Gastroenterology and Hepatology From Bed to Bench最新文献

{"title":"Effectiveness of human adipose tissue-derived mesenchymal stem cells expressing alpha-1 antitrypsin gene in liver fibrosis: a study in mice.","authors":"Sara Ali Hosseinzadeh, Abbas Sahebghadam Lotfi, Nahid Davoodian, Sareh Arjmand, Marjan Rangchi, Fatemeh Mashhadiabbas","doi":"10.22037/ghfbb.v17i2.2923","DOIUrl":"10.22037/ghfbb.v17i2.2923","url":null,"abstract":"<p><strong>Aim: </strong>The present study examined the protective potential of human adipose tissue-derived mesenchymal stem cells (hASCs) modified to overexpress alpha-1 antitrypsin (AAT), in a mouse model of the liver fibrosis.</p><p><strong>Background: </strong>For the treatment of end-stage liver diseases, cell therapy has emerged as a promising noninvasive alternative to liver transplantation. Mesenchymal stem cells (MSCs) are being evaluated due to their dual capabilities of promoting liver regeneration and modulating the pathogenic inflammation of the immune system.</p><p><strong>Methods: </strong>Liver fibrosis was induced in mice via the intraperitoneal injection of carbon tetrachloride (CCl4). MSCs were extracted from the human adipose tissue. After stemness confirmation, the cells were transduced with the lentiviruses containing the AAT gene, and then injected into the mice's tail vein. Fourteen days' post-transplantation, mice were sacrificed, and blood and tissue samples were collected for analysis. Important liver enzymes, including alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), albumin, and total bilirubin (TB), were measured. Histological studies were carried out using the hematoxylin and eosin (H&E), as well as Masson's trichrome (MT) staining.</p><p><strong>Results: </strong>Compared to hASCs, treatment with AAT-hASCs resulted in greater reductions in ALT, AST, ALP, and TB, as well as normalized albumin levels. AAT-hASCs promoted enhanced liver regeneration histologically, likely attributable to anti-inflammatory and anti-proteolytic properties of AAT.</p><p><strong>Conclusion: </strong>These findings indicate AAT-engineered hASCs as a promising cell-gene therapy candidate for further study in liver cirrhosis models.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 2","pages":"151-160"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence and bioinformatics: a journey from traditional techniques to smart approaches.","authors":"Hamid Jamialahmadi, Ghazaleh Khalili-Tanha, Elham Nazari, Mostafa Rezaei-Tavirani","doi":"10.22037/ghfbb.v17i3.2977","DOIUrl":"10.22037/ghfbb.v17i3.2977","url":null,"abstract":"<p><p>The incorporation of AI models into bioinformatics has brought about a revolutionary era in the analysis and interpretation of biological data. This mini-review offers a succinct overview of the indispensable role AI plays in the convergence of computational techniques and biological research. The search strategy followed PRISMA guidelines, encompassing databases such as PubMed, Embase, and Google Scholar to include studies published between 2018 and 2024, utilizing specific keywords. We explored the diverse applications of AI methodologies, including machine learning (ML), deep learning (DL), and natural language processing (NLP), across various domains of bioinformatics. These domains encompass genome sequencing, protein structure prediction, drug discovery, systems biology, personalized medicine, imaging, signal processing, and text mining. AI algorithms have exhibited remarkable efficacy in tackling intricate biological challenges, spanning from genome sequencing to protein structure prediction, and from drug discovery to personalized medicine. In conclusion, this study scrutinizes the evolving landscape of AI-driven tools and algorithms, emphasizing their pivotal role in expediting research, facilitating data interpretation, and catalyzing innovations in biomedical sciences.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 3","pages":"241-252"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142284250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of toxin gene expression levels and molecular typing of <i>Clostridioides difficile</i> strains isolated from patients with diarrhea.","authors":"Leili Shokoohizadeh, Mahnaz Moomivand, Abbas Yadegar, Masoumeh Azimirad, Seyyed Hamid Hashemi, Mohammad Yousef Alikhani","doi":"10.22037/ghfbb.v17i3.2982","DOIUrl":"10.22037/ghfbb.v17i3.2982","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to evaluate the expression of <i>tcd</i>A, <i>tcd</i>B, and binary toxin genes (<i>cdt</i>A and <i>cdt</i>B) by Real-Time PCR and molecular typing of <i>Clostridioides difficile</i> isolated from patient diarrhea samples from Hamadan Hospitals, west of Iran.</p><p><strong>Background: </strong>The concentration of <i>C. difficile</i> toxins (CDTs) is associated with the severity of the disease and the mortality rate. Measuring CDT levels could provide a reliable and objective means of determining the severity of <i>C. difficile</i> infection (CDI).</p><p><strong>Methods: </strong>From November 2018 to September 2019, 130 diarrhea samples were collected from hospitalized patients in three hospitals in Hamadan. <i>C. difficle</i> isolates were detected by culture and PCR. The presence of the genes encoding the toxin was identified by PCR, whereas the measurement of toxin expression was conducted using a relative Real-Time PCR technique. Genetic linkage of the isolates was also assessed by Ribotyping and Repetitive Extragenic Palindromic (rep-PCR) methods.</p><p><strong>Results: </strong>Among 130 diarrhea samples, 16 (12.3%) were positive for <i>C. difficile</i>. Genes encoding <i>cdt</i>A and <i>tcd</i>B were detected in all isolates, and 8 (50%) and 6 (37.5%) isolates were positive for the <i>cdt</i>A and <i>cdt</i>B genes. Real-time PCR results showed different expression levels of the toxin genes. A significant increase in the expression of the <i>tcd</i>A gene was observed compared with the control strain (P<0.05). Besides, more expression of <i>cdt</i>A gene was observed in the strains compared with <i>cdt</i>B gene. Ribotyping and rep-PCR results showed high genetic diversity of <i>C. difficile</i> among hospitals investigated.</p><p><strong>Conclusion: </strong>We encountered toxigenic <i>C. difficile</i> strains with various toxin expression levels, ribotypes, and rep types based on the findings of this study. This indicated that various clones from various sources circulate in the hospitals and among patients.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 3","pages":"304-312"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142284252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stage analysis of pancreatic ductal adenocarcinoma via network analysis.","authors":"Ayad Bahadorimonfared, Masoumeh Farahani, Mostafa Rezaei Tavirani, Zahra Razzaghi, Babak Arjmand, Mitra Rezaei, Abdolrahim Nikzamir, Mohammad Javad Ehsani Ardakani, Vahid Mansouri","doi":"10.22037/ghfbb.v17i3.2887","DOIUrl":"10.22037/ghfbb.v17i3.2887","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to introduce a biomarker panel to detect pancreatic ductal adenocarcinoma (PDAC) in the early stage, and also differentiate of stages from each other.</p><p><strong>Background: </strong>PDAC is a lethal cancer with poor prognosis and overall survival.</p><p><strong>Methods: </strong>Gene expression profiles of PDAC patients were extracted from the Gene Expression Omnibus (GEO) database. The genes that were significantly differentially expressed (DEGs) for Stages I, II, and III in comparison to the healthy controls were identified. The determined DEGs were assessed via protein-protein interaction (PPI) network analysis, and the hub-bottleneck nodes of analyzed networks were introduced.</p><p><strong>Results: </strong>A number of 140, 874, and 1519 significant DEGs were evaluated via PPI network analysis. A biomarker panel including ALB, CTNNB1, COL1A1, POSTN, LUM, and ANXA2 is presented as a biomarker panel to detect PDAC in the early stage. Two biomarker panels are suggested to recognize other stages of illness.</p><p><strong>Conclusion: </strong>It can be concluded that ALB, CTNNB1, COL1A1, POSTN, LUM, and ANXA2 and also FN1, HSP90AA1, LOX, ANXA5, SERPINE1, and WWP2 beside GAPDH, AKT1, EGF, CASP3 are suitable sets of gene to separate stages of PDAC.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 3","pages":"297-3030"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11413388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142284287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypolipidemic and hepatoprotective effects of corn silk extract in nicotine-administered male mice.","authors":"Anahita Mohammadi, Ali Akbar Oroojan, Mehdi Hashemi, Seyedeh Mahsa Poormoosavi, Mojtaba Dolatshahi, Fatemeh Golshokouh","doi":"10.22037/ghfbb.v17i1.2806","DOIUrl":"10.22037/ghfbb.v17i1.2806","url":null,"abstract":"<p><strong>Aim: </strong>This study is done to investigate the hypolipidemic and hepatoprotective effects of corn silk extract in nicotine-administered male mice.</p><p><strong>Background: </strong>Nicotine can induce pathophysiological effects in the liver tissue through oxidative stress and damage cells. Corn silk can improve liver function with its antioxidant effects.</p><p><strong>Methods: </strong>In this experimental study, 30 male NMRI mice (25-30 gr) were divided into 5 groups: controls, sham, nicotine 2.5 mg/kg, nicotine+aqueous extract of corn silk 400 mg/kg, and nicotine+methanolic extract of corn silk 400 mg/kg for 1 month. One day after the last nicotine and extracts consumption, the serum samples were performed for biochemical measurement, and the supernatant of the homogenized liver was administered for antioxidant variables assessment.</p><p><strong>Results: </strong>There was no significant difference in the body weight of different groups. Liver weight and GSH decreased in the nicotine group compared to the control group (P<0.05). Triglycerides, total cholesterol, HDL-C, LDL-C, liver enzymes, and MDA increased in the nicotine group compared to the control group (P<0.05). Also, the expansion of sinusoids, the presence of inflammatory cells, and necrosis of liver cells were observed in the nicotine group compared to the control group. Using aqueous and methanolic extracts of corn silk in mice receiving nicotine led to the improvement of the mentioned variables (P<0.05).</p><p><strong>Conclusion: </strong>The results of this study showed that the use of nicotine can lead to the induction of hepatotoxicity. The use of aqueous and methanolic extracts of corn silk improved them through its antioxidant activity.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 1","pages":"64-73"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11080695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possible association between polyomaviruses and gastrointestinal complications: a narrative review.","authors":"Piruz Shadbash, Seyed Masoud Hosseini, Shahrzad Shoraka, Amir Ghaemi, Mehrdad Haghazali, Seyed Reza Mohebbi","doi":"10.22037/ghfbb.v17i2.2796","DOIUrl":"10.22037/ghfbb.v17i2.2796","url":null,"abstract":"<p><p>Polyomaviruses are a group of small, double-stranded DNA viruses that are known to be associated with the development of certain human diseases, but there is evidence that these viruses might be associated with gastrointestinal (GI) cancers. Several polyomaviruses have been identified, such as JC polyomavirus (JCPyV), BK polyomavirus (BKPyV) and recently Merkel cell polyomavirus (MCPyV). Although the direct effects of polyomaviruses on transformation of human cells and cancer development are not clearly recognized, their association with certain human diseases including GI cancers has been proposed through several molecular and epidemiological studies. For example, JCPyV and BKPyV have been linked to colorectal cancer, as there is growing evidence of finding viral genomes in cancerous tissues. Nevertheless, the major role of JCPyV, BKPyV and MCPyV in colorectal cancer progression is still under extensive investigation, and further surveys is required to establish a conclusive cause-and-effect relationship. Understanding the role of these viruses in cancer development has significant implications for diagnosis, treatment, and prevention strategies. It seems that proving a causal link between polyomaviruses and GI cancers might provide a novel path for targeted therapies or design and development of specific therapeutic vaccines. In addition, performing research on the possible link can provide insights into the underlying molecular mechanisms of carcinogenesis, potentially leading to the identification of novel biomarkers. This review focuses on polyomaviruses, in particular a recently discovered polyomavirus, MCPyV, and their possible link with human gastrointestinal disorders.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 2","pages":"121-131"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pregnancy outcome in patients with non-alcoholic fatty liver disease: a prospective cohort study.","authors":"Razieh Mohammad Jafari, Elham Karimi Moghaddam, Azar Ahmadzadeh, Samaneh Bahrami, Pezhman Alavinejad, Samira Manouchehri Zanjani","doi":"10.22037/ghfbb.v17i2.2896","DOIUrl":"10.22037/ghfbb.v17i2.2896","url":null,"abstract":"<p><strong>Aim: </strong>The purpose of this investigation was to examine the potential association between non-alcoholic fatty liver disease (NAFLD) and adverse maternal and perinatal outcomes during pregnancy.</p><p><strong>Background: </strong>Gaining insights into the effect of NAFLD on pregnancy outcomes is essential to ensure the health and well-being of mothers and infants.</p><p><strong>Methods: </strong>This prospective cohort study was conducted at Imam Khomeini and Razi hospitals of Ahvaz City in 2022. Totally, 180 pregnant women in the NAFLD group to 180 in the control group. In this study, a researcher-made checklist was used to collect the background information, medical history, and lab data during their initial visit using. Follow-up continued until one week after delivery, with pregnancy outcomes assessed. Statistical analysis used student's t-test and the Chi-Square test for group comparisons.</p><p><strong>Results: </strong>Significant differences were observed between the NAFLD, and control groups in terms of age (P=0.003), BMI (P=0.016), ALT and AST measures (P<0.001), and hypertensive complications (P=0.044). The NAFLD group had higher rates of gestational diabetes (P<0.001) and gestational hypertension (P=0.003). However, no significant differences were found in gestational age at delivery, early postpartum hemorrhage rates, birth weight, and neonatal Apgar scores (P>0.05).</p><p><strong>Conclusion: </strong>The pregnant women with NAFLD may be at risk for various complications during pregnancy, including a higher prevalence of gestational diabetes, elevated liver enzymes, and higher blood pressure compared to healthy pregnant women. However, the research failed to identify any statistically significant disparities between infants born to mothers with NAFLD and those delivered to healthy mothers in relation to birth weight, Apgar scores, or neonatal mortality.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 2","pages":"180-186"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare differential diagnosis of inflammatory bowel disease in a young male patient; a challenging case report.","authors":"Morteza Valaei, Azita Ganji, Marieh Alizadeh","doi":"10.22037/ghfbb.v17i2.2898","DOIUrl":"10.22037/ghfbb.v17i2.2898","url":null,"abstract":"<p><strong>Background: </strong>Chronic granulomatous disease (CGD) is a rare disorder normally diagnosed in infancy.</p><p><strong>Case presentation: </strong>A 27-year-old man admitted with non-specific symptoms of CGD first underwent endoscopy, and colonoscopy procedures as primary evaluation of clinical presentation. Eighteen months after the first admission, he was referred to the emergency department for hematemesis, and critical situations, such as a severe anemic with Hgb= 2.6 mg/dl. As a result of this specific clinical presentation, urgent emergency treatment was performed, and endoscopic examination revealed ulcers and abnormalities in the duodenal bulb and jejunum. Other imaging procedures, such as sonography, and abdominal CT scans, showed splenomegaly. He underwent splenectomy, and after that, endoscopic treatment with balloon TTS dilation was scheduled, but this procedure failed. So, we decided to do a gastro-jujenostomy that alleviated the clinical symptoms. After nine months, he was referred to GOO, and endoscopic evaluation showed giant ulceration with severe stricture in the duodenum, and a polyp in the jejunostomy. Finally, Based on clinical presentation and pathologic evidence of biopsies, the patient approached CGD as the final diagnosis.</p><p><strong>Conclusion: </strong>Step-by-step, rule out of different highly suspicious diseases may result in a definite CGD diagnosis, and rapid management of these patients may increase the chance of survival.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 2","pages":"206-211"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute-on-chronic liver failure; prevalence, causes, predisposing factors, and outcome.","authors":"Kamran Bagheri Lankarani, Zahra Ghanbarinasab, Ramin Niknam","doi":"10.22037/ghfbb.v17i2.2888","DOIUrl":"10.22037/ghfbb.v17i2.2888","url":null,"abstract":"<p><strong>Aim: </strong>Until now, there has been disagreement regarding the prevalence, causes, predisposing factors, and outcome of ACLF (Acute-on-chronic liver failure). As a result, we have undertaken this research study.</p><p><strong>Background: </strong>ACLF is a complex syndrome with a poor prognosis.</p><p><strong>Methods: </strong>In this cross-sectional study, we evaluated the prevalence, causes, predisposing factors, and outcomes of adult cirrhotic patients with ACLF and acute decompensation (AD). ACLF was defined based on the criteria established by APASL (Asian Pacific Association for the Study of the Liver). The severity of organ failure was assessed using both EASL-CLIF (European Association for the Study of the Liver- Chronic Liver Failure) and NACSELD (North American Consortium for the Study of End-Stage Liver Disease) scores. To investigate the impact of different independent variables on mortality, survival analysis methods were used.</p><p><strong>Results: </strong>A total of 156 patients' data were analyzed in this study. The mean age of patients with ACLF (56.62±16.19 years) was significantly lower compared to the AD group (62.30±14.28 years). Nonalcoholic steatohepatitis and infection were the most common causes and predisposing factors in both AD and ACLF groups, respectively, but the difference between the two groups was not statistically significant. The most common organ failures observed were hepatic encephalopathy and respiratory failure. The probability of death at any given time for was significantly higher in ACLF patients than in the AD group (log rank test; P<0.001). The results of Cox regression analysis revealed that low blood pressure (HR 0.97; 95% CI 0.96-0.99; P<0.001) and decreased blood pH (HR 0.53; 95% CI 0.28-0.99; P=0.04) were significant risk factors associated with increased mortality.</p><p><strong>Conclusion: </strong>ACLF patients had a lower average age and higher mortality rates compared to AD. Nonalcoholic steatohepatitis was found to be the most common underlying disease in ACLF patients, while infections were identified as the predominant predisposing factor. All cases of mortality in the ACLF group were categorized as grade 3 and 4 based on the EASL-CLIF severity score. Hemodynamic instability and metabolic acidosis emerged as the most significant risk factors associated with increased mortality.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 2","pages":"161-170"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current status of intestinal parasitic infections and associated risk factors in rural population of Guilan province, northern Iran: trichostrongyliasis is the most prevalent helminthic infection.","authors":"Keyhan Ashrafi, Maryam Shekari, Meysam Sharifdini, Mohammad Reza Mahmoudi, Zahra Atrkar Roshan, Behnaz Rahmati, Sayed Mahmoud Rezvani, Arya Omidvar, Hamed Mirjalali","doi":"10.22037/ghfbb.v17i2.2893","DOIUrl":"10.22037/ghfbb.v17i2.2893","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to determine the distribution of enteric parasitic infections and related risk factors among rural communities of Guilan province, Northern Iran, and to compare the results with the situation in the past.</p><p><strong>Background: </strong>Intestinal parasitic infections are still considered as a major public health concern, particularly in human communities with poor economy and sanitation.</p><p><strong>Methods: </strong>This cross-sectional study was performed in rural areas of Masal and Shanderman district from February to December 2020. A total of 917 stool samples were collected and examined for presence of intestinal helminthes and protozoa using direct, formalin-ether and Kato-Katz techniques.</p><p><strong>Results: </strong>A total of 156 (17%) out of 917 examined individuals were infected with intestinal parasites. The overall prevalence of protozoa, helminths and mixed infections were 11.8% (108/917), 4.5% (41/917) and 0.8% (7/917), respectively. Blastocystis was the most prevalent intestinal protozoa (9.6%) followed by Giardia lamblia (1.9%), Endolimax nana (1.1%), E. coli (0.8%) and Entamoeba hartmani (0.1%). The highest prevalence of intestinal helminths belonged to Trichostongylus spp. (3.5%) and Strongyloides stercoralis (1.3%). Statistical analysis showed significant association between giardiasis and sex (P<0.03). On the other hand, prevalence of enteric helminths was influenced by close contact with livestock, keeping herbivorous animals at home, job, education, and consumption of uncooked vegetables (P<0.05).</p><p><strong>Conclusion: </strong>The findings indicate a decreasing trend in the prevalence of intestinal parasitic infections in Guilan province in comparison to the past few decades. Hookworm infections, which was very prevalent in the area, are now rare, while trichostrongylosis showed a high prevalence in rural residents of the study area.</p>","PeriodicalId":12636,"journal":{"name":"Gastroenterology and Hepatology From Bed to Bench","volume":"17 2","pages":"187-197"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}