Frontiers in Cell and Developmental Biology最新文献

{"title":"Color fundus photograph-based diabetic retinopathy grading via label relaxed collaborative learning on deep features and radiomics features.","authors":"Chao Zhang, Guanglei Sheng, Jie Su, Lian Duan","doi":"10.3389/fcell.2024.1513971","DOIUrl":"10.3389/fcell.2024.1513971","url":null,"abstract":"<p><strong>Introduction: </strong>Diabetic retinopathy (DR) has long been recognized as a common complication of diabetes, making accurate automated grading of its severity essential. Color fundus photographs play a crucial role in the grading of DR. With the advancement of artificial intelligence technologies, numerous researchers have conducted studies on DR grading based on deep features and radiomic features extracted from color fundus photographs.</p><p><strong>Method: </strong>We combine deep features and radiomic features to design a feature fusion algorithm. First, we utilize convolutional neural networks to extract deep features from color fundus photographs and employ radiomic methodologies to extract radiomic features. Subsequently, we design a label relaxation-based collaborative learning algorithm for feature fusion.</p><p><strong>Results: </strong>We validate the effectiveness of the proposed method on two fundus image datasets: the DR1 Dataset and the MESSIDOR Dataset. The proposed method achieved 96.86 of AUC on DR1 and 96.34 of AUC on MESSIDOR, which are better than state-of-the-art methods. Also, the divergence between the training AUC and testing AUC increases substantially after the removal of manifold regularization.</p><p><strong>Conclusion: </strong>Label relaxation can enhance the distinguishability of training samples in the label space, thereby improving the model's classification accuracy. Additionally, graph constraints based on manifold learning methods can mitigate overfitting caused by label relaxation.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1513971"},"PeriodicalIF":4.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes: new targets for understanding axon guidance in the developing central nervous system.","authors":"Mingyu Liu, Teng Teng","doi":"10.3389/fcell.2024.1510862","DOIUrl":"10.3389/fcell.2024.1510862","url":null,"abstract":"<p><p>Axon guidance is a key event in neural circuit development that drives the correct targeting of axons to their targets through long distances and unique patterns. Exosomes, extracellular vesicles that are smaller than 100 nm, are secreted by most cell types in the brain. Regulation of cell-cell communication, neuroregeneration, and synapse formation by exosomes have been extensively studied. However, the interaction between exosomes and axon guidance molecules is poorly understood. This review summarizes the relationship between exosomes and canonical and non-canonical guidance cues and hypothesizes a possible model for exosomes mediating axon guidance between cells. The roles of exosomes in axon outgrowth, regeneration, and neurodevelopmental disorders are also reviewed, to discuss exosome-guidance interactions as potential clinical therapeutic targets.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1510862"},"PeriodicalIF":4.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The quagga mussel, <i>Dreissena rostriformis</i>: a novel model for EcoEvoDevo, environmental research, and the applied sciences.","authors":"Andreas Wanninger, Gudrun Schwarze","doi":"10.3389/fcell.2024.1531560","DOIUrl":"10.3389/fcell.2024.1531560","url":null,"abstract":"<p><p>Bivalve mollusks are globally distributed in marine and freshwater habitats. While exhibiting a relatively uniform bodyplan that is characterized by their eponymous bivalved shell that houses the soft-bodied animal, many lineages have acquired unique morphological, physiological, and molecular innovations that account for their high adaptability to the various properties of aquatic environments such as salinity, flow conditions, or substrate composition. This renders them ideal candidates for studies into the evolutionary trajectories that have resulted in their diversity, but also makes them important players for research concerned with climate change-induced warming and acidification of aquatic habitats. Some species, such as the blue and Mediterranean as well as the zebra and quagga mussels, form biodegradable fibers, the byssus threads. These have significant potential for biomimetic approaches by aiding in developing sustainable textiles and other fiber-based fabrics. Despite this broad span of scientific relevance, bivalves remain dramatically understudied and key resources such as high-quality genomes and developmental transcriptomes in combination with established laboratory protocols to carry out state-of-the-art molecular and morphological studies are only available for less than a handful of species. Here, we report on one of the best-investigated bivalves in this respect, the quagga mussel, <i>Dreissena rostriformis</i>, an invasive freshwater species. We summarize the current state of knowledge and available resources that make the quagga mussel highly amenable for studying adaptive mechanisms for life in hypoosmotic environments, biomineralization, biomimetics, and evolutionary developmental biology. We argue that the unique combination of biological features and the broad relevance of the quagga mussel for the basic and the applied sciences as well as for biomonitoring and conservation biology measures call for intensified research efforts using <i>Dreissena rostriformis</i> as a model.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1531560"},"PeriodicalIF":4.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11754198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multi-modal multi-branch framework for retinal vessel segmentation using ultra-widefield fundus photographs.","authors":"Qihang Xie, Xuefei Li, Yuanyuan Li, Jiayi Lu, Shaodong Ma, Yitian Zhao, Jiong Zhang","doi":"10.3389/fcell.2024.1532228","DOIUrl":"10.3389/fcell.2024.1532228","url":null,"abstract":"<p><strong>Background: </strong>Vessel segmentation in fundus photography has become a cornerstone technique for disease analysis. Within this field, Ultra-WideField (UWF) fundus images offer distinct advantages, including an expansive imaging range, detailed lesion data, and minimal adverse effects. However, the high resolution and low contrast inherent to UWF fundus images present significant challenges for accurate segmentation using deep learning methods, thereby complicating disease analysis in this context.</p><p><strong>Methods: </strong>To address these issues, this study introduces M3B-Net, a novel multi-modal, multi-branch framework that leverages fundus fluorescence angiography (FFA) images to improve retinal vessel segmentation in UWF fundus images. Specifically, M3B-Net tackles the low segmentation accuracy caused by the inherently low contrast of UWF fundus images. Additionally, we propose an enhanced UWF-based segmentation network in M3B-Net, specifically designed to improve the segmentation of fine retinal vessels. The segmentation network includes the Selective Fusion Module (SFM), which enhances feature extraction within the segmentation network by integrating features generated during the FFA imaging process. To further address the challenges of high-resolution UWF fundus images, we introduce a Local Perception Fusion Module (LPFM) to mitigate context loss during the segmentation cut-patch process. Complementing this, the Attention-Guided Upsampling Module (AUM) enhances segmentation performance through convolution operations guided by attention mechanisms.</p><p><strong>Results: </strong>Extensive experimental evaluations demonstrate that our approach significantly outperforms existing state-of-the-art methods for UWF fundus image segmentation.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1532228"},"PeriodicalIF":4.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on gene expression in the liver at various developmental stages of human embryos.","authors":"Hanqing Chen, Tingting Li, Ming Cai, Zhiqi Huang, Jianjun Gao, Hongping Ding, Minmin Li, Weiyu Guan, Jinpeng Chen, Wenran Wang, Chunhong Li, Jianwu Shi","doi":"10.3389/fcell.2024.1515524","DOIUrl":"10.3389/fcell.2024.1515524","url":null,"abstract":"<p><strong>Background: </strong>The normal development of the liver during human embryonic stages is critical for the functionality of the adult liver. Despite this, the essential genes, biological processes, and signal pathways that drive liver development in human embryos remain poorly understood.</p><p><strong>Methods: </strong>In this study, liver samples were collected from human embryos at progressive developmental stages, ranging from 2-month-old to 7-month-old. Highly expressed genes and their associated enrichment processes at various developmental stages of the liver were identified through transcriptomic sequencing.</p><p><strong>Results: </strong>The findings indicated that genes associated with humoral immune responses and B-cell-mediated immunity were highly expressed during the early developmental stages. Concurrently, numerous genes related to vitamin response, brown adipocyte differentiation, T cell differentiation, hormone secretion, hemostasis, peptide hormone response, steroid metabolism, and hematopoietic regulation exhibited increased expression aligned with liver development. Our results suggest that the liver may possess multiple functions during embryonic stages, beyond serving hematopoietic roles. Moreover, this study elucidated the complex regulatory interactions among genes involved in lymphocyte differentiation, the regulation of hemopoiesis, and liver development. Consequently, the development of human embryonic liver necessitates the synergistic regulation of numerous genes. Notably, alongside conventionally recognized genes, numerous previously uncharacterized genes involved in liver development and function were also identified.</p><p><strong>Conclusion: </strong>These findings establish a critical foundation for future research on liver development and diseases arising from fetal liver abnormalities.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1515524"},"PeriodicalIF":4.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the effect of gestational diabetes mellitus on retinal vascular morphology by PKSEA-Net.","authors":"Ligang Jiang, Yimei Ji, Mengting Liu, Ruolin Fang, Zhentao Zhu, Meizhen Zhang, Yuhua Tong","doi":"10.3389/fcell.2024.1532939","DOIUrl":"10.3389/fcell.2024.1532939","url":null,"abstract":"<p><strong>Background: </strong>Gestational diabetes mellitus (GDM) is a temporary metabolic disorder in which small retinal vessels may have experience subtle changes before clinical lesions of the fundus retina appear. An innovative artificial intelligence image processing technology was applied to locate and analyze the small retinal vessel morphology and accurately evaluate the changes of the small retinal vessels in GDM patients and pregnant women with normal blood glucose and non-pregnant women with normal blood glucose.</p><p><strong>Methods: </strong>The subjects were divided into three groups:GDM group, pregnant control group (PC), and normal control group (NC). Use optical coherence tomography angiography (OCTA) to collect OCT images of subjects,and perform quantitative identification and analysis of retinal vessel parameters based on artificial intelligence measurement software integrated the prior knowledge supervised edge-aware multi-task network (PKSEA-Net): Retinal arteriolar lumen diameter (RALD), retinal arteriolar outer diameter (RAOD), retinal venular lumen diameter (RVLD),retinal venular outer diameter (RVOD),arterial wall thickness (AWT),venular wall thickness (VWT),arterial wall to lumen ratio (AWLR),venular wall to lumen ratio (VWLR),arterial wall cross-sectional area (AWCSA),venular wall cross-sectional area (VWCSA), arteriovenous ratio (AVR).</p><p><strong>Results: </strong>This study revealed significant differences in RVOD, RVLD, VWT, VWCSA and AVR between the GDM group and the PC group (<i>p</i> = 0.005, <i>p</i> < 0.027, <i>p</i> = 0.008, <i>p</i> = 0.001, <i>p</i> = 0.022), significant differences in RVOD, RVLD, VWT, VWCSA and AVR between the GDM group and the NC group (<i>p</i> < 0.001, <i>p</i> = 0.001, <i>p</i> < 0.001, <i>p</i> < 0.001, <i>p</i> = 0.001). In GDM group, RVOD, RVLD, VWT and VWCSA increased, while AVR decreased. There were no significant differences in RVOD, RVLD, VWT, VWCSA and AVR between PC group and NC group (<i>p</i> = 0.139, <i>p</i> = 0.263, <i>p</i> = 0.107, <i>p</i> = 0.059, <i>p</i> = 0.218), and no significant differences in VWLR among the three groups (<i>p</i> > 0.05). No significant difference was observed in retinal artery vascular parameters (RAOD, RALD, AWT, AWLR, AWCSA) across the three groups (<i>p</i> > 0.05).</p><p><strong>Conclusion: </strong>There were increases in RVOD, RVLD, VWT, and VWCSA, decrease in AVR in patients with GDM. However, no significant difference of retinal vascular parameters was shown between normal pregnant women and normal non-pregnant women. PKSEA-Net can assist to identify changes in retinal vascular morphology and diagnose micro-vascular lesion early in normal pregnant women and high-risk groups of GDM.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1532939"},"PeriodicalIF":4.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How close is autophagy-targeting therapy for Alzheimer's disease to clinical use? A summary of autophagy modulators in clinical studies.","authors":"Sofia Miranda Fernandes, Johanna Mayer, Per Nilsson, Makoto Shimozawa","doi":"10.3389/fcell.2024.1520949","DOIUrl":"10.3389/fcell.2024.1520949","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a neurodegenerative disorder clinically characterized by progressive decline of memory and cognitive functions, and it is the leading cause of dementia accounting for 60%-80% of dementia patients. A pathological hallmark of AD is the accumulation of aberrant protein/peptide aggregates such as extracellular amyloid plaques containing amyloid-beta peptides and intracellular neurofibrillary tangles composed of hyperphosphorylated tau. These aggregates result from the failure of the proteostasis network, which encompasses protein synthesis, folding, and degradation processes. Autophagy is an intracellular self-digesting system responsible for the degradation of protein aggregates and damaged organelles. Impaired autophagy is observed in most neurodegenerative disorders, indicating the link between autophagy dysfunction and these diseases. A massive accumulation of autophagic vacuoles in neurons in Alzheimer's brains evidences autophagy impairment in AD. Modulating autophagy has been proposed as a therapeutic strategy for AD because of its potential to clear aggregated proteins. However, autophagy modulation therapy for AD is not yet clinically available. This mini-review aims to summarize clinical studies testing potential autophagy modulators for AD and to evaluate their proximity to clinical use. We accessed clinicaltrials.gov provided by the United States National Institutes of Health to identify completed and ongoing clinical trials. Additionally, we discuss the limitations and challenges of these therapies.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1520949"},"PeriodicalIF":4.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term dynamics of placozoan culture: emerging models for population and space biology.","authors":"Daria Y Romanova, Alexander A Povernov, Mikhail A Nikitin, Simkha I Borman, Yana A Frank, Leonid L Moroz","doi":"10.3389/fcell.2024.1514553","DOIUrl":"10.3389/fcell.2024.1514553","url":null,"abstract":"<p><p>As the simplest free-living animal, <i>Trichoplax adhaerens</i> (Placozoa) is emerging as a powerful paradigm to decipher molecular and cellular bases of behavior, enabling integrative studies at all levels of biological organization in the context of metazoan evolution and parallel origins of neural organization. However, the progress in this direction also depends on the ability to maintain a long-term culture of placozoans. Here, we report the dynamic of <i>Trichoplax</i> cultures over 11 years of observations from a starting clonal line, including 7 years of culturing under antibiotic (ampicillin) treatment. This study revealed very complex population dynamics, with seasonal oscillation and at least partial correlations with the solar radio emission flux and the magnetic field disturbance parameters. Notable, the analysis of the distribution of Fe²⁺ in living animals revealed not only its high abundance across most cells but also asymmetric localizations of Fe²⁺ in unidentified cells, suggesting that these Fe²⁺ intracellular patterns might be coupled with the animal's bioenergetics. We hypothesize that placozoans might have magnetoreception, which can be experimentally tested in future studies. In sum, <i>Trichoplax</i>, in particular, and Placozoa, in general, can be viewed as prospective reference species in traditional evolutionary and system biology but have the yet unexplored potential for planetary ecology and space biomedicine.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1514553"},"PeriodicalIF":4.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting heparan sulfate proteoglycans as an effective strategy for inhibiting cancer cell migration and invasiveness compared to heparin.","authors":"Lorenzo Depau, Jlenia Brunetti, Chiara Falciani, Elisabetta Mandarini, Marta Zanchi, Maria Francesca Paolocci, Marta Garfì, Alessandro Pini, Luisa Bracci","doi":"10.3389/fcell.2024.1505680","DOIUrl":"10.3389/fcell.2024.1505680","url":null,"abstract":"<p><p>By virtue of their ability to bind different growth factors, morphogens and extracellular matrix proteins, heparan sulfate proteoglycans (HSPGs) play a determinant role in cancer cell differentiation and migration. Despite a strong conceptual basis and promising preclinical results, clinical trials have failed to demonstrate any significant advantage of administering heparin to oncology patients. We exploited our anti-heparan sulfate branched peptide NT4 to test the opposite approach, namely, targeting HSPGs to interfere with their functions, instead of using heparin as a soluble competitor in human cell lines from pancreas adenocarcinoma, colon adenocarcinoma, rhabdomyosarcoma and two different breast cancers. We found that the anti-heparan sulfate peptide NT4 is more effective than heparin for inhibiting cancer cell adhesion, directional migration, colony formation and even cell growth, suggesting that targeting cell membrane HSPGs may be a more effective anti-metastatic strategy than using soluble heparin. Analysis of NT4 effects on cancer cell directional migration, associated to cellular distribution of HSPGs and cadherins in different migrating cancer cell lines, provided further indications on the molecular basis of HSPG functions, which may explain the efficiency of the HSPG targeting peptide.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1505680"},"PeriodicalIF":4.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of RBP-J interacting and tubulin-associated protein in the distribution of protein regulator of cytokinesis 1 in mitotic spindles.","authors":"Julia Caspers, Andreas Ritter, Badi Bahrami, Samira Catharina Hoock, Susanne Roth, Alexandra Friemel, Franz Oswald, Frank Louwen, Nina-Naomi Kreis, Juping Yuan","doi":"10.3389/fcell.2024.1472340","DOIUrl":"10.3389/fcell.2024.1472340","url":null,"abstract":"<p><p>The protein regulator of cytokinesis 1 (PRC1) is a key regulator of microtubule crosslinking and bundling, which is crucial for spindle formation and cytokinesis. RITA, the <i>R</i>BP-J <i>i</i>nteracting and <i>t</i>ubulin-<i>a</i>ssociated protein, is a microtubule associated protein. We have reported that RITA localizes to mitotic spindles modulating microtubule dynamics and stability as well as to spindle poles affecting the activity of Aurora A. As defective chromosome congression and segregation are the most remarkable features of cells depleted of RITA, we aimed to explore further potential related mechanisms, using various cellular and molecular techniques, including clustered regularly interspaced short palindromic repeats technique/deactivated CRISPR-associated protein 9 (CRISPR/dCas9), mass spectrometry, confocal microscopy, immunofluorescence, immunoprecipitation and Western blot analysis. Here, we show that FLAG-RITA precipitates PRC1 and tubulin, and that these two proteins co-localize in the central region of the central spindle. Reduction of RITA enlarges the staining area of PRC1 in mitotic spindles as well as in the central spindle. Its suppression reduces the inter-centromere distance in metaphase cells. Interestingly, microtubule bundles of the central spindle are often less organized in a non-parallel pattern, as evidenced by increased angles, relative to corresponding separating chromosomes. These data suggest a novel role for RITA in mitotic distribution of PRC1 and that its deregulation may contribute to defective chromosome movement during mitosis. As both RITA and PRC1 are closely associated with malignant progression, further work is required to elucidate the detailed molecular mechanisms by which RITA acts as a modulator in central spindle formation and cytokinesis.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"12 ","pages":"1472340"},"PeriodicalIF":4.6,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11747798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143003096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}