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Evaluation of reagin card tests for syphilis. 评价梅毒的反应卡试验。
Health laboratory science Pub Date : 1978-04-01
R W Stevens, K Gombel, H A Gaafar
{"title":"Evaluation of reagin card tests for syphilis.","authors":"R W Stevens,&nbsp;K Gombel,&nbsp;H A Gaafar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The macroscopic rapid plasma reagin (RPR) and reagin screen (RST) card tests for syphilis were compared. Test specimens were also examined with the Venereal Disease Research Laboratory (VDRL) microscopic slide test; those reactive in the card or slide tests were examined with the fluorescent treponemal antibody-absorption (FTA-ABS) procedure. Agreement between the card tests with 2,300 specimens was 99%; among all three reagin tests it was 96.3%. With 348 specimens reactive in one or more of the tests, there was 75.6% agreement among all three tests. The FTA-ABS was reactive with 71% of the RPR- or RST- and 65% of the VDRL-reactive specimens. The comparison showed that the card tests give results in close agreement with those of VDRL while offering a number of practical advantages over it.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 2","pages":"81-5"},"PeriodicalIF":0.0,"publicationDate":"1978-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11565451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple method for estimating a 'heparin binding capacity' of human serum. 估算人血清“肝素结合能力”的简单方法。
Health laboratory science Pub Date : 1978-01-01
R D Pearson, A H Lubin
{"title":"A simple method for estimating a 'heparin binding capacity' of human serum.","authors":"R D Pearson,&nbsp;A H Lubin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method for estimating a 'heparin binding capacity' of human serum is described. When human serum is diluted with a low ionic strength, heparin-containing buffer at pH 5.5, protein-heparin electrostatic complexes form in solution with subsequent formation of insoluble aggregates which can be collected by centrifugation. Quantitative determination of the relative amounts by weight of protein and heparin in the insoluble heparin-protein aggregates permits estimation of a combining ratio at which serum proteins bind with heparin to precipitate from solution. This weight combining ratio of protein and heparin is a quantitative measure of the total affinity for heparin of all proteins in serum which bind heparin at pH 5.57 to form an insoluble complex. An unusually high affinity for heparin by an abnormal serum protein or an increase in amount of a normally-occuring, high heparin-affinity, serum protein would alter the average protein: heparin combining ratio and increase the 'heparin-binding capacity' of human serum. The converse would be true for serum proteins having a low affinity for heparin, lowering the 'heparin-binding capacity' of human serum. The described method was used to evaluate the 'heparin-binding capacity' of serum proteins in normal individuals and in persons with cystic fibrosis.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 1","pages":"32-7"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11842747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Laboratory-acquired infections at the National Animal Disease Center 1960--1976. 1960- 1976年国家动物疾病中心实验室获得性感染
Health laboratory science Pub Date : 1978-01-01
J F Sullivan, J R Songer, I E Estrem
{"title":"Laboratory-acquired infections at the National Animal Disease Center 1960--1976.","authors":"J F Sullivan,&nbsp;J R Songer,&nbsp;I E Estrem","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Experience with exposure to, or infection with pathogenic agents at the National Animal Disease Center is summarized. A total of 60 laboratory-associated exposures to infectious disease agents were reported. Forty-nine exposures resulted from known accidents, but the other 11 were identified only after the development of clinical or serological manifestations of infection. Eighteen cases of laboratory-acquired infections were reviewed. Brucellosis, the most frequently reported laboratory-acquired infection, accounted for one-half of the cases summarized. Three cases of leptospirosis, two cases of Newcastle disease, two cases of ringworm, and a single infection with Mycobacterium bovis and with Salmonella arizonae were also encountered. The most frequently reported causes of exposure were: auto-inoculation or spray exposure associated with the use of the hypodermic syringe, cuts or lacerations, direct contact with infected animals, and mouth pipetting. Although the infecting event could not be identified in 11 infections, presumptive evidence suggests aerogenic transmission as a probable route of exposure in a number of such cases.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 1","pages":"58-64"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11768297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrhoeae from clinical specimens. 万古霉素和林可霉素在NYC培养基中对临床标本淋病奈瑟菌的选择性研究。
Health laboratory science Pub Date : 1978-01-01
Y C Faur, M H Weisburd, M E Wilson
{"title":"The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrhoeae from clinical specimens.","authors":"Y C Faur,&nbsp;M H Weisburd,&nbsp;M E Wilson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Varying concentrations of vancomycin and lincomycin were tested separately in NYC medium to determine the degree of their selectivity and sensitivity for isolation of N. gonorrhoeae from clinical specimens. Results indicate that 2 microgram/ml of vancomycin used in conjunction with colymycin, amphotericin B, and trimethoprim lactate provide adequate selectivity and reduce the possibility of losses of vancomycin-sensitive strains of gonococci seen with 3 microgram/ml of vancomycin. The same medium with concentrations of from 1 to 4 microgram/ml of lincomycin substituted for vancomycin permitted more contamination and fewer recoveries of gonococci as compared with 2 microgram/ml of vancomycin.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 1","pages":"22-7"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11623368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pseudomonas aeruginosa in swimming pools related to the incidence of otitis externa infection. 游泳池中铜绿假单胞菌与外耳感染发生率的关系。
Health laboratory science Pub Date : 1978-01-01
P L Seyfried, D J Fraser
{"title":"Pseudomonas aeruginosa in swimming pools related to the incidence of otitis externa infection.","authors":"P L Seyfried,&nbsp;D J Fraser","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A study is reported which demonstrates that four out of twenty-four people developed otitis externa as a result of swimming in a pool with a high incidence of Pseudomonas aeruginosa. Serotyping and phage typing were used to establish that P. aeruginosa was the etiolgoical event responsible for the outbreaks of the infection.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 1","pages":"50-7"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11623369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzyme linked immunosorbent assay (ELISA) for determination of Staphylococcus aureus enterotoxin type B. 酶联免疫吸附法测定B型金黄色葡萄球菌肠毒素。
Health laboratory science Pub Date : 1978-01-01
S Notermans, H L Verjans, J Bol, M van Schothorst
{"title":"Enzyme linked immunosorbent assay (ELISA) for determination of Staphylococcus aureus enterotoxin type B.","authors":"S Notermans,&nbsp;H L Verjans,&nbsp;J Bol,&nbsp;M van Schothorst","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Determination of Staphylococcus aureus enterotoxin type B using ELISA is described. The ELISA is more sensitive than the micro-slide technique. The specificity of the determination of enterotoxin B depends on the specificity of the antisera used. Application of this new assay is evaluated and discussed.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 1","pages":"28-31"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11556817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of antibodies to Neisseria gonorrhoeae by counterimmunoelectrophoresis. 淋病奈瑟菌抗体的反免疫电泳检测。
Health laboratory science Pub Date : 1978-01-01
J A Schrader, H A Gaafar
{"title":"Detection of antibodies to Neisseria gonorrhoeae by counterimmunoelectrophoresis.","authors":"J A Schrader,&nbsp;H A Gaafar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antigen was partially purified from Neisseria gonorrhoeae B370 saline wash and used to assay human sera for the presence of antibodies to N. gonorrhoeae. The antigen activity as monitored by counterimmunoelectrophoresis (CIE) is resistant to trypsin and papain but sensitive to heat and periodate oxidation. Of the sera from patients with bacteriologically confirmed cases of gonorrhea, 80% were positive by CIE using this antigen preparation. Of the sera in the negative control group 11% were reactive.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 1","pages":"15-21"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11623367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Copper/zinc ratios in whole blood, plasma, and erythrocytes in pulmonary tuberculosis. 肺结核患者全血、血浆和红细胞中铜/锌比值。
Health laboratory science Pub Date : 1978-01-01
J D Bogden, D I Lintz, M M Joselow, J Charles, J S Salaki
{"title":"Copper/zinc ratios in whole blood, plasma, and erythrocytes in pulmonary tuberculosis.","authors":"J D Bogden,&nbsp;D I Lintz,&nbsp;M M Joselow,&nbsp;J Charles,&nbsp;J S Salaki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Concentrations of copper and zinc in plasma, whole blood, and erythrocytes were determined in pulmonary tuberculosis (TB) patients and controls by atomic absorption spectrophotometry. The plasma and whole blood copper/zinc ratios were markedly higher in the TB patients. Eighty-seven percent of the TB patients had plasma copper/zinc ratios above 2.00, and 94% had whole blood copper/zinc ratios above 0.20. None of the controls had plasma copper/zinc ratios above 2.00, while 24% of the controls had whole blood ratios greater than 0.20. Whole blood and plasma copper/zinc ratios appear to be more sensitive indicators of the presence of pulmonary TB than the individual copper and zinc concentrations. Patients with conditions in which copper and zinc metabolism are altered might benefit from the monitoring of copper/zinc ratios.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 1","pages":"38-43"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11842748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the Minitek test system with a conventional screening procedure for identification of Enterobacteriaceae. Minitek测试系统与常规筛选程序鉴定肠杆菌科的比较。
Health laboratory science Pub Date : 1978-01-01
C W Hanson, E Marso, W J Martin
{"title":"Comparison of the Minitek test system with a conventional screening procedure for identification of Enterobacteriaceae.","authors":"C W Hanson,&nbsp;E Marso,&nbsp;W J Martin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A Minitek basic 9 disc/11 test system plus arabinose, inositol and rhamnose was compared with a conventional six tube screening system for identification of members of the family Enterobacteriaceae. Correlation varied from 88.3 to 100% with the various substrates when studied against 737 strains of these and other gram-negative bacteria recently isolated in our clinical laboratory. Two discs, citrate and ornithine, which originally gave a correlation of 88.3 and 95.5% respectively were modified so that a final correlation of 99.4 and 96.3% was obtained. The average correlation using the eleven discs was 97%.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 1","pages":"3-8"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11556818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Micromethod for rapid identification of gram-negative, nonfermentative bacteria. 快速鉴定革兰氏阴性非发酵细菌的微法。
Health laboratory science Pub Date : 1978-01-01
J B Gibson, S L Crull, K A Borchardt
{"title":"Micromethod for rapid identification of gram-negative, nonfermentative bacteria.","authors":"J B Gibson,&nbsp;S L Crull,&nbsp;K A Borchardt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Because of the contemporary significance of gram-negative, nonfermentative bacteria as etiological agents, a simple, rapid determinative system is essential. Therefore, an accurate, reproducible, and an inexpensive micromethod for the identification of these organisms has been developed. Included in this system are twenty-five biochemical tests. Carbohydrate utilization is demonstrated by modification of Otto and Pickett's formula for oxidative attack and assimilation of carbon sources, while the other substrates are modifications of commercially available products. Inoculation is a two-fold procedure into a plastic multicompartmented tray with wells containing 100 micro liters of each substrate. Initial inoculation yields 10(5) colony forming units per well. The carbohydrates are supplemented with an additional 50 micro liters of a 1 X 10(11) saline suspension of organisms. Reactions are read after a maximum incubation of 48 hr at 35 C. The results obtained with the identification of 124 strains of nonfermentative bacteria utilizing a conventional media schema as suggested by the Center for Disease Control and the micromethod system demonstrated the high reproducibility and correlation achievable with this rapid and economical microtechnique.</p>","PeriodicalId":12869,"journal":{"name":"Health laboratory science","volume":"15 1","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11556819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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