Theresa Gruber, Katerina Fliegerová, Georg Terler, Reinhard Resch, Qendrim Zebeli, Thomas Hartinger
{"title":"Mixed ensiling of drought-impaired grass with agro-industrial by-products and silage additives improves the nutritive value and shapes the microbial community of silages","authors":"Theresa Gruber, Katerina Fliegerová, Georg Terler, Reinhard Resch, Qendrim Zebeli, Thomas Hartinger","doi":"10.1111/gfs.12669","DOIUrl":"10.1111/gfs.12669","url":null,"abstract":"<p>Droughts lead to reduced biomass production and unfavourable nutrient composition in grassland. As an alleviation, yet unexploited strategy, mixed ensiling of grass with agro-industrial by-products may improve the ensilability and nutritive value of drought-impaired grassland. This study investigated first whether mixed ensiling of drought-impaired grass with either sugar beet pulp (SBP), wheat gluten feed (WGF) or brewers' grains (BG) has a beneficial impact on chemical composition, fermentation characteristics, in vitro gas production (GP) and physically effective neutral detergent fibre (peNDF) of silages. Secondly, it was tested whether the application of anaerobic fungi culture supernatant (AF), mixed ruminal fluid (RF) or lactic acid bacteria (LAB) provides further advantages. Additionally, the microbial community composition was evaluated in selected silages. All silages showed satisfying conservation characteristics with high lactic acid levels and low dry matter losses, and peNDF values typically found for conserved forages. Mixed ensiling with BG substantially increased the crude protein concentration, whereas SBP increased the total degradability and WGF enhanced both. The further addition of fresh AF resulted in the overall highest lactic acid levels, especially in SBP-based silages, but without changes in in vitro GP. The in vitro GP was higher with RF, particularly in mixed silages, suggesting an improved degradability. The LAB-treated silages showed lower pH compared to controls, but had no impact on in vitro GP kinetics. Concluding, mixed ensiling holds potential to produce high-quality silages from drought-impaired grassland. The further addition of silage additives can be useful for certain substrates, but appeared not mandatory.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 2","pages":"179-197"},"PeriodicalIF":2.4,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gfs.12669","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140299906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Estefanía Oyharçabal, Fernanda Covacevich, Ingrid Bain, Claudina Soledad Acuña, Germán Darío Berone
{"title":"Cattle dry manure fertilization increases forage yield of grass-legume mixtures, while maintaining the legume proportion and root-associated microbiota","authors":"Estefanía Oyharçabal, Fernanda Covacevich, Ingrid Bain, Claudina Soledad Acuña, Germán Darío Berone","doi":"10.1111/gfs.12656","DOIUrl":"10.1111/gfs.12656","url":null,"abstract":"<p>The aim of this study was to find ways to improve the forage yield of grass-legume mixtures without compromising soil biodiversity. In Argentinean Patagonia, the effects of applying cattle dry manure (M) and urea (U) (0, 60, 120, or 240 kg N ha<sup>−1</sup> year<sup>−1</sup>) were assessed on herbage production of irrigated fescue-lotus mixtures, as well as on the activity/abundance of autochthonous arbuscular mycorrhizal fungi and N-fixing rhizobium bacteria. We hypothesised that manure has advantages over urea in increasing forage yields while maintaining the proportion of legumes and root-associated microbiota. The 120 U, 240 U, and 240 M resulted in the greatest forage production; however, yield varied depending on the source applied. The high productivity of the 120 U and 240 U was probably due to the fast grass growth immediately after fertilization, which resulted in a depressed growth of the legume. The high yield of the swards fertilized with 240 M was probably due to slight and delayed growth of grass without legume yield decline. The highest radiation interception was found in swards with a low legume proportion, suggesting a light competition from grass fertilized with the highest urea doses, which were also consistent with the highest <i>N</i> and <i>P</i> nutritional status. The microbial activity/abundance were not affected by fertilization, but the final number of nodules was positively associated with the legume proportion. In conclusion, manure fertilization increased forage yield of the mixtures, while preserving the legume proportion and the root-associated microbiota. Our findings aid in reducing synthetic-N fertilizers applied in pasture-based livestock systems.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 2","pages":"281-293"},"PeriodicalIF":2.4,"publicationDate":"2024-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Root-zone enrichment of soil organic carbon and nitrogen under grazing and other land uses in a humid-temperate region","authors":"Alan Franzluebbers","doi":"10.1111/gfs.12665","DOIUrl":"10.1111/gfs.12665","url":null,"abstract":"<p>Grazing lands can be considered a conservation land use providing value to farmers and society. An underappreciated value of these lands may be in C and N storage and cycling. However, soil organic C (SOC) and total soil N (TSN) storage under humid-temperate zone pastures have not been well characterized. A survey of 31 farms in Virginia USA characterized SOC and TSN depth distributions under a diversity of grassland management scenarios, as well as in comparison with long-term no-till cropland and woodland uses on the same farms. Root-zone enrichment calculations separated management-controlled SOC and TSN stocks from a baseline condition characterized at 30-cm depth. Total stock of SOC at 0–30-cm depth varied from 46 to 88 Mg C ha<sup>−1</sup> (5%–95% range from 304 soil profiles) across all land uses. Root-zone enrichment of SOC was maximized under mature pastures (≥20 years old) at 38.3 ± 1.6 Mg C ha<sup>−1</sup>, which was not different from that under woodland (39.8 ± 1.2 Mg C ha<sup>−1</sup>), but was greater than under no-till cropland (28.3 ± 1.3 Mg C ha<sup>−1</sup>) and conventional-till cropland (15.1 ± 5.1 Mg C ha<sup>−1</sup>). Root-zone enrichment of TSN was optimized at stocking rate of ~1 Mg live weight ha<sup>−1</sup>, but was not affected by stocking method, N fertilization history, or low levels of hay feeding. These results suggest that grazed pastures in Virginia are storing significant amounts of SOC and TSN, with at least half due to management-induced compared with pedogenic-controlled accumulation.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 2","pages":"265-280"},"PeriodicalIF":2.4,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gfs.12665","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140200582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jeferson Garcia Augusto, Claudia Cristina Paro de Paz, Gabriela Geraldi Mendonça, Mara Regina Moitinho, Leonardo Sartori Menegatto, Denizart Bolonhezi, Márcia Saladini Vieira Salles, Flávia Fernanda Simili
{"title":"Integrated crop-livestock versus conventional systems: Effects on the chemical and physical characteristics of an Oxisol","authors":"Jeferson Garcia Augusto, Claudia Cristina Paro de Paz, Gabriela Geraldi Mendonça, Mara Regina Moitinho, Leonardo Sartori Menegatto, Denizart Bolonhezi, Márcia Saladini Vieira Salles, Flávia Fernanda Simili","doi":"10.1111/gfs.12664","DOIUrl":"10.1111/gfs.12664","url":null,"abstract":"<p>Studies have reported improvements in soil quality in integrated crop-livestock systems (ICLS) when compared to conventional systems. The hypothesis of this study was that ICLS improve Oxisol quality due to the use of intercropping, adding pasture and cattle to the systems. The aim of the study was to evaluate the chemical and physical characteristics of an Oxisol and pasture production in the integrated system versus conventional system. We compared two conventional systems: crop system (maize grain production) and livestock system (beef cattle on single pasture) and, four ICLS: maize plus Marandu palisade grass sown simultaneously without herbicide (ICLS-1); maize plus Marandu palisade grass sown simultaneously with herbicide (ICLS-2); maize plus Marandu palisade grass in lagged sowing (ICLS-3); and maize plus Marandu palisade grass sown simultaneously in maize rows and inter-rows with herbicide (ICLS-4). A randomized block design with six treatments and three replicates was used. The results suggest improvements in soil quality, including soil organic carbon (ICLS-4), total nitrogen, and nitrogen stocks (ICLS-1, ICLS-2, and ICLS-4). The intercropping technique influenced pasture production and soil quality during the implementation of integrated systems. The lowest soil strength was obtained for the crop system compared to systems with grazing animals but there were no changes in the soil physical characteristics that could compromise the production system.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 2","pages":"254-264"},"PeriodicalIF":2.4,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140152029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hui-min Zhang, Ming-jiu Wang, John Hampton, Phil Rolston, Richard Lucas, Zhong-hui Ti
{"title":"Morphology of flower bud differentiation and floral organ specialization in Caucasian clover","authors":"Hui-min Zhang, Ming-jiu Wang, John Hampton, Phil Rolston, Richard Lucas, Zhong-hui Ti","doi":"10.1111/gfs.12658","DOIUrl":"10.1111/gfs.12658","url":null,"abstract":"<p>Flower bud differentiation is the commencement of plant reproductive development. Flower organ specialization follows flower bud differentiation. This biological mechanism controls whether plants reproduce successfully via seed. Caucasian clover (<i>Trifolium ambiguum</i>) is used in mixed temperate pastures. However, its low natural seed setting rate contributes to a low seed yield. This study aimed to elucidate how the reproductive biology of Caucasian clover could affect seed production. Caucasian clover flower bud differentiation and flower organ development were systematically examined. Flower bud differentiation has six consecutive stages: pre-differentiation, differentiation of the inflorescence, the floret, the perianth, the stamen, and finally the pistil primordium. The anther has two chambers. Pollen grains have six development phases: the tetraspore, early-uninucleate, late-uninucleate, vegetative cell and germ-cell form, binucleate, and maturation phase. The microspore tetrad is tetrahedral, and the mature pollen grains are bicellular. Pollen grain viability was found 35%. The Caucasian clover monocarpelate ovary contains 1–2 ovules that are covered with a 2-layer integument. The ovule is anatropous. The embryo sac development type is a polygonum. The floral formula is “⚥↑K<sub>(5)</sub> C<sub>1+1+2</sub> A<sub>(9)+1</sub> <span>G</span><sub>(1:1:1−2)</sub>”. These genetic traits of two pollen sacs and low viability pollen grains may be responsible for the low natural seed setting. Poor seed setting may also be due to embryo sac dysplasia that leads to ovule sterility. Therefore, the breeding aim should be to improve pollen viability, to decrease embryo sac dysplasia and to reduce embryo abortion that could be targeted for genetic improvement to enhance seed yields.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 3","pages":"381-391"},"PeriodicalIF":2.7,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gfs.12658","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140073925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Parmeshwor Aryal, Lynn E. Sollenberger, José C. B. Dubeux Jr.
{"title":"Nitrogen application during rhizoma peanut shoot emergence affects planting-year nitrogen fixation","authors":"Parmeshwor Aryal, Lynn E. Sollenberger, José C. B. Dubeux Jr.","doi":"10.1111/gfs.12657","DOIUrl":"10.1111/gfs.12657","url":null,"abstract":"<p>Rhizoma peanut (<i>Arachis glabrata</i> Benth.; RP) is an important perennial forage legume in the US Gulf Coast region, but it is vegetatively propagated from rhizomes and relatively slow to establish. There are reports that N fertilization enhances RP establishment, but other evidence suggests plant N content and establishment rate may not benefit. Understanding the effect of N fertilizer on RP biological N<sub>2</sub> fixation will help elucidate this response. Our objective was to determine the effect of RP genotype and N fertilization during shoot emergence on N<sub>2</sub> fixation and N accumulation during the year of planting. Replicated field experiments were conducted in adjoining fields; one was planted in 2016 and the other in 2017. Treatments were the factorial combinations of two RP entries (decumbent germplasm Ecoturf and upright cultivar ‘UF Tito’) and three N rates (0, 40, and 80 kg N ha<sup>−1</sup> applied 10 wk after planting; 1% of total N as <sup>15</sup>N) arranged in a randomized complete block design with four replications. Across N rates, upright cultivar UF Tito generally had greater N concentration and content in aboveground and belowground plant parts compared with decumbent germplasm Ecoturf. Nitrogen fertilization generally reduced the percentage of N derived from atmosphere and the amount of fixed N (BNF) during the year of planting, but it did not affect overall N concentration or content. These data suggest RP increased soil N uptake to compensate for reduced BNF following N fertilization, indicating starter N application had no measurable benefit for legume establishment under these conditions.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 3","pages":"353-365"},"PeriodicalIF":2.7,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139956392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Growth responses to waterlogging stress among cool-season grass species","authors":"Ross C. Braun, Aaron J. Patton","doi":"10.1111/gfs.12655","DOIUrl":"10.1111/gfs.12655","url":null,"abstract":"<p>Urban grasslands span climates and topography in soils with variable water infiltration and drainage rates that result in occasional waterlogging stress, while data on grass species tolerance to waterlogging stress is scant. Whole plant responses to waterlogging stress among cool-season grass species were quantified in a controlled environment. The following grasses were grown in well-drained vs. waterlogged soil for 55 d: strong creeping red fescue (<i>Festuca rubra</i> ssp. <i>rubra</i>), slender creeping red fescue (<i>F</i>. <i>rubra</i> ssp. <i>littoralis</i>), Chewings fescue (<i>F</i>. <i>rubra</i> ssp. <i>commutata</i>), hard fescue (<i>F. brevipila</i>), tall fescue (<i>F</i>. <i>arundinacea</i> syn. <i>Schedonorus arundinaceus</i>), Kentucky bluegrasses (<i>Poa pratensis</i>), annual bluegrass (<i>P. annua</i>), rough bluegrass (<i>P. trivialis</i>), creeping bentgrass (<i>Agrostis stolonifera</i>), perennial ryegrass (<i>Lolium perenne</i>), and alkaligrass (<i>Puccinellia distans</i>). Five cultivars of each fine fescue (<i>Festuca</i> spp.) taxon were included for comparison. When grown in waterlogged soil compared to well-drained conditions, relative differences generally ranged from −3% to −26% (shoots) and −13% to −33% (roots) for creeping bentgrass, tall fescue, and Kentucky bluegrass indicating higher waterlogging stress tolerance. The relative differences ranged from −18% to −43% in shoots and −3% to −34% in roots for annual bluegrass and perennial ryegrass indicating fair performance under waterlogging stress. Fine fescues, rough bluegrass, and alkaligrass exhibited the poorest performance during waterlogging stress with plant responses ranging from −12% to −64% (shoots) and −17% to −73% (roots). Negative whole plant responses among cultivars of four fine fescue taxa were similar. The selection of grasses tolerant to waterlogging stress will be important in developing resilient landscapes.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 3","pages":"464-475"},"PeriodicalIF":2.7,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/gfs.12655","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139909705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Utilizing treated wastewater for pasture irrigation: Effects on productivity, plant community structure and soil properties","authors":"Renana Lavi, Avi Bar-Massada, Guy Dovrat","doi":"10.1111/gfs.12652","DOIUrl":"10.1111/gfs.12652","url":null,"abstract":"<p>Surplus wastewater accumulates in winter and requires a discharging solution. In our study we examined the effects of using treated wastewater (TWW) as supplemental pasture irrigation in winter. To test the effect of TWW irrigation on the vegetation and soil, 10 grazing prevention exclosures were established at Ramat-Hanadiv Park, Israel, with half of them irrigated with TWW. Soil and vegetation were sampled during 2 years of irrigation and 1 year after cessation of irrigation. Vegetation composition, species richness, and productivity were sampled at the peak of the growing season and various indices of herbage quality were measured. Our results show that irrigation with wastewater increased herbage production by an average of 38% and total pasture protein production by 9% relative to the control subplots. Conversely, TWW irrigation decreased plant protein content while increasing herbage fibre content and lowering its digestibility. Over a period of 2 years, species richness decreased in the irrigated subplots. Most of the soil indices examined were not affected by irrigation, but in the irrigation subplots, sodium values and the sodium adsorption-ratio were higher than in areas without irrigation. Additional resources in winter enabled the plants to grow rapidly and increased interplant competition. Changes in vegetation composition were revealed through functional group reshuffling and species richness decline. The results of our study show that wastewater irrigation causes an increase in productivity but a decrease in forage quality. At the pastoral system level, the significant increase in productivity leads to increased availability of protein and herbage for animals.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 2","pages":"228-238"},"PeriodicalIF":2.4,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139752300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aimee-Louise Craig, Alan W. Gordon, Conrad P. Ferris
{"title":"Removal of autumn-growth herbage using sheep: Effects on yield and quality of first cut silage and subsequent cow performance","authors":"Aimee-Louise Craig, Alan W. Gordon, Conrad P. Ferris","doi":"10.1111/gfs.12654","DOIUrl":"10.1111/gfs.12654","url":null,"abstract":"<p>Grass which grows during the autumn is perceived by some farmers to have a negative impact on the nutritive value of silage produced the following spring. The impact of removing herbage in the autumn using sheep, on silage yield and quality the following spring, and on performance of cows offered these silages, was investigated in two experiments. Following harvest of third-cut silage in September, a grass sward was split into blocks which were either grazed by sheep during November and December or left ungrazed. Herbage was harvested and ensiled the following May and offered to late-lactation Holstein cows in a two-period balanced change-over design feeding experiment comprising two 28-d periods. In Experiment 1, silage quality was unaffected by autumn grazing treatment but in Experiment 2, silage from swards grazed in autumn had a higher metabolizable energy concentration (0.5 MJ kg<sup>−1</sup> dry matter [DM]; <i>p</i> = .016). In Experiment 1, intakes were unaffected, while cows offered silage from the grazed sward (GS) had a greater milk (0.8 kg d<sup>−1</sup>; <i>p</i> < .001) and protein yield (0.03 kg d<sup>−1</sup>; <i>p</i> = .014) than cows offered silage from the ungrazed sward, but fat plus protein yield was unaffected. In Experiment 2, cows offered GS had greater DM intake (1.5 kg d<sup>−1</sup>; <i>p</i> < .001) and fat yield (0.15 kg d<sup>−1</sup>; <i>p</i> = .047), but fat plus protein yield was not significantly different between treatments. In conclusion, winter grazing using sheep has potential to improve silage quality, but with marginal benefits on individual cow performance.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 3","pages":"438-448"},"PeriodicalIF":2.7,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139752298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Igor L. Bretas, Harley D. Naumann, Jose C. B. Dubeux Jr, Joao M. B. Vendramini, Hiran M. S. Silva, Esteban Rios, Luana M. D. Queiroz, Thomas P. Mawhinney
{"title":"Herbage accumulation, nutritive value, and fatty acid profile of bermudagrass cultivars grown in different latitudes","authors":"Igor L. Bretas, Harley D. Naumann, Jose C. B. Dubeux Jr, Joao M. B. Vendramini, Hiran M. S. Silva, Esteban Rios, Luana M. D. Queiroz, Thomas P. Mawhinney","doi":"10.1111/gfs.12650","DOIUrl":"10.1111/gfs.12650","url":null,"abstract":"<p>The geographical distribution of bermudagrass cultivars is limited by freezing events (<0°C), commonly associated with higher latitudes in the northern hemisphere. The lipid composition in the plant cell membranes is critical to promote persistence under freezing conditions. This study evaluated herbage accumulation (HA), nutritive value, and lipid biosynthesis of different bermudagrass cultivars in Ona, FL (27°26' N 82°55' W) and Marianna, FL (30<sup>o</sup>52' N 85<sup>o</sup>11' W), in 2 years. Treatments were five bermudagrass cultivars (Coastal, Tifton 44, Tifton 85, Jiggs, and Mislevy) distributed in a randomized complete block design with four replicates. HA was greater in Marianna than in Ona for all cultivars (<i>p</i> < 0.05). Mislevy and Jiggs had greater HA in Marianna than the others (<i>p</i> < 0.05), while all cultivars were similar in Ona (<i>p</i> > 0.05). There was no difference between locations or among the cultivars for crude protein and neutral detergent fibre, but bermudagrass cultivars had greater in vitro digestibility of organic matter (IVDOM) in Marianna than in Ona (<i>p</i> < 0.05). The concentration of unsaturated fatty acids increased in the northern latitude (Marianna; <i>p</i> < 0.05). Coastal had greater unsaturated fatty acid concentration than other cultivars (<i>p</i> < 0.05). Geographical location affects HA, IVDOM, and unsaturated fatty acid concentration of bermudagrass cultivars. The unsaturated fatty acid concentration plays a key role in acclimating bermudagrass to different thermal environments. Coastal showed great potential to tolerate chilling stress in Florida. Further studies may be developed to identify promising molecular markers for cold tolerance in bermudagrass.</p>","PeriodicalId":12767,"journal":{"name":"Grass and Forage Science","volume":"79 2","pages":"318-327"},"PeriodicalIF":2.4,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139752242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}