Frontiers in Cell and Developmental Biology最新文献

{"title":"Sodium butyrate and sodium propionate inhibit breast cancer cell migration and invasion through regulation of epithelial-to-mesenchymal transition and suppression of MEK/ERK signaling pathway.","authors":"Dania Mahmoud Kharazi, Louna Karam, Charbel El Boustany, José-Noel Ibrahim","doi":"10.3389/fcell.2025.1535563","DOIUrl":"10.3389/fcell.2025.1535563","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the roles played by NaB and NaP in breast carcinogenesis by elucidating their potential anti-metastatic effects in the context of tumor migration, invasion, and EMT regulation in two distinct breast cancer cell lines, MCF-7 and MDA-MB-231.</p><p><strong>Methods: </strong>The cytotoxic effect of both compounds on 3D spheroid formation was evaluated using a hanging drop assay. The anti-migratory and anti-invasive potentials of NaB and NaP were investigated through transwell migration and invasion assays. Moreover, their role in regulating epithelial-to-mesenchymal transition (EMT) was examined by assessing E-cadherin, vimentin, and β-catenin mRNA and protein expression levels through RT-qPCR and Western blot or flow cytometry. β-Catenin localization upon treatment was further visualized via immunofluorescence. Protein expression of MEK, p-MEK, ERK, and p-ERK was analyzed by Western blot.</p><p><strong>Results: </strong>Our results revealed a dose- and time-dependent impairment of spheroid formation in both cell lines, with NaB exerting a more potent effect than NaP. Both SCFAs were able to significantly inhibit migration and invasion of MDA-MB-231 cells following 24 h of treatment. Moreover, treatment with NaB or NaP altered the mRNA and protein profile of EMT-associated markers and abrogated the nuclear translocation of β-catenin. Finally, ERK and MEK phosphorylation was reduced in MDA-MB-231 and MCF-7 cells upon treatment with NaB, and less prominently with NaP.</p><p><strong>Conclusion: </strong>Our study highlights the promising therapeutic potential of NaB and NaP, providing insight into their inhibitory effects on 3D formation, migration, and invasion through EMT regulation and deactivation of MEK/ERK signaling in breast cancer.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1535563"},"PeriodicalIF":4.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of HSC development and function by Lin28b.","authors":"Grant Cox, Michihiro Kobayashi, Brian D Rudd, Momoko Yoshimoto","doi":"10.3389/fcell.2025.1555877","DOIUrl":"10.3389/fcell.2025.1555877","url":null,"abstract":"<p><p>Hematopoietic stem cells (HSCs) provide all kinds of blood cells for life while maintaining self-renewal ability. During development, HSCs are first produced in the mouse embryo around embryonic day (E) 11. At this time, only one or two transplantable HSCs can be detected per embryo. Then, HSCs migrate to the fetal liver, where the number of HSCs rapidly increases, showing enhanced self-renewal ability. After birth, a transition occurs from the rapidly proliferating fetal HSCs to the more slowly dividing adult HSCs, which ends by 3-4 weeks of age. It is known that fetal HSCs express distinct surface markers and transcriptomes and produce a variety of distinct immune cells that are not made by adult HSCs. Accumulating evidence indicates that the ontogeny of the hematopoietic system is driven by a highly conserved and developmentally regulated RNA binding protein known as <i>Lin28b</i>. <i>Lin28b</i> is predominantly expressed in the fetal hematopoietic stem and progenitor cells (HSPCs) and regulates the developmental switch from fetal to adult HSCs. In this review, we will provide an overview of how <i>Lin28b</i> regulates the expansion and differentiation of HSCs in early life. These insights can be taken into consideration when developing <i>ex vivo</i> HSC expansion utilizing such physiological characteristics of HSCs.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1555877"},"PeriodicalIF":4.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Action and therapeutic targets of folliculin interacting protein 1: a novel signaling mechanism in redox regulation.","authors":"Qingzhi Ran, Aoshuang Li, Bo Yao, Chunrong Xiang, Chunyi Qu, Yongkang Zhang, Xuanhui He, Hengwen Chen","doi":"10.3389/fcell.2025.1523489","DOIUrl":"10.3389/fcell.2025.1523489","url":null,"abstract":"<p><p>Rapid activation of adenosine monophosphate-activated protein kinase (AMPK) induces phosphorylation of mitochondrial-associated proteins, a process by which phosphate groups are added to regulate mitochondrial function, thereby modulating mitochondrial energy metabolism, triggering an acute metabolic response, and sustaining metabolic adaptation through transcriptional regulation. AMPK directly phosphorylates folliculin interacting protein 1 (FNIP1), leading to the nuclear translocation of transcription factor EB (TFEB) in response to mitochondrial functions. While mitochondrial function is tightly linked to finely-tuned energy-sensing mobility, FNIP1 plays critical roles in glucose transport and sensing, mitochondrial autophagy, cellular stress response, and muscle fiber contraction. Consequently, FNIP1 emerges as a promising novel target for addressing aberrant mitochondrial energy metabolism. Recent evidence indicates that FNIP1 is implicated in mitochondrial biology through various pathways, including AMPK, mTOR, and ubiquitination, which regulate mitochondrial autophagy, oxidative stress responses, and skeletal muscle contraction. Nonetheless, there is a dearth of literature discussing the physiological mechanism of action of FNIP1 as a novel therapeutic target. This review outlines how FNIP1 regulates metabolic-related signaling pathways and enzyme activities, such as modulating mitochondrial energy metabolism, catalytic activity of metabolic enzymes, and the homeostasis of metabolic products, thereby controlling cellular function and fate in different contexts. Our focus will be on elucidating how these metabolite-mediated signaling pathways regulate physiological processes and inflammatory diseases.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1523489"},"PeriodicalIF":4.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerebrospinal fluid-contacting neurons: a promising source for adult neural stem cell transplantation in spinal cord injury treatment.","authors":"Zhangrong Luo, Zeyu Shangguan, Liang Cao, Yi Zhang, Qizhe Li, Xuexing Shi, Jiangquan Fu, Chunqing Wang, Xiaowei Dou, Wei Tan, Qing Li","doi":"10.3389/fcell.2025.1549194","DOIUrl":"10.3389/fcell.2025.1549194","url":null,"abstract":"<p><p>Transplantation of adult neural stem cells (NSCs) is regarded as one of the most promising approaches for treating spinal cord injury (SCI). However, securing a sufficient and reliable source of adult NSCs remains one of the primary challenges in applying this method for SCI treatment. Cerebrospinal fluid-contacting neurons (CSF-cNs) act as adult NSCs and can be substantially expanded <i>in vitro</i> while maintaining their NSC characteristics even after 60 passages. When CSF-cNs are transplanted into the injury sites of SCI mice, they demonstrate high survival rates along with the ability to proliferate and differentiate into neurons, astrocytes, and oligodendrocytes. Additionally, significant improvements in motor function have been observed in SCI mice following the transplantation of CSF-cNs. These results suggest that CSF-cNs may represent a promising source of adult NSCs for transplantation therapy in SCI.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1549194"},"PeriodicalIF":4.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functions of <i>Drosophila</i> Toll/NF-κB signaling in imaginal tissue homeostasis and cancer.","authors":"Fabienne Brutscher, Konrad Basler","doi":"10.3389/fcell.2025.1559753","DOIUrl":"10.3389/fcell.2025.1559753","url":null,"abstract":"<p><p>The Toll/NF-κB pathway plays a central role in patterning the <i>Drosophila</i> embryo and in orchestrating the innate immune response against microbial infections. Both discoveries were associated with a Nobel Prize award and led to the recognition of the Toll-like receptor pathway in mammals, which has significant implications for diseases. Recent discoveries have revealed that the Toll/NF-κB pathway also maintains epithelial homeostasis of imaginal tissues during development: local Toll/NF-κB signaling activity monitors internal cellular fitness, and precancerous mutant cells can trigger systemic Toll/NF-κB pathway activation. However, this signaling can be exploited in diseases like cancer, in which Toll/NF-κB signaling is often co-opted or subverted. Various models have been proposed to explain how Toll/NF-κB signaling contributes to different types of cancer. Here we provide an overview of the functions of Toll/NF-κB signaling in imaginal tissue homeostasis with a focus on their misuse in pathological contexts, particularly their significance for tumor formation.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1559753"},"PeriodicalIF":4.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143718407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Speed vs completeness: a comparative study of solitary and colonial tunicate embryogenesis.","authors":"Chiara Anselmi, Katherine J Ishizuka, Karla J Palmeri, Paolo Burighel, Ayelet Voskoboynik, Kohji Hotta, Lucia Manni","doi":"10.3389/fcell.2025.1540212","DOIUrl":"10.3389/fcell.2025.1540212","url":null,"abstract":"<p><p>Solitary ascidians, such as <i>Ciona robusta</i>, have been used for over a century as model systems for embryological studies. These species are oviparous, producing many relatively small and transparent eggs, which are released and fertilized outside the parent body. Embryos develop rapidly in a stereotyped manner and reach the larva stage in less than 1 day (at 20°C). The larvae then settle and metamorphose into sessile juveniles in approximately 2 days. On the other hand, colonial ascidians are ovoviviparous, with heavily yolked eggs that develop inside the parent body. In the colonial <i>Botryllus schlosseri</i>, embryos are connected to the parental body via a cup-like placenta and develop into larvae within a week (at 20°C). These larvae, which possess both typical larval organs and prospective juvenile organs, are released into seawater, where they settle very rapidly, sometimes after only 15 minutes of free swimming. Then, they metamorphose into juvenile oozooids. The ability to study embryo development in colonial ascidians within the parent body is limited. To address this, we developed a method for <i>in vitro</i> culturing <i>B. schlosseri</i> embryos outside the parental body and combined it with time-lapse and confocal microscopy to describe the embryonic developmental stages. Moreover, we used histological analysis based on serial sections to investigate late-stage development, when embryo opacity made other techniques ineffective. We identified 19 stages of development, from the fertilized egg to the swimming larva, and described the stage of organ appearance and differentiation. Comparing the embryonic development timeline of <i>B. schlosseri</i> with that of <i>C. robusta</i>, we found heterochrony in development, particularly in the timing of organ appearance and growth rate. We hypothesize that this difference in maturation timing between solitary and colonial ascidians reflects a shift in the regulation of key developmental pathways that contributed to ascidian diversification. This heterochronic evolution likely facilitated a significant (approximately four-fold) shortening of the metamorphosis time in <i>B. schlosseri</i> by allowing embryos to remain in a safe ovoviviparous environment five times longer than those in <i>C. robusta</i> before hatching.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1540212"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lysosomes' fallback strategies: more than just survival or death.","authors":"Quan Wang, Ruolin Wang, Haihui Hu, Xiaoqing Huo, Fulong Wang","doi":"10.3389/fcell.2025.1559504","DOIUrl":"10.3389/fcell.2025.1559504","url":null,"abstract":"<p><p>Lysosomes are heterogeneous, acidic organelles whose proper functionality is critically dependent on maintaining the integrity of their membranes and the acidity within their lumen. When subjected to stress, the lysosomal membrane can become permeabilized, posing a significant risk to the organelle's survival and necessitating prompt repair. Although numerous mechanisms for lysosomal repair have been identified in recent years, the progression of lysosome-related diseases is more closely linked to the organelle's alternative strategies when repair mechanisms fail, particularly in the contexts of aging and pathogen infection. This review explores lysosomal responses to damage, including the secretion of lysosomal contents and the interactions with lysosome-associated organelles in the endolysosomal system. Furthermore, it examines the role of organelles outside this system, such as the endoplasmic reticulum (ER) and Golgi apparatus, as auxiliary organelles of the endolysosomal system. These alternative strategies are crucial to understanding disease progression. For instance, the secretion and spread of misfolded proteins play key roles in neurodegenerative disease advancement, while pathogen escape via lysosomal secretion and lysosomotropic drug expulsion underlie cancer treatment resistance. Reexamining these lysosomal fallback strategies could provide new perspectives on lysosomal biology and their contribution to disease progression.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1559504"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the study of ARR3 in myopia.","authors":"Yi-Ming Guo, Junhan Wei, Jiaqi Wang, Guoyun Zhang, Jiejing Bi, Lu Ye","doi":"10.3389/fcell.2025.1551135","DOIUrl":"10.3389/fcell.2025.1551135","url":null,"abstract":"<p><p>The ARR3 gene (cone arrestin, OMIM: 301770) has gained significant attention as a pivotal factor in the etiology of myopia, particularly early-onset high myopia (eoHM). As a member of the arrestin gene family, ARR3 is predominantly expressed in cone photoreceptors, playing a crucial role in visual processing. Recent studies have identified specific mutations in ARR3 that correlate with an elevated risk of myopia development, highlighting its potential involvement in the disease's pathogenesis. This review summarizes current advancements in elucidating the relationship between ARR3 and myopia, emphasizing genetic variations associated with refractive errors and their implications for myopia research and clinical management. We emphasize the necessity for further studies to elucidate the role of ARR3 in myopia, particularly regarding its impact on visual development and the genetic predisposition observed in specific populations.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1551135"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discrepancies between human and murine model cerebral aneurysms at single-cell resolution.","authors":"Hang Ji, Guicheng Kuang, Hailan Yang, Haitao Liu, Yue Li, Shaoshan Hu, Anqi Xiao, Chao You, Haogeng Sun, Chaofeng Fan, Guozhang Sun","doi":"10.3389/fcell.2025.1512938","DOIUrl":"10.3389/fcell.2025.1512938","url":null,"abstract":"<p><strong>Background: </strong>The murine model of cerebral aneurysm (CA) serves as a prevalent tool for investigating the molecular underpinnings of CA. However, the extent to which the CA murine model aligns with that of human remains elusive.</p><p><strong>Methods: </strong>The present study employed a comprehensive integration and exploration of the single-cell RNA-seq (scRNA-seq) datasets, along with multiple trajectory and gene regulatory network analyses, to investigate the cellular and molecular discrepancies between human and murine model CAs.</p><p><strong>Results: </strong>The uniform manifold approximation and projection (umap) embedding exhibits that the primary discrepancies between human and murine model CAs reside in the cells of modifiable phenotype, encompassing vascular smooth muscle cell (vSMC), monocyte/macrophage, and neutrophil. The vSMCs from human CA tissue exhibit a fibroblast-like phenotype in comparison to that of murine model. Distinct patterns of neutrophil recruitment are observed in human and murine models, with the former characterized by neutrophil-derived CXCL8 and the latter by monocyte/macrophage-derived CCLs. In addition, macrophages originated from human unruptured CA express higher levels of M2 gene markers. Moreover, the inflammatory status of the CA tissue differs between humans and mouse models, with the former exhibiting a more acute and intense inflammation.</p><p><strong>Conclusion: </strong>These findings demonstrate subtle but important disparities between human and murine model CAs, and may shed light upon an optimization of murine CA model.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1512938"},"PeriodicalIF":4.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11933115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship mammalian p38 with human health and its homolog Hog1 in response to environmental stresses in <i>Saccharomyces cerevisiae</i>.","authors":"Gang Du, Kaifang Zheng, Cunying Sun, Mingyue Sun, Jie Pan, Dan Meng, Wenqiang Guan, Hui Zhao","doi":"10.3389/fcell.2025.1522294","DOIUrl":"10.3389/fcell.2025.1522294","url":null,"abstract":"<p><p>The mammalian p38 MAPK pathway plays a vital role in transducing extracellular environmental stresses into numerous intracellular biological processes. The p38 MAPK have been linked to a variety of cellular processes including inflammation, cell cycle, apoptosis, development and tumorigenesis in specific cell types. The p38 MAPK pathway has been implicated in the development of many human diseases and become a target for treatment of cancer. Although MAPK p38 pathway has been extensively studied, many questions still await clarification. More comprehensive understanding of the MAPK p38 pathway will provide new possibilities for the treatment of human diseases. Hog1 in <i>S. cerevisiae</i> is the conserved homolog of p38 in mammalian cells and the HOG MAPK signaling pathway in <i>S. cerevisiae</i> has been extensively studied. The deep understanding of HOG MAPK signaling pathway will help provide clues for clarifying the p38 signaling pathway, thereby furthering our understanding of the relationship between p38 and disease. In this review, we elaborate the functions of p38 and the relationship between p38 and human disease. while also analyzing how Hog1 regulates cellular processes in response to environmental stresses. 1, p38 in response to various stresses in mammalian cells.2, The functions of mammalian p38 in human health.3, Hog1 as conserved homolog of p38 in response to environmental stresses in <i>Saccharomyces cerevisiae</i>. 1, p38 in response to various stresses in mammalian cells. 2, The functions of mammalian p38 in human health. 3, Hog1 as conserved homolog of p38 in response to environmental stresses in <i>S. cerevisiae</i>.</p>","PeriodicalId":12448,"journal":{"name":"Frontiers in Cell and Developmental Biology","volume":"13 ","pages":"1522294"},"PeriodicalIF":4.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}