Heliyon最新文献

{"title":"Performance analysis of steel W18CR4V grinding using RSM, DNN-GA, KNN, LM, DT, SVM models, and optimization via desirability function and MOGWO.","authors":"Sofiane Touati, Haithem Boumediri, Yacine Karmi, Mourad Chitour, Khaled Boumediri, Amina Zemmouri, Athmani Moussa, Filipe Fernandes","doi":"10.1016/j.heliyon.2025.e42640","DOIUrl":"10.1016/j.heliyon.2025.e42640","url":null,"abstract":"<p><p>This study presents an innovative approach to optimizing the grinding process of W18CR4V steel, a high-performance material used in reamer manufacturing, using advanced machine learning models and multi-objective optimization techniques. The novel combination of Deep Neural Networks with Genetic Algorithm (DNN-GA), K-Nearest Neighbors (KNN), Levenberg-Marquardt (LM), Decision Trees (DT), and Support Vector Machines (SVM) was employed to predict key process outcomes, such as surface roughness (Ra), maximum roughness height (Rz), and production time. The results reveal significant improvements, with Ra values ranging from 0.231 μm to 1.250 μm (up to 81.5 % reduction) and Rz from 1.519 μm to 6.833 μm (up to 77.7 % reduction). The hybrid DNN-GA model achieved R² > 0.99, reducing prediction errors by 23-45 % compared to traditional models. Optimization via the Desirability Function achieved Ra values around 0.341 μm and Rz around 2.3 μm, with production times ranging from 1181 to 1426 s. The innovative Multi-Objective Grey Wolf Optimization (MOGWO) provided Pareto-optimal solutions, minimizing Ra to 0.3 μm, Rz to 1.5 μm, and production times between 2000 and 3000 s, offering better balance between surface quality and machining efficiency. This work highlights the unique integration of machine learning models with optimization techniques to significantly enhance grinding performance and manufacturing efficiency in high-precision industries.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42640"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the addition of Au and Pt to a Fe-Mn-Si alloy for biodegradable implants.","authors":"J N Lemke, J Fiocchi, C A Biffi, A Coda, A Tuissi","doi":"10.1016/j.heliyon.2025.e42663","DOIUrl":"10.1016/j.heliyon.2025.e42663","url":null,"abstract":"<p><p>Fe-Mn based alloys are particular promising for the development of temporary bioabsorbable implants. They exhibit good performance in biological tests, improved mechanical properties and more adequate degradation rates than pure iron for the targeted application. In addition, this system possesses an excellent processability, making it particular suitable for designing thin structures and tailoring the chemistry by alloying. Accordingly, earlier works indicated that by adding Si to Fe-Mn, mechanical properties and long-term degradation behaviour could be improved. This study builds up on the alloying approach adding a fourth noble element to further enhance degradation rate, strain-hardening performance and to pave the way for preparing functionally optimized implant materials as Pt and Au can increase radiopacity and their ions are potentially antibacterial. The alloys were prepared by arc-melting and processed into sheets. Dissolution behaviour was measured by electro-chemical corrosion and static degradation set-up, mechanical properties were studied in tensile mode. Particular emphasis is placed on the different evolution of microstructure in these alloys after rolling and its impact on passivation and degradation. This study demonstrates that quaternary Fe-Mn-Si-(Pt, Au) alloys can be prepared successfully, further accelerating degradation in comparison with ternary alloys.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42663"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing gold nanoparticles in plasmonic photothermal therapy for cancer treatment.","authors":"Amina Badir, Siham Refki, Zouheir Sekkat","doi":"10.1016/j.heliyon.2025.e42738","DOIUrl":"10.1016/j.heliyon.2025.e42738","url":null,"abstract":"<p><p>In recent decades, significant attention has been directed towards gold nanoparticles due to their exceptional properties, capturing the interest of researchers globally. Their unique characteristics, such as localized surface plasmon resonance, high surface area to volume ratio, biocompatibility, and facile surface functionalization, render them highly suitable for diverse applications, ranging from optoelectronics and sensing to surface-enhanced spectroscopies and biomedical uses, particularly in the realm of photothermal therapy. Plasmonic photothermal therapy, an emerging biomedical technology, has garnered substantial interest for its potential in cancer treatment and management. This approach employs photothermal agents, such as gold nanoparticles, which absorb light in the near-infrared region. When these agents accumulate within cancer cells, the absorbed photon energy is converted into heat, inducing local hyperthermia. This localized effect selectively eliminates damaged cells adjacent to nanoparticles while sparing normal cells. Various shapes and sizes of gold nanoparticles have proven well-suited candidates for photothermal therapy. This paper provides an overview of the distinctive properties of gold nanoparticles. It delves into the surface functionalization techniques crucial for ensuring cancer cells' effective retention and targeting of gold nanoparticles. In this context, the present paper reviews diverse applications of gold nanoparticles with different shapes in plasmonic photothermal therapy, encompassing nanospheres, nanorods, nanoshells, nanostars, and nanocages.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42738"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effects of saffron compounds on multiple sclerosis: Molecular docking and dynamic, enhancing properties with gold nanoparticles and evaluating antioxidant, antibacterial, cytotoxicity.","authors":"Shaghayegh Mohammadi, Hamed Farjam, Sharieh Hosseini, Kambiz Larijani","doi":"10.1016/j.heliyon.2025.e42693","DOIUrl":"10.1016/j.heliyon.2025.e42693","url":null,"abstract":"<p><p>The unique properties of nanoparticles, such as their small size and the ability to cross the blood-brain barrier, make them well-suited for targeted drug delivery to the central nervous system. Ongoing research is dedicated to developing innovative nanoparticle-based therapies for a neurological disorder; multiple sclerosis (MS). The study confirmed green synthesis as the optimal method for producing environmentally friendly compounds. The nanoparticles were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD), and transmission electron microscopy (TEM). To evaluate the antioxidant properties, we employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, while conducting a comprehensive examination of antibacterial activity against both Gram-negative (<i>E. coli</i>) and Gram-positive bacteria (<i>S. aureus</i>). Moreover, we performed a thorough assessment of cellular toxicity using saffron extracts and nanoparticles on C6 and SH-SY5Y cells. We employed molecular docking computational methods to investigate the potential inhibitory effects of natural compounds derived from saffron on Multiple Sclerosis. The results of biological activities and computational modeling show that gold nanoparticles improve the performance of plant compounds and also these compounds can be involved in inhibiting proteins responsible for multiple sclerosis.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42693"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manganese mineralization in the northern Middle Atlas (Aderj mining zone, Morocco): Petro-structural and geochemical analysis.","authors":"Mohammed Laiche, Saïd Hinaje, Youssef Drissi, Driss Yaagoub, Mohamed El Fartati, Youssef Ouahzizi, Anas Laksir","doi":"10.1016/j.heliyon.2025.e42650","DOIUrl":"10.1016/j.heliyon.2025.e42650","url":null,"abstract":"<p><p>The Aderj area, part of the northern Middle Atlas of Morocco, is characterized by significant manganese oxide mining potential. These are associated with calcite components and iron oxides mainly hosted by Upper Triassic doleritic basalts, Liassic flint limestones and Vallesian (Middle-Upper Tortonian) lacustrine formations. However, the petrographic and mineralogical characteristics of this mineralization, along with its origin and emplacement mode, remain poorly defined in the study area. Addressing these gaps is essential for assessing the mining and economic potential of this region. This paper involves a meticulous field work approach, including detailed geological surveys of the different host series, as well as careful analysis of the mineralogical typology and morphology. Moreover, the petrography, mineralogy and geochemistry of these Mn(-Fe) mineralization allows to characterize the composition and geochemical features in order to propose an emplacement model of the Mn mineralization, which haves never been the subject of previous investigation. X-ray diffraction combined with light and scanning electron microscopy reveal a polyphase manganese and iron oxide sequences mainly formed from Mn²⁺ rich-hydrothermal fluids derived from leaching and hydrothermal alteration of ferromagnesian minerals from Upper Triassic basalt flows. This sequence is also enriched in geochemical elements such as Pb, Zn, and Ba resulting from the alteration of K-feldspar, probably derived from deep magmatic rocks. Fluids flow along the fractures and faults which affect this area, and deposited mineralization in fracture zones as veins and veinlets during their ascent and mixing with low-temperature, well-oxygenated surficial waters.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42650"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of bio-fertilizer replacement with chemical fertilizer in sesame (<i>Sesamum indicum</i> L) production under drought stress and normal irrigation condition.","authors":"Nasser Nourzadeh, Asghar Rahimi, Amir Dadrasi","doi":"10.1016/j.heliyon.2025.e42743","DOIUrl":"10.1016/j.heliyon.2025.e42743","url":null,"abstract":"<p><p>Drought stress represents a considerable environmental challenge, exerting a deleterious effect on plant growth and productivity. In order to address this issue, the use of biostimulants, such as plant growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF), has gained increasing attention in recent years. The present study, conducted in 2022, sought to evaluate the effects of biological and chemical fertilizers under drought-stress conditions on sesame yield and water-use efficiency. The research was conducted at two farms, Dashthouz and Sarkahnan, which are located approximately 80 km apart in Rodan city, Hormozgan province, Iran. The research was designed as a factorial experiment using a randomized complete block design (RCBD) with three replications. The study examined two main factors: fertilizer application, with eight levels (bacteria (B), mycorrhizal fungi (MY), chemical fertilizer (NPK), bacteria + mycorrhizal fungi (B+MY), bacteria + chemical fertilizer (B+NPK), mycorrhizal fungi + chemical fertilizer (MY+NPK), bacteria + mycorrhizal fungi + chemical fertilizer (B+MY+NPK), and a control), and drought stress, with two levels (normal irrigation without drought stress and drought stress). The results indicated that the main effects of location, irrigation, fertilizer application, and their interactions significantly influenced the leaf area index (LAI), number of branches, number of capsules, number of seeds per capsule, seed yield, biological yield, harvest index, oil yield, meal yield, and water-use efficiency. However, there was no significant effect on thousand-seed weight. This indicates that all measured traits were influenced by the experimental factors. Regarding seed yield, the lowest value of 95.3 g/m² was recorded in the control treatment under normal irrigation conditions at Dachthouz, while the highest value of 325.5 g/m² was achieved in the control treatment under normal irrigation conditions at Sarkahnan. The findings revealed that the application of mycorrhizal fungi (MY) and bacteria (B) as substitutes for phosphorus and nitrogen, respectively, produced seed yields comparable to those achieved with NPK fertilizers under normal irrigation conditions. However, under drought stress conditions, water scarcity disrupted the symbiotic interactions between the microorganisms and the crop, reducing the effectiveness of MY and B treatments in enhancing crop growth and yield. These results contribute to advancing sustainable sesame production systems by minimizing the reliance on chemical fertilizers and enhancing crop resilience to drought stress. Further research and practical implementation of these strategies could lead to more efficient and environmentally sustainable sesame cultivation practices.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42743"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity and biocompatibility of alpha-silver tungstate nanoparticles.","authors":"Sarah Raquel de Annunzio, Bruna de Lima Moraes, Marcelo Assis, Paula Aboud Barbugli, Vinícius Henrique Ferreira Pereira de Oliveira, Elson Longo, Carlos Eduardo Vergani","doi":"10.1016/j.heliyon.2025.e42648","DOIUrl":"10.1016/j.heliyon.2025.e42648","url":null,"abstract":"<p><p>The growing global threat posed by microorganisms resistant to conventional antimicrobials underscores the urgent need for novel agents to control infections. The aim of this study was to evaluate the antimicrobial activity and biocompatibility of alpha-silver tungstate (α-Ag₂WO₄) nanoparticles (NPs) synthesized by the ultrasonic method. The NPs were characterized, and their antimicrobial activity was assessed against <i>Candida albicans</i>, <i>Staphylococcus aureus</i>, and <i>Escherichia coli</i> using the broth microdilution method, determining the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). Intracellular reactive oxygen species (ROS) production was detected by fluorescence using the CM-H₂DCFDA probe. Cytotoxicity was evaluated using murine L929 fibroblasts by MTT assay. Cell viability of both microorganisms and L929 fibroblasts was further assessed using Confocal Laser Scanning Fluorescence Microscopy (CLSM). For <i>C. albicans</i>, the MIC was 3.90 μg/mL, and the MFC was 7.81 μg/mL. For <i>S. aureus</i>, the MIC and MBC were both 62.50 μg/mL, while <i>E. coli</i> exhibited MIC and MBC values of 0.48 μg/mL. The biocompatibility assay revealed a significant reduction in cell viability at concentrations starting from 15.62 μg/mL. CLSM images corroborated the results from both microbiological and biocompatibility assays. Additionally, ROS production was detected in all three microorganisms upon exposure to the NPs, confirming their antimicrobial mechanism. In conclusion, α-Ag₂WO₄ NPs effectively inactivated <i>C. albicans</i>, <i>S. aureus</i>, and <i>E. coli</i>. However, a higher concentration was required to inhibit <i>S. aureus</i> compared to <i>E. coli</i> and <i>C. albicans</i>. The biocompatibility assay revealed concentration-dependent cytotoxic effects. These findings highlight the potential of α-Ag₂WO₄ NPs as antimicrobial agents and suggest further research into their efficacy against biofilms, optimization of their biocompatibility, and the application of these nanomaterials in the incorporation and coating of materials used in biomedical and dental devices.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42648"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of gold nanoparticle-based colorimetric sensing assay functionalized with L-cysteine for the detection of calcium carbide in ripened mango and banana fruits.","authors":"Aishwarya Dixit, Sonam Sonwal, Ashutosh Upadhyay, Vivek K Bajpai, Yun Suk Huh, Shruti Shukla","doi":"10.1016/j.heliyon.2025.e42614","DOIUrl":"10.1016/j.heliyon.2025.e42614","url":null,"abstract":"<p><p>Calcium carbide (CaC₂) is generally used as an artificial ripening agent in the agricultural and food sectors but has been prohibited due to its negative impacts on the environment and human. Therefore, in the present study, a novel and cost-effective detection technique was developed for the detection of CaC₂ using L-cysteine functionalized gold nanoparticles (AuNPs). In this work, a rapid, simple, environment friendly and portable colorimetric nanosensing assay was developed using two different sizes of L-cysteine functionalized gold nanoparticles (AuNP₂₀ and AuNP₄₀). The sensing strategy relied on the fact that the wherein introduction of CaC₂ initiates aggregation, resulting in detectable color change (red to purple) that helps in detection of CaC₂. The efficiency of the developed sensor was investigated on the artificially ripened mangoes and bananas for selective detection of CaC_2. The characterization of synthesized AuNPs was done using Uv-vis spectroscopy, FTIR, particle size, HR-TEM, and XRD analysis. The developed and optimized nanosensing assay was effectively utilized to detect the presences of CaC₂ in carbide treated mango and banana fruits. The 20 nm and 40 nm size and stability (-32.6 mV for 20 nm and -27 mV for 40 nm) of the developed AuNPs were confirmed by TEM analysis, particle size and zeta potential analysis. The application of gold nanoparticle-based sensing assay confirms the presence of CaC₂ in carbide treated mango and banana fruits by turning their color from red to purple. The developed nanosensing assay showed good sensitivity and selectivity towards CaC₂ with LOD of 5 × 10^-3 mL^-1, reinforcing the suggestion that, this sensing assay enables rapid detection of CaC₂ in fruit samples without need of a complicated laboratory setup. This simple detection method has the potential to ensure food safety, public health concerns, international standards for fruits exports, and promotes sustainable ripening methods.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42614"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti photoaging mechanism of a novel recombinant human fibronectin peptide (rhFNP) derived from the extracellular matrix.","authors":"Yuan Wang, Qirong Zhang, Hui Liao, Xiangyi Lu, Jianhang Cong, Zhao Liu, Qin Liu, Chaoqing Deng, Yong Cheng, Peng Shu, Qi Xiang","doi":"10.1016/j.heliyon.2025.e42730","DOIUrl":"10.1016/j.heliyon.2025.e42730","url":null,"abstract":"<p><p>Photoaging is a major cause of skin ageing. Ultraviolet radiation is a key factor in this process, as it can increase the expression of inflammatory factors, promote an inflammatory response, and disrupt the balance between extracellular matrix (ECM) synthesis and degradation, resulting in impaired skin barrier function and increased skin sensitivity. Therefore, anti-inflammation and ECM remodelling are important directions in skin anti-aging research. To simulate the ECM, we constructed a novel recombinant human fibronectin peptide (rhFNP) using synthetic biology. HaCaT, P815, and NHEK cells, Zebrafish, and a KM mouse photoaging model, along with transcriptomics analysis, were used to test rhFNP function and effects. rhFNP mediated HaCaT cell adhesion and migration (<i>p</i> < 0.05), inhibited MMPs gene expression and increased protein expression of Col1α1, Col3α1, and LOR in the NHEK photoaging model, and promoted gene expression of FLG and LOR in Zebrafish (<i>p</i> < 0.01). Moreover, rhFNP inhibited P815 degranulation induced by C48/80 (<i>p</i> < 0.001) and RAW264.7 secretion of TNF-α induced by Lipopolysaccharide (LPS) (<i>p</i> < 0.001). In the KM mouse photoaging model, rhFNP reduced skin damage, ameliorated redness in the sixth week, reduced abnormal thickening of the skin epidermis and dermis, and increased the skin collagen content (<i>p</i> < 0.01, vs. Modol). Transcriptomics analysis showed rhFNP may reduce IL-1α, IL-1β, and IL-18 through immune response, inhibit metalloproteinases, and promote ECM synthesis gene expression.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42730"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of GABA/BDZ receptors in the anticonvulsant effects of dihydrosanguinarine from <i>Bocconia arborea</i> S. Watson.","authors":"José David González-Gómez, Samuel Estrada-Soto, Emmanuel Gaona-Tovar, María Eva González-Trujano, Benjamín Villasana-Salazar, Hugo Fernando Narváez-González, David Martínez-Vargas","doi":"10.1016/j.heliyon.2025.e42701","DOIUrl":"10.1016/j.heliyon.2025.e42701","url":null,"abstract":"<p><p><i>Bocconia arborea</i> S. Watson (<i>B. arborea</i>) belongs to the Papaveraceae family and is used in folk medicine because of its depressant effect on the central nervous system. However, its benefits on cerebral paroxysmal activity as an anticonvulsant treatment remain unknown. This study aimed to evaluate the anticonvulsant effect of <i>B. arborea</i> and one of its abundant isolated metabolites dihydrosanguinarine (DHS) by means of experimental models of seizures in mice simultaneously to an electrocorticographic (ECoG) analysis. <i>B. arborea</i> was prepared as extracts with different polarity using hexane (HEX), dichloromethane (DCM), and methanol (MeOH) solvents to choose the most active one and to identify and isolate a bioactive metabolite. DHS was purified from the HEX extract. The anticonvulsant activity was evaluated with each extract giving an intraperitoneal (i.p.) administration of 100 mg/kg, and 30 min after, seizure behavior was induced by the pentylenetetrazole (PTZ, 85 mg/kg, i.p.) or maximal electroshock stimulation (MES) tests in mice. Molecular docking analysis was performance to reinforce the possible mechanism of action. Our results of seizure behavior and ECoG analysis showed that the HEX extract significantly (p < 0.0001) delayed the onset of seizures and decreased the incidence (p < 0.005) with lower mortality rate (p < 0.05), where DHS was partially responsible for this activity with a possible GABAergic mechanism of action involving the BDZ allosteric site. The MeOH extract also produced a significant effect (p < 0.05) but with less intensity than the HEX extract in the PTZ-induced seizures. A similar anticonvulsant response was observed in the MES test in the presence of DHS (p < 0.005) and the HEX extract (p < 0.05), but not in the case of the MeOH extract. In conclusion, our study provides evidence that non-polar and polar constituents of <i>B. arborea</i> participate in its anticonvulsant property, where DHS might play an important role in the therapy of convulsions mediated by GABA_A/BDZ receptor modulation.</p>","PeriodicalId":12894,"journal":{"name":"Heliyon","volume":"11 4","pages":"e42701"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}