Frontiers in Neural Circuits最新文献_第3页

Anesthetized animal experiments for neuroscience research 用于神经科学研究的麻醉动物实验

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-31 DOI: 10.3389/fncir.2024.1426689

Shin Nagayama, Sanae Hasegawa-Ishii, Shu Kikuta

{"title":"Anesthetized animal experiments for neuroscience research","authors":"Shin Nagayama, Sanae Hasegawa-Ishii, Shu Kikuta","doi":"10.3389/fncir.2024.1426689","DOIUrl":"https://doi.org/10.3389/fncir.2024.1426689","url":null,"abstract":"Brain research has progressed with anesthetized animal experiments for a long time. Recent progress in research techniques allows us to measure neuronal activity in awake animals combined with behavioral tasks. The trends became more prominent in the last decade. This new research style triggers the paradigm shift in the research of brain science, and new insights into brain function have been revealed. It is reasonable to consider that awake animal experiments are more ideal for understanding naturalistic brain function than anesthetized ones. However, the anesthetized animal experiment still has advantages in some experiments. To take advantage of the anesthetized animal experiments, it is important to understand the mechanism of anesthesia and carefully handle the obtained data. In this minireview, we will shortly summarize the molecular mechanism of anesthesia in animal experiments, a recent understanding of the neuronal activities in a sensory system in the anesthetized animal brain, and consider the advantages and disadvantages of the anesthetized and awake animal experiments. This discussion will help us to use both research conditions in the proper manner.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endogenous opioids in the olfactory tubercle and their roles in olfaction and quality of life 嗅结节中的内源性阿片类物质及其在嗅觉和生活质量中的作用

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-30 DOI: 10.3389/fncir.2024.1408189

Koshi Murata, Ayako Maegawa, Yoshimasa Imoto, Shigeharu Fujieda, Yugo Fukazawa

{"title":"Endogenous opioids in the olfactory tubercle and their roles in olfaction and quality of life","authors":"Koshi Murata, Ayako Maegawa, Yoshimasa Imoto, Shigeharu Fujieda, Yugo Fukazawa","doi":"10.3389/fncir.2024.1408189","DOIUrl":"https://doi.org/10.3389/fncir.2024.1408189","url":null,"abstract":"Olfactory dysfunctions decrease daily quality of life (QOL) in part by reducing the pleasure of eating. Olfaction plays an essential role in flavor sensation and palatability. The decreased QOL due to olfactory dysfunction is speculated to result from abnormal neural activities in the olfactory and limbic areas of the brain, as well as peripheral odorant receptor dysfunctions. However, the specific underlying neurobiological mechanisms remain unclear. As the olfactory tubercle (OT) is one of the brain’s regions with high expression of endogenous opioids, we hypothesize that the mechanism underlying the decrease in QOL due to olfactory dysfunction involves the reduction of neural activity in the OT and subsequent endogenous opioid release in specialized subregions. In this review, we provide an overview and recent updates on the OT, the endogenous opioid system, and the pleasure systems in the brain and then discuss our hypothesis. To facilitate the effective treatment of olfactory dysfunctions and decreased QOL, elucidation of the neurobiological mechanisms underlying the pleasure of eating through flavor sensation is crucial.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141195901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shaping the olfactory map: cell type-specific activity patterns guide circuit formation 塑造嗅觉图谱：细胞类型特异性活动模式引导电路形成

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-27 DOI: 10.3389/fncir.2024.1409680

Ai Nakashima, Haruki Takeuchi

引用次数: 0

Connectivity of the olfactory tubercle: inputs, outputs, and their plasticity 嗅结节的连接性：输入、输出及其可塑性

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-22 DOI: 10.3389/fncir.2024.1423505

Masahiro Yamaguchi

引用次数: 0

Activity-dependent dendrite patterning in the postnatal barrel cortex 后天桶状皮层中依赖活动的树突模式化

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-17 DOI: 10.3389/fncir.2024.1409993

Naoki Nakagawa, Takuji Iwasato

{"title":"Activity-dependent dendrite patterning in the postnatal barrel cortex","authors":"Naoki Nakagawa, Takuji Iwasato","doi":"10.3389/fncir.2024.1409993","DOIUrl":"https://doi.org/10.3389/fncir.2024.1409993","url":null,"abstract":"For neural circuit construction in the brain, coarse neuronal connections are assembled prenatally following genetic programs, being reorganized postnatally by activity-dependent mechanisms to implement area-specific computational functions. Activity-dependent dendrite patterning is a critical component of neural circuit reorganization, whereby individual neurons rearrange and optimize their presynaptic partners. In the rodent primary somatosensory cortex (barrel cortex), driven by thalamocortical inputs, layer 4 (L4) excitatory neurons extensively remodel their basal dendrites at neonatal stages to ensure specific responses of barrels to the corresponding individual whiskers. This feature of barrel cortex L4 neurons makes them an excellent model, significantly contributing to unveiling the activity-dependent nature of dendrite patterning and circuit reorganization. In this review, we summarize recent advances in our understanding of the activity-dependent mechanisms underlying dendrite patterning. Our focus lays on the mechanisms revealed by in vivo time-lapse imaging, and the role of activity-dependent Golgi apparatus polarity regulation in dendrite patterning. We also discuss the type of neuronal activity that could contribute to dendrite patterning and hence connectivity.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140963004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of prenatal alcohol exposure on the olfactory system development 产前接触酒精对嗅觉系统发育的影响

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-15 DOI: 10.3389/fncir.2024.1408187

Fumiaki Imamura

{"title":"Effects of prenatal alcohol exposure on the olfactory system development","authors":"Fumiaki Imamura","doi":"10.3389/fncir.2024.1408187","DOIUrl":"https://doi.org/10.3389/fncir.2024.1408187","url":null,"abstract":"Fetal Alcohol Spectrum Disorders (FASD), resulting from maternal alcohol consumption during pregnancy, are a prominent non-genetic cause of physical disabilities and brain damage in children. Alongside common symptoms like distinct facial features and neurocognitive deficits, sensory anomalies, including olfactory dysfunction, are frequently noted in FASD-afflicted children. However, the precise mechanisms underpinning the olfactory abnormalities induced by prenatal alcohol exposure (PAE) remain elusive. Utilizing rodents as a model organism with varying timing, duration, dosage, and administration routes of alcohol exposure, prior studies have documented impairments in olfactory system development caused by PAE. Many reported a reduction in the olfactory bulb (OB) volume accompanied by reduced OB neuron counts, suggesting the OB is a brain region vulnerable to PAE. In contrast, no significant olfactory system defects were observed in some studies, though subtle alterations might exist. These findings suggest that the timing, duration, and extent of fetal alcohol exposure can yield diverse effects on olfactory system development. To enhance comprehension of PAE-induced olfactory dysfunctions, this review summarizes key findings from previous research on the olfactory systems of offspring prenatally exposed to alcohol.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140971698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hormonal and circuit mechanisms controlling female sexual behavior 控制女性性行为的荷尔蒙和回路机制

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-05-01 DOI: 10.3389/fncir.2024.1409349

Sayaka Inoue

引用次数: 0

Comparison of the connectivity of the posterior intralaminar thalamic nucleus and peripeduncular nucleus in rats and mice 比较大鼠和小鼠丘脑后束内核和周核的连接性

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-04-26 DOI: 10.3389/fncir.2024.1384621

Hui-Ru Cai, Sheng-Qiang Chen, Xiao-Jun Xiang, Xue-Qin Zhang, Run-Zhe Ma, Ge Zhu, Song-Lin Ding

{"title":"Comparison of the connectivity of the posterior intralaminar thalamic nucleus and peripeduncular nucleus in rats and mice","authors":"Hui-Ru Cai, Sheng-Qiang Chen, Xiao-Jun Xiang, Xue-Qin Zhang, Run-Zhe Ma, Ge Zhu, Song-Lin Ding","doi":"10.3389/fncir.2024.1384621","DOIUrl":"https://doi.org/10.3389/fncir.2024.1384621","url":null,"abstract":"The posterior intralaminar thalamic nucleus (PIL) and peripeduncular nucleus (PP) are two adjoining structures located medioventral to the medial geniculate nucleus. The PIL-PP region plays important roles in auditory fear conditioning and in social, maternal and sexual behaviors. Previous studies often lumped the PIL and PP into single entity, and therefore it is not known if they have common and/or different brain-wide connections. In this study, we investigate brain-wide efferent and afferent projections of the PIL and PP using reliable anterograde and retrograde tracing methods. Both PIL and PP project strongly to lateral, medial and anterior basomedial amygdaloid nuclei, posteroventral striatum (putamen and external globus pallidus), amygdalostriatal transition area, zona incerta, superior and inferior colliculi, and the ectorhinal cortex. However, the PP rather than the PIL send stronger projections to the hypothalamic regions such as preoptic area/nucleus, anterior hypothalamic nucleus, and ventromedial nucleus of hypothalamus. As for the afferent projections, both PIL and PP receive multimodal information from auditory (inferior colliculus, superior olivary nucleus, nucleus of lateral lemniscus, and association auditory cortex), visual (superior colliculus and ectorhinal cortex), somatosensory (gracile and cuneate nuclei), motor (external globus pallidus), and limbic (central amygdaloid nucleus, hypothalamus, and insular cortex) structures. However, the PP rather than PIL receives strong projections from the visual related structures parabigeminal nucleus and ventral lateral geniculate nucleus. Additional results from Cre-dependent viral tracing in mice have also confirmed the main results in rats. Together, the findings in this study would provide new insights into the neural circuits and functional correlation of the PIL and PP.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140805823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Location analysis of presynaptically active and silent synapses in single-cultured hippocampal neurons 单培养海马神经元突触前活跃和沉默突触的位置分析

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-04-23 DOI: 10.3389/fncir.2024.1358570

Otoya Kitaoka, Kohei Oyabu, Kaori Kubota, Takuya Watanabe, Satoru Kondo, Teppei Matsui, S. Katsurabayashi, Katsunori Iwasaki

{"title":"Location analysis of presynaptically active and silent synapses in single-cultured hippocampal neurons","authors":"Otoya Kitaoka, Kohei Oyabu, Kaori Kubota, Takuya Watanabe, Satoru Kondo, Teppei Matsui, S. Katsurabayashi, Katsunori Iwasaki","doi":"10.3389/fncir.2024.1358570","DOIUrl":"https://doi.org/10.3389/fncir.2024.1358570","url":null,"abstract":"A morphologically present but non-functioning synapse is termed a silent synapse. Silent synapses are categorized into “postsynaptically silent synapses,” where AMPA receptors are either absent or non-functional, and “presynaptically silent synapses,” where neurotransmitters cannot be released from nerve terminals. The presence of presynaptically silent synapses remains enigmatic, and their physiological significance is highly intriguing. In this study, we examined the distribution and developmental changes of presynaptically active and silent synapses in individual neurons. Our findings show a gradual increase in the number of excitatory synapses, along with a corresponding decrease in the percentage of presynaptically silent synapses during neuronal development. To pinpoint the distribution of presynaptically active and silent synapses, i.e., their positional information, we employed Sholl analysis. Our results indicate that the distribution of presynaptically silent synapses within a single neuron does not exhibit a distinct pattern during synapse development in different distance from the cell body. However, irrespective of neuronal development, the proportion of presynaptically silent synapses tends to rise as the projection site moves farther from the cell body, suggesting that synapses near the cell body may exhibit higher synaptic transmission efficiency. This study represents the first observation of changes in the distribution of presynaptically active and silent synapses within a single neuron.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140668481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunolabeling-compatible PEGASOS tissue clearing for high-resolution whole mouse brain imaging 用于高分辨率小鼠全脑成像的免疫标记兼容 PEGASOS 组织清除技术

IF 3.5 3区医学

Frontiers in Neural Circuits Pub Date : 2024-04-17 DOI: 10.3389/fncir.2024.1345692

Pan Gao, Matthew Rivera, Xiaoxiao Lin, Todd C. Holmes, Hu Zhao, Xiangmin Xu

{"title":"Immunolabeling-compatible PEGASOS tissue clearing for high-resolution whole mouse brain imaging","authors":"Pan Gao, Matthew Rivera, Xiaoxiao Lin, Todd C. Holmes, Hu Zhao, Xiangmin Xu","doi":"10.3389/fncir.2024.1345692","DOIUrl":"https://doi.org/10.3389/fncir.2024.1345692","url":null,"abstract":"Novel brain clearing methods revolutionize imaging by increasing visualization throughout the brain at high resolution. However, combining the standard tool of immunostaining targets of interest with clearing methods has lagged behind. We integrate whole-mount immunostaining with PEGASOS tissue clearing, referred to as iPEGASOS (immunostaining-compatible PEGASOS), to address the challenge of signal quenching during clearing processes. iPEGASOS effectively enhances molecular-genetically targeted fluorescent signals that are otherwise compromised during conventional clearing procedures. Additionally, we demonstrate the utility of iPEGASOS for visualizing neurochemical markers or viral labels to augment visualization that transgenic mouse lines cannot provide. Our study encompasses three distinct applications, each showcasing the versatility and efficacy of this approach. We employ whole-mount immunostaining to enhance molecular signals in transgenic reporter mouse lines to visualize the whole-brain spatial distribution of specific cellular populations. We also significantly improve the visualization of neural circuit connections by enhancing signals from viral tracers injected into the brain. Last, we show immunostaining without genetic markers to selectively label beta-amyloid deposits in a mouse model of Alzheimer’s disease, facilitating the comprehensive whole-brain study of pathological features.","PeriodicalId":12498,"journal":{"name":"Frontiers in Neural Circuits","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0