Gastroenterology Research and Practice最新文献

{"title":"Improving the Accuracy of Endoscopic Measurements of the Size of Gastrointestinal Lesions by a Guidewire Scale.","authors":"Nanthawat Talalak, Saritphat Orrapin, Sittichock Wattanarochanaporn, Teerawat Sotananan, Thanakorn Likidkarnchanakornkij, Prasit Mahawongkajit","doi":"10.1155/grp/3466541","DOIUrl":"10.1155/grp/3466541","url":null,"abstract":"<p><strong>Objectives: </strong>Using an endoscope to accurately measure the size of a lesion is crucial for assessing the healing process and determining the treatment strategies for the stage of the cancer. Visual estimation of the lesion size is commonly used, but this is often inaccurate. To address this issue, a guidewire scale was developed to measure lesion size and improve accuracy at a practical level.</p><p><strong>Methods: </strong>Four endoscopists estimated the size of artificial lesions in the gastric model by visual comparison with the guidewire measurements. The endoscopists used a guidewire scale to determine the size of the lesion and rated their satisfaction after the procedure using a numeral rating scale (NRS).</p><p><strong>Results: </strong>Fifty-two patients were recruited and assessed for the size of their lesions using a guidewire scale. Overall, the endoscopists reported good satisfaction levels (NRS ≥ 8), with satisfaction significantly higher in the dyspepsia group (<i>p</i> = 0.03). The mean lesion size was 10.7 ± 8.9 mm, with smaller lesions (≤ 1 cm) resulting in higher satisfaction levels. On average, it took 39.4 ± 13.6 s to measure the lesion, with a procedure time of ≤ 30 s resulting in very high satisfaction levels.</p><p><strong>Conclusions: </strong>The accuracy of endoscopic measurements was improved using the guidewire scale, allowing clinical studies to safely and easily estimate lesion sizes.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2026 ","pages":"3466541"},"PeriodicalIF":1.6,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12907985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146212999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Integrated Lecture-, Case-, and Problem-Based Learning on Critical Thinking and Clinical Competence in Gastrointestinal Surgery Trainees: A Randomized Controlled Trial.","authors":"Jin Tang, Jing Yang, Junsong Yang, Shoujiang Wei","doi":"10.1155/grp/8457575","DOIUrl":"10.1155/grp/8457575","url":null,"abstract":"<p><strong>Background: </strong>Enhancing clinical thinking and competence is central to gastrointestinal surgical training. This study is aimed at evaluating the effectiveness of an integrated instructional model combining lecture-based learning (LBL), problem-based learning (PBL), and case-based learning (CBL) in gastrointestinal surgical training.</p><p><strong>Methods: </strong>This prospective randomized controlled trial was conducted at the Affiliated Hospital of North Sichuan Medical College, China, from September to November 2024. A total of 120 gastrointestinal surgical trainees, including final-year medical interns and first-year surgical residents, were randomly assigned (1:1:1) to the LBL, PBL + CBL, or integrated LBL + PBL + CBL groups using a computer-generated sequence, with allocation concealed by an independent assistant. After completing an 8-week standardized curriculum delivered by the same teaching team, the following outcomes were assessed: critical thinking ability using the Chinese version of the California critical thinking disposition inventory (CTDI-CV); theoretical knowledge through written and oral examinations; learning efficiency (study time and task completion rate) and satisfaction; and clinical competence assessed via the miniclinical evaluation exercise (Mini-CEX). Data were analyzed using IBM SPSS Statistics with one-way analysis of variance, and statistical significance was set at <i>p</i> < 0.05.</p><p><strong>Results: </strong>The integrated LBL + PBL + CBL groups achieved the highest CTDI-CV total scores, theoretical knowledge performance, and Mini-CEX scores compared with the other groups (<i>p</i> < 0.05). Despite reporting the shortest weekly study time, the integrated group attained the highest task completion rate and learner satisfaction score (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>The integrated LBL + PBL + CBL approach enhances efficiency and is well-suited for advancing clinical competence in surgical training.</p><p><strong>Trial registration: </strong>Chinese Clinical Trial Registry (ChiCTR2000041569).</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2026 ","pages":"8457575"},"PeriodicalIF":1.6,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12887692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Characteristics of Late-Onset (≥ 65 Years) Ulcerative Colitis: A Single-Center Retrospective Study.","authors":"Ayumi Ito, Miki Koroku, Syun Murasugi, Maria Yonezawa, Teppei Oomori, Shinichi Nakamura, Katsutoshi Tokushige, Yousuke Nakai","doi":"10.1155/grp/8860301","DOIUrl":"10.1155/grp/8860301","url":null,"abstract":"<p><strong>Background: </strong>Late-onset ulcerative colitis (UC) has become prevalent in recent years. The differences between late-onset, middle aged-onset, and early-onset UC have not been fully elucidated.</p><p><strong>Methods: </strong>The clinical characteristics, steroid side effects, remission rates, and surgical rates of UC patients were retrospectively studied. Patients were allocated to three groups according to age at diagnosis: late-onset group (≥ 65 years), middle aged-onset (50-64 years), and early-onset group (≤ 49 years).</p><p><strong>Results: </strong>Clinical characteristics such as admission age, duration of disease, history of cancer, days to diagnosis, clinical activity on admission, hemoglobin level on admission, and steroid use were significantly different between the three groups. The side effects of steroids were significantly more common in the late-onset group. The remission induction rates differed significantly between the three groups: 67.7%, 71.7%, and 83.6% in the late-onset, middle aged-onset, and early-onset groups. Surgery rates were significantly higher in the late-onset group: Surgery rates in the late-onset, middle aged-onset, and early-onset groups were 14.2%, 3.4%, and 3.4% at 12 months of diagnosis, and 23.8%, 10.3%, and 4.2% at 24 months of diagnosis.</p><p><strong>Conclusions: </strong>In conclusion, remission rates were low and surgical rates were higher with increasing age of onset. In addition, steroid-related side effects increased with age. It was possible that late-onset UC may have a different pathogenesis and be resistant to treatment.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2026 ","pages":"8860301"},"PeriodicalIF":1.6,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12836296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146092998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compound Sophorae Decoction Alleviates Ferroptosis in Colitis Rats via Activating Keap1/Nrf2/GPX4 Signaling Pathway.","authors":"Jingbo Wang, Qianyun Chen, Si Chu, Feng Zhu, Lijuan Zhang, Zaifeng Yi, Jingjing Li, Desheng Hu, Heng Fan, Ting Yu","doi":"10.1155/grp/6298090","DOIUrl":"10.1155/grp/6298090","url":null,"abstract":"<p><strong>Background: </strong>Compound sophorae decoction (CSD) has been extensively applied in clinic for the treatment of ulcerative colitis (UC). However, the effect and precise therapeutic mechanism have not been fully clarified. In this study, we systematically explored the protective efficacy and the underlying molecular mechanisms of CSD against UC.</p><p><strong>Methods: </strong>The main constituents of CSD were analyzed by HPLC-MS/MS and TLC. H₂O₂-treated Caco-2 cells were employed to investigate the impact of CSD on ferroptosis and its underlying mechanism. The impacts of CSD on inflammation, oxidative stress, and ferroptosis <i>in vitro</i> were evaluated by ELISA, biochemical detection, or fluorescence probe. The UC model was established in rats by administering 5% DSS in the drinking water. The effects and safety of CSD on DSS-induced colitis were evaluated through daily body weight, DAI, colon length, and HE staining. In addition, cytokines (IL-1<i>β</i>, IL-6, TNF-<i>α</i>, and TGF-<i>β</i>) and ferroptosis-associated parameters (iNOS and PTGS2) were detected by ELISA. Antioxidant and oxidant enzyme activities (SOD, GSH, MDA, and NO) and lipid ROS in serum and colon tissue were measured by biochemical kit. Ferroptosis was determined by analysis of ferroptosis-associated proteins (Keap1, Nrf2, GPX4, and SLC7A11).</p><p><strong>Results: </strong>Then, 10 main active components were identified in CSD. CSD significantly attenuated DSS-induced intestinal injury and inflammation. Moreover, CSD notably decreased oxidative stress and lipid peroxidation. Mechanistically, CSD suppressed ferroptosis in DSS-induced UC and upregulated GPX4 and SLC7A11 expression through the activation of Nrf2 signaling in DSS-induced rats.</p><p><strong>Conclusions: </strong>Collectively, this study demonstrated that CSD ameliorates ferroptosis in DSS-induced UC rats, with its protective effects attributed to the activation of the Keap1/Nrf2/GPX4 signaling pathway.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"6298090"},"PeriodicalIF":1.6,"publicationDate":"2025-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12752875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145877681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes in Patients Undergoing Transoral Incisionless Fundoplication With Ineffective Esophageal Motility.","authors":"Dhruvanshu Patel, Zarian Prenatt, Emilie S Kim, Parampreet Kaur, Amit Sohagia, Vhada S Sharma, Chandani Patel, Madhav Changela, Krutarth Shukla, Ronak Modi","doi":"10.1155/grp/5054381","DOIUrl":"10.1155/grp/5054381","url":null,"abstract":"<p><strong>Background: </strong>Transoral incisionless fundoplication (TIF) presents a minimally invasive, endoscopic treatment for the management of gastroesophageal reflux disease (GERD), known for its efficacy and safety in patients with normal esophageal motility. However, the use of TIF in ineffective esophageal motility (IEM) remains largely unexplored. This study examined the outcomes of TIF in IEM patients.</p><p><strong>Methods: </strong>Retrospective data from 164 patients, including 29 with IEM and refractory GERD, were analyzed to obtain assessments of disease-specific quality of life through the Gastroesophageal Reflux Disease Health-Related Quality of Life Questionnaire (GERD-HRQL) and proton pump inhibitor usage.</p><p><strong>Results: </strong>Statistically significant improvements were seen in total, heart burn, and regurgitation GERD-HRQL scores (<i>p</i> < 0.001, <i>p</i> < 0.001, <i>p</i> < 0.001) and reduction in PPI use (0 < 0.001) after TIF, with no significant difference in dysphagia risk (<i>p</i> < 0.2).</p><p><strong>Conclusion: </strong>This study underscores durable improvements that TIF can provide in quality of life in patients with both GERD and IEM, without compromising the increased risk of dysphagia. Although some patients resume antisecretory medications within 3-6 months, most stopped taking PPI after the procedure.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"5054381"},"PeriodicalIF":1.6,"publicationDate":"2025-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12752835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145878020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histologic Reappraisal and Evaluation of MLH1 Protein Expression in Sessile Serrated Lesions of the Proximal Colon.","authors":"Priscilla de Sene Portel Oliveira, Miriam Aparecida da Silva Trevisan, Rita Barbosa de Carvalho, Rita de Cássia Perina Martins, João José Fagundes, Claudio Saddy Rodrigues Coy","doi":"10.1155/grp/5970839","DOIUrl":"10.1155/grp/5970839","url":null,"abstract":"<p><strong>Introduction: </strong>Changes in nomenclature and in the criteria for histological diagnosis of the serrated lesions have occurred over the years. Some of these lesions, the sessile serrated lesions (SSLs), progress to adenocarcinoma via suppression of the <i>MLH1</i> gene, an important carcinogenic pathway.</p><p><strong>Objective: </strong>Evaluate the frequency of reclassification in histological diagnosis from hyperplastic polyps (HPs) to SSL after reappraisal using the 2019 World Health Organization (WHO) classification, to determine the occurrence of previously undiagnosed dysplasia and to study the expression of the <i>MLH1</i> protein in SSLs and SSLDs.</p><p><strong>Methodology: </strong>Lesions with histological diagnosis of HP, SSL, and SSLD resected by colonoscopies performed between 2005 and 2015 located in the proximal colon were studied. All HPs were submitted for histological review by two pathologists (Examiners 1 and 2), and a third experienced pathologist (Examiner 3) made the final decision when the other examiners did not agree. Interobserver agreement was analyzed. <i>MLH1</i> protein expression was assessed by immunohistochemistry in lesions diagnosed as SSL and SSLD before and after reappraisal. These lesions were reviewed again for missed dysplasia.</p><p><strong>Results: </strong>A total of 308 lesions were assessed being 287 with the initial diagnosis of HP and 21 SSL. Thirty-eight (13.3%) lesions with an initial diagnosis of HP had their diagnosis reclassified to SSL. No dysplasia was found. There was a moderate agreement (Kappa 0.52) between Examiners 1 and 2 regarding the diagnosis of SSL. Between Examiners 1 and 3, there was no agreement (Kappa -0.19), and between Examiners 3 and 2, the agreement was poor (Kappa 0.13). All 38 lesions analyzed by immunohistochemistry had <i>MLH1</i> expression.</p><p><strong>Conclusion: </strong>Changes in diagnosis from HP to SSL occurred in 13.3%. No dysplasia or lack of MLH1 expression was observed.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"5970839"},"PeriodicalIF":1.6,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12752821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145877904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Histomorphological Characteristics and Pathological Types of Hyperproliferation of Gastric Surface Epithelial Cells\".","authors":"","doi":"10.1155/grp/9831902","DOIUrl":"10.1155/grp/9831902","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1155/2021/8828326.].</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"9831902"},"PeriodicalIF":1.4,"publicationDate":"2025-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12752818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145877887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-Related Differences in Clinical Presentation of Autoimmune Gastritis: A Retrospective Comparative Study.","authors":"Ren Song, Qi Chen, Xiao-Ling Wu, Ming Luo, Gang Hu","doi":"10.1155/grp/4250339","DOIUrl":"10.1155/grp/4250339","url":null,"abstract":"<p><strong>Purpose: </strong>This study was aimed at investigating the influence of age on patients with autoimmune gastritis (AIG).</p><p><strong>Methods: </strong>A total of 56 patients diagnosed with AIG at our hospital between January 2019 and December 2021 were included in this study. The participants were categorized into the elderly group (≥ 60 years old) and the nonelderly group. We analyzed the baseline characteristics, gastroscopy findings, and various laboratory examination parameters to see if age significantly influences the clinical manifestation of AIG patients.</p><p><strong>Results: </strong>During the study period, 32 and 24 patients were included in the elderly and nonelderly groups. Regarding baseline characteristics and symptoms, the nonelderly group showed a higher prevalence of acid reflux (12.5% vs. 6.3%, <i>p</i> < 0.05), a higher proportion of asymptomatic patients (8.3% vs. 3.1%, <i>p</i> = 0.046), and a higher prevalence of iron deficiency anemia (37.5% vs. 12.5%). Regarding laboratory examinations, the nonelderly group had a lower mean corpuscular volume (78.8 ± 7.9 vs. 89.2 ± 8.1 fL, <i>p</i> = 0.024), decreased serum ferritin levels ((24.8 ± 10.9 vs. 48.4 ± 13.1 ng/mL, <i>p</i> = 0.024), elevated serum vitamin B12 (92.3 ± 18.2 vs. 76.8 ± 12.9 pmol/L, <i>p</i> = 0.037), and a higher incidence of positive thyroid peroxidase antibody (28.2% vs. 12.5%, <i>p</i> = 0.024). However, the gastroscopy findings, including the incidence of proliferative polyps, neuroendocrine tumors, gastric intraepithelial neoplasia, and cancers, showed no significant difference between the two groups (<i>p</i> > 0.05).</p><p><strong>Conclusion: </strong>Nonelderly patients with AIG exhibit distinct clinical features compared to elderly patients. Large sample sizes with multiple centers involved in studies are required to verify our findings.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"4250339"},"PeriodicalIF":1.6,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12752861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145877661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total Neoadjuvant Chemotherapy Followed by Laparoscopic Radical Gastrectomy Versus Neoadjuvant Chemotherapy for Clinical Stage T4a-bN + M0 Proximal Gastric Cancer: A Single-Center Retrospective Data Analysis.","authors":"ChangRong Que, ShuangMing Lin, Wen Ye, RunSheng Lai, Run Xie, DongBo Xu","doi":"10.1155/grp/4020664","DOIUrl":"10.1155/grp/4020664","url":null,"abstract":"<p><strong>Background: </strong>While preoperative neoadjuvant chemotherapy (NT) followed by surgery has gained acceptance in the management of locally advanced gastric cancer (LAGC), there remains a paucity of studies examining the efficacy of total neoadjuvant chemotherapy (TNT) for LAGC. This study was aimed at addressing this gap by comparing the outcomes of patients with clinical stage T4a-bN + M0 proximal gastric cancer treated with TNT and NT. The investigation sought to provide valuable insights into the effectiveness of the TNT regimen in this specific clinical context.</p><p><strong>Methods: </strong>Retrospective analysis was conducted on the clinical data of patients diagnosed with proximal LAGC who underwent perioperative docetaxel, oxaliplatin, and fluorouracil (FLOT) chemotherapy followed by laparoscopic radical gastrectomy at Longyan First Hospital affiliated with Fujian Medical University. The study, spanning from January 2017 to December 2019, included 26 patients in the TNT group and 32 patients in the NT group. Comparative assessments were made regarding the outcomes of chemotherapy and surgery, as well as the 3-year disease-free survival (DFS) and overall survival (OS) between the two groups.</p><p><strong>Results: </strong>The TNT group demonstrated superiority over the NT group in terms of operation time and intraoperative blood loss. While no significant difference was observed in total postoperative complications between the two groups, the TNT group exhibited a more pronounced downstaging in T stage and a higher rate of pathological complete regression. The 3-year OS rate was notably higher in the TNT group at 61.5%, compared to 46.9% in the NT group. Similarly, the 3-year DFS rate favored the TNT group at 53.8%, surpassing the rate of 34.4% in the NT group.</p><p><strong>Conclusions: </strong>The TNT approach for LAGC had the potential to enhance tumor regression and increase the completion rate of chemotherapy. This strategy demonstrated a positive trend in long-term outcomes and introduced a novel treatment model.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"4020664"},"PeriodicalIF":1.6,"publicationDate":"2025-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12695407/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145742101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QingXia HuaYu Formula Alleviates Severe Acute Pancreatitis-Associated Intestinal Injury by Modulating Group 3 Innate Lymphoid Cells.","authors":"Yuyang Liu, Yishuang Tang, Yue Wu, Yujie Jiang, Liang Zhang, Mingxian Zheng, Yixuan Ye, Jing Kong, Xiaosu Wang, Lin Yuan, Feng He, Bingduo Zhou","doi":"10.1155/grp/6013880","DOIUrl":"10.1155/grp/6013880","url":null,"abstract":"<p><strong>Background: </strong>Severe acute pancreatitis (SAP), one of the common causes of acute abdomen in clinical practice, is characterized by acute and rapid onset as well as the systemic inflammatory response syndrome, which often leads to intestinal injury and high mortality. Recent studies indicate that innate lymphoid cells (ILCs) play an important role in the connection of SAP and intestinal injury. TCM, including the QingXia HuaYu (QXHY) formula, is known to benefit gastrointestinal dysfunction in SAP, but its mechanisms, especially regarding ILCs, are unclear.</p><p><strong>Methods: </strong>A mouse model of SAP was established by retrograde infusion of sodium taurocholate into the common bile duct of C57BL/6 mice. QXHY was orally administered three times postinfusion. Serum inflammatory markers, pancreatic myeloperoxidase, and histopathological changes in the mice with SAP with or without QXHY treatment were used to evaluate the pancreatic injury and the therapeutic effects of QXHY. Intestinal tight junctions, serum markers for mucosal injury, and inflammatory factors were used to analyze the effects of QXHY on intestinal injury in SAP mice. Flow cytometry and qRT-PCR were used to analyze ILC3s numbers and functions.</p><p><strong>Results: </strong>QXHY significantly reduced serum amylase, pancreatic myeloperoxidase, and improved histopathological manifestations in SAP mice. In addition, QXHY reduced intestinal damage, improved tight junction integrity, and lowered proinflammatory cytokines. Mechanistically, QXHY restored the diminished ROR<i>γ</i>t⁺-ILC3s in SAP mice and upregulated the expression of IL-22 (interleukin-22) and IL-17 (interleukin-17) mRNA.</p><p><strong>Conclusion: </strong>QXHY mitigates SAP-associated intestinal damage by enhancing ILC3 cells to regulate tissue injury and maintain homeostasis. Our data suggest that QXHY is a promising alternative and cost-friendly therapy for SAP-associated intestinal damage.</p>","PeriodicalId":12597,"journal":{"name":"Gastroenterology Research and Practice","volume":"2025 ","pages":"6013880"},"PeriodicalIF":1.4,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12680464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145700274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}