淋病奈瑟菌抗体的反免疫电泳检测。

Health laboratory science Pub Date : 1978-01-01
J A Schrader, H A Gaafar
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引用次数: 0

摘要

从淋病奈瑟菌B370生理盐水洗液中部分纯化抗原,用于测定人血清中是否存在淋病奈瑟菌抗体。反免疫电泳(CIE)检测的抗原活性对胰蛋白酶和木瓜蛋白酶具有抗性,但对热和高碘酸盐氧化敏感。在细菌学确诊的淋病患者血清中,80%使用该抗原制备的CIE阳性。阴性对照组的血清有11%的反应性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of antibodies to Neisseria gonorrhoeae by counterimmunoelectrophoresis.

Antigen was partially purified from Neisseria gonorrhoeae B370 saline wash and used to assay human sera for the presence of antibodies to N. gonorrhoeae. The antigen activity as monitored by counterimmunoelectrophoresis (CIE) is resistant to trypsin and papain but sensitive to heat and periodate oxidation. Of the sera from patients with bacteriologically confirmed cases of gonorrhea, 80% were positive by CIE using this antigen preparation. Of the sera in the negative control group 11% were reactive.

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