{"title":"Prevalence of Helicobacter pylori infection and effectiveness of first-line triple eradication therapy among dyspeptic patients at hospitals in Hawassa City, Ethiopia: a cross-sectional follow-up study","authors":"Sintayehu Fekadu, Seyife Kibru, Sisay Tesfaye, Tariku Egeno, Alemu Tamiso, Hizkel Engiso, Serawit Deyno","doi":"10.1186/s13099-024-00618-8","DOIUrl":"https://doi.org/10.1186/s13099-024-00618-8","url":null,"abstract":"Dyspepsia is a common gastrointestinal illness sometimes associated with Helicobacter pylori (H. pylori) infection. Screening and eradicating the bacterium reduces the risk of infection-related complications. The aim of this study was to determine the magnitude of H. pylori infection among dyspeptic patients and the effectiveness of triple eradication therapy at hospitals in Hawassa city, Ethiopia. The prevalence of H. pylori infection was 48.5%. The H. pylori eradication rate using first-line triple therapy was 83.8%. Eradication therapy failure is associated with previous exposure compared to no exposure (AOR: 4.8, 95% CI: 1.37–10.97), a regimen for 10-days compared to 14-days (AOR: 4.05, 95% CI: 1.42–11.55), and self-reported side effects compared to no report (AOR: 2.5, 95% CI: 1.12–5.97). Based on Morisky-eight scale 230 (79.0%) patients were adherent to their triple therapy. Participants with no reports of adverse effects showed increased odds of adherence to triple therapy compared to those who had reports (AOR = 2.45, 95% CI: 1.29–4.62). This study demonstrated that about half of adult dyspeptic patients were infected with H. pylori, and moderate eradication was observed. Factors such as previous history of eradication therapy, duration of the eradication regimen, and perception of potential adverse effects are associated with eradication rate and should be considered during the initiation of eradication therapy.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"13 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140801943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gut PathogensPub Date : 2024-04-27DOI: 10.1186/s13099-024-00615-x
Lin Chen, Yue Zhu, Yilin Huang, Keqing Shen, Liying Chen
{"title":"The association between Helicobacter pylori infection and the risk for gout in hyperuricemia patients in China – A cross-sectional study","authors":"Lin Chen, Yue Zhu, Yilin Huang, Keqing Shen, Liying Chen","doi":"10.1186/s13099-024-00615-x","DOIUrl":"https://doi.org/10.1186/s13099-024-00615-x","url":null,"abstract":"Helicobacter pylori (H. pylori) infection has been reported to be associated with multiple metabolic diseases. However, the connection between H. pylori infection and gout has not been explored previously. Our study aimed to investigate the association of gout and H. pylori infection in hyperuricemia population in China. This cross-sectional study was performed among the subjects who underwent health checkup in our health promotion center from January 1, 2020 to December 31, 2021. A total of 53,629 subjects with a mean age of 44.2 years were included in this study. H. pylori infection was defined as a positive [13]C-urea breath test. The effect of H. pylori infection on gout was assessed by multiple logistic regression analysis. 720 subjects with gout and 15,077 subjects with asymptomatic hyperuricemia (> 420 µmol/L in male and > 360 µmol/L in female) were enrolled. The prevalence rates of H. pylori infection, hyperuricemia and gout were 26.3%, 29.5%, 1.3%, respectively. The prevalence rate of H. pylori infection was significantly higher in subjects with gout than in those with asymptomatic hyperuricemia (35.0% vs. 27.2%; P<0.001). Multiple logistic regression analysis showed that H. pylori infection was associated with an increased risk of gout independent of serum uric acid level in hyperuricemia population (odds ratio [OR]: 1.320, 95% confidence interval [CI]: 1.124–1.550, P = 0.001). H. pylori infection is positively associated with higher risk of gout in hyperuricemia population. The causal relationship and potential mechanism between H. pylori infection and gout warrants further investigation.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"4 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140801919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gut PathogensPub Date : 2024-04-10DOI: 10.1186/s13099-024-00599-8
Tintu Varghese, James A. Platts Mills, R. Revathi, Sebastien Antoni, Heidi M. Soeters, Tondo Opute Emmanuel Njambe, Eric R. Houpt, Jacqueline E. Tate, Umesh D. Parashar, Gagandeep Kang
{"title":"Etiology of diarrheal hospitalizations following rotavirus vaccine implementation and association of enteric pathogens with malnutrition among under-five children in India","authors":"Tintu Varghese, James A. Platts Mills, R. Revathi, Sebastien Antoni, Heidi M. Soeters, Tondo Opute Emmanuel Njambe, Eric R. Houpt, Jacqueline E. Tate, Umesh D. Parashar, Gagandeep Kang","doi":"10.1186/s13099-024-00599-8","DOIUrl":"https://doi.org/10.1186/s13099-024-00599-8","url":null,"abstract":"Malnourished children are at higher risk of mortality and morbidity following diarrheal illness and certain enteropathogens have been associated with malnutrition in children. Very few studies have comprehensively looked at the etiology of diarrhea in malnourished children and most have used conventional diagnostic methods with suboptimal sensitivity. We used a highly sensitive molecular approach against a broad range of pathogens causing diarrhea and examined their association with malnutrition. In addition, we looked at the pathogen diversity of pediatric diarrhea, three years after the nationwide rotavirus vaccine introduction to understand the evolving landscape of pathogens, which is crucial for planning strategies to further reduce the diarrhea burden. Clinical details and diarrheal stool samples were collected from hospitalized children aged < 5 years from three sentinel sites in India for a period of one year. The samples were tested by qPCR for 16 established causes of diarrhea using TaqMan Array Cards. A total of 772 children were enrolled, from whom 482 (62.4%) stool specimens were tested. No specific pathogen was associated with diarrhea among children with acute or chronic malnutrition compared to those with better nutritional status. Overall, adenovirus was the leading pathogen (attributable fraction (AF) 16.9%; 95% CI 14.1 to 19.2) followed by rotavirus (AF 12.6%; 95% CI 11.8 to 13.1) and Shigella (AF 10.9%; 95% CI 8.4 to 16.4). The majority of diarrhea requiring hospitalization in children aged < 2 years could be attributed to viruses, while Shigella was the most common pathogen among children aged > 2 years. These data on the prevalence and epidemiology of enteropathogens identified potential pathogens for public health interventions.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"19 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140594290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic evolution and phylogenetic analysis of porcine epidemic diarrhea virus strains circulating in and outside China with reference to a wild type virulent genotype CHYJ130330 reported from Guangdong Province, China","authors":"Mudassar Mohiuddin, Shengchao Deng, Lisai Zhu, Guiping Wang, Aiqing Jia","doi":"10.1186/s13099-024-00597-w","DOIUrl":"https://doi.org/10.1186/s13099-024-00597-w","url":null,"abstract":"During the last decade, porcine epidemic diarrhea virus has detrimental consequences on swine industry, due to severe outbreaks especially in the suckling piglets. In March 2013, an outbreak was reported on a commercial swine farm in Guangdong Province, Southern China. A wild-type PEDV strain named as CHYJ130330 was identified, complete genome was sequenced and deposited in GenBank (accession no. KJ020932). The molecular epidemiological including evolutionary characteristics and pathogenicity assessment were explored during this study with particular interest and focus to develop this candidate strain for new vaccine. The isolates from China pre- and post-2013 shared 96.5–97.2% and 97–99% nt identity respectively with wild-type CHYJ130330 strain which during experimental studies has demonstrated high virulence and 100% mortality in 104 TCID50 group piglets within 5 days. The 22 reference strains selected from other parts of the world shared 98–99% identity with our sequence except Chinese (CV777) and S. Korean (vir.DR13, SM98 and atten.DR13) strains sharing 96.8, 97.6, 96.6 and 97.1% identity respectively. The phylogenetic tree revealed most strains reported after 2013 in GII genogroup while the prototype (CV777), S.korean and earlier Chinese (JS2008, 85-7mutant, Atten.vaccine, SD-M, LZC and CH/S) were GI Group. The amino acid sequence of CHYJ130330 E and M protein is highly conserved while ORF3 and N protein having 9 and 17 amino acid substitutions respectively in comparison to CV777 strain. The comparison of full length genome and the structural proteins revealed variations signifying that PEDV variant strains are still the main source of outbreaks in spite of continuous vaccination and also explain the variable trend of large scale outbreaks during this decade as compared to sporadic tendency of disease found before 2010. It is evident from this study that Chinese strains display significant level of mixing with the strains reported from other countries. The strain CHYJ130330 was also adapted successfully to Vero cell line and has shown high virulence in piglets. The information/findings will be helpful to develop a strategy for control of PEDV and have also shown that CHYJ130330 strain has strong virulence and is a more popular clinical strain in recent years, which has the potential to be developed into PEDV vaccine.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"7 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140594635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gut PathogensPub Date : 2024-04-05DOI: 10.1186/s13099-024-00614-y
François P. Douillard, Yağmur Derman, Ching Jian, Katri Korpela, Harri Saxén, Anne Salonen, Willem M. de Vos, Hannu Korkeala, Miia Lindström
{"title":"Case report: Aberrant fecal microbiota composition of an infant diagnosed with prolonged intestinal botulism","authors":"François P. Douillard, Yağmur Derman, Ching Jian, Katri Korpela, Harri Saxén, Anne Salonen, Willem M. de Vos, Hannu Korkeala, Miia Lindström","doi":"10.1186/s13099-024-00614-y","DOIUrl":"https://doi.org/10.1186/s13099-024-00614-y","url":null,"abstract":"Intestinal botulism is primarily reported in small babies as a condition known as infant botulism. The condition results from the ingestion of environmental or foodborne spores of botulinum neurotoxin (BoNT) producing Clostridia, usually Clostridium botulinum, and subsequent spore germination into active botulinum neurotoxinogenic cultures in the gut. It is generally considered that small babies are susceptible to C. botulinum colonization because of their immature gut microbiota. Yet, it is poorly understood which host factors contribute to the clinical outcome of intestinal botulism. We previously reported a case of infant botulism where the infant recovered clinically in six weeks but continued to secrete C. botulinum cells and/or BoNT in the feces for seven months. To further understand the microbial ecology behind this exceptionally long-lasting botulinum neurotoxinogenic colonization, we characterized the infant fecal microbiota using 16S rRNA gene amplicon sequencing over the course of disease and recovery. C. botulinum could be detected in the infant fecal samples at low levels through the acute phase of the disease and three months after recovery. Overall, we observed a temporal delay in the maturation of the infant fecal microbiota associated with a persistently high-level bifidobacterial population and a low level of Lachnospiraceae, Bacteroidaceae and Ruminococcaceae compared to healthy infants over time. This study brings novel insights into the infant fecal composition associated with intestinal botulism and provides a basis for a more systematic analysis of the gut microbiota of infants diagnosed with botulism. A better understanding of the gut microbial ecology associated with infant botulism may support the development of prophylactic strategies against this life-threatening disease in small babies.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"15 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140594409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gut PathogensPub Date : 2024-04-03DOI: 10.1186/s13099-024-00613-z
Karolina Kot, Adam Michaliszyn, Elżbieta Kalisińska, Małgorzata Lepczyńska
{"title":"The influence of plant extracts on viability of ST3 and ST7 subtypes of Blastocystis sp.","authors":"Karolina Kot, Adam Michaliszyn, Elżbieta Kalisińska, Małgorzata Lepczyńska","doi":"10.1186/s13099-024-00613-z","DOIUrl":"https://doi.org/10.1186/s13099-024-00613-z","url":null,"abstract":"Blastocystis sp. is one of the most frequently detected protozoa during stool specimen examination. In the last decade, the studies about the pathogenic potential of Blastocystis sp. have intensified. Additionally, treatment approaches against this parasite are still disputable. The study aimed to investigate the in vitro activity of the substances of natural origin against two subtypes (ST) of Blastocystis sp.—ST3 and ST7. Garlic and turmeric extracts exhibited the highest inhibitory effect in relation to the ST3 viability. While horseradish and turmeric were found to be the most effective extracts to the ST7 viability. The study showed that ginger, garlic, horseradish, and turmeric extracts have potent antimicrobial activity against Blastocystis ST3 and ST7, with the half-maximal inhibitory concentration (IC50) ranging from 3.8 to 4.8 µg/ml and from 3.3 to 72.0 µg/ml, respectively, and thus may be useful in the prevention and control of Blastocystis infections. Additionally, this research confirmed that Blastocystis ST7 is more resistant to the selected plant extracts treatment than Blastocystis ST3 which in consequence may bring some difficulties in its eradication.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"208 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140594406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gut PathogensPub Date : 2024-04-01DOI: 10.1186/s13099-024-00610-2
Nasser Mousa, Mohamed Salah, Sherif Elbaz, Alaa Elmetwalli, Amr Elhammady, Eman Abdelkader, Mostafa Abdelsalam, Niveen El-Wakeel, Marwa Mansour, Manal Hashem, Ola El-Emam, Wesam Elderiny, Mohammed Abdelaziz, Ayman Elgamal, Alaa Habib
{"title":"Neutrophil percentage-to-albumin ratio is a new diagnostic marker for spontaneous bacterial peritonitis: a prospective multicenter study.","authors":"Nasser Mousa, Mohamed Salah, Sherif Elbaz, Alaa Elmetwalli, Amr Elhammady, Eman Abdelkader, Mostafa Abdelsalam, Niveen El-Wakeel, Marwa Mansour, Manal Hashem, Ola El-Emam, Wesam Elderiny, Mohammed Abdelaziz, Ayman Elgamal, Alaa Habib","doi":"10.1186/s13099-024-00610-2","DOIUrl":"10.1186/s13099-024-00610-2","url":null,"abstract":"<p><strong>Background: </strong>The neutrophil percentage-to-albumin ratio (NPAR) is a novel measure of systemic inflammation and infection. Low albumin levels increase the risk of infection, while high neutrophil counts indicate the presence of infection. Spontaneous bacterial peritonitis (SBP) is a serious infection in cirrhotic ascites, and the potential of NPAR in diagnosing SBP is not yet established.</p><p><strong>Objective: </strong>The objective of this study is to determine the diagnostic value of NPAR in identifying SBP.</p><p><strong>Patients: </strong>This prospective multicenter study included 465 patients diagnosed with cirrhotic ascites and SBP according to international guidelines. Demographic, clinical, and laboratory data were collected. The sensitivity and specificity of NPAR values for diagnosing SBP were assessed using the receiver operating characteristic curve.</p><p><strong>Results: </strong>For SBP diagnosis in the total cohort, NPAR of > 17 had a sensitivity of 85.71%, specificity of 66.67%, and 95% CI (42.1-99.6). In culture-positive SBP, the NPAR at a cut-off > 5.2 had a sensitivity of 85.71%, specificity of 83.33%, and 95% CI (0.709 to 0.979), while in culture-negative SBP, the NPAR at a cut-off > 2.1 had a sensitivity of 92.86%, specificity of 33.33% and CI (0.367 to 0.764). The multivariate analysis revealed that albumin (OR = 2.78, [1.11;3.98], INR (OR = 0.198, [0.066;0.596], creatinine (OR = 0.292, [0.1; 0.81], CRP (OR = 3.18, [1.239;4.52] total leukocytic count (TLC) (OR = 1.97, [1.878; 2.07], platelets (OR = 2.09, [0.99; 2.31] and neutrophil (OR = 3.43, [1.04;3.89] were significantly associated with higher prediction rates for culture positive SBP.</p><p><strong>Conclusions: </strong>NPAR could be a new, affordable, noninvasive test for diagnosing SBP.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"16 1","pages":"18"},"PeriodicalIF":4.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10985869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"High prevalence of carbapenem resistance and clonal expansion of blaNDM gene in Klebsiella pneumoniae isolates in an Iranian referral pediatric hospital","authors":"Babak Pourakbari, Setareh Mamishi, Shiva Poormohammadi, Reihaneh Hosseinpour Sadeghi, Shima Mahmoudi","doi":"10.1186/s13099-024-00611-1","DOIUrl":"https://doi.org/10.1186/s13099-024-00611-1","url":null,"abstract":"The increasing global concern regarding antibiotic resistance necessitates in-depth studies to comprehend the phenotypic and genotypic characteristics of resistant bacterial strains. This study aimed to investigate the prevalence, antibiotic resistance profiles, and molecular characteristics of carbapenem-resistant Klebsiella pneumoniae (CRKP) isolates in an Iranian referral pediatrics hospital. Methods: In this study, we examined CRKP isolates collected from hospitalized pediatric patients across various wards. The isolates underwent antimicrobial susceptibility testing, the polymerase chain reaction (PCR) analysis for carbapenemase genes (blaNDM, blaVIM and blaIMP), and genetic relatedness assessment using pulsed-field gel electrophoresis (PFGE). Among 166 K. pneumoniae isolates, 54 (32.5%) exhibited resistance to carbapenems. Notably, all these resistant isolates were resistant to imipenem, with 35 (65%) displaying resistance to both imipenem and meropenem. Of the 54 CRKP isolates, 24 (44%) were metallo-β-lactamases (MBL)-producing. The prevalence of the blaNDM gene among CKCP and MBL-producing isolates was 20% (n = 11) and 44% (n = 24), respectively. The blaVIM and blaIMP genes were not detected in any of the isolates. Twenty-six CRKP isolates (48%) were recovered from ICUs. PFGE analysis of CRKP isolates revealed 20 clusters, with cluster S being the most prevalent, comprising 24% of the total (n = 13). Our study reveals a concerning prevalence of carbapenem resistance in K. pneumoniae isolates. Specifically, the detection of the blaNDM gene in 20% of CRKP isolates, with a significant proportion (82%) observed in isolated CRKP from the ICUs and emergency departments, underscores the potential clonal expansion of these resistant strains within these critical hospital wards.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"107 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140315239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gut PathogensPub Date : 2024-03-23DOI: 10.1186/s13099-024-00612-0
Ruta Inciuraite, Rolandas Gedgaudas, Rokas Lukosevicius, Deimante Tilinde, Rima Ramonaite, Alexander Link, Neringa Kasetiene, Mindaugas Malakauskas, Gediminas Kiudelis, Laimas Virginijus Jonaitis, Juozas Kupcinskas, Simonas Juzenas, Jurgita Skieceviciene
{"title":"Constituents of stable commensal microbiota imply diverse colonic epithelial cell reactivity in patients with ulcerative colitis.","authors":"Ruta Inciuraite, Rolandas Gedgaudas, Rokas Lukosevicius, Deimante Tilinde, Rima Ramonaite, Alexander Link, Neringa Kasetiene, Mindaugas Malakauskas, Gediminas Kiudelis, Laimas Virginijus Jonaitis, Juozas Kupcinskas, Simonas Juzenas, Jurgita Skieceviciene","doi":"10.1186/s13099-024-00612-0","DOIUrl":"10.1186/s13099-024-00612-0","url":null,"abstract":"<p><strong>Background: </strong>Despite extensive research on microbiome alterations in ulcerative colitis (UC), the role of the constituent stable microbiota remains unclear.</p><p><strong>Results: </strong>This study, employing 16S rRNA-gene sequencing, uncovers a persistent microbial imbalance in both active and quiescent UC patients compared to healthy controls. Using co-occurrence and differential abundance analysis, the study highlights microbial constituents, featuring Phocaeicola, Collinsella, Roseburia, Holdemanella, and Bacteroides, that are not affected during the course of UC. Co-cultivation experiments, utilizing commensal Escherichia coli and Phocaeicola vulgatus, were conducted with intestinal epithelial organoids derived from active UC patients and controls. These experiments reveal a tendency for a differential response in tight junction formation and maintenance in colonic epithelial cells, without inducing pathogen recognition and stress responses, offering further insights into the roles of these microorganisms in UC pathogenesis. These experiments also uncover high variation in patients' response to the same bacteria, which indicate the need for more comprehensive, stratified analyses with an expanded sample size.</p><p><strong>Conclusion: </strong>This study reveals that a substantial part of the gut microbiota remains stable throughout progression of UC. Functional experiments suggest that members of core microbiota - Escherichia coli and Phocaeicola vulgatus - potentially differentially regulate the expression of tight junction gene in the colonic epithelium of UC patients and healthy individuals.</p>","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"16 1","pages":"16"},"PeriodicalIF":4.2,"publicationDate":"2024-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10960424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gut PathogensPub Date : 2024-03-19DOI: 10.1186/s13099-024-00607-x
Sama Rezasoltani, Mehdi Azizmohammad Looha, Hamid Asadzadeh Aghdaei, Seyedesomayeh Jasemi, Leonardo Antonio Sechi, Maria Gazouli, Amir Sadeghi, Shirin Torkashvand, Reyhaneh Baniali, Hartmut Schlüter, Mohammad Reza Zali, Mohammad Mehdi Feizabadi
{"title":"Correction to: 16 S rRNA sequencing analysis of the oral and fecal microbiota in colorectal cancer positives versus colorectal cancer negatives in Iranian population","authors":"Sama Rezasoltani, Mehdi Azizmohammad Looha, Hamid Asadzadeh Aghdaei, Seyedesomayeh Jasemi, Leonardo Antonio Sechi, Maria Gazouli, Amir Sadeghi, Shirin Torkashvand, Reyhaneh Baniali, Hartmut Schlüter, Mohammad Reza Zali, Mohammad Mehdi Feizabadi","doi":"10.1186/s13099-024-00607-x","DOIUrl":"https://doi.org/10.1186/s13099-024-00607-x","url":null,"abstract":"<p><b>Correction to: Rezasoltani et al. Gut Pathogens 2024 Feb 20;16(1):9</b></p><p>https://doi.org/10.1186/s13099-024-00604-0</p><p>Following publication of the original article [1], it was pointed out that the affiliation details for all authors were incorrectly processed in the authorship line and published incorrectly.</p><p> The original article has been updated.</p><p> The affiliation information was mistakenly published as: Sama Rezasoltani<sup>1,7,8</sup>, Mehdi Azizmohammad Looha<sup>2,7</sup>, Hamid Asadzadeh Aghdaei<sup>2,7</sup>, Seyedesomayeh Jasemi<sup>3,7</sup>, Leonardo Antonio Sechi<sup>3,7,9*</sup>, Maria Gazouli<sup>4,7</sup>, Amir Sadeghi<sup>5,7</sup>, Shirin Torkashvand<sup>2,7</sup>, Reyhaneh Baniali<sup>2,7</sup>, Hartmut Schlüter<sup>1,7</sup>, Mohammad Reza Zali<sup>5,7</sup> and Mohammad Mehdi Feizabadi<sup>3,6,7 *</sup></p><p> The affiliation information should read as: Sama Rezasoltani<sup>1, 2</sup>, Mehdi Azizmohammad Looha<sup>3</sup>, Hamid Asadzadeh Aghdaei<sup>3</sup>, Seyedesomayeh Jasemi<sup>4</sup>, Leonardo Antonio Sechi<sup>4,5*</sup>, Maria Gazouli<sup>6</sup>, Amir Sadeghi<sup>7</sup>, Shirin Torkashvand<sup>3</sup>, Reyhaneh Baniali<sup>3</sup>, Hartmut Schlüter<sup>1</sup>, Mohammad Reza Zali<sup>7</sup>, Mohammad Mehdi Feizabadi <sup>8,9*</sup></p><h3>Authors and Affiliations</h3><ol><li><p>Section Mass Spectrometric Proteomics, Diagnostic Center, University Medical Center Hamburg-Eppendorf (UKE), 20246, Hamburg, Germany</p><p>Sama Rezasoltani & Hartmut Schlüter</p></li><li><p>Division of Oral Microbiology and Immunology, Department of Operative Dentistry, Periodontology and Preventive Dentistry, RWTH University Hospital, Pauwelsstrasse 30, 52057, Aachen, Germany</p><p>Sama Rezasoltani</p></li><li><p>Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, 19835-178, Iran</p><p>Mehdi Azizmohammad Looha, Hamid Asadzadeh Aghdaei, Shirin Torkashvand & Reyhaneh Baniali</p></li><li><p>Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43b, Sassari, 07100, Italy</p><p>Seyedesomayeh Jasemi & Leonardo Antonio Sechi</p></li><li><p>Struttura Complessa Microbiologia e Virologia, Azienda Ospedaliera Universitaria, Sassari, 07100, Italy</p><p>Leonardo Antonio Sechi</p></li><li><p>Department of Basic Medical Sciences, Laboratory of Biology, Medical School, National and Kapodistrian University of Athens, Athens, Greece</p><p>Maria Gazouli</p></li><li><p>Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, 19835-178, Iran</p><p>Amir Sadeghi & Mohammad Reza Zali</p></li><li><p>Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, 19835-178, Iran</p><p>Mohammad Mehdi Feizabadi</p></li><li><","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"41 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140165531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}