G3: Genes|Genomes|Genetics最新文献_第2页

Using feedback in pooled experiments augmented with imputation for high genotyping accuracy at reduced cost.

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-23 DOI: 10.1093/g3journal/jkaf010

Camille Clouard, Carl Nettelblad

{"title":"Using feedback in pooled experiments augmented with imputation for high genotyping accuracy at reduced cost.","authors":"Camille Clouard, Carl Nettelblad","doi":"10.1093/g3journal/jkaf010","DOIUrl":"https://doi.org/10.1093/g3journal/jkaf010","url":null,"abstract":"Conducting genomic selection in plant breeding programs can substantially speed up the development of new varieties. Genomic selection provides more reliable insights when it is based on dense marker data, in which the rare variants can be particularly informative. Despite the availability of new technologies, the cost of large-scale genotyping remains a major limitation to the implementation of genomic selection. We suggest to combine pooled genotyping with population-based imputation as a cost-effective computational strategy for genotyping SNPs. Pooling saves genotyping tests and has proven to accurately capture the rare variants that are usually missed by imputation. In this study, we investigate adding iterative coupling to a joint model of pooling and imputation that we have previously proposed. In each iteration, the imputed genotype probabilities serve as feedback input for adjusting the per-sample prior genotype probabilities, before running a new imputation based on these adjusted data. This flexible setup indirectly imposes consistency between the imputed genotypes and the pooled observations. We demonstrate that repeated cycles of feedback can take advantage of the strengths in both pooling and imputation when an appropriate set of reference haplotypes is available for imputation. The iterations improve greatly upon the initial genotype predictions, achieving very high genotype accuracy for both low and high frequency variants. We enhance the average concordance from 94.5% to 98.4% at limited computational cost and without requiring any additional genotype testing.","PeriodicalId":12468,"journal":{"name":"G3: Genes|Genomes|Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental and conditional regulation of DAF-2/INSR ubiquitination in Caenorhabditis elegans. 秀丽隐杆线虫DAF-2/INSR泛素化的发育和条件调控

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-22 DOI: 10.1093/g3journal/jkaf009

Ivan B Falsztyn, Seth M Taylor, L Ryan Baugh

{"title":"Developmental and conditional regulation of DAF-2/INSR ubiquitination in Caenorhabditis elegans.","authors":"Ivan B Falsztyn, Seth M Taylor, L Ryan Baugh","doi":"10.1093/g3journal/jkaf009","DOIUrl":"10.1093/g3journal/jkaf009","url":null,"abstract":"Insulin/IGF signaling (IIS) regulates developmental and metabolic plasticity. Conditional regulation of insulin-like peptide expression and secretion promotes different phenotypes in different environments. However, IIS can also be regulated by other, less-understood mechanisms. For example, stability of the only known insulin/IGF receptor in C. elegans, DAF-2/INSR, is regulated by CHIP-dependent ubiquitination. Disruption of chn-1/CHIP reduces longevity in C. elegans by increasing DAF-2/INSR abundance and IIS activity in adults. Likewise, mutation of a ubiquitination site causes daf-2(gk390525) to display gain-of-function phenotypes in adults. However, we show that this allele displays loss-of-function phenotypes in larvae, and that its effect on IIS activity transitions from negative to positive during development. In contrast, the allele acts like a gain-of-function in larvae cultured at high temperature, inhibiting temperature-dependent dauer formation. Disruption of chn-1/CHIP causes an increase in IIS activity in starved L1 larvae, unlike daf-2(gk390525). CHN-1/CHIP ubiquitinates DAF-2/INSR at multiple sites. These results suggest that the sites that are functionally relevant to negative regulation of IIS vary in larvae and adults, at different temperatures, and in nutrient-dependent fashion, revealing additional layers of IIS regulation.","PeriodicalId":12468,"journal":{"name":"G3: Genes|Genomes|Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143003260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference genome of Calochortus tolmiei Hook. & Arn. (Liliaceae), a cat's ear mariposa lily. 托氏蛤蛤的参考基因组。和攻击。（百合科），一种猫耳百合。

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-20 DOI: 10.1093/g3journal/jkaf008

Jacob B Landis, Julianna J Harden, Evan Eifler, Gretta Buttleman, Adriana I Hernandez, Thomas J Givnish, Susan R Strickler, Chelsea D Specht

引用次数: 0

Functional test of a naturally occurred tumor modifier gene provides insights to melanoma development. 自然发生的肿瘤修饰基因的功能测试为黑色素瘤的发展提供了见解。

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-17 DOI: 10.1093/g3journal/jkae298

Mateo Garcia-Olazabal, Mateus Contar Adolfi, Brigitta Wilde, Anita Hufnagel, Rupesh Paudel, Yuan Lu, Svenja Meierjohann, Gil G Rosenthal, Manfred Schartl

{"title":"Functional test of a naturally occurred tumor modifier gene provides insights to melanoma development.","authors":"Mateo Garcia-Olazabal, Mateus Contar Adolfi, Brigitta Wilde, Anita Hufnagel, Rupesh Paudel, Yuan Lu, Svenja Meierjohann, Gil G Rosenthal, Manfred Schartl","doi":"10.1093/g3journal/jkae298","DOIUrl":"https://doi.org/10.1093/g3journal/jkae298","url":null,"abstract":"Occurrence of degenerative interactions is thought to serve as a mechanism underlying hybrid unfitness in most animal systems. However, the molecular mechanisms underpinning the genetic interaction and how they contribute to overall hybrid incompatibilities are limited to only a handful of examples. A vertebrate model organism, Xiphophorus, is used to study hybrid dysfunction, and it has been shown from this model that diseases, such as melanoma, can occur in certain interspecies hybrids. Melanoma development is due to hybrid inheritance of an oncogene, xmrk, and loss of a co-evolved tumor modifier. It was recently found that adgre5, a G protein-coupled receptor involved in cell adhesion, is a tumor regulator gene in naturally hybridizing Xiphophorus species Xiphophorus birchmanni (X. birchmanni) and Xiphophorus malinche (X. malinche). We hypothesized that 1 of the 2 parental alleles of adgre5 is involved in regulation of cell growth, migration, and melanomagenesis. Accordingly, we assessed the function of adgre5 alleles from each parental species of the melanoma-bearing hybrids using in vitro cell growth and migration assays. In addition, we expressed each adgre5 allele with the xmrk oncogene in transgenic medaka. We found that cells transfected with the X. birchmanni adgre5 exhibited decreased growth and migration compared to those with the X. malinche allele. Moreover, X. birchmanni allele of adgre5 completely inhibited melanoma development in xmrk-transgenic medaka, while X. malinche adgre5 expression did not exhibit melanoma suppressive activity in medaka. These findings provide evidence that adgre5 is a natural melanoma suppressor and provide new insight in melanoma etiology.","PeriodicalId":12468,"journal":{"name":"G3: Genes|Genomes|Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143003261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GWAS-Assisted and multi-trait genomic prediction for improvement of seed yield and canning quality traits in a black bean breeding panel. gwas辅助多性状基因组预测黑豆种子产量和罐头品质性状的改良。

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-16 DOI: 10.1093/g3journal/jkaf007

Paulo Izquierdo, Evan M Wright, Karen Cichy

{"title":"GWAS-Assisted and multi-trait genomic prediction for improvement of seed yield and canning quality traits in a black bean breeding panel.","authors":"Paulo Izquierdo, Evan M Wright, Karen Cichy","doi":"10.1093/g3journal/jkaf007","DOIUrl":"https://doi.org/10.1093/g3journal/jkaf007","url":null,"abstract":"In recent years, black beans (Phaseolus vulgaris L.) have gained popularity in the U.S., with improved seed yield and canning quality being critical traits for new cultivars. Achieving genetic gains in these traits is often challenging due to negative trait associations and the need for specialized equipment and trained sensory panels for evaluation. This study investigates the integration of genomics and phenomics to enhance selection accuracy for these complex traits. We evaluated the prediction accuracy of single and multi-trait genomic prediction (GP) models, incorporating near-infrared spectroscopy (NIRS) data and markers identified through genome-wide association studies (GWAS). The models demonstrated moderate prediction accuracies for yield and canning appearance, and high accuracies for color retention. No significant differences were found between single-trait and multi-trait models within the same breeding cycle. However, across breeding cycles, multi-trait models outperformed single-trait models by up to 45% and 63% for canning appearance and seed yield, respectively. Interestingly, incorporating significant SNP markers identified by GWAS and NIRS data into the models tended to decrease prediction accuracy both within and between breeding cycles. As genotypes from the new breeding cycle were included, the models' prediction accuracy generally increased. Our findings underscore the potential of multi-trait models to enhance the prediction of complex traits such as seed yield and canning quality in dry beans and highlight the importance of continually updating the training dataset for effective GP implementation in dry bean breeding.","PeriodicalId":12468,"journal":{"name":"G3: Genes|Genomes|Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143003263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell integrity limits ploidy in budding yeast. 细胞完整性限制了出芽酵母的倍性。

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-13 DOI: 10.1093/g3journal/jkae286

Juliet Barker, Andrew Murray, Stephen P Bell

{"title":"Cell integrity limits ploidy in budding yeast.","authors":"Juliet Barker, Andrew Murray, Stephen P Bell","doi":"10.1093/g3journal/jkae286","DOIUrl":"https://doi.org/10.1093/g3journal/jkae286","url":null,"abstract":"Evidence suggests that increases in ploidy have occurred frequently in the evolutionary history of organisms and can serve adaptive functions to specialized somatic cells in multicellular organisms. However, the sudden multiplication of all chromosome content may present physiological challenges to the cells in which it occurs. Experimental studies have associated increases in ploidy with reduced cell survival and proliferation. To understand the physiological challenges that suddenly increased chromosome content imposes on cells, we used S. cerevisiae to ask how much chromosomal DNA cells may contain and what determines this limit. We generated polyploid cells using 2 distinct methods causing cells to undergo endoreplication and identified the maximum ploidy of these cells, 32-64C. We found that physical determinants that alleviate or exacerbate cell surface stress increase and decrease the limit to ploidy, respectively. We also used these cells to investigate gene expression changes associated with increased ploidy and identified the repression of genes involved in ergosterol biosynthesis. We propose that ploidy is inherently limited by the impacts of growth in size, which accompany whole-genome duplication, to cell surface integrity.","PeriodicalId":12468,"journal":{"name":"G3: Genes|Genomes|Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new Drosophila melanogaster research resource: CRISPR-induced mutations for clonal analysis of fourth chromosome genes. 一种新的黑腹果蝇研究资源：crispr诱导的第四染色体基因克隆分析突变。

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-13 DOI: 10.1093/g3journal/jkaf006

Bonnie M Weasner, Brandon P Weasner, Kevin R Cook, Michael J Stinchfield, Shu Kondo, Kuniaki Saito, Justin P Kumar, Stuart J Newfeld

{"title":"A new Drosophila melanogaster research resource: CRISPR-induced mutations for clonal analysis of fourth chromosome genes.","authors":"Bonnie M Weasner, Brandon P Weasner, Kevin R Cook, Michael J Stinchfield, Shu Kondo, Kuniaki Saito, Justin P Kumar, Stuart J Newfeld","doi":"10.1093/g3journal/jkaf006","DOIUrl":"https://doi.org/10.1093/g3journal/jkaf006","url":null,"abstract":"As part of an ongoing effort to generate comprehensive resources for the experimental analysis of fourth chromosome genes in Drosophila melanogaster, the Fourth Chromosome Resource Project has used CRISPR mutagenesis with single guide RNAs to isolate mutations in 62 of the 80 fourth chromosome, protein-coding genes. These mutations were induced on a fourth chromosome bearing a basal FRT insertion to facilitate experimental approaches involving FLP recombinase-induced mitotic recombination. To permit straightforward comparisons among mutant stocks, most of the mutations were generated on isogenic fourth chromosomes, which were then crossed into a common genetic background. Of the 119 mutations, 84 are frameshift mutations likely to be null alleles, 29 are small, in-frame deletions and 6 have yet to be characterized molecularly. The mutations were tested for recessive lethal, female sterile and visible phenotypes. Stable stocks for most of the mutations have been submitted to repositories in the United States and Japan for public distribution.","PeriodicalId":12468,"journal":{"name":"G3: Genes|Genomes|Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptome profiling of maize transcription factor mutants to probe gene regulatory network predictions. 玉米转录因子突变体的转录组图谱分析，探索基因调控网络预测。

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-08 DOI: 10.1093/g3journal/jkae274

Erika L Ellison, Peng Zhou, Yi-Hsuan Chu, Peter Hermanson, Lina Gomez-Cano, Zachary A Myers, Ankita Abnave, John Gray, Candice N Hirsch, Erich Grotewold, Nathan M Springer

{"title":"Transcriptome profiling of maize transcription factor mutants to probe gene regulatory network predictions.","authors":"Erika L Ellison, Peng Zhou, Yi-Hsuan Chu, Peter Hermanson, Lina Gomez-Cano, Zachary A Myers, Ankita Abnave, John Gray, Candice N Hirsch, Erich Grotewold, Nathan M Springer","doi":"10.1093/g3journal/jkae274","DOIUrl":"10.1093/g3journal/jkae274","url":null,"abstract":"Transcription factors play important roles in regulation of gene expression and phenotype. A variety of approaches have been utilized to develop gene regulatory networks to predict the regulatory targets for each transcription factor, such as yeast-1-hybrid screens and gene co-expression network analysis. Here we identified potential transcription factor targets and used a reverse genetics approach to test the predictions of several gene regulatory networks in maize. Loss-of-function mutant alleles were isolated for 22 maize transcription factors. These mutants did not exhibit obvious morphological phenotypes. However, transcriptomic profiling identified differentially expressed genes in each of the mutant genotypes, and targeted metabolic profiling indicated variable phenolic accumulation in some mutants. An analysis of expression levels for predicted target genes based on yeast-1-hybrid screens identified a small subset of predicted targets that exhibit altered expression levels. The analysis of predicted targets from gene co-expression network-based methods found significant enrichments for prediction sets of some transcription factors, but most predicted targets did not exhibit altered expression. This could result from false-positive gene co-expression network predictions, a transcription factor with a secondary regulatory role resulting in minor effects on gene regulation, or redundant gene regulation by other transcription factors. Collectively, these findings suggest that loss-of-function for single uncharacterized transcription factors might have limited phenotypic impacts but can reveal subsets of gene regulatory network predicted targets with altered expression.","PeriodicalId":12468,"journal":{"name":"G3: Genes|Genomes|Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The genome of the cryopelagic Antarctic bald notothen, Trematomus borchgrevinki. 低温深海南极秃艽--Trematomus borchgrevinki 的基因组。

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-08 DOI: 10.1093/g3journal/jkae267

Niraj Rayamajhi, Angel G Rivera-Colón, Bushra Fazal Minhas, C H Christina Cheng, Julian M Catchen

{"title":"The genome of the cryopelagic Antarctic bald notothen, Trematomus borchgrevinki.","authors":"Niraj Rayamajhi, Angel G Rivera-Colón, Bushra Fazal Minhas, C H Christina Cheng, Julian M Catchen","doi":"10.1093/g3journal/jkae267","DOIUrl":"10.1093/g3journal/jkae267","url":null,"abstract":"The Antarctic bald notothen, Trematomus borchgrevinki (family Nototheniidae) occupies a high latitude, ice-laden environment and represents an extreme example of cold-specialization among fishes. We present the first, high-quality, chromosome-scale genome of a female T. borchgrevinki individual comprised of 23 putative chromosomes, the largest of which is 65 megabasepairs (Mbp) in length. The total length of the genome 935.13 Mbp, composed of 2,094 scaffolds, with a scaffold N50 of 42.67 Mbp. Annotation yielded 22,192 protein-coding genes while 54.75% of the genome was occupied by repetitive elements; an analysis of repeats demonstrated that an expansion occurred in recent time. Conserved synteny analysis revealed that the genome architecture of T. borchgrevinki is largely maintained with other members of the notothenioid clade, although several significant translocations and inversions are present, including the fusion of orthologous chromosomes 8 and 11 into a single element. This genome will serve as a cold-specialized model for comparisons to other members of the notothenioid adaptive radiation.","PeriodicalId":12468,"journal":{"name":"G3: Genes|Genomes|Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708224/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic screening reveals cone cell-specific factors as common genetic targets modulating rival-induced prolonged mating in male Drosophila melanogaster. 基因筛选发现锥状细胞特异性因子是调节雄性黑腹果蝇竞争诱导交配时间延长的共同基因靶标

IF 2.1 3区生物学

G3: Genes|Genomes|Genetics Pub Date : 2025-01-08 DOI: 10.1093/g3journal/jkae255

Yanying Sun, Xiaoli Zhang, Zekun Wu, Wenjing Li, Woo Jae Kim

{"title":"Genetic screening reveals cone cell-specific factors as common genetic targets modulating rival-induced prolonged mating in male Drosophila melanogaster.","authors":"Yanying Sun, Xiaoli Zhang, Zekun Wu, Wenjing Li, Woo Jae Kim","doi":"10.1093/g3journal/jkae255","DOIUrl":"10.1093/g3journal/jkae255","url":null,"abstract":"Male-male social interactions exert a substantial impact on the transcriptional regulation of genes associated with aggression and mating behavior in male Drosophila melanogaster. Throughout our comprehensive genetic screening of aggression-related genes, we identified that the majority of mutants for these genes are associated with rival-induced and visually oriented mating behavior, longer-mating duration (LMD). The majority of mutants with upregulated genes in single-housed males significantly altered LMD behavior but not copulation latency, suggesting a primary regulation of mating duration. Single-cell RNA-sequencing revealed that LMD-related genes are predominantly co-expressed with male-specific genes like dsx and Cyp6a20 in specific cell populations, especially in cone cells. Functional validation confirmed the roles of these genes in mediating LMD. Expression of LMD genes like Cyp6a20, Cyp4d21, and CrzR was enriched in cone cells, with disruptions in cone cell-specific expression of CrzR and Cyp4d21 leading to disrupted LMD. We also identified a novel gene, CG10026/Macewindu, that reversed LMD when overexpressed in cone cells. These findings underscore the critical role of cone cells as a pivotal site for the expression of genes involved in the regulation of LMD behavior. This study provides valuable insights into the intricate mechanisms underlying complex sexual behaviors in Drosophila.","PeriodicalId":12468,"journal":{"name":"G3: Genes|Genomes|Genetics","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11708226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142566341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0