Glycoconjugate Journal最新文献_第3页

Identification of mitochondrial associated genes as diagnostic biomarkers for basal cell carcinoma: Comprehensive bioinformatics analysis and experimental validation. 鉴定线粒体相关基因作为基底细胞癌的诊断生物标志物：综合生物信息学分析和实验验证。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2026-02-12 DOI: 10.1007/s10719-026-10209-1

Zuojiao Xu, Chunjun Yang

{"title":"Identification of mitochondrial associated genes as diagnostic biomarkers for basal cell carcinoma: Comprehensive bioinformatics analysis and experimental validation.","authors":"Zuojiao Xu, Chunjun Yang","doi":"10.1007/s10719-026-10209-1","DOIUrl":"10.1007/s10719-026-10209-1","url":null,"abstract":"The aim of our research was to explore and validate the diagnostic value of mitochondria-related genes (MRGs) in basal cell carcinoma (BCC). The differentially expressed MRGs in BCC were identified based on the GSE39612, GSE42109 and GSE7553 training sets and the GSE53462 validation set, and the PPI network was drawn. The potential signature genes in BCC were screened by LASSO, RF and SVM-RFE. Besides, the performance of the signature genes was evaluated and a nomogram was constructed. Moreover, immunoinfiltration, drug prediction and transcriptional regulation of these signature genes were also analyzed. Finally, qRT-PCR was employed to measure the expression of signature genes in clinical tissues and BCC cells. 1699 DEGs were obtained from the training set. Then, taking the intersection of DEGs, WGCNA module genes and MRGs, 36 DEGs-MRGs were obtained. Six potential signature genes in BCC, including ACADL, BCL2L2, CHCHD7, LDHB, NT5DC2, and PDK4, were obtained through LASSO, RF, and SVM-RFE. Nomogram and validation analysis confirmed that these 6 genes have ideal predictive power in BCC. In addition, 6 signature genes were significantly associated with macrophages and interacted with pyruvic acid, sodium chromate, perfluoroheptanoic acid and other drugs. Finally, qRT-PCR analysis showed that the expression trend of these 6 signature genes in clinical tissues and BCC cells was consistent with the results of bioinformatics analysis. ACADL, BCL2L2, CHCHD7, LDHB, NT5DC2 and PDK4 are potential diagnostic signature genes of BCC.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"10"},"PeriodicalIF":3.0,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total plasma N-glycomic patterns of COVID-19 disease. COVID-19疾病的总血浆n -糖糖型。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2026-01-20 DOI: 10.1007/s10719-025-10201-1

Georgia Elgood-Hunt, Jack Cheeseman, Richard A Gardner, Thomas Sénard, Paulina A Urbanowicz, Alejandro A Garcia Leon, Cormac McCarthy, Marco P Monopoli, Daryl L Fernandes, Craig P Thompson, Oleg A Mayboroda, Patrick W G Mallon, Daniel I R Spencer

{"title":"Total plasma N-glycomic patterns of COVID-19 disease.","authors":"Georgia Elgood-Hunt, Jack Cheeseman, Richard A Gardner, Thomas Sénard, Paulina A Urbanowicz, Alejandro A Garcia Leon, Cormac McCarthy, Marco P Monopoli, Daryl L Fernandes, Craig P Thompson, Oleg A Mayboroda, Patrick W G Mallon, Daniel I R Spencer","doi":"10.1007/s10719-025-10201-1","DOIUrl":"10.1007/s10719-025-10201-1","url":null,"abstract":"Total plasma N-glycans alter in disease states, with few studies focused on COVID-19. A discovery cohort of 310 COVID-19 patients, replicated with 97 COVID-19 patients and tested with 100 COVID-19 patients, was used to unearth the N-glycans capable of distinguishing infection, as well as prognosis of intensive care unit (ICU) admission and mortality. All significant bisected glycans were decreased in patients compared to controls, whilst fucosylated tri-antennary glycans and sialylated tetra-antennary glycans were increased in patients. For both those admitted to the ICU and those who died, Peak 61 (A4G4S4F) was elevated in the more severe disease course, while Peak 29 (FA2G2S2) was lowered. Pinpointing specific glycosylation changes has alluded to a potential story of glycoprotein pathways in response to SARS-CoV-2 infection. This study could be further explored through deriving the glycoproteins associated with the glycans of interest and the glycosylation changes experienced on these proteins.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"9"},"PeriodicalIF":3.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12819545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146009651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and structural elucidation of immunomodulatory oligosaccharides from Trillium tschonoskii. 黄芩免疫调节寡糖的分离与结构分析。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2026-01-12 DOI: 10.1007/s10719-025-10204-y

Juan Song, Si Liu, Meng Sun, Guangzhong Tu, Zhiqiang Zhang, Yue Liu, Yi Nan, Guobin Shen, Xiaojuan Chen, Haizhen Liang, Bin Liu, Baiping Ma

{"title":"Isolation and structural elucidation of immunomodulatory oligosaccharides from Trillium tschonoskii.","authors":"Juan Song, Si Liu, Meng Sun, Guangzhong Tu, Zhiqiang Zhang, Yue Liu, Yi Nan, Guobin Shen, Xiaojuan Chen, Haizhen Liang, Bin Liu, Baiping Ma","doi":"10.1007/s10719-025-10204-y","DOIUrl":"10.1007/s10719-025-10204-y","url":null,"abstract":"Six oligosaccharides with the degree of polymerization (DP) 2 to 7 were isolated from the active fraction of Trillium tschonoskii using a high-temperature semi-preparative porous graphitic carbon (PGC) column based on high-performance liquid chromatography coupled with a charged aerosol detector and vanquish fraction collector (HPLC-CAD-VFC) system. The structures of DP2 - 7 were determined as maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, and maltoheptaose with (1→4)-α-D-glucopyranose residues by relative quantitative 1H nuclear magnetic resonance (qHNMR) spectroscopy with anomeric protons integration and NMR structural-reporter-group resonances. The immunomodulatory activities of this series of maltooligosaccharides (MOS) were tested on RAW264.7 cells. All samples on cells had no cytotoxicity, and the oligosaccharide fraction (TOS1) could increase the phagocytic capacity and production of nitric oxide (NO) and cytokines to a much higher level than monomers. In particular, maltopentaose (DP5) showed a relatively higher capacity for stimulating proliferation, NO, and cytokine production than other purified oligosaccharides. The immunomodulatory activity of MOS investigated in this study is beneficial for utilizing MOS as a functional ingredient in novel product development.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"8"},"PeriodicalIF":3.0,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review on macrophage polarization during visceral leishmaniasis and impact of glycoprotein. 内脏利什曼病巨噬细胞极化及糖蛋白影响的研究进展。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2026-01-09 DOI: 10.1007/s10719-025-10206-w

Nayanika Datta, Sajal Dasmahapatra, Madhusri Pramanik, Santanu Kar Mahapatra

{"title":"Review on macrophage polarization during visceral leishmaniasis and impact of glycoprotein.","authors":"Nayanika Datta, Sajal Dasmahapatra, Madhusri Pramanik, Santanu Kar Mahapatra","doi":"10.1007/s10719-025-10206-w","DOIUrl":"10.1007/s10719-025-10206-w","url":null,"abstract":"Visceral Leishmaniasis is one of the neglected vector-borne tropical disease escalating resistance against available drugs which have toxicity beyond therapeutic level leading to the requirement of alternative therapy. Macrophages, the primary cells of the immune system, being the host cells for the Leishmanial parasite undergo functional phenotypic changes based on its infected or uninfected states leading to release of specific cytokines. The M2 functional phenotype is suitable for parasite survival, while, M1 phenotype boosts the immune system leading to release of superoxide responsible for parasite clearance. Natural isolates i.e. peptides and glycoproteins isolated from various origin have been found to possess antileishmanial activities enhancing the chances to be used as a curative agent. Among them, plant glycoproteins have been found to possess immunostimulatory, antibacterial, antifungal, antiviral, anticancerous, as well as, antileishmanial activity attracting focus of researchers towards it as an alternating treating compound. This review highlights the drawbacks of existing drugs, route of development of the disease, mechanism of macrophage polarization as well as the overview of different isolated peptides and glycoproteins acting as an antileishmanial agent, having immunomodulating activity and an indication that glycoproteins can be presumed as an alternative therapy against the fatal noxious disease, if left untreated.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"7"},"PeriodicalIF":3.0,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145943318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating plasma protein-centric multi-omics to evaluate the causal effect of glycosylation on the risk of cancer. 整合以血浆蛋白为中心的多组学来评估糖基化对癌症风险的因果影响。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2026-01-06 DOI: 10.1007/s10719-025-10207-9

Zhi Geng, Kangwei Qi, Long Yu, Yuanyuan Ruan, Jianxin Gu, Shushu Song

{"title":"Integrating plasma protein-centric multi-omics to evaluate the causal effect of glycosylation on the risk of cancer.","authors":"Zhi Geng, Kangwei Qi, Long Yu, Yuanyuan Ruan, Jianxin Gu, Shushu Song","doi":"10.1007/s10719-025-10207-9","DOIUrl":"10.1007/s10719-025-10207-9","url":null,"abstract":"Glycosylation is a crucial post-translational modification, and numerous studies have reported its significant role in cancer progression. Nevertheless, no study has comprehensively analyzed the causal effect of glycosylation on the risk of cancer till now. Herein, we identified 32 SNPs of glycosylation-related genes (GRGs) that correlated with the risk of 8 kinds of cancer by summary-statistics-based mendelian randomization (SMR) analysis for genome-wide association study (GWAS) data. Next, the heterogeneity in dependent instrument (HEIDI) test and colocalisation analysis were utilized to verify the heterogeneity and consistency of SMR results. Further fine-mapping of causal gene set (FOCUS) analysis based on transcriptome-wide association study (TWAS) data showed that rs9810189 of ST6GAL1 and rs223489 of MANBA were negatively correlated with the risk of breast cancer and squamous cell carcinoma, respectively. Moreover, the MANBA protein in blood plasma also exhibited a probably negative causal effect on squamous cell carcinoma according to two-sample MR analyses for protein quantitative trait locus (pQTLs). In addition, single-cell RNA sequencing analysis and Kaplan-Meier plot analysis were performed to evaluate the potential role of GRGs in corresponding cancer prioritized by the above MR analysis. Finally, we attempted to illustrate the function of glycosyltransferases and investigate the druggable status of GRGs. Together, our study comprehensively analyzed the causal effect of glycosylation on cancer risk and identified potential strategies for cancer treatment.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"6"},"PeriodicalIF":3.0,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145911201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SPR interaction analysis of GM3 glycan with the integrin α5β1 head domain. GM3聚糖与整合素α5β1头部结构域的SPR相互作用分析。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2026-01-06 DOI: 10.1007/s10719-025-10205-x

Shinya Hanashima, Migiwa Kishi, Katsuaki Sasaki, Yusuke Sato, Michio Murata

{"title":"SPR interaction analysis of GM3 glycan with the integrin α5β1 head domain.","authors":"Shinya Hanashima, Migiwa Kishi, Katsuaki Sasaki, Yusuke Sato, Michio Murata","doi":"10.1007/s10719-025-10205-x","DOIUrl":"10.1007/s10719-025-10205-x","url":null,"abstract":"Integrins are heterodimeric receptors involved in cell adhesion and bidirectional signaling. Ganglioside GM3 in the outer leaflet of cell membranes possibly regulates integrin activity via direct interactions; however, its specific binding mode with integrins remains unclear. Therefore, in this study, we focused on the GM3 glycan moiety, which encounters the integrin ectodomain on the cell surface, and synthesized a soluble GM3 probe without hydrocarbon chains using a chemoenzymatic approach. Binding analysis using surface plasmon resonance (SPR) indicated that the synthetic GM3 probe interacts with the integrin α5β1 ectodomain with a significantly higher affinity than the lactosylceramide probe. Saturation transfer difference (STD) NMR spectra suggested that the integrin α5β1 ectodomain interacts with N-acetylneuraminic acid (Neu5Ac) at the GM3 terminal and α2-3Gal linkage. Notably, RGD peptide, an integrin ligand interacting with the heterodimer interface, competed with GM3 to bind to integrin α5β1 on the SPR sensor chip. Consistent with these results, the GM3-binding site predicted by Chai-1 partially overlapped with the RGD peptide-binding groove on integrin α5β1; however, the binding mode was different. RGD peptide bridged the α and β subunits of the integrin head moiety, whereas the GM3 probe found at the cleft between the subunits. The Neu5Ac-Gal moiety primarily interacts with the metal ion-dependent adhesion site and nearby residues in the β1 subunit. Overall, our findings suggest that gangliosides directly interact with integrin α5β1 at a previously unknown binding site, revealing a novel regulatory mechanism for the integrin activity.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"5"},"PeriodicalIF":3.0,"publicationDate":"2026-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145911181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of IgG N-Glycan patterns in adult patients with immune thrombocytopenia and healthy individuals. 成年免疫性血小板减少症患者和健康人IgG n -聚糖模式的评估。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2026-01-04 DOI: 10.1007/s10719-025-10199-6

Ena Ranković, Natali Nakić Bedeković, Frano Vučković, Irena Trbojević-Akmačić, Maja Pučić-Baković, Gordan Lauc, Dražen Pulanić

{"title":"Assessment of IgG N-Glycan patterns in adult patients with immune thrombocytopenia and healthy individuals.","authors":"Ena Ranković, Natali Nakić Bedeković, Frano Vučković, Irena Trbojević-Akmačić, Maja Pučić-Baković, Gordan Lauc, Dražen Pulanić","doi":"10.1007/s10719-025-10199-6","DOIUrl":"10.1007/s10719-025-10199-6","url":null,"abstract":"Immune thrombocytopenia (ITP) is an acquired autoimmune disorder characterized by a platelet count less than 100,000/μL caused by increased immune-mediated destruction of platelets and, in some cases, impaired platelet production in the bone marrow. Previous studies have shown that in various pathological conditions, such as inflammatory and autoimmune diseases, changes in the composition of glycans occur, and that these differences have a significant impact on the progression of various diseases. We examined changes in the glycosylation of immunoglobulin G (IgG) in ITP in a prospective study that was conducted in the Division of Hematology at the University Hospital Centre Zagreb from 2015 to 2021. The study included 56 adult patients with ITP and 26 healthy controls. Liquid chromatography based on hydrophilic interactions was used for glycan analysis. Patients with ITP had a statistically significantly lower proportion of sialylated glycans and an increased proportion of agalactosylated structures. This could be associated with reduced anti-inflammatory activity of IgG and increased complement activation, all of which result in increased destruction of platelets. Analysis of IgG glycosylation in patients with ITP could have significant implications for understanding the pathophysiology of the disease and potentially become a useful biomarker for the diagnosis of ITP.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"4"},"PeriodicalIF":3.0,"publicationDate":"2026-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145896437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass spectrometric profiling reveals alterations in N-Glycans and O-Glycans in Tay-Sachs disease under Autophagy-Induced conditions. 质谱分析揭示了自噬诱导条件下Tay-Sachs病n -聚糖和o -聚糖的变化。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2025-12-29 DOI: 10.1007/s10719-025-10203-z

Melike Can, Hande Basirli, Chunsheng Jin, Niclas G Karlsson, Daniel Bojar, Volkan Seyrantepe

{"title":"Mass spectrometric profiling reveals alterations in N-Glycans and O-Glycans in Tay-Sachs disease under Autophagy-Induced conditions.","authors":"Melike Can, Hande Basirli, Chunsheng Jin, Niclas G Karlsson, Daniel Bojar, Volkan Seyrantepe","doi":"10.1007/s10719-025-10203-z","DOIUrl":"10.1007/s10719-025-10203-z","url":null,"abstract":"Tay-Sachs disease is a rare neurodegenerative disorder caused by mutations in the HEXA gene. The HEXA gene encodes the α-subunit of the enzyme β-hexosaminidase A, which degrades GM2 ganglioside. Previously, we identified impaired autophagy in the brains of a mouse model of Tay-Sachs disease, which exhibited neuropathological and clinical abnormalities. Moreover, we demonstrated autophagosome clearance in Tay-Sachs cells under lithium-induced conditions. Here, we further aimed to evaluate N- and O-glycan changes in these cells and examine whether glycan alterations are linked to ER stress. The profiles of N- and O-glycans were analyzed using LC-MS/MS in fibroblasts and neuroglial cells from 5-month-old Hexa-/-Neu3-/- mice and neuroglial cells from Tay-Sachs patients under lithium induction and nutrient deprivation. The expression levels of ER stress-related markers were assessed using qRT-PCR and Western blot analyses. We demonstrated higher levels of high mannose and lower levels of complex types of N-glycans, along with increased O-glycan levels in Tay-Sachs cells. Compared to control groups, we observed upregulated expression of endoplasmic reticulum (ER) stress-related markers, CHOP and ATF-6, in Tay-Sachs cells. Our study demonstrated that autophagy induction causes the degradation of accumulated high-mannose N-glycans and O-glycans, which is associated with the downregulation of ER stress-related genes in Tay-Sachs cells. Our study is the first to show this phenomenon in Tay-Sachs cells and suggests the presence of ER stress-mediated autophagy. Therefore, targeting glycans through autophagy induction could offer therapeutic benefits to patients with Tay-Sachs disease in future studies.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"3"},"PeriodicalIF":3.0,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145849412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High molecular weight fucosylated glycosaminoglycan promotes the binding of antithrombin and thrombin: a template mechanism and structure-activity relationship study. 高分子量聚焦糖胺聚糖促进抗凝血酶和凝血酶结合：模板机制及构效关系研究。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2025-12-23 DOI: 10.1007/s10719-025-10200-2

Chuang Xiao, Li Xu, Ying Cai, Zhenhua Liu, Yingnian Li, Na Gao, Jinhua Zhao

{"title":"High molecular weight fucosylated glycosaminoglycan promotes the binding of antithrombin and thrombin: a template mechanism and structure-activity relationship study.","authors":"Chuang Xiao, Li Xu, Ying Cai, Zhenhua Liu, Yingnian Li, Na Gao, Jinhua Zhao","doi":"10.1007/s10719-025-10200-2","DOIUrl":"10.1007/s10719-025-10200-2","url":null,"abstract":"Native fucosylated glycosaminoglycan (FG), a structurally unique polysaccharide with chondroitin sulfate like backbone and sulfated fucose side chains, has multiple anticoagulant mechanisms. Analyzing the interaction between FG and coagulation proteins will help to further understand its pharmacological mechanisms. Previously, we have reported that high molecular weight depolymerized FG (dFG) and unfractionated heparin (UFH) exhibit different binding models for antithrombin (AT), which is a primary natural anticoagulant in plasma by inhibiting coagulation proteases including thrombin (FIIa). In this study, the effect of dFG or UFH on the interaction between AT and FIIa was detected by biolayer interferometry. The relative AT binding affinity and AT-dependent anti-FIIa activity of dFG derivatives were measured to elucidate the structure-activity relationship. The results demonstrate that high molecular weight dFG significantly promotes the irreversible binding of FIIa and AT by a template mechanism. As the molecular weight decreases, the relative AT binding affinity of dFG decreases and molecular weight above 8.55 kDa is required for their interaction in this study. Both the fucose side chains and carboxyl groups are indispensable for dFG to bind with AT and inhibit FIIa activity. This study clarifies the mechanism and structure-activity relationship of dFG inhibiting FIIa by AT, providing references for the development of novel anticoagulant drugs based on FG and its derivatives.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"2"},"PeriodicalIF":3.0,"publicationDate":"2025-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145809708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a distinct sialic acid (KDN) and a KDN-specific aldolase in Pacific oyster. 太平洋牡蛎中独特唾液酸（KDN）及其特异性醛缩酶的鉴定。

IF 3 4区生物学

Glycoconjugate Journal Pub Date : 2025-12-19 DOI: 10.1007/s10719-025-10202-0

Zi-Xuan Hu, Jia-Yu Zhang, Jitske van Ede, Yao-Yao Zhang, Yu-Quan Li, Mattia Ghirardello, M Carmen Galan, Martin Pabst, Li Liu, Josef Voglmeir

{"title":"Identification of a distinct sialic acid (KDN) and a KDN-specific aldolase in Pacific oyster.","authors":"Zi-Xuan Hu, Jia-Yu Zhang, Jitske van Ede, Yao-Yao Zhang, Yu-Quan Li, Mattia Ghirardello, M Carmen Galan, Martin Pabst, Li Liu, Josef Voglmeir","doi":"10.1007/s10719-025-10202-0","DOIUrl":"10.1007/s10719-025-10202-0","url":null,"abstract":"Sialic acids are a diverse family of acidic sugars typically found at the terminal positions of glycan chains, mediating key physiological and pathological processes across animals - particularly vertebrates - including cell signaling and host-pathogen interactions. The distribution of sialic acids in lower animals such as mollusks, however, remains largely unresolved. Here, we report the discovery of unconjugated 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), a deaminated analogue of N-acetylneuraminic acid, in the muscle tissue of Pacific oysters (Magallana gigas). Using UPLC-ESI-MS/MS fingerprinting, we identified naturally occurring free KDN at a concentration of 1.2 ± 0.1 nmol/100 mg of oyster muscle tissue. To investigate the biosynthetic pathway, four candidate genes were identified in the M. gigas genome, and the corresponding recombinant proteins were expressed and characterized. Enzymatic assays revealed that one putative sialic acid aldolase (MgNPL) specifically catalyzes the cleavage of KDN into mannose and pyruvate. To our knowledge, this represents the first molecular evidence of KDN metabolism in mollusks and highlights both the unexpected conservation of substrate-specific aldolase activity and distinct sialic acid utilization mechanisms compared to vertebrates.","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":"43 1","pages":"1"},"PeriodicalIF":3.0,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145794172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0