Genes & genomics最新文献

{"title":"CDK9 inhibition triggers MT2A-dependent apoptosis in HCC cells.","authors":"Bilu Peng, Yuli Bao, Ziren Zhang, Jianxin Lyu, Linyong Du, Lei Zhang, Weifeng Ma","doi":"10.1007/s13258-025-01700-4","DOIUrl":"https://doi.org/10.1007/s13258-025-01700-4","url":null,"abstract":"","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parametric hypothesis testing for pathway based hierarchical structural component models.","authors":"Md Kamruzzaman, Taesung Park","doi":"10.1007/s13258-026-01749-9","DOIUrl":"https://doi.org/10.1007/s13258-026-01749-9","url":null,"abstract":"","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced molecular techniques in distinguishing monozygotic twins for forensic applications.","authors":"Hirak Ranjan Dash","doi":"10.1007/s13258-025-01732-w","DOIUrl":"10.1007/s13258-025-01732-w","url":null,"abstract":"<p><p>Despite significant advancements in the forensic DNA analysis techniques, one of the major limitations is the availability of a gold standard genetic technique to distinguishing the monozygotic (MZ) twins. NGS mediated deep sequencing approach is capable of detecting the rare post-zygotic mutations. Despite having a similarity DNA profile, tiny differences in the personality, living habits and disease susceptibility, leads to the observance of epigenetic changes. Both DNA methylation and histone acetylation have demonstrated scientific approach of distinguishing MZ twins. The stable non-coding RNAs, i.e., miRNA, circRNA, and tsRNA have been targeted in most of the studies to differentiate the MZ twins based on their differential expression. The inception of MPS technology allows the rapid sequencing of whole mtDNA genome. It allows the identification of rare mutational events as well as the heteroplasmic DNA to accurately distinguish the MZ twins. Emergence of IRs like TCRβCDR3 have also shown promise as a potential biomarker in distinguishing MZ twins. Analysis of the relatively more stable molecules by proteomics, metabolomics, and microbiome analysis have also demonstrated their MZ twins' differential capability. The present article highlights the technological advancements in the forensic differentiation of the MZ twins and their respective pros and cons.</p>","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":"471-485"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer activity of himantormione B: a new depsidone from the Antarctic lichen Himantormia lugubris, in colorectal cancer.","authors":"Hae-Jung Chae, Min-Ji Shin, Man Hyung Koo, Jong Bae Seo, Ui Joung Youn, Sung-Suk Suh","doi":"10.1007/s13258-026-01759-7","DOIUrl":"10.1007/s13258-026-01759-7","url":null,"abstract":"<p><strong>Background: </strong>Recent studies have highlighted the pharmaceutical potential of lichen extracts, particularly with respect to their anticancer properties. Lichens, symbiotic organisms composed of fungi and algae or cyanobacteria, have long been utilized in traditional medicine. Accumulating evidence from recent investigations further supports the potential therapeutic applications of lichen-derived compounds in a variety of diseases, including cancer.</p><p><strong>Objective: </strong>The study aimed to investigate the anticancer activity of himantormione B, a newly identified secondary metabolite isolated from the Antarctic lichen Himantormia lugubris, in the human colorectal cancer cell lines HCT116 and HT-29.</p><p><strong>Methods: </strong>Cell viability was assessed using an MTS assay in MRC-5, HCT116, and HT-29 cells. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunoblot analyses were performed to evaluate the expression levels of apoptosis-related genes, including BAX, BCL2, PUMA, and CASP3. To further investigate the apoptotic effects of himantormione B in HCT116 and HT-29 cells, cells were stained with Annexin V-FITC and propidium iodide (PI), followed by flow cytometric analysis (FACS). In addition, in silico ADMET analyses were conducted to determine whether himantormione B exhibits favorable drug-like properties and promising pharmacological potential.</p><p><strong>Results: </strong>Based on our results, himantormione B exerted a significant cytotoxic effect on colorectal cancer cells compared with MRC-5 normal fibroblasts. Moreover, himantormione B induced apoptosis in a concentration-dependent manner by modulating the expression of apoptosis-related genes, including BAX, BCL-2, PUMA, and CASP3. Specifically, treatment with himantormione B significantly downregulated the expression of the anti-apoptotic gene BCL-2, while concomitantly upregulating the expression of the pro-apoptotic genes BAX and PUMA. In agreement with the flow cytometric analysis demonstrating himantormione B-induced apoptosis, we further observed a dose-dependent increase in cleaved caspase-3 levels. Given that caspase-3 is a key executioner caspase that promotes apoptotic progression and suppresses cell survival, these findings collectively indicate that himantormione B induces apoptosis via activation of the intrinsic apoptotic signaling pathway.</p><p><strong>Conclusion: </strong>Taken together, the present study provides a comprehensive evaluation of the anticancer activity of himantormione B in colorectal cancer, highlighting its potential as a promising therapeutic candidate for targeted treatment strategies.</p>","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":"633-644"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of genetic mutation characteristics in 40 Chinese patients with hepatocellular carcinoma.","authors":"Shaoshao Xu, Lei Li, Dongchang Yang, Tao Liu, Binyu Liu, Qingqing Xun, Yanrong Liu","doi":"10.1007/s13258-026-01743-1","DOIUrl":"10.1007/s13258-026-01743-1","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) is notable for its marked molecular heterogeneity and poor response to systemic therapy. Targeted next-generation sequencing (NGS) has facilitated the profiling of somatic alterations in HCC. However, the interpretation of panel-based genomic features such as tumor mutational burden (TMB), copy number alterations, and candidate gene fusions remains challenging, particularly in HBV-endemic Asian populations.</p><p><strong>Objective: </strong>To explore the somatic mutation landscape of HCC using a targeted sequencing panel in a regional cohort and to descriptively assess its associations with clinicopathological characteristics.</p><p><strong>Methods: </strong>We conducted a 671-gene targeted NGS panel covering approximately 2.5 Mb of coding regions on tumor-normal paired samples from 40 patients with HCC and integrated the mutational profiles with clinicopathological data. We analyzed somatic mutations, copy number variation signals, and candidate fusion events. We performed functional enrichment analyses and comparative analyses with the Cancer Genome Atlas (TCGA) cohort. Survival analyses were conducted in an exploratory manner.</p><p><strong>Results: </strong>TP53 was the most frequently mutated gene (55%) and its mutation status was associated with hepatitis B virus infection, higher Edmondson-Steiner grade, and microvascular invasion. Panel-derived TMB values were evaluated descriptively, and exploratory survival analyses suggested potential differences between patient subgroups. Two candidate in-frame fusion signals, KIT-PDGFRA and ROS1-FBXL17, were detected in individual samples based on targeted DNA sequencing. Functional enrichment analyses indicated that mutated genes were mainly associated with cancer-related biological processes.</p><p><strong>Conclusions: </strong>This study provides an exploratory overview of somatic alterations detected by a targeted sequencing panel in a regional HCC cohort. Given the limitations inherent to panel-based analyses and the lack of independent validation, these findings should be interpreted cautiously and primarily serve as a reference for future large-scale and experimentally validated studies aimed at refining precision oncology strategies in HCC.</p>","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":"595-608"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic antitumor activity of CAR-NK cells combined with CAR-macrophages for pancreatic cancer treatment.","authors":"Dana Jung, Seo-Gyeong Jo, Eun-Yeung Gong, Ji-Hoon Kim, Kyungsoo Ha, Gabbine Wee, Seok Jae Huh, Young-Hee Jeoung, Seok-Ho Kim","doi":"10.1007/s13258-025-01733-9","DOIUrl":"10.1007/s13258-025-01733-9","url":null,"abstract":"<p><strong>Background: </strong>Adoptive cell therapies employing chimeric antigen receptors (CARs) have achieved remarkable success in hematologic malignancies, yet their efficacy in solid tumors remains limited due to the immunosuppressive tumor microenvironment (TME), which restricts immune cell infiltration and persistence. While CAR-NK cells provide potent cytotoxicity with a favorable safety profile, their tumor penetration is often suboptimal. In contrast, macrophages possess intrinsic tumor-homing and tissue-remodeling abilities that could help overcome these barriers.</p><p><strong>Objective: </strong>This study aimed to develop a combinatorial innate-cell immunotherapy integrating nanobody-based mesothelin (MSLN)-targeting CAR-macrophages (CAR-Ms) with CAR-NK cells to remodel the TME and enhance antitumor immunity in pancreatic cancer.</p><p><strong>Methods: </strong>Using a validated D3 nanobody CAR construct, CAR-Ms were generated from PMA-differentiated THP-1 monocytes and characterized for CAR expression, M1/M2 polarization, migration, phagocytosis, and cytokine secretion. CAR-NK cell migration and cytotoxicity were evaluated using conditioned media (CM) from CAR-M/tumor co-cultures. Synergistic antitumor activity was assessed in an orthotopic MSLN⁺ pancreatic ductal adenocarcinoma (PDAC) model.</p><p><strong>Results: </strong>D3-CAR-Ms exhibited robust CAR expression (> 90%) and sustained an M1-like phenotype (CD86⁺HLA-DR⁺CD204⁻) even after tumor engagement. Functionally, they displayed enhanced migration and infiltration into MSLN⁺ PANC-1 spheroids, along with increased phagocytic and tumoricidal activity. Upon antigen engagement, CAR-Ms secreted high levels of CXCL9, which promoted CAR-NK chemotaxis, degranulation, and cytotoxicity. Sequential administration of CAR-Ms and CAR-NK cells in vivo led to marked tumor regression and durable responses without systemic toxicity.</p><p><strong>Conclusion: </strong>Combinatorial nanobody-based CAR-M and CAR-NK therapy reprograms the TME and establishes a CXCL9-driven feed-forward loop between macrophages and NK cells, leading to synergistic innate immune activation and potent tumor control. This strategy provides a mechanistically grounded and translationally feasible framework for next-generation CAR-based immunotherapies targeting MSLN-expressing solid tumors.</p>","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":"523-532"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and expression analysis of the Hsf gene family in Rhododendron molle under heat stress.","authors":"Jieyu Peng, Shida Xu, Fanyu Zeng, Xingmin Geng, Jinliang Zhou","doi":"10.1007/s13258-025-01731-x","DOIUrl":"10.1007/s13258-025-01731-x","url":null,"abstract":"<p><strong>Background: </strong>Rhododendron possesses significant ornamental and economic value; however, its limited heat tolerance severely hinders its broader development and application. Heat shock transcription factors (Hsfs) are extensively involved in various abiotic stress responses and play essential roles in plant thermotolerance and other physiological processes.</p><p><strong>Objective: </strong>To identify Hsf genes in the genome of Rhododendron molle (R. molle) and investigate their regulatory mechanisms underlying heat tolerance in R. molle.</p><p><strong>Methods: </strong>The Hsf gene family was systematically identified using the genomic data of R. molle, and the expression levels of RmHsf genes under heat stress were analyzed through Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR).</p><p><strong>Results: </strong>The RmHsf gene family consists of 25 members distributed across 12 chromosomes. Phylogenetic analysis demonstrated that the RmHsf genes are classified into three subfamilies: A, B and C. Most genes within the same subfamily share similar conserved motifs and gene structures. The cis-acting elements in the promoter regions of RmHsf genes are associated with plant hormone signaling and stress response pathways. Collinearity analysis revealed that the expansion of the RmHsf gene family primarily occurred through segmental and tandem duplication events. RT-qPCR results showed that RmHsfA2, RmHsfA3, RmHsfA7a, and RmHsfA7b were significantly upregulated under heat stress, suggesting that they may serve as key genes in the heat stress response of R. molle. Among them, RmHsfA2 and RmHsfA3 were notably induced by exogenous ethylene.</p><p><strong>Conclusion: </strong>This study conducted a comprehensive genome-wide analysis of the Hsf gene family in R. molle, laying a solid foundation for functional validation of Hsf genes and the breeding of heat-tolerant cultivars.</p>","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":"509-522"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145951761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple machine learning algorithms construct cuproptosis genes and oxidative stress genes-related LncRNAs signature with prognostic and therapeutic relevance in ovarian cancer.","authors":"Ruyue Pan, Yan Yang, Qinghuo Kong, Xin Hu, Jie Yu, Jiaxu Chen","doi":"10.1007/s13258-025-01712-0","DOIUrl":"10.1007/s13258-025-01712-0","url":null,"abstract":"<p><strong>Background: </strong>Cuproptosis and oxidative stress (COS) are emerging regulators in cancer biology. However, their link to long non-coding RNAs (lncRNAs) and clinical outcomes in ovarian cancer remains unclear.</p><p><strong>Objective: </strong>This study aimed to construct and validate a prognostic signature based on COS-related lncRNAs to improve risk stratification and provide insights into therapeutic strategies for ovarian cancer.</p><p><strong>Methods: </strong>RNA-seq and clinical data from TCGA and GEO were analyzed to identify COS-related lncRNAs via WGCNA and Pearson correlation. Prognostic lncRNAs were screened using Cox regression and modeled using multiple machine learning algorithms. Immune profiles, mutation patterns, drug sensitivity, and experimental validation were performed.</p><p><strong>Results: </strong>A 12-lncRNA signature was established that stratified patients into high- and low-risk groups with significant survival differences (HR = 22.6145, p < 0.001). The model showed strong predictive performance (AUCs: 0.91/0.967/0.974) and was validated externally. High-risk patients exhibited greater mutation burden, altered immune pathways (interferon, TGF-β), and differential predicted sensitivity to agents like Axitinib and Pazopanib. RT-qPCR confirmed the expression patterns of 11 out of 12 lncRNAs.</p><p><strong>Conclusion: </strong>This study proposes a robust 12-lncRNA signature linking cuproptosis and oxidative stress with prognosis and therapy response in ovarian cancer, offering preliminary insights for personalized treatment guidance.</p>","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":"583-594"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145632054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UBL4A as a suppressor of intracerebral hemorrhage by binding to MAPK14 and promoting MAPK14 ubiquitination.","authors":"Yuyang Ou, Chen Chen, Ziyi Wang, Xi Zhu, Yaoxing Luo, Shulin Li, Dan Li","doi":"10.1007/s13258-025-01723-x","DOIUrl":"10.1007/s13258-025-01723-x","url":null,"abstract":"<p><strong>Background: </strong>Intracerebral hemorrhage (ICH) is a primary non-traumatic parenchymal hemorrhage of the brain with a high mortality and disability rate. Acupuncture has been proved to alleviate neurological deficits after ICH.</p><p><strong>Objective: </strong>Our previous study found that UBL4A is up-regulated in the ICH rat treated with acupuncture, and may be a potential molecular target for acupuncture treatment of ICH. However, the molecular mechanism behind UBL4A is unclear.</p><p><strong>Methods: </strong>UBL4A knockdown was conducted in ICH rats with acupuncture treatment to explore its role in the therapeutic effects of acupuncture on ICH. In vitro, the effects of UBL4A on heme-induced neuronal injury were evaluated using E18 rat embryonic primary cortical neurons. Proteomic experiments were used to verify the regulatory relationship between UBL4A and MAPK14.</p><p><strong>Results: </strong>UBL4A knockdown has opposite effects to the therapeutic effects of acupuncture on ICH, including increasing corner turn score, brain water content and the number of degenerated and apoptotic neuron in ICH rats. UBL4A knockdown increased the level of ROS, OPA1-S/L, p-MAPK14 and mitochondrial Drp1, and decreased mitochondrial membrane potential, ATP level and MFN2 level in rat brain tissue. In vitro, UBL4A overexpression reduced ROS level and mitochondrial membrane potential and inhibited the activation of the MAPK14/Drp1 signaling pathway. Co-IP verified the binding of UBL4A and MAPK14, and MAPK14 overexpression reversed the effect of UBL4A on hemin-induced neuronal apoptosis and ROS level.</p><p><strong>Conclusion: </strong>Our study confirms that UBL4A is involved in the therapeutic effect of acupuncture on ICH, and affects mitochondrial damage and oxidative stress through MAPK14 ubiquitination.</p>","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":"571-582"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146029306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative transcriptomics defines CD36 as a key regulator of immunometabolic signaling in acute myeloid leukemia.","authors":"Muhammad Sameer Ashaq, Qian Zhou, Yanxia Li, Meiqi Guo, Shujing Zhang, Lingling Wang, Zhuoran Li, Yi Wang, Yufeng Huang, Zhida Shi, Yuan Li, Baobing Zhao","doi":"10.1007/s13258-025-01728-6","DOIUrl":"10.1007/s13258-025-01728-6","url":null,"abstract":"<p><strong>Background: </strong>Acute myeloid leukemia (AML) is characterized by extensive immunometabolic rewiring that drives leukemic progression and fosters immune evasion.</p><p><strong>Objective: </strong>This study investigates regulatory role of CD36 as an immunometabolic mediator in AML pathogenesis.</p><p><strong>Methods: </strong>Integrated bulk transcriptomics, single-cell RNA sequencing, and in-vitro validation were performed. Macrophage co-localization was validated using colorectal cancer (CRC) spatial transcriptomics.</p><p><strong>Results: </strong>CD36 was identified as a central hub in preserved immunometabolic modules, enriched for TLR signaling, lipid metabolism, antigen-presenting pathways, and cytokine-cytokine receptor interactions. Drug sensitivity analysis revealed that high CD36 expression showed greater sensitivity to venetoclax and GSK626616AC. CD36 drives AML immune cycle and revealed a strong association with AML functional states, including inflammation, differentiation, apoptosis, invasion, quiescence, and hypoxia. Single-cell analysis indicated CD36 upregulation in monocyte and macrophage clusters, facilitating ligand-receptor communication with T cells, which emphasizes CD36's role in shaping immune microenvironment. Spatial transcriptomics analysis of colorectal cancer confirmed a significant CD36-CD68 colocalization in macrophages. CD36 also influenced monocyte to macrophage differentiation in AML cells. CD36 deficiency significantly reduces AML cells' proliferation and leads to G0/G1 phase expansion, accompanied by E2F4/E2F5/RB1 modulation. Hallmark enrichment analysis unveiled that CD36-high expression leukemic cells, CD36 immune signatures, and monocytes/macrophages showed enrichment in key immune and inflammatory pathways, including TNFα/NF-κB, IL6/JAK/STAT3, and IL2/STAT5 signaling, mTORC1 activation, interferon alpha and gamma responses, and reactive oxygen species pathways.</p><p><strong>Conclusion: </strong>Integration of transcriptomics and spatial validation revealed robust CD36-mediated immunometabolic signaling in AML, which further requires comprehensive in-vitro and in-vivo validation.</p>","PeriodicalId":12675,"journal":{"name":"Genes & genomics","volume":" ","pages":"487-507"},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145899972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}