IF 2.3 3区生物学

Genome Pub Date : 2024-09-03 DOI: 10.1139/gen-2023-0094

Krystyn J Forbes, McIntyre A Barrera, Karsten Nielsen-Roine, Evan W Hersh, Jasmine K Janes, William L Harrower, Jamieson C Gorrell

{"title":"Stabilizing selection and mitochondrial heteroplasmy in the Canada lynx (Lynx canadensis).","authors":"Krystyn J Forbes, McIntyre A Barrera, Karsten Nielsen-Roine, Evan W Hersh, Jasmine K Janes, William L Harrower, Jamieson C Gorrell","doi":"10.1139/gen-2023-0094","DOIUrl":"10.1139/gen-2023-0094","url":null,"abstract":"Mitochondrial DNA is commonly used in population genetic studies to investigate spatial structure, intraspecific variation, and phylogenetic relationships. The control region is the most rapidly evolving and largest non-coding region, but its analysis can be complicated by heteroplasmic signals of genome duplication in many mammals, including felids. Here, we describe the presence of heteroplasmy in the control region of Canada lynx (Lynx canadensis) through intra-individual sequence variation. Our results demonstrate multiple haplotypes of varying length in each lynx, resulting from different copy numbers of the repetitive sequence RS-2 and suggest possible heteroplasmic single nucleotide polymorphisms in both repetitive sequences RS-2 and RS-3. Intra-individual variation was only observed in the repetitive sequences while inter-individual variation was detected in the flanking regions outside of the repetitive sequences, indicating that heteroplasmic mutations are restricted to these repeat regions. Although each lynx displayed multiple haplotypes of varying length, we found the most common variant contained three complete copies of the RS-2 repeat unit, suggesting copy number is regulated by stabilizing selection. While genome duplication offers potential for increased diversity, heteroplasmy may lead to a selective advantage or detriment in the face of mitochondrial function and disease, which could have significant implications for wildlife populations experiencing decline (e.g., bottlenecks) as a result of habitat modification or climate change.","PeriodicalId":12809,"journal":{"name":"Genome","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome mapping of retrotransposon AviRTE in a neotropical bird species: Trogon surrucura (Trogoniformes; Trogonidae). 新热带鸟类逆转录转座子 AviRTE 的染色体图谱：Trogon surrucura (Trogoniformes; Trogonidae)。

IF 2.3 3区生物学

Genome Pub Date : 2024-09-01 Epub Date: 2024-05-09 DOI: 10.1139/gen-2023-0075

Nairo Farias de Farias, Ricardo José Gunski, Analía Del Valle Garnero, Andrés Delgado Cañedo, Edivaldo Herculano Correa de Oliveira, Fábio Augusto Oliveira Silva, Fabiano Pimentel Torres

{"title":"Chromosome mapping of retrotransposon AviRTE in a neotropical bird species: Trogon surrucura (Trogoniformes; Trogonidae).","authors":"Nairo Farias de Farias, Ricardo José Gunski, Analía Del Valle Garnero, Andrés Delgado Cañedo, Edivaldo Herculano Correa de Oliveira, Fábio Augusto Oliveira Silva, Fabiano Pimentel Torres","doi":"10.1139/gen-2023-0075","DOIUrl":"10.1139/gen-2023-0075","url":null,"abstract":"Avian genomes are characterized as being more compact than other amniotes, with less diversity and density of transposable elements (TEs). In addition, birds usually show bimodal karyotypes, exhibiting a great variation in diploid numbers. Some species present unusually large sex chromosomes, possibly due to the accumulation of repetitive sequences. Avian retrotransposon-like element (AviRTE) is a long interspersed nuclear element (LINE) recently discovered in the genomes of birds and nematodes, and it is still poorly characterized in terms of chromosomal mapping and phylogenetic relationships. In this study, we mapped AviRTE isolated from the Trogon surrucura genome into the T. surrucura (TSU) karyotype. Furthermore, we analyzed the phylogenetic relationships of this LINE in birds and other vertebrates. Our results showed that the distribution pattern of AviRTE is not restricted to heterochromatic regions, with accumulation on the W chromosome of TSU, yet another species with an atypical sex chromosome and TE hybridization. The phylogenetic analysis of AviRTE sequences in birds agreed with the proposed phylogeny of species in most clades, and allowed the detection of this sequence in other species, expanding the distribution of the element.","PeriodicalId":12809,"journal":{"name":"Genome","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosomal and genomic analysis suggests single origin and high molecular differentiation of the B chromosome of Abracris flavolineata. 染色体和基因组分析表明，Abracris flavolineata 的 B 染色体起源单一，分子分化程度较高。

IF 2.3 3区生物学

Genome Pub Date : 2024-09-01 Epub Date: 2024-05-09 DOI: 10.1139/gen-2023-0122

Diogo Milani, Ana Elisa Gasparotto, Vilma Loreto, Dardo A Martí, Diogo C Cabral-de-Mello

{"title":"Chromosomal and genomic analysis suggests single origin and high molecular differentiation of the B chromosome of Abracris flavolineata.","authors":"Diogo Milani, Ana Elisa Gasparotto, Vilma Loreto, Dardo A Martí, Diogo C Cabral-de-Mello","doi":"10.1139/gen-2023-0122","DOIUrl":"10.1139/gen-2023-0122","url":null,"abstract":"Supernumerary chromosomes (B chromosomes) have been an intriguing subject of study. Our understanding of the molecular differentiation of B chromosomes from an interpopulation perspective remains limited, with most analyses involving chromosome banding and mapping of a few sequences. To gain insights into the molecular composition, origin, and evolution of B chromosomes, we conducted cytogenetic and next-generation sequencing analysis of the repeatome in the grasshopper Abracris flavolineata across various populations. Our results unveiled the presence of B chromosomes in two newly investigated populations and described new satellite DNA sequences. While we observed some degree of genetic connection among A. flavolineata populations, our comparative analysis of genomes with and without B chromosomes provided evidence of two new B chromosome variants. These variants exhibited distinct compositions of various repeat classes, including transposable elements and satellite DNAs. Based on shared repeats, their chromosomal location, and the C-positive heterochromatin content on the B chromosome, these variants likely share a common origin but have undergone distinct molecular differentiation processes, resulting in varying degrees of heterochromatinization. Our data serve as a detailed example of the dynamic and differentiated nature of B chromosome molecular content at the interpopulation level, even when they share a common origin.","PeriodicalId":12809,"journal":{"name":"Genome","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of defensin genes across house fly (Musca domestica) life history gives insight into immune system subfunctionalization. 家蝇（Musca domestica）生命史中防御素基因的表达深入揭示了免疫系统的亚功能化。

IF 2.3 3区生物学

Genome Pub Date : 2024-09-01 Epub Date: 2024-05-09 DOI: 10.1139/gen-2024-0016

Danial Asgari, Tanya Purvis, Victoria Pickens, Christopher Saski, Richard P Meisel, Dana Nayduch

{"title":"Expression of defensin genes across house fly (Musca domestica) life history gives insight into immune system subfunctionalization.","authors":"Danial Asgari, Tanya Purvis, Victoria Pickens, Christopher Saski, Richard P Meisel, Dana Nayduch","doi":"10.1139/gen-2024-0016","DOIUrl":"10.1139/gen-2024-0016","url":null,"abstract":"Animals encounter diverse microbial communities throughout their lifetime, which exert varying selection pressures. Antimicrobial peptides (AMPs), which lyse or inhibit microbial growth, are a first line of defense against some of these microbes. Here we examine how developmental variation in microbial exposure has affected the evolution of expression and amino acid sequences of Defensins (an ancient class of AMPs) in the house fly (Musca domestica). The house fly is a well-suited model for this work because it trophically associates with varying microbial communities throughout its life history and its genome contains expanded families of AMPs, including Defensins. We identified two subsets of house fly Defensins: one expressed in larvae or pupae, and the other expressed in adults. The amino acid sequences of these two Defensin subsets form distinct monophyletic clades, and they are located in separate gene clusters in the genome. The adult-expressed Defensins evolve faster than larval/pupal Defensins, consistent with different selection pressures across developmental stages. Our results therefore suggest that varied microbial communities encountered across life history can shape the evolutionary trajectories of immune genes.","PeriodicalId":12809,"journal":{"name":"Genome","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140897921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fragments derived from non-coding RNAs: how complex is genome regulation? 非编码 RNA 片段：基因组调控有多复杂？

IF 2.3 3区生物学

Genome Pub Date : 2024-09-01 Epub Date: 2024-04-29 DOI: 10.1139/gen-2023-0136

Miguel Ángel Velázquez-Flores, Ruth Ruiz Esparza-Garrido

引用次数: 0

Understanding microchromosomal organization and evolution in four representative woodpeckers (Picidae, Piciformes) through BAC-FISH analysis. 通过 BAC-FISH 分析了解四种代表性啄木鸟（啄木鸟科、啄木鸟属）的微染色体组织和进化。

IF 2.3 3区生物学

Genome Pub Date : 2024-07-01 Epub Date: 2024-05-14 DOI: 10.1139/gen-2023-0096

Suziane Alves Barcellos, Rafael Kretschmer, Marcelo Santos de Souza, Victoria Tura, Luciano Cesar Pozzobon, Thales Renato Ochotorena de Freitas, Darren K Griffin, Rebecca O'Connor, Ricardo José Gunski, Analía Del Valle Garnero

{"title":"Understanding microchromosomal organization and evolution in four representative woodpeckers (Picidae, Piciformes) through BAC-FISH analysis.","authors":"Suziane Alves Barcellos, Rafael Kretschmer, Marcelo Santos de Souza, Victoria Tura, Luciano Cesar Pozzobon, Thales Renato Ochotorena de Freitas, Darren K Griffin, Rebecca O'Connor, Ricardo José Gunski, Analía Del Valle Garnero","doi":"10.1139/gen-2023-0096","DOIUrl":"10.1139/gen-2023-0096","url":null,"abstract":"The genome organization of woodpeckers has several distinctive features e.g., an uncommon accumulation of repetitive sequences, enlarged Z chromosomes, and atypical diploid numbers. Despite the large diversity of species, there is a paucity of detailed cytogenomic studies for this group and we thus aimed to rectify this. Genome organization patterns and hence evolutionary change in the microchromosome formation of four species (Colaptes campestris, Veniliornis spilogaster, Melanerpes candidus, and Picumnus nebulosus) was established through fluorescence in situ hybridization using bacterial artificial chromosomes originally derived from Gallus gallus and Taeniopygia guttata. Findings suggest that P. nebulosus (2n = 110), which was described for the first time, had the most basal karyotype among species of Picidae studied here, and probably arose as a result of fissions of avian ancestral macrochromosomes. We defined a new chromosomal number for V. spilogaster (2n = 88) and demonstrated microchromosomal rearrangements involving C. campestris plus a single, unique hitherto undescribed rearrangement in V. spilogaster. This comprised an inversion after a fusion involving the ancestral microchromosome 12 (homologous to chicken microchromosome 12). We also determined that the low diploid number of M. candidus is related to microchromosome fusions. Woodpeckers thus exhibit significantly rearranged karyotypes compared to the putative ancestral karyotype.","PeriodicalId":12809,"journal":{"name":"Genome","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic prediction in Brassica napus: evaluating the benefit of imputed whole-genome sequencing data. 甘蓝型油菜的基因组预测：评估估算全基因组测序数据的益处。

IF 2.3 3区生物学

Genome Pub Date : 2024-07-01 Epub Date: 2024-05-06 DOI: 10.1139/gen-2023-0126

Sven E Weber, Lennard Roscher-Ehrig, Tobias Kox, Amine Abbadi, Andreas Stahl, Rod J Snowdon

{"title":"Genomic prediction in Brassica napus: evaluating the benefit of imputed whole-genome sequencing data.","authors":"Sven E Weber, Lennard Roscher-Ehrig, Tobias Kox, Amine Abbadi, Andreas Stahl, Rod J Snowdon","doi":"10.1139/gen-2023-0126","DOIUrl":"10.1139/gen-2023-0126","url":null,"abstract":"Advances in sequencing technology allow whole plant genomes to be sequenced with high quality. Combining genotypic and phenotypic data in genomic prediction helps breeders to select crossing partners in partially phenotyped populations. In plant breeding programs, the cost of sequencing entire breeding populations still exceeds available genotyping budgets. Hence, the method for genotyping is still mainly single nucleotide polymorphism (SNP) arrays; however, arrays are unable to assess the entire genome- and population-wide diversity. A compromise involves genotyping the entire population using an SNP array and a subset of the population with whole-genome sequencing. Both datasets can then be used to impute markers from whole-genome sequencing onto the entire population. Here, we evaluate whether imputation of whole-genome sequencing data enhances genomic predictions, using data from a nested association mapping population of rapeseed (Brassica napus). Employing two cross-validation schemes that mimic scenarios for the prediction of close and distant relatives, we show that imputed marker data do not significantly improve prediction accuracy, likely due to redundancy in relationship estimates and imputation errors. In simulation studies, only small improvements were observed, further corroborating the findings. We conclude that SNP arrays are already equipped with the information that is added by imputation through relationship and linkage disequilibrium.","PeriodicalId":12809,"journal":{"name":"Genome","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the chromosomal evolution in cuckoos (Aves, Cuculiformes): a journey through unusual rearrangements. 了解杜鹃（鸟类，鹃形目）的染色体进化：非同寻常的重排之旅。

IF 3.1 3区生物学

Genome Pub Date : 2024-06-01 Epub Date: 2024-02-12 DOI: 10.1139/gen-2023-0101

Rafael Kretschmer, Marcelo Santos de Souza, Ricardo José Gunski, Analía Del Valle Garnero, Thales Renato Ochotorena de Freitas, Edison Zefa, Gustavo Akira Toma, Marcelo de Bello Cioffi, Edivaldo Herculano Corrêa de Oliveira, Rebecca E O'Connor, Darren K Griffin

{"title":"Understanding the chromosomal evolution in cuckoos (Aves, Cuculiformes): a journey through unusual rearrangements.","authors":"Rafael Kretschmer, Marcelo Santos de Souza, Ricardo José Gunski, Analía Del Valle Garnero, Thales Renato Ochotorena de Freitas, Edison Zefa, Gustavo Akira Toma, Marcelo de Bello Cioffi, Edivaldo Herculano Corrêa de Oliveira, Rebecca E O'Connor, Darren K Griffin","doi":"10.1139/gen-2023-0101","DOIUrl":"10.1139/gen-2023-0101","url":null,"abstract":"The Cuculiformes are a family of over 150 species that live in a range of habitats, such as forests, savannas, and deserts. Here, bacterial artificial chromosome (BAC) probes (75 from chicken and 14 from zebra finch macrochromosomes 1-10 +ZW and for microchromosomes 11-28 (except 16)) were used to investigate chromosome homologies between chicken and the squirrel cuckoo (Piaya cayana). In addition, repetitive DNA probes were applied to characterize the chromosome organization and to explore the role of these sequences in the karyotype evolution of P. cayana. We also applied BAC probes for chicken chromosome 17 and Z to the guira cuckoo (Guira guira) to test whether this species has an unusual Robertsonian translocation between a microchromosome and the Z chromosome, recently described in the smooth-billed ani (Crotophaga ani). Our results revealed extensive chromosome reorganization with inter- and intrachromosomal rearrangements in P. cayana, including a conspicuous chromosome size and heterochromatin polymorphism on chromosome pair 20. Furthermore, we confirmed that the Z-autosome Robertsonian translocation found in C. ani is also found in G. guira, not P. cayana. These findings suggest that this translocation occurred prior to the divergence between C. ani and G. guira, but after the divergence with P. cayana.","PeriodicalId":12809,"journal":{"name":"Genome","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meta-analyses of the global multilocus genotypes of the human pathogen Campylobacter jejuni. 人类病原体空肠弯曲杆菌全球多焦点基因型的元分析。

IF 2.3 3区生物学

Genome Pub Date : 2024-06-01 Epub Date: 2024-03-01 DOI: 10.1139/gen-2023-0041

Monir Poorrashidi, Megan Hitchcock, Jianping Xu

{"title":"Meta-analyses of the global multilocus genotypes of the human pathogen Campylobacter jejuni.","authors":"Monir Poorrashidi, Megan Hitchcock, Jianping Xu","doi":"10.1139/gen-2023-0041","DOIUrl":"10.1139/gen-2023-0041","url":null,"abstract":"Campylobacter infections are a leading cause of bacterial diarrheal illness worldwide, with increasing reports of outbreaks in both developing and developed countries. Most studies investigating strain genotypes and epidemiology of Campylobacter jejuni examined on a local scale. Using the archived multilocus sequence typing data at seven loci, and associated strain metadata from the PubMLST database, here we investigated the spatial and temporal genetic structure of the global population of C. jejuni. Our analyses revealed evidence for clonal dispersals of multiple sequence types (STs) among countries and continents. However, despite the observed clonal dispersal and that most genetic variations were found within individual geographic subpopulations, both the non-clone-corrected and clone-corrected samples showed evidence of significant genetic differentiation among national and continental subpopulations, with non-clone-corrected samples showing greater differentiation than clone-corrected samples. Phylogenetic incompatibility analyses provided evidence for recombination within each continental subpopulation. However, linkage disequilibrium analyses rejected the hypothesis of random recombination across the samples. Temporally, multiple STs were found to persist across four decades and the five globally most common STs showed relatively stable frequencies over the last two decades. We discussed the implications of our results to food security, disease transmission, and public health management.","PeriodicalId":12809,"journal":{"name":"Genome","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140012466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and application of whole-chromosome painting of chromosomes 7A and 8A of Arachis duranensis based on chromosome-specific single-copy oligonucleotides. 基于染色体特异性单拷贝寡核苷酸的杜兰花7A和8A染色体全染色体涂片的开发与应用

IF 3.1 3区生物学

Genome Pub Date : 2024-06-01 Epub Date: 2024-02-23 DOI: 10.1139/gen-2023-0116

Chenyu Li, Liuyang Fu, Qian Wang, Hua Liu, Guoquan Chen, Feiyan Qi, Maoning Zhang, Yaoguang Jia, Xiaona Li, Bingyan Huang, Wenzhao Dong, Pei Du, Xinyou Zhang

{"title":"Development and application of whole-chromosome painting of chromosomes 7A and 8A of Arachis duranensis based on chromosome-specific single-copy oligonucleotides.","authors":"Chenyu Li, Liuyang Fu, Qian Wang, Hua Liu, Guoquan Chen, Feiyan Qi, Maoning Zhang, Yaoguang Jia, Xiaona Li, Bingyan Huang, Wenzhao Dong, Pei Du, Xinyou Zhang","doi":"10.1139/gen-2023-0116","DOIUrl":"10.1139/gen-2023-0116","url":null,"abstract":"For peanut, the lack of stable cytological markers is a barrier to tracking specific chromosomes, elucidating the genetic relationships between genomes and identifying chromosomal variations. Chromosome mapping using single-copy oligonucleotide (oligo) probe libraries has unique advantages for identifying homologous chromosomes and chromosomal rearrangements. In this study, we developed two whole-chromosome single-copy oligo probe libraries, LS-7A and LS-8A, based on the reference genome sequences of chromosomes 7A and 8A of Arachis duranensis. Fluorescence in situ hybridization (FISH) analysis confirmed that the libraries could specifically paint chromosomes 7 and 8. In addition, sequential FISH and electronic localization of LS-7A and LS-8A in A. duranensis (AA) and A. ipaensis (BB) showed that chromosomes 7A and 8A contained translocations and inversions relative to chromosomes 7B and 8B. Analysis of the chromosomes of wild Arachis species using LS-8A confirmed that this library could accurately and effectively identify A genome species. Finally, LS-7A and LS-8A were used to paint the chromosomes of interspecific hybrids and their progenies, which verified the authenticity of the interspecific hybrids and identified a disomic addition line. This study provides a model for developing specific oligo probes to identify the structural variations of other chromosomes in Arachis and demonstrates the practical utility of LS-7A and LS-8A.","PeriodicalId":12809,"journal":{"name":"Genome","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139939909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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