Hereditas最新文献

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Integrated analysis of N6-methyladenosine- and 5-methylcytosine-related long non-coding RNAs for predicting prognosis in cervical cancer 综合分析 N6-甲基腺苷和 5-甲基胞嘧啶相关长非编码 RNA 预测宫颈癌预后
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-09-16 DOI: 10.1186/s41065-024-00336-w
Jie Gao, Xiuling Zhang, Anqi Xu, Wei Li, HaiYan Gao
{"title":"Integrated analysis of N6-methyladenosine- and 5-methylcytosine-related long non-coding RNAs for predicting prognosis in cervical cancer","authors":"Jie Gao, Xiuling Zhang, Anqi Xu, Wei Li, HaiYan Gao","doi":"10.1186/s41065-024-00336-w","DOIUrl":"https://doi.org/10.1186/s41065-024-00336-w","url":null,"abstract":"N6-methyladenosine (m6A) and 5-methylcytosine (m5C) play a role in modifying long non-coding RNAs (lncRNAs) implicated in tumorigenesis and progression. This study was performed to evaluate prognostic value of m6A- and m5C-related lncRNAs and develop an efficient model for prognosis prediction in cervical cancer (CC). Using gene expression data of TCGA set, we identified m6A- and m5C-related lncRNAs. Consensus Clustering Analysis was performed for samples subtyping based on survival-related lncRNAs, followed by analyzing tumor infiltrating immune cells (TIICs). Optimal signature lncRNAs were obtained using lasso Cox regression analysis for constructing a prognostic model and a nomogram to predict prognosis. We built a co-expression network of 23 m6A-related genes, 15 m5C-related genes, and 62 lncRNAs. Based on 9 m6A- and m5C-related lncRNAs significantly associated with overall survival (OS) time, two molecular subtypes were obtained, which had significantly different OS time and fractions of TIICs. A prognostic model based on six m6A- and m5C-related signature lncRNAs was constructed, which could dichotomize patients into two risk subgroups with significantly different OS time. Prognostic power of the model was successfully validated in an independent dataset. We subsequently constructed a nomogram which could accurately predict survival probabilities. Drug sensitivity analysis found preferred chemotherapeutic agents for high and low-risk patients, respectively. Our study reveals that m6A- and m5C-related lncRNAs are associated with prognosis and immune microenvironment of CC. The m6A- and m5C-related six-lncRNA signature may be a useful tool for survival stratification in CC and open new avenues for individualized therapies.","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"16 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142257060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mir-150-5p distinguishes acute pulmonary embolism, predicts the occurrence of pulmonary arterial hypertension, and regulates ox-LDL-induced endothelial cell injury Mir-150-5p 可区分急性肺栓塞、预测肺动脉高压的发生并调节氧化-LDL 诱导的内皮细胞损伤
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-09-10 DOI: 10.1186/s41065-024-00333-z
Yue Wu, Xin Sun, Guangqiang Cui, Shu Wang
{"title":"Mir-150-5p distinguishes acute pulmonary embolism, predicts the occurrence of pulmonary arterial hypertension, and regulates ox-LDL-induced endothelial cell injury","authors":"Yue Wu, Xin Sun, Guangqiang Cui, Shu Wang","doi":"10.1186/s41065-024-00333-z","DOIUrl":"https://doi.org/10.1186/s41065-024-00333-z","url":null,"abstract":"Acute pulmonary embolism (APE) is a major type of venous thromboembolism (VTE) with a high risk of mortality and disability. There is a lack of biomarkers for APE to indicate deteriorating development and predict adverse outcomes. This study evaluated the significance of miR-150-5p in APE aiming to explore a novel potential biomarker for APE. The study enrolled APE (n = 137) and deep wein thrombosis (DVT, n = 67) patients and collected plasma samples from all study subjects. The expression of miR-150-5p was analyzed by PCR and its significance in screening APE and pulmonary arterial hypertension (PAH) was assessed by receiver operating curve (ROC) and logistic analyses. The study established oxidized low-density lipoprotein (ox-LDL)-induced human venous endothelial cells (HUVECs). Through cell transfection combined with cell counting kit-8 (CCK8), flow cytometry, and enzyme-linked immunosorbent assay (ELISA), the effect of miR-150-5p on ox-LDL-induced HUVEC injury was evaluated. Significant downregulation of miR-150-5p was observed in the plasma of APE patients compared with DVT patients (P < 0.0001). The plasma miR-150-5p levels in APE patients occurred PAH was much lower than in patients without PAH (P < 0.0001). Reducing miR-150-5p distinguished APE patients from DVT patients (AUC = 0.912) and was identified as a risk factor for the occurrence of PAH in APE patients (OR = 0.385, P = 0.010). In HUVECs, oxidized low-density lipoprotein (ox-LDL) caused inhibited cell proliferation, enhanced apoptosis, increased pro-inflammatory cytokines, reactive oxygen species (ROS), malondialdehyde (MDA), and decreased superoxide dismutase (SOD). Overexpressing miR-150-5p could promote proliferation, inhibit apoptosis, and alleviate inflammation and oxidative stress of ox-LDL-treated HUVECs. Downregulated plasma miR-150-5p served as a diagnostic biomarker for APE and predicted the predisposition of PAH in APE patients. Overexpressing miR-150-5p could alleviate ox-LDL-induced endothelial cell injury in HUVECs.","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"34 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142219373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the molecular mechanism of ginseng against anthracycline-induced cardiotoxicity based on network pharmacology, molecular docking and molecular dynamics simulation. 基于网络药理学、分子对接和分子动力学模拟探索人参抗蒽环类药物诱导的心脏毒性的分子机制
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-09-06 DOI: 10.1186/s41065-024-00334-y
Lin Xie, Hanze Liu, Ke Zhang, Yijun Pan, Mengyao Chen, Xiangyue Xue, Guoxing Wan
{"title":"Exploring the molecular mechanism of ginseng against anthracycline-induced cardiotoxicity based on network pharmacology, molecular docking and molecular dynamics simulation.","authors":"Lin Xie, Hanze Liu, Ke Zhang, Yijun Pan, Mengyao Chen, Xiangyue Xue, Guoxing Wan","doi":"10.1186/s41065-024-00334-y","DOIUrl":"10.1186/s41065-024-00334-y","url":null,"abstract":"<p><strong>Background: </strong>Previous clinical and basic studies have revealed that ginseng might have cardioprotective properties against anthracycline-induced cardiotoxicity (AIC). However, the underlying mechanism of ginseng action against AIC remains insufficiently understood. The aim of this study was to explore the related targets and pathways of ginseng against AIC using network pharmacology, molecular docking, cellular thermal shift assay (CETSA) and molecular dynamics (MD) simulations.</p><p><strong>Results: </strong>Fourteen drug-disease common targets were identified. Enrichment analysis showed that the AGE-RAGE in diabetic complications, fluid shear stress and atherosclerosis, and TNF signaling pathway were potentially involved in the action of ginseng against AIC. Molecular docking demonstrated that the core components including Kaempferol, beta-Sitosterol, and Fumarine had notable binding activity with the three core targets CCNA2, STAT1, and ICAM1. Furthermore, the stable complex of STAT1 and Kaempferol with favorable affinity was further confirmed by CETSA and MD simulation.</p><p><strong>Conclusions: </strong>This study suggested that ginseng might exert their protective effects against AIC through the derived effector compounds beta-Sitosterol, Kaempferol and Fumarine by targeting CCNA2, STAT1, and ICAM1, and modulating AGE-RAGE in diabetic complications, fluid shear stress and atherosclerosis, and TNF signaling pathways.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"161 1","pages":"31"},"PeriodicalIF":2.7,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11378563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Can Omega-3 prevent the accidence of stroke: a mendelian randomization study. 欧米茄-3 能否预防中风的发生:一项孟德尔随机研究。
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-09-05 DOI: 10.1186/s41065-024-00329-9
Chongcheng Xi, Jie Zhang, Haihui Liu, Sian Tao, Ying Xie, Jibin Liu, Changqing Tong, Dong Tian, Hua Ye, Xiaobo Zhang
{"title":"Can Omega-3 prevent the accidence of stroke: a mendelian randomization study.","authors":"Chongcheng Xi, Jie Zhang, Haihui Liu, Sian Tao, Ying Xie, Jibin Liu, Changqing Tong, Dong Tian, Hua Ye, Xiaobo Zhang","doi":"10.1186/s41065-024-00329-9","DOIUrl":"10.1186/s41065-024-00329-9","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;The lipid-lowering effects of Omega-3 fatty acids have been widely reported, yet their impact on ischemic stroke remains controversial. Reports on the protective effects of unsaturated fatty acids, such as Omega-6 and Omega-7, as well as saturated fatty acids in cardiovascular diseases, including hypertension and ischemic stroke, are less frequent.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;This study aims to identify fatty acids associated with blood pressure and ischemic stroke through Mendelian randomization. Besides, it seeks to determine whether specific fatty acids can prevent ischemic stroke by managing blood pressure and revealing the specific mechanisms of this action.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This research involved downloading relevant data from websites and extracting SNPs that met the standard criteria as instrumental variables. Simultaneously, the 'MR-PRESSO' package and 'Mendelian Randomization' package were used to eliminate confounding SNPs that could bias the study results. Then, inverse variance weighting and the weighted median were employed as primary analysis methods, accompanied by sensitivity analysis to assess the validity of the causal relationships. Initially, multivariable Mendelian randomization was used to identify fatty acids linked to blood pressure and the incidence of ischemic stroke. The causal link between certain fatty acids and the initiation of ischemic stroke was then investigated using bidirectional and mediator Mendelian randomization techniques. Stepwise Regression and the Product of Coefficients Method in mediator Mendelian randomization were utilized to ascertain whether specific fatty acids reduce ischemic stroke risk by lowering blood pressure.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Multivariable Mendelian randomization analysis indicated a potential inverse correlation between Omega-3 intake and both blood pressure and ischemic stroke. Consequently, Omega-3 was selected as the exposure, with blood pressure and ischemic stroke-related data as outcomes, for further bidirectional and mediation Mendelian Randomization analyses. Bidirectional Mendelian Randomization revealed that Omega-3 significantly influences DBP (P = 1.01e-04) and IS (P = 0.016). It also showed that DBP and SBP significantly affect LAS, SVS, CES, IS, and LS. Mediator Mendelian Randomization identified five established mediating pathways: Omega-3-Diastolic blood pressure-Small vessel stroke, Omega-3-Diastolic blood pressure-Cardioembolic stroke, Omega-3-Diastolic blood pressure-Lacunar stroke, Omega-3-Diastolic blood pressure-Large artery atherosclerosis stroke, and Omega-3-Diastolic blood pressure-Ischemic stroke. Of these, four pathways are complete mediation, and one pathway is partial mediation.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The findings suggest that Omega-3 may indirectly reduce the incidence of ischemic stroke by lowering blood pressure. Thus, blood pressure modulation might be one of the mechanisms ","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"161 1","pages":"30"},"PeriodicalIF":2.7,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Combination of arsenic trioxide and apatinib synergistically inhibits small cell lung cancer by down-regulating VEGFR2/mTOR and Akt/c-Myc signaling pathway via GRB10. 三氧化二砷和阿帕替尼通过 GRB10 下调 VEGFR2/mTOR 和 Akt/c-Myc 信号通路,从而协同抑制小细胞肺癌。
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-09-02 DOI: 10.1186/s41065-024-00330-2
Yao Yu, Yu Shang, Si Shi, Yaowu He, Wenchao Shi, Menghan Wang, Qi Wang, Dandan Xu, Ce Shi, Hong Chen
{"title":"Combination of arsenic trioxide and apatinib synergistically inhibits small cell lung cancer by down-regulating VEGFR2/mTOR and Akt/c-Myc signaling pathway via GRB10.","authors":"Yao Yu, Yu Shang, Si Shi, Yaowu He, Wenchao Shi, Menghan Wang, Qi Wang, Dandan Xu, Ce Shi, Hong Chen","doi":"10.1186/s41065-024-00330-2","DOIUrl":"10.1186/s41065-024-00330-2","url":null,"abstract":"<p><strong>Background: </strong>Small cell lung carcinoma (SCLC) is characterized by -poor prognosis, -high predilection for -metastasis, -proliferation, and -absence of newer therapeutic options. Elucidation of newer pathways characterizing the disease may allow for development of targeted therapies and consequently favorable outcomes.</p><p><strong>Methods: </strong>The current study explored the combinatorial action of arsenic trioxide (ATO) and apatinib (APA) in vitro and in vivo. In vitro models were tested using -H446 and -H196 SCLC cell lines. The ability of drugs to reduce -metastasis, -cell proliferation, and -migration were assessed. Using bioinformatic analysis, differentially expressed genes were determined. Gene regulation was assessed using gene knock down models and confirmed using Western blots. The in vivo models were used to confirm the resolution of pathognomic features in the presence of the drugs. Growth factor receptor bound protein (GRB) 10 expression levels of human small cell lung cancer tissues and adjacent tissues were detected by IHC.</p><p><strong>Results: </strong>In combination, ATO and APA were found to significantly reduce -cell proliferation, -migration, and -metastasis in both the cell lines. Cell proliferation was found to be inhibited by activation of Caspase-3, -7 pathway. In the presence of drugs, it was found that expression of GRB10 was stabilized. The silencing of GRB10 was found to negatively regulate the VEGFR2/Akt/mTOR and Akt/GSK-3β/c-Myc signaling pathway. Concurrently, absence of metastasis and reduction of tumor volume were confirmed in vivo. The immunohistochemical results confirmed that the expression level of GRB10 in adjacent tissues was significantly higher than that in human small cell lung cancer tissues.</p><p><strong>Conclusions: </strong>Synergistically, ATO and APA have a more significant impact on inhibiting cell proliferation than each drug independently. ATO and APA may be mediating its action through the stabilization of GRB10 thus acting as a tumor suppressor. We thus, preliminarily report the impact of GRB10 stability as a target for SCLC treatment.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"161 1","pages":"29"},"PeriodicalIF":2.7,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11367874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142119544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correlation between gut microbiota characteristics and non-small cell lung cancer based on macrogenomics sequencing. 基于宏基因组学测序的肠道微生物群特征与非小细胞肺癌之间的相关性。
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-08-27 DOI: 10.1186/s41065-024-00328-w
GuiLin Zeng, LiRong Zeng, Ying Wang, Zhi Cao, XiangHua Zeng, ZhiHong Xue, ShiLan Liu, YaMao Li, Lang He
{"title":"Correlation between gut microbiota characteristics and non-small cell lung cancer based on macrogenomics sequencing.","authors":"GuiLin Zeng, LiRong Zeng, Ying Wang, Zhi Cao, XiangHua Zeng, ZhiHong Xue, ShiLan Liu, YaMao Li, Lang He","doi":"10.1186/s41065-024-00328-w","DOIUrl":"10.1186/s41065-024-00328-w","url":null,"abstract":"<p><strong>Objective: </strong>Non-small cell lung cancer (NSCLC) patients undergoing chemotherapy and immunotherapy experience disturbances in the gut microbiota. This study intends to find out the correlation between gut microbiota and clinical indices before and after radiotherapy for NSCLC.</p><p><strong>Methods: </strong>Ten patients with primary NSCLC were screened, and plasma and fecal samples were collected before and after radiotherapy, respectively. Inflammatory indices in plasma were detected. Genomic DNA was extracted from fecal specimens and sequenced on on Illumina HiSeq2000 sequencing platform. Thee sequenced data were subjected to Metagenome assembly, gene prediction, species annotation, and gene function analysis to study and analyze gut microbiota and metabolic functions. The correlation between the diversity of gut microbiota and the clinical indicators of NSCLC patients was evaluated, and the changes of gut microbiota before and after radiotherapy were observed.</p><p><strong>Results: </strong>The diversity of gut microbiota in NSCLC patients did not correlate with smoking, pathology, and inflammatory markers. The abundance of phylum (p)_Bacteroidetes increased; p_Firmicutes and p_Bacteroidetes accounted for the highest proportion in NSCLC patients, and the abundance of both was dominantly exchanged after radiotherapy. There was a decrease in genus (g)_Bifidobacterium after radiotherapy in NSCLC patients. There was no significant correlation between the diversity of gut microbiota after radiotherapy and radiotherapy sensitivity, and the structural composition and abundance of gut microbiota remained stable.</p><p><strong>Conclusion: </strong>The diversity of gut microbiota is altered after radiotherapy in NSCLC patients, showing an increase in harmful bacteria and a decrease in beneficial bacteria.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"161 1","pages":"26"},"PeriodicalIF":2.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348753/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The number of drones to inseminate a queen with has little potential for optimization of honeybee breeding programs. 为蜂王人工授精的雄蜂数量对蜜蜂育种计划的优化几乎没有影响。
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-08-27 DOI: 10.1186/s41065-024-00332-0
Manuel Du, Richard Bernstein, Andreas Hoppe
{"title":"The number of drones to inseminate a queen with has little potential for optimization of honeybee breeding programs.","authors":"Manuel Du, Richard Bernstein, Andreas Hoppe","doi":"10.1186/s41065-024-00332-0","DOIUrl":"10.1186/s41065-024-00332-0","url":null,"abstract":"<p><strong>Background: </strong>Mating control is a crucial aspect of honeybee breeding. Instrumental insemination of queens gives the breeder maximum control over the genetic origin of the involved drones. However, in addition to the drones' descent, the breeder's control also extends over the number of drones to use for inseminations. Thus far, this aspect has largely been ignored in attempts to optimize honeybee breeding schemes. The literature provides some comparisons between single drone inseminations (SDI) and multi drone inseminations (MDI) but it is unclear whether the number of drones used in MDI is a relevant parameter for the optimization of honeybee breeding programs.</p><p><strong>Methods: </strong>By computer simulations, we investigated the effect of the number of drones per inseminated queen in breeding programs that relied on best linear unbiased prediction (BLUP) breeding values. We covered a range of 1 to 50 drones per queen and observed the developments of genetic gain and inbreeding over a period of 20 years. Hereby, we focused on insemination schemes that take the drones for one queen from a single colony.</p><p><strong>Results: </strong>SDI strategies led to 5.46% to 14.19% higher genetic gain than MDI at the cost of 6.1% to 30.2% higher inbreeding rates. The number of drones used in MDI settings had only a negligible impact on the results. There was a slight tendency that more drones lead to lower genetic gain and lower inbreeding rates but whenever more than five drones were used for inseminations, no significant differences could be observed.</p><p><strong>Conclusion: </strong>The opportunities to optimize breeding schemes via the number of drones used in inseminations are very limited. SDI can be a viable strategy in situations where breeders are interested in genetically homogeneous offspring or precise pedigree information. However, such strategies have to account for the fact that the semen from a single drone is insufficient to fill a queen's spermatheca, whence SDI queens will not build full-strength colonies. When deciding for MDI, breeders should focus on collecting enough semen for a succesful insemination, regardless of how many drones they need for this purpose.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"161 1","pages":"28"},"PeriodicalIF":2.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The novel circFKBP8/miR-432-5p/E2F7 cascade functions as a regulatory network in breast cancer. 新型 circFKBP8/miR-432-5p/E2F7 级联在乳腺癌中发挥着调控网络的作用。
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-08-27 DOI: 10.1186/s41065-024-00331-1
Zhongkui Jin, Wang Xu, Kunlin Yu, Cailu Luo, Xiaodan Luo, Tao Lian, Changshan Liu
{"title":"The novel circFKBP8/miR-432-5p/E2F7 cascade functions as a regulatory network in breast cancer.","authors":"Zhongkui Jin, Wang Xu, Kunlin Yu, Cailu Luo, Xiaodan Luo, Tao Lian, Changshan Liu","doi":"10.1186/s41065-024-00331-1","DOIUrl":"10.1186/s41065-024-00331-1","url":null,"abstract":"<p><strong>Background: </strong>Circular RNAs (circRNAs) are capable of affecting breast cancer (BC) development. However, the role and underneath mechanism of circFKBP8 (also known as hsa_circ_0000915) in BC remain largely unknown.</p><p><strong>Methods: </strong>Expression analyses were performed using quantitative real-time polymerase chain reaction (qRT-PCR), western blot, and immunohistochemistry (IHC) assays. Effects on cell functional phenotypes were determined by assessing cell proliferation, migratory capacity, invasion, and stemness in vitro. The relationship between microRNA (miR)-432-5p and circFKBP8 or E2F transcription factor 7 (E2F7) was examined by RNA pull-down, dual-luciferase reporter, and RNA immunoprecipitation (RIP) assays. Xenograft assays were used to identify the function of circFKBP8 in vivo.</p><p><strong>Results: </strong>CircFKBP8 was presented at high levels in BC tissues and cells. High circFKBP8 expression was associated with worse overall survival in BC patients. CircFKBP8 suppression inhibited BC cell proliferation, migratory capacity, invasion and stemness in vitro. CircFKBP8 suppression blocked xenograft tumor growth in vivo. Mechanistically, circFKBP8 functioned as a miR-432-5p sponge to modulate E2F7 expression. CircFKBP8 modulated BC cell malignant behaviors by miR-432-5p, and miR-432-5p affected these cell phenotypes through E2F7.</p><p><strong>Conclusion: </strong>Our observations prove that circFKBP8 promotes BC malignant phenotypes through the miR-432-5p/E2F7 cascade, offering a promising therapeutic and prognostic target for BC.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"161 1","pages":"27"},"PeriodicalIF":2.7,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11348600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SOLAMEN syndrome with cardiovascular damage. 伴有心血管损伤的 SOLAMEN 综合征。
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-07-30 DOI: 10.1186/s41065-024-00314-2
Xiong Zhao, Xiaojie Yue, Shifan Yuan, Yefeng Dai, Hao Gu
{"title":"SOLAMEN syndrome with cardiovascular damage.","authors":"Xiong Zhao, Xiaojie Yue, Shifan Yuan, Yefeng Dai, Hao Gu","doi":"10.1186/s41065-024-00314-2","DOIUrl":"10.1186/s41065-024-00314-2","url":null,"abstract":"<p><p>SOLAMEN syndrome is a rare, recently recognized congenital syndrome that is characterized by progressive and hypertrophic diseases involving multiple systems, including segmental overgrowth, lipomatosis, arteriovenous malformation (AVM) and epidermal nevus. According to literatures, SOLAMEN syndrome is caused by heterozygous PTEN mutation. Phenotypic overlap complicates the clinical identification of diseases associated with PTEN heterozygous mutations, making the diagnosis of SOLAMEN more challenging. In addition, SOLAMEN often presents with segmental tissue overgrowth and vascular malformations, increasing the possibility of misdiagnosis as klipple-trenaunay syndrome or Parks-Weber syndrome. Here, we present a case of a child presenting with macrocephaly, patchy lymphatic malformation on the right chest, marked subcutaneous varicosities and capillaries involving the whole body, overgrowth of the left lower limb, a liner epidermal nevus on the middle of the right lower limb, and a large AVM on the right cranial thoracic entrance. Based on the typical phenotypes, the child was diagnosed as SOLAMEN syndrome. detailed clinical, imaging and genetic diagnoses of SOLAMEN syndrome was rendered. Next-generation sequencing (NGS) data revealed that except for a germline PTEN mutation, a PDGFRB variant was also identified. A subsequent echocardiographic examination detected potential cardiac defects. We suggested that given the progressive nature of AVM and the potential severity of cardiac damage, regular echocardiographic evaluation, imaging follow-up and appropriate interventional therapy for AVM are recommended.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"161 1","pages":"24"},"PeriodicalIF":2.7,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11287979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription. 与 TBP 和 Exd 组成的新型 Antennapedia 和 Ultrabithorax 三聚体复合物可调控转录。
IF 2.7 3区 生物学
Hereditas Pub Date : 2024-07-30 DOI: 10.1186/s41065-024-00327-x
Alely Villarreal-Puente, Claudia Altamirano-Torres, Gustavo Jiménez-Mejía, Carolina Hernández-Bautista, Rubén Montalvo-Méndez, Martha Vázquez, Mario Zurita, Diana Reséndez-Pérez
{"title":"Novel Antennapedia and Ultrabithorax trimeric complexes with TBP and Exd regulate transcription.","authors":"Alely Villarreal-Puente, Claudia Altamirano-Torres, Gustavo Jiménez-Mejía, Carolina Hernández-Bautista, Rubén Montalvo-Méndez, Martha Vázquez, Mario Zurita, Diana Reséndez-Pérez","doi":"10.1186/s41065-024-00327-x","DOIUrl":"10.1186/s41065-024-00327-x","url":null,"abstract":"<p><strong>Background: </strong>Hox proteins interact with DNA and many other proteins, co-factors, transcriptional factors, chromatin remodeling components, non-coding RNAs and even the extracellular matrix that assembles the Hox complexes. The number of interacting partners continues to grow with diverse components and more transcriptional factors than initially thought. Hox complexes present many activities, but their molecular mechanisms to modulate their target genes remain unsolved.</p><p><strong>Results: </strong>In this paper we showed the protein-protein interaction of Antp with Ubx through the homeodomain using BiFC in Drosophila. Analysis of Antp-deletional mutants showed that AntpHD helixes 1 and 2 are required for the interaction with Ubx. Also, we found a novel interaction of Ubx with TBP, in which the PolyQ domain of TBP is required for the interaction. Moreover, we also detected the formation of two new trimeric complexes of Antp with Ubx, TBP and Exd using BiFC-FRET; these proteins, however, do not form a trimeric interaction with BIP2 or TFIIEβ. The novel trimeric complexes reduced Antp transcriptional activity, indicating that they could confer specificity for repression.</p><p><strong>Conclusions: </strong>Our results increase the number of transcriptional factors in the Antp and Ubx interactomes that form two novel trimeric complexes with TBP and Exd. We also report a new Ubx interaction with TBP. These novel interactions provide important clues of the dynamics of Hox-interacting complexes involved in transcriptional regulation, contributing to better understand Hox function.</p>","PeriodicalId":12862,"journal":{"name":"Hereditas","volume":"161 1","pages":"25"},"PeriodicalIF":2.7,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11290222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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