparan sulfate identified in protease-digested samples by radiometric assay were of sufficient concentrations to produce inordinately prolonged thrombin times when compared with the same concentrations of unfractionated heparin. Collectively, these findings suggest the presence of a plasma reservoir of endogenous heparin/heparan sulfates in normal cord and adult plasma. These endogenous heparin/heparan sulfates are bound to plasma proteins, and an as yet undetermined proportion of these bound heparin/heparans are most likely LMW molecules.","PeriodicalId":12910,"journal":{"name":"Haemostasis","volume":"29 4","pages":"237-46"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000022508","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21555070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effect of ticlopidine on platelet-derived microparticles in patients with connective tissue diseases. 噻氯匹定对结缔组织病患者血小板源性微粒的影响。

Haemostasis Pub Date : 1999-01-01 DOI: 10.1159/000022510

H Kagawa, S Nomura, M Nagahama, Y Ozaki, S Fukuhara

引用次数: 15

The FVII activating protease cleaves single-chain plasminogen activators. FVII激活蛋白酶裂解单链纤溶酶原激活物。

Haemostasis Pub Date : 1999-01-01 DOI: 10.1159/000022515

J Römisch, S Vermöhlen, A Feussner, H Stöhr

{"title":"The FVII activating protease cleaves single-chain plasminogen activators.","authors":"J Römisch, S Vermöhlen, A Feussner, H Stöhr","doi":"10.1159/000022515","DOIUrl":"https://doi.org/10.1159/000022515","url":null,"abstract":"A serine protease isolated from plasma sharing structural characteristics with a hepatocyte growth factor activator-like protease has been demonstrated recently to activate FVII. Accordingly, it was named ‘FVII activator’. Until now an impact of this protease on the fibrinolytic system has not been reported. We islolated the protease from cryo-poor plasma by subsequent ion exchange chromatography and adsorption to immobilized heparin and/or aprotinin. Incubation of single-chain plasminogen activators (sc-PAs) with the FVII activator revealed significant activation of urokinase sc-PA (scu-PA) and tissue sc-PA (sct-PA) in vitro. It was enhanced in the presence of calcium and heparin. Compared to kallikrein, a more efficient activation of scu-PA was observed, whereas sct-PA appeared to be a poorer substrate for the FVI activator. At low protease concentrations and in the presence of heparin the scu-PA activation was comparable to plasmin. Employing recalcified whole blood thrombelastography, the lysis of initially formed fibrin was observed after addition of a combination of scu-PA and the FVII activator, whereas the scu-PA alone had a negligible effect at the concentration used. The study results as presented demonstrate that the FVII activator is a potent activator of sc-PAs in vitro. Whether it plays a physiological role in fibrinolysis deserves further investigation. Its comparatively high affinity to heparin assumes a function in cell surface or matrix events.","PeriodicalId":12910,"journal":{"name":"Haemostasis","volume":"29 5","pages":"292-9"},"PeriodicalIF":0.0,"publicationDate":"1999-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000022515","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21605146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 123

Relative and absolute changes of activated platelets, microparticles and platelet aggregates after activation in vitro. 体外活化后活化血小板、微粒和血小板聚集体的相对和绝对变化。

Haemostasis Pub Date : 1998-11-01 DOI: 10.1159/000022444

A C Matzdorff, D Berchner, G Kühnel, B Kemkes-Matthes, H Pralle, R Voss

{"title":"Relative and absolute changes of activated platelets, microparticles and platelet aggregates after activation in vitro.","authors":"A C Matzdorff, D Berchner, G Kühnel, B Kemkes-Matthes, H Pralle, R Voss","doi":"10.1159/000022444","DOIUrl":"https://doi.org/10.1159/000022444","url":null,"abstract":"Standard flow cytometers provide relative numbers of activated platelets, microparticles, and platelet aggregates. With fluorescent beads it is now possible to determine absolute numbers. Whole blood and platelet-rich plasma were incubated with agonists (ADP, collagen, thrombin). CD62p expression, microparticle and platelet aggregate formation were measured. Flow-Count Fluorospheres((R)) were added to calculate absolute concentrations. After activation there was an increase in the percentage of CD62p-positive platelets. However, the total number of platelets decreased and therefore the absolute number of CD62p-positive platelets did not increase but decreased. The number of CD62p-positive platelets decreased not as much as the number of CD62p-negative platelets, which explains why the relative percentage of CD62p-positive platelets increased. A similar increase in percent and decrease in absolute counts was found for microparticles. Platelet aggregates increased both in relative and absolute numbers. These results suggest that the detection of activated platelets by flow cytometry has to be complemented by the determination of the absolute concentrations to avoid misinterpretation.","PeriodicalId":12910,"journal":{"name":"Haemostasis","volume":"28 6","pages":"277-88"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000022444","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21325780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Treatment of Venous Thromboembolism. 静脉血栓栓塞的治疗。

Haemostasis Pub Date : 1998-11-01

引用次数: 0

Elevated thrombotic activity after myocardial infarction: A 2-year follow-up study. 心肌梗死后血栓活性升高:一项2年随访研究。

Haemostasis Pub Date : 1998-11-01 DOI: 10.1159/000022446

V Martínez-Sales, V Vila, E Réganon, M A Goberna, F Ferrando, M A Palencia, J Aznar

{"title":"Elevated thrombotic activity after myocardial infarction: A 2-year follow-up study.","authors":"V Martínez-Sales, V Vila, E Réganon, M A Goberna, F Ferrando, M A Palencia, J Aznar","doi":"10.1159/000022446","DOIUrl":"https://doi.org/10.1159/000022446","url":null,"abstract":"This study examines the evolution of the thrombotic activity in patients with myocardial infarction (MI) treated with aspirin (200 mg/day) for 2 years after MI. Plasma samples of 10 patients were collected at 7, 30, 60, 90, 120, 150, 180, 360 and 720 days. In all the samples we measured fibrinogen (Fg), high molecular weight Fg (HMW-Fg), fibrinopeptide A (FPA), prothrombin fragment 1+2 (F1+2), beta-thromboglobulin (beta-TG), von Willebrand factor (vWF), tissue factor (TF) and TF pathway inhibitor (TFPI). The plasma Fg, HMW-Fg, FPA, F1+2, beta-TG and vWF levels were significantly elevated in the patients at the beginning of the study as compared to the normal group. The 95% confidence intervals were Fg 277-333 mg/dl, HMW-Fg 200-244 mg/dl, FPA 5.3-16.5 ng/ml, F1+2 1.4-1.8 nmol/l, beta-TG 110-118 IU/ml and vWF 139-195%. At thirty days Fg and HMW-Fg returned to normal levels, whereas the increase in FPA and F1+2 levels persisted throughout the study. At 120 and 150 days, respectively, beta-TG and vWF returned to normal levels. The increase in thrombin generation and activity pointed to a persistent hypercoagulable state 2 years after MI. Plasma levels of TF and TFPI showed no statistically significant variations with respect to the normal values over the 2-year period studied. In conclusion, these results suggest a persistent generation and activity of thrombin and cellular activation in these patients after MI.","PeriodicalId":12910,"journal":{"name":"Haemostasis","volume":"28 6","pages":"301-6"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000022446","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21325115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Comparative study of a portable monitor for prothrombin time determination, Coaguchek, with three systems for control of oral anticoagulant treatment. 便携式凝血酶原时间测定仪Coaguchek与三种口服抗凝治疗控制系统的比较研究。

Haemostasis Pub Date : 1998-11-01 DOI: 10.1159/000022449

M Vacas, P J Lafuente, S Cuesta, J A Iriarte

{"title":"Comparative study of a portable monitor for prothrombin time determination, Coaguchek, with three systems for control of oral anticoagulant treatment.","authors":"M Vacas, P J Lafuente, S Cuesta, J A Iriarte","doi":"10.1159/000022449","DOIUrl":"https://doi.org/10.1159/000022449","url":null,"abstract":"Self-testing of oral anticoagulation is a new possibility related to the development of portable capillary whole blood prothrombin time monitors. The aim of this study was to evaluate one of this monitors, Coaguchek, with respect to its comparability with our routine prothrombin time determination system, as well as with the reference manual technique and two thromboplastins of high sensitivity, Manchester Reagent and one manufactured in our center, Thromboplastin Bilbao, in a group of patients on oral anticoagulant treatment. Although a correlation of r = 0.9271 was found between international normalized ratio (INR) values of Coaguchek and our routine method, Neoplastine/STA analyzer, the difference of the INR scatter increased with the magnitude of measurement, being lowest for INR between the portable monitor and Manchester Reagent and Thromboplastin Bilbao, with a similar coefficient of correlation, r = 0.8948 and r = 0.8905, respectively. A test was performed showing a 65.6% agreement with the INR values of the STA analyzer, 66.4% with Manchester Reagent and 73.4% with Thromboplastin Bilbao. On the basis of this correspondence with laboratory prothrombin time results Coaguchek may be considered as a possible option for monitoring anticoagulated patients even though patients should be given instructions and advice as regards the management and interpretation of the results.","PeriodicalId":12910,"journal":{"name":"Haemostasis","volume":"28 6","pages":"321-8"},"PeriodicalIF":0.0,"publicationDate":"1998-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000022449","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21325117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Other Uses of LMWHs - Miscellaneous. lmwh的其他用途-杂项。

Haemostasis Pub Date : 1998-11-01

引用次数: 0