Frontiers in Cell and Developmental Biology最新文献

{"title":"Non-coding RNAs as key regulators of epithelial-mesenchymal transition in breast cancer.","authors":"Jing Peng, Wenhui Liu, Jiaju Tian, Yuncong Shu, Rui Zhao, Yuping Wang","doi":"10.3389/fcell.2025.1544310","DOIUrl":"https://doi.org/10.3389/fcell.2025.1544310","url":null,"abstract":"<p><p>This study examines the critical role of non-coding RNAs (ncRNAs) in regulating epithelial-mesenchymal transition (EMT) in breast cancer, a prevalent malignancy with significant metastatic potential. EMT, wherein cancer cells acquire mesenchymal traits, is fundamental to metastasis. ncRNAs-such as microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs)-modulate EMT by influencing gene expression and signaling pathways, affecting cancer cell migration and invasion. This review consolidates recent findings on ncRNA-mediated EMT regulation and explores their diagnostic and therapeutic potential. Specifically, miRNAs inhibit EMT-related transcription factors, while lncRNAs and circRNAs regulate gene expression through interactions with miRNAs, impacting EMT progression. Given the influence of ncRNAs on metastasis and therapeutic resistance, advancing ncRNA-based biomarkers and treatments holds promise for improving breast cancer outcomes.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1544310"},"PeriodicalIF":4.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11975958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143810884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendritic cell-derived MYD88 potentiates as a biomarker for immune regulation in hepatocellular carcinoma and may predict a better immunological result.","authors":"Zheming Liu, Hengbo Zhu, Fengxia Zhang, Wenting Huang, Shipeng Zhu, Songjiang He, Yi Yao, Qibin Song, Xue Zhang","doi":"10.3389/fcell.2025.1554705","DOIUrl":"10.3389/fcell.2025.1554705","url":null,"abstract":"<p><strong>Introduction: </strong>MYD88 (myeloid differentiation primary response 88) is a key adaptor protein mediate immune responses, primarily through Toll-like receptors (TLRs) and interleukin-1 receptor (IL-1R) signaling. The TLR/MYD88 pathway plays a critical role in dendritic cells (DC) maturation and function, contributing to the body's innate immunity. Recent studies have further highlighted MYD88's pivotal role in intrinsic immunity and its regulatory influence on the tumor microenvironment (TME) in hepatocellular carcinoma (HCC). The expression of MYD88 in DCs and its regulatory role in the TME have gained increasing attention.</p><p><strong>Methods: </strong>RNA-sequencing data retrieved from the TCGA and GEO databases were utilized for both the training and validation of our signature. Single-cell RNA transcriptome data from GEO were analyzed to investigate the correlation among subclusters of T cells, myeloid cells, and dendritic cells (DCs) within the HCC tumor microenvironment (TME). A combination of bioinformatics and machine learning approaches was employed to perform statistical analyses.Additionally, flow cytometry was conducted to quantify T cell subtypes and assess biomarker expression in DCs. A BALB/c-derived xenograft mouse model was established to evaluate the functional role of MyD88 in tumor progression and immunotherapy response. Furthermore, immunohistochemical (IHC) staining was performed to reassess the biological effects of MyD88 in HCC patients undergoing immune checkpoint inhibitor (ICI) therapy.</p><p><strong>Results: </strong>Our pan-cancer data analysis further highlights the significant impact of MYD88 on clinical outcomes in HCC. Analysis of TCGA and GEO databases confirms that MYD88 serves as a key signaling molecule in DCs, reinforcing its critical role in immune regulation. Our <i>in vitro</i> experiments demonstrates that MyD88 modulates T cell function through DCs. <i>In vivo</i>, H22 tumor cells exhibited accelerated growth in MyD88 knockout mice and a reduced response to anti-PD-1 treatment, whereas wild-type mice showed the opposite trend.</p><p><strong>Discussion: </strong>These findings underscore the critical role of MYD88 in DC function, suggesting its potential as a biomarker for immunoregulation in HCC. By shaping the TME, MYD88 not only regulates the immune response in HCC but also influences patient clinical outcomes. Both ex vivo and <i>in vivo</i> experiments further validate that MYD88 impacts DC functionality, contributing to variations in HCC progression.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1554705"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estrogen-induced FXR1 promotes endocrine resistance and bone metastasis in breast cancer via BCL2 and GPX4.","authors":"Yinzhong Shang, Tingfang Cao, Xin Ma, Le Huang, Mingming Wu, Junchao Xu, Jiarui Wang, Hao Wang, Sheng Wu, Vijay Pandey, Zhengsheng Wu, Weijie Zhang, Peter E Lobie, Xinghua Han, Tao Zhu","doi":"10.3389/fcell.2025.1563353","DOIUrl":"10.3389/fcell.2025.1563353","url":null,"abstract":"<p><p>Estrogen signaling dysregulation plays a critical role in the development of anti-estrogen resistance and bone metastasis of ER+ mammary carcinoma. Using quantitative proteomic screening, we identified FXR1 as an estrogen-regulated RNA-binding protein associated with anti-estrogen resistance. Mechanistically, estrogen and IGF1 facilitate FXR1 protein translation via the PI3K/AKT/mTOR/EIF4E pathway. FXR1 enhances cellular resistance to apoptosis and ferroptosis by facilitating the maturation of BCL2 pre-mRNA and stabilizing GPX4 mRNA, respectively. Anti-estrogen resistant cells exhibit elevated FXR1 expression, and FXR1 depletion restores their sensitivity to tamoxifen. Moreover, combining FXR1 depletion with a ferroptosis inducer induces synergistic lethal in anti-estrogen resistant cells. Finally, we provide proof-of-concept evidence supporting FXR1 antagonism as a potential treatment for bone metastases in ER+ breast cancer. Our findings highlight FXR1 as a promising therapeutic target to improve existing therapeutic regimes for ER+ breast cancer patients.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1563353"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of the Hippo/YAP pathway in the physiological activities and lesions of lens epithelial cells.","authors":"Shumei Tan, Xiaodan Jiang, Ziyuan Liu, Xuemin Li","doi":"10.3389/fcell.2025.1524814","DOIUrl":"10.3389/fcell.2025.1524814","url":null,"abstract":"<p><p>The Hippo/YAP pathway is a signaling pathway that plays an important role in cell proliferation, survival, differentiation, cell fate determination, organ size, and tissue homeostasis. Lens epithelial cells (LECs), located on the anterior surface of the lens, are the parental cells responsible for growth and development of the transparent ocular lens. During lens development, LECs undergo a process of differentiation where they exit the cell cycle and transform into lens fiber cells (LFCs), which constitute the majority of the lens structure. YAP is involved in the proliferation and differentiation of LECs, the maintenance of nuclear morphology, cell polarity, cell apical polarity complex, and connexin morphology. The role of the ordered arrangement of LFCs has been demonstrated in several animal studies, and <i>Yap1</i> heterozygous deletion mice exhibit cataracts. The mechanism of the Hippo/YAP pathway in the physiological activities and lesions of LECs is complex, which is of great significance to understanding the development of the lens and the pathogenesis of lens-related diseases.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1524814"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesangiogenic progenitor cells: a mesengenic and vasculogenic branch of hemopoiesis? A story of neglected plasticity.","authors":"Simone Pacini","doi":"10.3389/fcell.2025.1513440","DOIUrl":"10.3389/fcell.2025.1513440","url":null,"abstract":"<p><p>Mesangiogenic progenitor cells (MPCs) are mesengenic and vasculogenic cells isolated from human bone marrow mononuclear cell cultures. Although MPCs were first described over two decades ago and have demonstrated promising differentiation capabilities, these cells did not attract sufficient attention from experts in the field of tissue regeneration. Several reports from the first decade of the 2000s showed MPC-like cells co-isolated in primary mesenchymal stromal cell (MSC) cultures, applying human serum. However, in most cases, these rounded and firmly attached cells were described as \"contaminating\" cells of hemopoietic origin. Indeed, MPC morphology, phenotype, and functional features evoke but do not completely overlap with those of cultured peripheral macrophages, and their hemopoietic origin should not be excluded. The plasticity of cells from the monocyte lineage is surprising but not completely unprecedented. Underestimated data demonstrated that circulating monocyte/macrophages could acquire broader plasticity under specific and different culture conditions, and this plasticity could be a consequence of <i>in vitro</i> de-differentiation. The evidence discussed here suggests that MPCs could represent the cell identity toward which the de-differentiation process reprograms the circulating mature phagocytic compartment.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1513440"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome-mediated dual drug delivery of curcumin and methylene blue for enhanced cognitive function and mechanistic elucidation in Alzheimer's disease therapy.","authors":"Dongxin Yang, Zhuofen Deng, Hai Zhou, Qingshang Zhang, Xibing Zhang, Jun Gong","doi":"10.3389/fcell.2025.1562565","DOIUrl":"10.3389/fcell.2025.1562565","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is one of the neurodegenerative disorders, characterized by complex pathogenic mechanisms, including the deposition of beta-amyloid protein and hyperphosphorylation of Tau protein. There is currently a lack of effective therapeutic approaches for AD treatment. The aim of this study was to design exosomes (EXO) as a specifically designed carrier able to carry Curcumin (Cur) and Methylene Blue (MB) to improve cognitive function and to elucidate its underlying mechanisms. Our study results indicated that EXO-Cur+MB inhibited Tau protein phosphorylation by activating the AKT/GSK-3β pathway, while reversing cognitive dysfunction in AD mice by reducing apoptosis induced by okadaic acid (OA). Thus, our results suggested that EXO-Cur+MB would be of potential use for the treatment of AD.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1562565"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HTRecNet: a deep learning study for efficient and accurate diagnosis of hepatocellular carcinoma and cholangiocarcinoma.","authors":"Jingze Li, Yupeng Niu, Junwu Du, Jiani Wu, Weichen Guo, Yujie Wang, Jian Wang, Jiong Mu","doi":"10.3389/fcell.2025.1549811","DOIUrl":"10.3389/fcell.2025.1549811","url":null,"abstract":"<p><strong>Background: </strong>Hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) represent the primary liver cancer types. Traditional diagnostic techniques, reliant on radiologist interpretation, are both time-intensive and often inadequate for detecting the less prevalent CCA. There is an emergent need to explore automated diagnostic methods using deep learning to address these challenges.</p><p><strong>Methods: </strong>This study introduces HTRecNet, a novel deep learning framework for enhanced diagnostic precision and efficiency. The model incorporates sophisticated data augmentation strategies to optimize feature extraction, ensuring robust performance even with constrained sample sizes. A comprehensive dataset of 5,432 histopathological images was divided into 5,096 for training and validation, and 336 for external testing. Evaluation was conducted using five-fold cross-validation and external validation, applying metrics such as accuracy, area under the receiver operating characteristic curve (AUC), and Matthews correlation coefficient (MCC) against established clinical benchmarks.</p><p><strong>Results: </strong>The training and validation cohorts comprised 1,536 images of normal liver tissue, 3,380 of HCC, and 180 of CCA. HTRecNet showed exceptional efficacy, consistently achieving AUC values over 0.99 across all categories. In external testing, the model reached an accuracy of 0.97 and an MCC of 0.95, affirming its reliability in distinguishing between normal, HCC, and CCA tissues.</p><p><strong>Conclusion: </strong>HTRecNet markedly enhances the capability for early and accurate differentiation of HCC and CCA from normal liver tissues. Its high diagnostic accuracy and efficiency position it as an invaluable tool in clinical settings, potentially transforming liver cancer diagnostic protocols. This system offers substantial support for refining diagnostic workflows in healthcare environments focused on liver malignancies.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1549811"},"PeriodicalIF":4.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143802876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferroptosis in neurodegenerative diseases: mechanisms and therapeutic potential of stem cell derivatives.","authors":"Ting Zhang, Yusu Zhang, Jinpeng Xie, Dandan Lu, Lihong Wang, Shuaifei Zhao, Jing Zhou, Yang Cheng, Ting Kou, Jue Wang, Ying Chen, Lei Xu, Xiangyu Hu, Yuxiu Ying, Jun Wang, Xiaoshuang Xin, Xu Xu, Siyun Lei, Chenyu Qiu, Jinhua Wu, Qiqi Lyu, Tong Cao","doi":"10.3389/fcell.2025.1577382","DOIUrl":"10.3389/fcell.2025.1577382","url":null,"abstract":"<p><p>Ferroptosis, a non-apoptotic, iron-dependent form of regulated cell death, is closely related to the pathogenesis of neurodegenerative diseases. Stem cells and their derivatives exhibit remarkable potential in modulating ferroptosis, offering promising therapeutic intervention for neurodegenerative diseases. In this review, we systematically explore neurological aging and its association with cognitive impairment and neurodegenerative diseases, with focus on the molecular mechanisms of ferroptosis in neurodegenerative diseases and the potential therapeutic strategies of stem cell derivatives for neurological diseases.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1577382"},"PeriodicalIF":4.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11968680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison between manual and automated methods for the isolation of mononuclear cells and mesenchymal stem cells using ficoll: efficacy and reproducibility.","authors":"Ester Moñivas, Concepción Aguayo, Silvia de la Calle, Paula Martínez, Nieves Repollés, Mercedes Zurita","doi":"10.3389/fcell.2025.1556697","DOIUrl":"10.3389/fcell.2025.1556697","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs), hold immense therapeutic promise in Advanced Therapy Medicinal Products (ATMPs) due to their multipotent nature, immunomodulatory properties, and anti-inflammatory effects. However, a significant challenge lies in obtaining sufficient quantities of MSCs for therapeutic applications, necessitating <i>ex-vivo</i> culture of Mononuclear Cells (MNCs) isolated from source tissues like bone marrow. This study compares the efficacy of MNC isolation using manual and automated methods, specifically evaluating the Sepax system, and investigates whether the isolation method impacts MSCs yield. Seventeen bone marrow samples were processed using both methods, with subsequent analysis of MNC and colony-forming unit (CFU) counts, MSCs differentiation potential, and phenotypic characterization. While the Sepax system demonstrated slightly higher MNC yields, no significant differences were observed in CFU formation or MSCs characteristics compared to manual isolation. These findings underscore the importance of critically evaluating isolation methods to ensure both efficiency and quality in therapeutic applications.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1556697"},"PeriodicalIF":4.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11969043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thalassemia and assisted reproduction: non-transfusion-dependent thalassemia shows no significant effect on live birth rates after embryo transfer.","authors":"Li Fan, Liuyan Wei, Ni Tang, Zhetao Li, Wugao Li, Liuying Nong, Jingjing Li, Wenjie Huang","doi":"10.3389/fcell.2025.1573572","DOIUrl":"10.3389/fcell.2025.1573572","url":null,"abstract":"<p><strong>Background: </strong>Thalassemia is a hereditary blood disorder that can impact fertility due to various factors such as iron overload and endocrine disruption. While the effects of iron overload on fertility outcomes in transfusion-dependent thalassemia (TDT) have been well-documented, there is limited data on how NTDT affects assisted reproductive technology (ART) outcomes. This study aims to assess the fertility and pregnancy outcomes of NTDT patients compared to thalassemia carriers (TC) patients in IVF and frozen embryo transfer (FET) cycles.</p><p><strong>Methods: </strong>This retrospective cohort study analyzed 6,911 female patients who underwent autologous IVF treatment at a private reproductive center between January 2013 and December 2022. The study included women who were carriers of thalassemia or diagnosed with NTDT. ART outcomes, including oocyte retrieval rate, embryo development (maturation rate, number of fertilized oocytes and blastocyst formation rate), clinical pregnancy rate, live birth rate, and miscarriage rate, were compared between NTDT and TC patients. Propensity score matching (PSM) and multivariable adjustments for potential confounders were applied in the statistical analyses.</p><p><strong>Results: </strong>NTDT patients had a significantly lower oocyte retrieval rate (0.88 vs. 0.93, p < 0.05) and a longer interval from medication initiation to oocyte retrieval (13.35 days vs. 12.38 days, p < 0.05) compared to TC patients. However, NTDT patients exhibited higher oocyte maturation rates and a greater number of fertilized oocytes. Despite these differences in embryo development metrics, there were no statistically significant differences in clinical pregnancy rates and live birth rates between NTDT and TC patients in both fresh embryo transfer (IVF-ET) and FET cycles (p > 0.05). These findings suggest that while NTDT may affect certain aspects of embryo development, it does not significantly impact overall pregnancy outcomes in ART.</p><p><strong>Conclusion: </strong>This study provides valuable insights into ART outcomes for NTDT patients, showing that, despite challenges in oocyte retrieval, their fertility and pregnancy outcomes are comparable to those of thalassemia carriers. Clinicians should consider individualized treatment plans and provide comprehensive counseling for NTDT patients, focusing on their specific fertility characteristics, to optimize ART outcomes. Further research is needed to explore the underlying mechanisms affecting embryo development in NTDT patients and to confirm these findings in broader populations.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1573572"},"PeriodicalIF":4.6,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11968656/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}