IF 3.1 3区生物学

Genome Pub Date : 2022-05-16 DOI: 10.1139/gen-2021-0085

Ziting Huo, Wenbo Xu, Huijun Guo, Peng-Feil Yang, Qianwen Zhang, Xu Lu, Long Wang

{"title":"The complete chloroplast genome of Persicaria perfoliata and comparative analysis with Four Medicinal Plants of Polygonaceae.","authors":"Ziting Huo, Wenbo Xu, Huijun Guo, Peng-Feil Yang, Qianwen Zhang, Xu Lu, Long Wang","doi":"10.1139/gen-2021-0085","DOIUrl":"https://doi.org/10.1139/gen-2021-0085","url":null,"abstract":"Polygonaceae is a large family of medicinal herbs that includes many species used as traditional Chinese medicine, such as Persicaria perfoliate. Here, we sequenced the complete cp genome of P. perfoliata using Illumina sequencing technology with the purpose to provide a method to facilitate accurate identification. After being annotated, the cp genome of P. perfoliata was compared with Fagopyrum tataricum, Persicaria chinensis, Fagopyrum dibotrys and Fallopia multiflora. The complete cp genome of P. perfoliata is 160,730 bp in length, containing a small single copy (SSC) region of 12,927 bp, a large single copy (LSC) region of 85,433 bp and a pair of inverted repeats (IR) regions of 62,370 bp. A total of 131 genes were annotated, including eight rRNA genes, 34 tRNA genes and 84 protein-coding genes. Forty-two simple sequence repeats and fifty-five repeat sequences were identified. Mutational hot spots analyses indicated that five genes (matK, ndhF, ccsA, cemA, rpl20) could be selected as candidates for molecular markers. Moreover, phylogenetic analysis showed that all the Polygonaceae species formed a monophyletic clade, and P. perfoliata showed the closest relationship with P. chinense. The study provides valuable molecular information to accurately identify P. perfoliata and assist in its development and application.","PeriodicalId":12809,"journal":{"name":"Genome","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47024058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete chloroplast genome of Gentianopsis barbata and comparative analysis with related species from Gentianaceae. 龙胆草叶绿体全基因组及其与龙胆草科近缘种的比较分析。

IF 3.1 3区生物学

Genome Pub Date : 2022-05-07 DOI: 10.1139/gen-2021-0080

Zhan Feng, Yan Zheng, Yuan Jiang, Yu-jing Miao, G. Luo, Linfang Huang

{"title":"Complete chloroplast genome of Gentianopsis barbata and comparative analysis with related species from Gentianaceae.","authors":"Zhan Feng, Yan Zheng, Yuan Jiang, Yu-jing Miao, G. Luo, Linfang Huang","doi":"10.1139/gen-2021-0080","DOIUrl":"https://doi.org/10.1139/gen-2021-0080","url":null,"abstract":"Gentianopsis barbata is an essential medicinal plant in China with high ornamental and medicinal values. Unfortunately, the study of the chloroplast genome of this plant still has a gap. This study sequenced and characterized the complete chloroplast genome of G. barbata. The complete chloroplast genome of G. barbata is a typical circular structure with 151,123 bp. It consists of a large single-copy region (82,690 bp) and a small single-copy region (17,887 bp) separated by a pair of inverted repeats (25,273 bp), which covers 78 protein-coding genes, 30 tRNAs, and 4 rRNAs. Repeat analysis showed the highest frequency of palindrome. Thirty-seven simple sequence repeats were identified, most of which were single nucleotides. The bayesian inference tree, maximum likelihood tree, and neighbor joining tree suggested that G. barbata is grouped with Gentianopsis grandis and Gentianopsis paludosa. The divergence time analysis showed that G. barbata diverged at 1.243 Mya. Comparative chloroplast analysis can reveal interspecific diversity, and regions with high variation can be used to develop molecular markers applicable to various research areas. Our results provide new insight into plastome evolution and valuable resource for further studies on G. barbata.","PeriodicalId":12809,"journal":{"name":"Genome","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46179387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Characterization and Expression of the Pirin Gene Family in Triticum aestivum. Pirin基因家族在小麦中的表达与鉴定。

IF 3.1 3区生物学

Genome Pub Date : 2022-05-03 DOI: 10.1139/gen-2021-0094

Sabrina C Brunetti, Michelle K. M. Arseneault, Patrick J. Gulick

{"title":"Characterization and Expression of the Pirin Gene Family in Triticum aestivum.","authors":"Sabrina C Brunetti, Michelle K. M. Arseneault, Patrick J. Gulick","doi":"10.1139/gen-2021-0094","DOIUrl":"https://doi.org/10.1139/gen-2021-0094","url":null,"abstract":"Pirins are nuclear bicupin proteins, encoded by genes that are one of several gene families that comprise the Cupin superfamily in plants. Pirin genes have been implicated in stress response pathways studied in Arabidopsis and At-Pirin1 has been shown to interact with the heterotrimeric G-protein alpha subunit (GPA1). The aim of this study was to identify the members of the Pirin gene family in Triticum aestivum, to correct their annotations in the whole genome and gain an insight into their tissue-specific expression as well as their response to abiotic and biotic stresses. The Pirin gene family in T. aestivum is comprised of 18 genes that represent six paralogous gene copies, each having an A, B and D homeolog. Expression analysis of the Pirin genes in T. aestivum Illumina RNA-seq libraries, which included sampling from differing tissue types as well as abiotic and biotic stresses, indicates that the members of the Pirin gene family have specialized expression and play a role in stress responses. Pirin gene families are also identified in other monocots including Aegilops tauschii, Hordeum vulgare, Brachypodium distachyon, Oryza sativa, Zea mays, Sorghum bicolor and the dicot Arabidopsis thaliana.","PeriodicalId":12809,"journal":{"name":"Genome","volume":" ","pages":""},"PeriodicalIF":3.1,"publicationDate":"2022-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42898246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Molecular analysis of nematode-responsive defence genes CRF1, WRKY45, and PR7 in Solanum lycopersicum tissues during the infection of plant-parasitic nematode species of the genus Meloidogyne. 茄属植物寄生线虫感染时组织中线虫应答防御基因CRF1、WRKY45和PR7的分子分析

IF 3.1 3区生物学

Genome Pub Date : 2022-05-01 Epub Date: 2022-02-03 DOI: 10.1139/gen-2021-0083

Refik Bozbuga

{"title":"Molecular analysis of nematode-responsive defence genes CRF1, WRKY45, and PR7 in Solanum lycopersicum tissues during the infection of plant-parasitic nematode species of the genus Meloidogyne.","authors":"Refik Bozbuga","doi":"10.1139/gen-2021-0083","DOIUrl":"https://doi.org/10.1139/gen-2021-0083","url":null,"abstract":"Several pathogens, including nematodes, have severe effects on plant development and growth, and immense populations of parasitic nematodes may cause plant death and crop loss. Obligate plant-parasitic nematodes and root-knot nematodes belonging to the genus Meloidogyne are significant parasites in crops. During nematode infection, damage-associated molecular patterns play a role in the activation of plant defence responses to pathogens. Several genes are involved in Meloidogyne parasitism. However, the expression of nematode-responsive genes CRF1, WRKY45, and PR7 during infection with different parasitic nematode species is not well understood. Therefore, this study aimed to reveal plant responses to differential gene expression of nematode-responsive genes in tomato plants, and their relationship to nematode reproduction and comparative phylogeny. Molecular methods for gene expression, greenhouse work for nematode reproduction, and phylogenetic analysis were used to determine nematode-plant interactions. The results revealed that differential gene expression of CRF1, WRKY45, and PR7 depended on the nematode species. The relative CRF1 gene expression reached its highest level at 3 dpi, following nematode infection. In conclusion, plant defense responses disturbed the expression of nematode-responsive genes, and the differential expression of nematode-responsive genes was affected by nematode species and nematode parasitism.","PeriodicalId":12809,"journal":{"name":"Genome","volume":"65 5","pages":"265-275"},"PeriodicalIF":3.1,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39745960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

An endogenous retrovirus presumed to have been endogenized or relocated recently in a marsupial, the red-necked wallaby. 一种内源性逆转录病毒，推测最近在一种有袋动物，红颈小袋鼠体内被内源性或重新安置。

IF 3.1 3区生物学

Genome Pub Date : 2022-05-01 Epub Date: 2022-01-14 DOI: 10.1139/gen-2021-0047

Sakura Hayashi, Konami Shimizu, Yusuke Honda, Yukako Katsura, Akihiko Koga

{"title":"An endogenous retrovirus presumed to have been endogenized or relocated recently in a marsupial, the red-necked wallaby.","authors":"Sakura Hayashi, Konami Shimizu, Yusuke Honda, Yukako Katsura, Akihiko Koga","doi":"10.1139/gen-2021-0047","DOIUrl":"https://doi.org/10.1139/gen-2021-0047","url":null,"abstract":"An albino infant wallaby was born to a mother with wild-type body color. PCR and sequencing analyses of TYR (encoding tyrosinase, which is essential for melanin biosynthesis) of this albino wallaby revealed a 7.1-kb-long DNA fragment inserted in the first exon. Since the fragment carried long terminal repeats, we assumed it to be a copy of an endogenous retrovirus, which we named walb. We cloned other walb copies residing in the genomes of this species and of another wallaby species. The copies exhibited length variation, and the longest copy (>8.0 kb) contained open reading frames whose deduced amino acid sequences were well aligned with those of gag, pol, and env of retroviruses. It is unknown through which of the following likely processes the walb copy was inserted into TYR: endogenization (infection of a germline cell by an exogenous virus), reinfection (infection by a virus produced from a previously endogenized provirus), or retrotransposition (intracellular relocation of a provirus). In any case, the insertion into TYR is considered to have been a recent event on an evolutionary timescale because albino mutant alleles generally do not persist for long because of their deleterious effects in wild circumstances.","PeriodicalId":12809,"journal":{"name":"Genome","volume":"65 5","pages":"277-286"},"PeriodicalIF":3.1,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39820887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Genomic inversions in Escherichia coli alter gene expression and are associated with nucleoid protein binding sites. 大肠杆菌的基因组倒置改变了基因表达，并与类核蛋白结合位点相关。

IF 3.1 3区生物学

Genome Pub Date : 2022-05-01 Epub Date: 2022-01-24 DOI: 10.1139/gen-2021-0102

Daniella F Lato, Qing Zeng, G Brian Golding

{"title":"Genomic inversions in Escherichia coli alter gene expression and are associated with nucleoid protein binding sites.","authors":"Daniella F Lato, Qing Zeng, G Brian Golding","doi":"10.1139/gen-2021-0102","DOIUrl":"https://doi.org/10.1139/gen-2021-0102","url":null,"abstract":"Genomic reorganization, such as rearrangements and inversions, influences how genetic information is organized within the bacterial genomes. Inversions, in particular, facilitate genome evolution through gene gain and loss, and can alter gene expression. Previous studies have investigated the impact inversions have on gene expression induced inversions targeting specific genes or examine inversions between distantly related species. This fails to encompass a genome-wide perspective of naturally occurring inversions and their post-adaptation impact on gene expression. Here, we used bioinformatic techniques and multiple RNA-seq datasets to investigate the short- and long-range impact inversions have on genomic gene expression within Escherichia coli. We observed differences in gene expression between homologous inverted and non-inverted genes even after long-term exposure to adaptive selection. In 4% of inversions representing 33 genes, differential gene expression between inverted and non-inverted homologs was detected, with greater than two-thirds (71%) of differentially expressed inverted genes having 9.4-85.6-fold higher gene expression. The identified inversions had more overlap than expected with nucleoid-associated protein binding sites, which assist in the regulation of genomic gene expression. Some inversions can drastically impact gene expression, even between different strains of E. coli, and could provide a mechanism for the diversification of genetic content through controlled expression changes.","PeriodicalId":12809,"journal":{"name":"Genome","volume":"65 5","pages":"287-299"},"PeriodicalIF":3.1,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39963031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Whole-genome comparative analysis reveals genetic mechanisms of disease resistance and heat tolerance of tropical Bos indicus cattle breeds. 全基因组比较分析揭示了热带籼稻牛品种抗病性和耐热性的遗传机制。

IF 3.1 3区生物学

Genome Pub Date : 2022-04-01 Epub Date: 2021-12-16 DOI: 10.1139/gen-2021-0030

Periyasamy Vijayakumar, Arunasalam Singaravadivelan, Anamika Mishra, Krishnan Jagadeesan, Sanniyasi Bakyaraj, Ramalingam Suresh, Thiagarajan Sivakumar

{"title":"Whole-genome comparative analysis reveals genetic mechanisms of disease resistance and heat tolerance of tropical Bos indicus cattle breeds.","authors":"Periyasamy Vijayakumar, Arunasalam Singaravadivelan, Anamika Mishra, Krishnan Jagadeesan, Sanniyasi Bakyaraj, Ramalingam Suresh, Thiagarajan Sivakumar","doi":"10.1139/gen-2021-0030","DOIUrl":"https://doi.org/10.1139/gen-2021-0030","url":null,"abstract":"Bos indicus cattle breeds have been naturally selected for thousands of years for disease resistance and thermo-tolerance. However, the genetic mechanisms underlying these specific inherited characteristics must be elucidated. Hence, in this study, a whole-genome comparative analysis of the Bos indicus cattle breeds Kangayam, Tharparkar, Sahiwal, Red Sindhi, and Hariana of the Indian subcontinent was conducted. Genetic variant identification analysis revealed 155 851 012 SNPs and 10 062 805 InDels in the mapped reads across all Bos indicus cattle breeds. The functional annotation of 17 252 genes that comprised both SNPs and InDels, with high functional impact on proteins, was carried out. The functional annotation results revealed the pathways involved in the innate immune response, including toll-like receptors, retinoic acid-inducible gene I-like receptors, NOD-like receptors, Jak-STAT signaling pathways, and non-synonymous variants in the candidate immune genes. We also identified several pathways involved in the heat shock response, hair and skin properties, oxidative stress response, osmotic stress response, thermal sweating, feed intake, metabolism, and non-synonymous variants in the candidate thermo-tolerant genes. These pathways and genes directly or indirectly contribute to the disease resistance and thermo-tolerance adaptations of Bos indicus cattle breeds.","PeriodicalId":12809,"journal":{"name":"Genome","volume":"65 4","pages":"241-254"},"PeriodicalIF":3.1,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39730615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Frequent numerical and structural chromosome changes in early generations of synthetic hexaploid wheat. 合成六倍体小麦早期世代染色体数量和结构的频繁变化。

IF 3.1 3区生物学

Genome Pub Date : 2022-04-01 Epub Date: 2021-12-16 DOI: 10.1139/gen-2021-0074

Siyu Zhang, Pei Du, Xueying Lu, Jiaxin Fang, Jiaqi Wang, Xuejun Chen, Jianyong Chen, Hao Wu, Yang Yang, Hisashi Tsujimoto, Chenggen Chu, Zengjun Qi

{"title":"Frequent numerical and structural chromosome changes in early generations of synthetic hexaploid wheat.","authors":"Siyu Zhang, Pei Du, Xueying Lu, Jiaxin Fang, Jiaqi Wang, Xuejun Chen, Jianyong Chen, Hao Wu, Yang Yang, Hisashi Tsujimoto, Chenggen Chu, Zengjun Qi","doi":"10.1139/gen-2021-0074","DOIUrl":"https://doi.org/10.1139/gen-2021-0074","url":null,"abstract":"Modern hexaploid wheat (Triticum aestivum L.; AABBDD) evolved from a hybrid of tetraploid wheat (closely related to Triticum turgidum L. ssp. durum (Desf.) Husn., AABB) and goatgrass (Aegilops tauschii Coss., DD). Variations in chromosome structure and ploidy played important roles in wheat evolution. How these variations occurred and their role in expanding the genetic diversity in modern wheat is mostly unknown. Synthetic hexaploid wheat (SHW) can be used to investigate chromosome variation that occurs during the early generations of existence. SHW lines derived by crossing durum wheat 'Langdon' with twelve Ae. tauschii accessions were analyzed using oligonucelotide probe multiplex fluorescence in situ hybridization (FISH) to metaphase chromosomes and SNP markers. Cluster analysis based on SNP markers categorized them into three groups. Among 702 plants from the S8 and S9 generations, 415 (59.12%) carried chromosome variations involving all 21 chromosomes but with different frequencies for each chromosome and sub-genome. Total chromosome variation frequencies varied between lines, but there was no significant difference among the three groups. The non-random chromosome variations in SHW lines detected in this research may be an indication that similar variations occurred in the early stages of wheat polyploidization and played important roles in wheat evolution.","PeriodicalId":12809,"journal":{"name":"Genome","volume":"65 4","pages":"205-217"},"PeriodicalIF":3.1,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39730875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lack of variation in nuclear DNA content in avian muscle. 禽肌肉核DNA含量缺乏变异。

IF 3.1 3区生物学

Genome Pub Date : 2022-04-01 Epub Date: 2021-12-02 DOI: 10.1139/gen-2021-0052

Ana Gabriela Jimenez, Emily Gray Lencyk

{"title":"Lack of variation in nuclear DNA content in avian muscle.","authors":"Ana Gabriela Jimenez, Emily Gray Lencyk","doi":"10.1139/gen-2021-0052","DOIUrl":"https://doi.org/10.1139/gen-2021-0052","url":null,"abstract":"The avian pectoralis muscle demonstrates plasticity with regard to size, so that temperate birds facing winter conditions or birds enduring a migration bout tend to have significant increases in the size and mass of this tissue due to muscular hypertrophy. Myonuclear domain (MND), the volume of cytoplasm a myonuclei services, in the pectoralis muscle of birds seems to be altered during thermal stress or changing seasons. However, there is no information available regarding muscle DNA content or ploidy level within the avian pectoralis. Changes in muscle DNA content can be used in this tissue to aid in size and mass changes. Here, we hypothesized that long-distance migrants or temperate residents would use the process of endoreduplication to aid in altering muscle size. Mostly contradictory to our hypotheses, we found no differences in the mean muscle DNA content in any of the 62 species of birds examined in this study. We also found no correlations between mean muscle DNA content and other muscle structural measurements, such as the number of nuclei per millimeter of fiber, myonuclear domain, and fiber cross-sectional area. Thus, while avian muscle seems more phenotypically plastic than mammalian muscle, the biological processes surrounding myonuclear function may be more closely related to those seen in mammals.","PeriodicalId":12809,"journal":{"name":"Genome","volume":"65 4","pages":"219-227"},"PeriodicalIF":3.1,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39684798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genes and proteins associated with ribeye area and meat tenderness in a commercial Nellore cattle population. 内洛尔牛商业种群中与肋眼面积和肉嫩度相关的基因和蛋白质。

IF 3.1 3区生物学

Genome Pub Date : 2022-04-01 Epub Date: 2021-12-03 DOI: 10.1139/gen-2020-0163

Gabriela B Frezarim, Larissa F S Fonseca, Bruna M Salatta, Danielly B S Silva, Tiago Bresolin, Leonardo de Oliveira Seno, Alexéia Barufatti, Jesus Aparecido Ferro, Lucia Galvão Albuquerque

{"title":"Genes and proteins associated with ribeye area and meat tenderness in a commercial Nellore cattle population.","authors":"Gabriela B Frezarim, Larissa F S Fonseca, Bruna M Salatta, Danielly B S Silva, Tiago Bresolin, Leonardo de Oliveira Seno, Alexéia Barufatti, Jesus Aparecido Ferro, Lucia Galvão Albuquerque","doi":"10.1139/gen-2020-0163","DOIUrl":"https://doi.org/10.1139/gen-2020-0163","url":null,"abstract":"Despite several studies on genetic markers and differentially expressed genes related to ribeye area (REA) and tenderness traits in beef cattle, there is divergence in the results regarding the genes associated with these traits. Thirteen genes associated with or exhibiting biological functions that might influence such phenotypes were included in this study. A total of five genes for REA (IGF-1, IGF-2, MSTN, NEDD4, and UBE4A) and eight genes for meat tenderness (CAPN1, CAPN2, CAST, HSPB1, DNAJA1, FABP4, SCD, and PRKAG3) were selected from previous studies on beef cattle. Genes and their respective proteins expression were validated in a commercial population of Nellore cattle using quantitative real-time PCR (RT-qPCR) and advanced mass spectrometry (LC/MS-MS) techniques, respectively. The MSTN gene was upregulated in animals with low REA. The CAPN1, CAPN2, CAST, HSPB1, and DNAJA1 genes were upregulated in animals with tough meat. The proteins translated by these genes were not differentially expressed. Our results confirm the potential of some of the studied genes as biomarkers for carcass and meat quality traits in Nellore cattle.","PeriodicalId":12809,"journal":{"name":"Genome","volume":"65 4","pages":"229-240"},"PeriodicalIF":3.1,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39956974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

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