Gastroenterology and Hepatology From Bed to Bench最新文献

{"title":"Investigating the effects of using Tecar therapy with biofeedback compared to biofeedback alone in the treatment of fecal incontinence in children aged 4 to 16 years: a randomized clinical trial study.","authors":"Azadeh Matlabi Lotfabadi, Fariba Ghaderi, Salman Nazary-Moghadam, Hamid Raza Kianifar, Parvin Sarbakhsh","doi":"10.22037/ghfbb.v18i1.3093","DOIUrl":"10.22037/ghfbb.v18i1.3093","url":null,"abstract":"<p><strong>Aim: </strong>Biofeedback has gained widespread recognition for its ability to facilitate and strengthen pelvic floor muscle function, making it a treatment of choice for these patients.</p><p><strong>Background: </strong>Fecal incontinence is a common issue among children, particularly those with chronic constipation, significantly affecting both the child's and their family's quality of life. Effective therapeutic interventions are essential to mitigate the symptoms and improve overall well-being. Physiotherapy for pediatric fecal incontinence focuses on strengthening, enhancing endurance, and improving coordination of the anal sphincter and pelvic floor muscles.</p><p><strong>Methods: </strong>This single-blind clinical trial examined the combined efficacy of Tecar therapy and biofeedback compared to biofeedback alone, with standard medical care serving as the control group. The study included 81 children diagnosed with fecal incontinence. Key outcomes evaluated were the severity of incontinence, severity of constipation, and frequency of incontinence episodes per week. These variables were evaluated before and after a six-week treatment period. Statistical analysis included repeated measures ANOVA for within-group comparisons and one-way ANOVA for between-group comparisons.</p><p><strong>Results: </strong>The results indicated significant improvements across all measured variables in the intervention groups compared to the control group. Notably, the combination of Tecar therapy and biofeedback outperformed biofeedback alone in certain aspects, such as lowering the severity of incontinence.</p><p><strong>Conclusion: </strong>The findings underscore physiotherapy as a non-invasive and effective first-line intervention for managing fecal incontinence and chronic constipation in pediatric populations. When feasible, the combination of Tecar therapy and biofeedback is recommended to achieve superior outcomes.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 1","pages":"82-90"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12301541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144741954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of viruses and molecular markers in the diagnosis and treatment of cancers and rare diseases: challenges and perspectives.","authors":"Amirhassan Rabbani, Nejat Mahdieh","doi":"10.22037/ghfbb.v18i3.3198","DOIUrl":"10.22037/ghfbb.v18i3.3198","url":null,"abstract":"","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 3","pages":"260-263"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12535776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic nutritional index and systemic inflammation index as predictors of cirrhosis severity: a cross-sectional study.","authors":"Behzad Hatami, Omid Ghanbarpour, Leila Pasharavesh, Amir Hassan Rabani, Toktam Alirezaei, Zahra Geshani, Sahar Kalami, Hosein Jafary","doi":"10.22037/ghfbb.v18i4.3195","DOIUrl":"10.22037/ghfbb.v18i4.3195","url":null,"abstract":"<p><strong>Aim: </strong>We assessed whether the Prognostic Nutritional Index (PNI) and the Systemic Inflammation Index (SII) are associated with cirrhosis severity and decompensation.</p><p><strong>Background: </strong>Cirrhosis severity is routinely staged with MELD and Child-Pugh scores, which only partially capture nutritional and inflammatory status.</p><p><strong>Methods: </strong>We conducted a cross-sectional study of 202 cirrhotic adults at Shahid Beheshti University hospitals (2018-2022). Demographic, clinical, and lab data were obtained from records. PNI (10 × albumin + 0.005 × lymphocytes) and SII (platelets × neutrophils ÷ lymphocytes) were calculated. Associations with MELD, Child-Pugh, and complications were analyzed (α = 0.05; SPSS).</p><p><strong>Results: </strong>Higher SII was associated with greater disease severity, including higher MELD (P = 0.040) and higher Child-Pugh class (overall P = 0.010), with SII levels higher in Child-Pugh class C than in class B (P = 0.001). PNI showed an inverse relationship with MELD, consistent with better nutritional status at lower severity. PNI was higher in patients without ascites (P = 0.020). Both indices differed in patients with hepatic encephalopathy versus those without (P = 0.010). Moreover, SII did not differ across variceal grades (P = 0.891), whereas PNI was lower in higher grades (overall P = 0.005).</p><p><strong>Conclusion: </strong>PNI and SII exhibit significant associations with established severity measures in cirrhosis. These indices may complement MELD and Child-Pugh by integrating nutritional and inflammatory information into routine assessment. Prospective, externally validated studies are warranted to determine predictive utility and clinical impact.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 4","pages":"418-425"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of complete and incomplete response to neoadjuvant therapy in rectum cancer patients.","authors":"Fatemeh TajikRostami, Majid Rezaei Tavirani, Elnaz Ataei, Mostafa Rezaei-Tavirani, Hamid Mohaghegh-Shalmani, Mohsen Norouzinia","doi":"10.22037/ghfbb.v18iSpecialIssue.3276","DOIUrl":"10.22037/ghfbb.v18iSpecialIssue.3276","url":null,"abstract":"<p><strong>Aim: </strong>Bioinformatic analysis of the differentially expressed genes (DEGs) of the two groups of patients is the main aim of this project.</p><p><strong>Background: </strong>Neoadjuvant therapy is a method to treatment disease before local surgery. This method has been administrated to treatment rectum cancer; complete and incomplete responses are observed in patients.</p><p><strong>Methods: </strong>The DEGs of gene expression profiles of patients with complete and incomplete response to treatment with neoadjuvant therapy were explored from recorded data in Gene Expression Omnibus (GEO) database and evaluated via protein-protein interaction (PPI) analysis and gene ontology assessment.</p><p><strong>Results: </strong>Among 606 significant DEGs, EGFR, JUN, CDK4, IGF1, and APP were pointed out as the key genes that are involved in response model to neoadjuvant therapy while \"GnRH signaling pathway\" and \"regulation of multicellular organism growth\" were introduced as the major involved groups of biological terms.</p><p><strong>Conclusion: </strong>It can be concluded that, there is genetic variation between rectum cancer patients and efficacy enhancement of treatment need considering precision medicine perspective.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 SI","pages":"31-39"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147940886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between colorectal cancer stem cells' expression of LGR5 and the location of colorectal cancer.","authors":"Imelda Rey, Rustam Effendi-Ys","doi":"10.22037/ghfbb.v18i4.3200","DOIUrl":"10.22037/ghfbb.v18i4.3200","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study is to assess the association between LGR5 expression, a cancer stem cell marker, and the location of colorectal cancer (CRC).</p><p><strong>Background: </strong>The incidence of chemotherapy-resistant CRC is increasing. The location of CRC is important in determining CRC progression. The area itself is associated with the presence of cancer stem cells.</p><p><strong>Methods: </strong>This cross-sectional study was conducted in Adam Malik Hospital and its sister hospitals. Inclusion criteria were patients who underwent colonoscopy, biopsy, or operation due to CRC, Indonesian, and willing to provide familial medical history regarding CRC and other malignancies up to third-degree relatives. CRC tissue was obtained from each subject as a specimen and underwent immunohistochemistry examination to determine LGR5 expression. Data analysis was performed by SPSS, version 26 (Chicago, 2022).</p><p><strong>Results: </strong>A total of 118 subjects were involved and divided into proximal (32 subjects), distal (43 subjects), and rectal (43 subjects) CRC. Mean age of subjects was 57.30 (SD 12.99) years with 69 (58.5%) subjects were males. The percentage of high-level LGR5 expression was found in subjects with proximal CRC (72%), followed by distal (44.1%) and rectal (39.5%) CRC. There was a significant association between LGR5 expression and the location of CRC (p=0.014). No relationship was observed between LGR5 expression with gender, age, body mass index, hemoglobin level, leukocyte count, platelet count, and histopathology grade of CRC.</p><p><strong>Conclusion: </strong>LGR5 expression is associated with CRC. Higher LGR5 expression is observed in proximal CRC.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 4","pages":"483-489"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147348260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can disulfiram modulate liver injury in CCl4-induced fibrosis in rats?","authors":"AbdolReza Rostami, Ravieh Golchoobian, Zohre Esmaeili, Mohammad Hossein Asghari, Nasim Hafezi, Zahra Babazadeh","doi":"10.22037/ghfbb.v18i4.3143","DOIUrl":"10.22037/ghfbb.v18i4.3143","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to evaluate the hepatoprotective and antifibrotic effects of DSF in a rat model of CCl₄-induced liver fibrosis.</p><p><strong>Background: </strong>Liver fibrosis is a chronic and potentially life-threatening condition characterized by fibroblast proliferation and excessive extracellular matrix deposition. Oxidative stress and inflammation are central mechanisms in its pathogenesis. Disulfiram (DSF), known for its antioxidant and potential antifibrotic properties, may attenuate liver injury.</p><p><strong>Methods: </strong>Thirty-five male Wistar rats were randomly divided into seven groups (n = 5 per group): Control, Oil, DSF 25 mg/kg, DSF 50 mg/kg, CCl₄, CCl₄ + DSF 25 mg/kg, and CCl₄ + DSF 50 mg/kg. After seven weeks of treatment, liver histopathology, serum liver enzymes (ALT, AST, ALP), and malondialdehyde (MDA) levels were assessed.</p><p><strong>Results: </strong>CCl₄ administration significantly increased serum ALT and AST levels compared with the Control group (p < 0.001). DSF at 25 mg/kg significantly reduced ALT (p = 0.0265), AST (p = 0.0334), and MDA (p < 0.001) compared with the CCl₄ group. Histopathological analysis revealed improved hepatocyte morphology in the low-dose DSF group (p = 0.0358). However, neither DSF dose produced a significant reduction in collagen deposition or vascular congestion, indicating no clear antifibrotic effect at these doses (>0.9999).</p><p><strong>Conclusion: </strong>Low-dose DSF demonstrated hepatoprotective effects through improvements in biochemical markers and hepatocyte morphology, but failed to significantly attenuate fibrosis. These findings suggest that lower DSF concentrations (< 25 mg/kg) may offer greater antifibrotic potential and should be further investigated in dose-response studies.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"18 4","pages":"426-437"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12920680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147347758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the effectiveness of citation count as a measure of methodological quality in esophagogastric surgery research: a comparative analysis with the MINORS score and levels of evidence.","authors":"Suhaib Js Ahmad, Ahmed R Ahmed, Ata Mohajer-Bastami, Sarah Moin, Benedict Sweetman, Sjaak Pouwels, Marion Head, Joseph Borucki, Anil Lala, Wah Yang, Christopher John Houlden, Tarek Garsaa, Aristomenis Exadaktylos","doi":"10.22037/ghfbb.v17i3.3005","DOIUrl":"10.22037/ghfbb.v17i3.3005","url":null,"abstract":"<p><strong>Aim: </strong>The primary objective was to assess the relationship between the citation number and the quality of the articles, as compared with the level of evidence and the MINORS score. This study's secondary objective was to characterize the 50 most cited articles in the field of oesophagectomy research.</p><p><strong>Background: </strong>There has been an increased need for an evaluation tool to indicate research quality. Available quality assessment tools include the Level of Evidence, the MINORS score, the Cochrane Risk of Bias 2.0 Tool, the Newcastle Ottawa Scale, CASP Appraisal Checklists, and Legend Evidence Evaluation tools.</p><p><strong>Methods: </strong>The Web of Science allowed evaluating and comparing articles on oesophagectomy research. The quality of the 50 most cited articles was assessed using the Oxford Centre level of evidence classification and the methodological index for non-randomized studies (MINORS).</p><p><strong>Results: </strong>Level of evidence II studies were cited more than level IV (P=0.008). There was a significant positive correlation between citation number and MINORS score (P=0.002). The median MINORS score was highest amongst level II studies, followed by levels III, IV, and I. The median MINORS score for level II evidence was significantly higher than for level IV (P=0.001). The study sample size is associated with higher levels of evidence but does not correlate with the citation number. Female authors contributed to 4 out of 50 articles. Recently published articles tended to be cited more frequently. More authors equated to more citations. Prospective studies are more likely to be cited.</p><p><strong>Conclusion: </strong>Citation analysis can be used as an indicator of quality when assessing articles. It should, however, be used with caution as highly cited work, famous authors, and journals are all more likely to be cited. Citation analysis should be used alongside other well-established tools.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 3","pages":"212-224"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142284283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translation, cultural adaptation, reliability, and validity of the Persian version of the Constipation Severity Instrument.","authors":"Zeinab Moradi, Farhad Azadi, Jalil Shoa Hasani, Mohsen Vahedi","doi":"10.22037/ghfbb.v17i2.2867","DOIUrl":"10.22037/ghfbb.v17i2.2867","url":null,"abstract":"<p><strong>Aim: </strong>The aim of this study was to translate and cross-culturally adapt the Constipation Severity Instrument (CSI) and assess its reliability and validity in the Iranian Persian language with chronic functional constipation.</p><p><strong>Background: </strong>Chronic functional constipation is a common complaint characterized by a range of symptoms. The use of a validated tool adapted to the culture is an important part of the treatment process.</p><p><strong>Methods: </strong>CSI was translated into Persian language according to Beaton's guidelines. One hundred and twenty-five patients with chronic functional constipation, according to ROME IV criteria, completed the questionnaires. Face validity was assessed in two qualitative and quantitative forms (impact score), internal consistency and test-retest reliability were assessed by Cronbach's alpha coefficient and intraclass correlation coefficient (ICC), respectively. Convergent validity was assessed by correlating the total scores of the CSI and the WCSS. The floor/ ceiling effects of the questionnaire were also assessed.</p><p><strong>Results: </strong>The impact score of all questions was greater than 1.5. The Cronbach's alpha coefficient for the total score was 0.90 and the ICC was 0.90. The total score of the CSI was significantly correlated with the total score of the WCSS (Spearman's p=0.74). No floor/ceiling effects were found.</p><p><strong>Conclusion: </strong>The Persian version of the CSI is a reliable and valid tool that can be used for psychometric evaluation. Clinicians can also benefit from this questionnaire when assessing treatment outcomes in Iranian patients.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 2","pages":"198-205"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of oral Midodrine in preventing hepatorenal syndrome in Child-Turcotte-Pugh class C cirrhotics: a pilot study.","authors":"Tariq Salim, Dadasaheb Maindad","doi":"10.22037/ghfbb.v17i4.3019","DOIUrl":"10.22037/ghfbb.v17i4.3019","url":null,"abstract":"<p><strong>Aim: </strong>The purpose of the study was to assess benefits of oral midodrine in the role of primary prevention of hepatorenal syndrome (HRS) in Child-Turcotte-Pugh Class C (CTP-C) cirrhotics.</p><p><strong>Background: </strong>The present non-randomized pilot study was designed for primary prevention of HRS as there is absence of an effective and definite treatment for this complication of cirrhosis to date other than liver transplant (LT).</p><p><strong>Methods: </strong>This study effectively involved 30 patients each enrolled in interventional and control arms suffering from liver cirrhosis CTP-C with normal renal function and having a mean arterial pressure (MAP) < 80 mmHg who were subjected to clinical examination and baseline blood investigations. The mean daily dosage of midodrine used across the study group was 16.75 mg.</p><p><strong>Results: </strong>At the end of 4 months of study, 11 individuals completed the study without attaining any endpoints from the control group while 23 accomplished it from the interventional arm. Nearly 50 % patients required a midodrine dose of 7.5 mg 8th hourly while the rest attained the targeted MAP with lower doses. By increasing MAP, the rate of HRS development during the study period (i.e. 4 months) was found to be significantly reduced in patients from interventional arm. The number needed to treat (NNT) observed in survival analysis to prevent one death was found to be 7.6.</p><p><strong>Conclusion: </strong>This study successfully established the role oral midodrine in primary prevention of HRS in cirrhotics at high risk. Midodrine was well tolerated with no significant adverse effects in patients under study.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 4","pages":"438-449"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting the NCAPD3 gene activates EGFR and ASNS as two pivotal contributors to gastric cancer progression.","authors":"Fatemeh Bandarian, Farideh Razi, Somayeh Jahani-Sharafat, Mohammad Rostami Nejad, Babak Arjmand, Masoumeh Farahani","doi":"10.22037/ghfbb.v17i4.3031","DOIUrl":"10.22037/ghfbb.v17i4.3031","url":null,"abstract":"<p><strong>Aim: </strong>This study was conducted to discover the effect of NCAPD3 knockdown on the gene expression profile of gastric cancer.</p><p><strong>Background: </strong>Gastric cancer, a potentially fatal disease, requires thorough evaluation for targeted interventions. Through the post-analysis of microarray data, it is crucial to further examine the impact of NCAPD3 (Non-SMC condensin II complex subunit D3) inhibition in gastric cancer, emphasizing the need for a more comprehensive analysis of this knockdown.</p><p><strong>Methods: </strong>The use of Cytoscape and its plug-ins for protein-protein interaction network analysis enables the identification of genes that significantly affect network stability. These hub-bottlenecks are regulated due to the NCAPD3 inhibition and some of them act as compensators in this condition. The hub-bottlenecks pathways identified by ClueGO indicate their relationships in underlying mechanisms of knockdown. These identified central differentially expressed genes could be considered eligible targets for therapeutic interventions. Some of them play compensative roles while others are regulated in NCAPD3 knockdown.</p><p><strong>Results: </strong>It can be concluded that some of the hub-bottlenecks contribute to compensation mechanisms including NPM1, PTEN, EGFR, HSPA5, and ASNS, while the other ones including HSPA4, DHX9, CAV1, MAP1LC3B, and SRSF1 are among the regulated genes.</p><p><strong>Conclusion: </strong>In particular, the up-regulation of EGFR and ASNS genes in the knockdown scenario could significantly impact and deteriorate cancer treatment outcomes after comprehensive validation studies.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 4","pages":"400-408"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}