胶体金法检测粪便钙保护蛋白在克罗恩病筛查和诊断中的临床价值

IF 2 4区医学 Q3 GASTROENTEROLOGY & HEPATOLOGY

Gastroenterology Research and Practice Pub Date : 2023-11-09 DOI:10.1155/2023/8866828

Wangdong Zhang, Yanyun Fan, Meijun Chen

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Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were presented with 95% confidence intervals (CIs). Results. Patients with CD showed significantly higher FCP levels. Compared with the healthy population, when the FCP level cut-off was 15 μg/g and 60 μg/g, the sensitivity, specificity, PPV, and NPV for CD diagnosis were 98.3% (CI, 95.0%–100%) and 78.0% (CI, 67.4–88.6%), 84.3% (CI, 76.7%–91.8%) and 98.9% (CI, 96.7%–100%), 80.6% (CI, 71.5%–89.7%) and 97.9% (CI, 93.7%–100%), and 98.7% (CI, 96.2%–100%) and 87.1% (CI, 80.6%–93.6%), respectively. The AUCs were 0.969 (CI, 0.941–0.997). Compared with the gastrointestinal dysfunction group, using the same FCP level cut-off, the sensitivity, specificity, PPV, and NPV for CD diagnosis were 98.3% (CI, 95.0%–100%) and 78.0% (CI, 67.4%–88.6%), 71.1% (CI, 60.9%–81.3%) and 89.5% (CI, 82.3%–96.7%), 72.5% (CI, 62.7%–82.3%) and 85.2% (CI, 75.7%–94.7%), and 98.1% (CI, 94.5%–100%) and 84.0% (CI, 76.0%–92.0%), respectively. The AUCs were 0.908 (CI, 0.856–0.960). Conclusion. 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This study is aimed at using the colloidal gold semiquantitative assay to detect fecal calprotectin (FCP) and determine whether it is helpful in screening or diagnosing CD. Methods. Using a prospectively maintained database, 59 patients with CD were analyzed using FCP measurement. Subsequently, 76 patients and 89 healthy individuals were assigned to the gastrointestinal dysfunction and control groups, respectively. To aid in the screening or diagnosis of CD, the receiver operating characteristic curve was used to determine the diagnostic efficacy of FCP thresholds. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were presented with 95% confidence intervals (CIs). Results. Patients with CD showed significantly higher FCP levels. 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引用次数: 0

摘要

背景。克罗恩病(CD)是一种慢性炎症性疾病，其发病率逐渐增加。因此，采用一种简单方便的检测方法，尽早在自然人群中检测出乳糜泻至关重要。本研究旨在利用胶体金半定量法检测粪便钙保护蛋白(FCP)，以确定其对乳糜泻的筛查或诊断是否有帮助。使用前瞻性维护的数据库，使用FCP测量对59例CD患者进行分析。随后，76名患者和89名健康人分别被分配到胃肠道功能障碍组和对照组。为了帮助筛查或诊断CD，使用受试者工作特征曲线来确定FCP阈值的诊断效果。敏感性、特异性、阳性预测值(PPV)和阴性预测值(NPV)以95%置信区间(ci)表示。结果。乳糜泻患者FCP水平明显升高。与健康人群相比，当FCP水平临界值为15 μg/g和60 μg/g时，诊断CD的敏感性、特异性、PPV和NPV分别为98.3% (CI, 95.0% ~ 100%)和78.0% (CI, 66.4 ~ 88.6%)、84.3% (CI, 76.7% ~ 91.8%)和98.9% (CI, 96.7% ~ 100%)、80.6% (CI, 71.5% ~ 89.7%)和97.9% (CI, 93.7% ~ 100%)、98.7% (CI, 96.2% ~ 100%)和87.1% (CI, 80.6% ~ 93.6%)。auc为0.969 (CI, 0.941 ~ 0.997)。与胃肠功能障碍组相比，采用相同的FCP水平临界值，诊断CD的敏感性、特异性、PPV和NPV分别为98.3% (CI, 95.0% ~ 100%)和78.0% (CI, 67.4% ~ 88.6%)， 71.1% (CI, 60.9% ~ 81.3%)和89.5% (CI, 82.3% ~ 96.7%)， 72.5% (CI, 62.7% ~ 82.3%)和85.2% (CI, 75.7% ~ 94.7%)， 98.1% (CI, 94.5% ~ 100%)和84.0% (CI, 76.0% ~ 92.0%)。auc为0.908 (CI, 0.856 ~ 0.960)。结论。胶体金半定量法检测FCP可有效筛查和辅助诊断CD。

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Clinical Value of Detecting Fecal Calprotectin by Using Colloidal Gold Assay in Screening or Diagnosing Crohn’s Disease

Background. Crohn’s disease (CD) is a chronic inflammatory disease, and its incidence is gradually increasing. Thus, the use of a simple and convenient examination method to detect CD in the natural population as early as possible is crucial. This study is aimed at using the colloidal gold semiquantitative assay to detect fecal calprotectin (FCP) and determine whether it is helpful in screening or diagnosing CD. Methods. Using a prospectively maintained database, 59 patients with CD were analyzed using FCP measurement. Subsequently, 76 patients and 89 healthy individuals were assigned to the gastrointestinal dysfunction and control groups, respectively. To aid in the screening or diagnosis of CD, the receiver operating characteristic curve was used to determine the diagnostic efficacy of FCP thresholds. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were presented with 95% confidence intervals (CIs). Results. Patients with CD showed significantly higher FCP levels. Compared with the healthy population, when the FCP level cut-off was 15 μg/g and 60 μg/g, the sensitivity, specificity, PPV, and NPV for CD diagnosis were 98.3% (CI, 95.0%–100%) and 78.0% (CI, 67.4–88.6%), 84.3% (CI, 76.7%–91.8%) and 98.9% (CI, 96.7%–100%), 80.6% (CI, 71.5%–89.7%) and 97.9% (CI, 93.7%–100%), and 98.7% (CI, 96.2%–100%) and 87.1% (CI, 80.6%–93.6%), respectively. The AUCs were 0.969 (CI, 0.941–0.997). Compared with the gastrointestinal dysfunction group, using the same FCP level cut-off, the sensitivity, specificity, PPV, and NPV for CD diagnosis were 98.3% (CI, 95.0%–100%) and 78.0% (CI, 67.4%–88.6%), 71.1% (CI, 60.9%–81.3%) and 89.5% (CI, 82.3%–96.7%), 72.5% (CI, 62.7%–82.3%) and 85.2% (CI, 75.7%–94.7%), and 98.1% (CI, 94.5%–100%) and 84.0% (CI, 76.0%–92.0%), respectively. The AUCs were 0.908 (CI, 0.856–0.960). Conclusion. Detecting FCP by using the colloidal gold semiquantitative assay can be effective in screening and adjunct diagnosing of CD.

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来源期刊

Gastroenterology Research and Practice GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Gastroenterology Research and Practice is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on all areas of gastroenterology, hepatology, pancreas and biliary, and related cancers. The journal welcomes submissions on the physiology, pathophysiology, etiology, diagnosis and therapy of gastrointestinal diseases. The aim of the journal is to provide cutting edge research related to the field of gastroenterology, as well as digestive diseases and disorders. Topics of interest include: Management of pancreatic diseases Third space endoscopy Endoscopic resection Therapeutic endoscopy Therapeutic endosonography.