Clinical significance and biological function of PRKCQ-AS1/miR-582-3p expression in LUAD.

IF 2.5 3区生物学

Hereditas Pub Date : 2025-07-01 DOI:10.1186/s41065-025-00482-9

Lingling Liu, Xiaofen Liu, Xiaojiao Wu, Hang Fang, Jingjing Shi, Wei Jiang

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引用次数: 0

Abstract

Objective: To investigate the clinical value and mechanism of action of long non-coding RNA PRKCQ-AS1 for lung adenocarcinoma (LUAD) progression.

Methods: Clinical data of 128 LUAD patients were collected, postoperative pathological tissues were stored at -80 °C. Kaplan-Meier survival analysis was employed to investigate differences in 5-year survival rates across various expression groups, while Cox regression models assessed the prognostic factors influencing patient outcomes. Reverse transcription quantitative PCR (RT-qPCR) was utilized to measure the expression levels of PRKCQ-AS1 and miR-582-3p in pathological tissues and LUAD cell lines. Additionally, a dual-luciferase reporter assay validated the reciprocal relationship. CCK8 examined cell proliferation, Transwell observed cell migration and invasion.

Results: PRKCQ-AS1 was down-regulated and miR-582-3p was up-regulated in LUAD tissues and cell. PRKCQ-AS1 and miR-582-3p expression affects some pathological features (lymph node metastasis, TNM stage, tumour differentiation) in LUAD patients. Patients with low PRKCQ-AS1 and high miR-582-3p had increased mortality. Interaction of PRKCQ-AS1 targeting miR-582-3p exists in LUAD cells. RGMB, STXBP6 are downstream target genes of miR-582-3p. Overexpression of (oe-) PRKCQ-AS1 inhibited LUAD cell proliferation, migration, and invasion. However, concomitant use of miR-582-3p mimics resisted the effects of PRKCQ-AS1 overexpression on cells.

Conclusion: PRKCQ-AS1/miR-582-3p axis regulatory relationship exists in lung adenocarcinoma cells. PRKCQ-AS1 may regulate the proliferation, migration and invasion of lung adenocarcinoma cells and participate in LUAD regulation by targeting miR-582-3p.

Clinical trial number: Not applicable.

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PRKCQ-AS1/miR-582-3p在LUAD中表达的临床意义及生物学功能

目的：探讨长链非编码RNA PRKCQ-AS1在肺腺癌（LUAD）进展中的临床价值及作用机制。方法：收集128例LUAD患者的临床资料，术后病理组织-80℃保存。Kaplan-Meier生存分析探讨不同表达组5年生存率的差异，Cox回归模型评估影响患者预后的预后因素。采用反转录定量PCR （RT-qPCR）检测PRKCQ-AS1和miR-582-3p在病理组织和LUAD细胞系中的表达水平。此外，双荧光素酶报告试验证实了相互关系。CCK8检测细胞增殖，Transwell观察细胞迁移和侵袭。结果：LUAD组织和细胞中PRKCQ-AS1下调，miR-582-3p上调。PRKCQ-AS1和miR-582-3p的表达影响LUAD患者的一些病理特征（淋巴结转移、TNM分期、肿瘤分化）。低PRKCQ-AS1和高miR-582-3p的患者死亡率增加。在LUAD细胞中存在靶向miR-582-3p的PRKCQ-AS1相互作用。RGMB、STXBP6是miR-582-3p的下游靶基因。（e-） PRKCQ-AS1的过表达抑制LUAD细胞的增殖、迁移和侵袭。然而，同时使用miR-582-3p模拟物可抵抗PRKCQ-AS1过表达对细胞的影响。结论：PRKCQ-AS1/miR-582-3p轴在肺腺癌细胞中存在调控关系。PRKCQ-AS1可能通过靶向miR-582-3p调控肺腺癌细胞的增殖、迁移和侵袭，参与LUAD的调节。临床试验号：不适用。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.