Frontiers in Dentistry最新文献

Bonding to Molar-Incisor Hypomineralization Affected Teeth: A Scoping Review of Adhesive Strategies, In Vitro Performance, and Clinical Outcomes. 磨牙-门牙低矿化影响牙齿的粘接：粘接策略、体外性能和临床结果的综述。

Frontiers in Dentistry Pub Date : 2026-04-05 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i16.21619

Fatemeh Mahmoodi Khorandi, Shayan Moradi, Golnaz Tayebi, Tabassom Hooshmand

{"title":"Bonding to Molar-Incisor Hypomineralization Affected Teeth: A Scoping Review of Adhesive Strategies, In Vitro Performance, and Clinical Outcomes.","authors":"Fatemeh Mahmoodi Khorandi, Shayan Moradi, Golnaz Tayebi, Tabassom Hooshmand","doi":"10.18502/fid.v23i16.21619","DOIUrl":"10.18502/fid.v23i16.21619","url":null,"abstract":"Molarincisor hypomineralization (MIH) poses major restorative challenges due to compromised enamel and its reduced bonding capacity. This scoping review evaluated treatment options for MIHaffected young permanent teeth following PRISMAScR guidelines. A systematic search in PubMed, Cochrane, Science Direct and gray literature identified 21 eligible studies published between 2013 and 2025, including invitro and clinical studies. Laboratory findings demonstrated lower bond strengths in MIH enamel compared with the sound enamel. Etchandrinse adhesives performed more reliably than that of selfetch systems, while pretreatment procedures improved adhesion in some settings. Clinical studies showed resinbased sealants and composites bonded with etchandrinse adhesives achieved higher retention. Glass ionomer and glass hybrid restorations offered mostly short-term success and are best used as an interim option. Investigated indirect restorations provided superior longevity in severe cases. Preventive adjuncts such as applying varnishes reduced hypersensitivity and caries risk. Finally, this review highlights the central role of adhesive performance in MIH management.","PeriodicalId":12445,"journal":{"name":"Frontiers in Dentistry","volume":"23 ","pages":"16"},"PeriodicalIF":0.0,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaccine Design against Fusobacterium nucleatum FadA Protein in Periodontal Infections. 牙周感染核梭杆菌FadA蛋白疫苗设计

Frontiers in Dentistry Pub Date : 2026-04-01 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i15.21618

Neha N Rathod, M Jeevitha, Pradeep Kumar Yadalam, Amir Raee, Artak Heboyan

{"title":"Vaccine Design against Fusobacterium nucleatum FadA Protein in Periodontal Infections.","authors":"Neha N Rathod, M Jeevitha, Pradeep Kumar Yadalam, Amir Raee, Artak Heboyan","doi":"10.18502/fid.v23i15.21618","DOIUrl":"10.18502/fid.v23i15.21618","url":null,"abstract":"Objectives: A common inflammatory condition affecting oral health is periodontitis, which can lead to tooth loss if not treated early, as it destroys the supporting structures of the teeth. Fusobacterium nucleatum is an orange-pigmented bacterium commonly referred to as \"the bridging species\" between early and late colonizers in the oral microbiota, and it is associated with the progression of periodontal disease. This study aimed to develop an effective and precise multi-epitope vaccine against F. nucleatum, as there is currently no approved vaccine for periodontal disease. Materials and Methods: This study employed a computational method to design a multiple epitope-based vaccine against F. nucleatum using an immunoinformatics reverse vaccinology approach. FadA, a novel adhesin unique to F. nucleatum, was a suitable peptide for vaccine development. B- and T-cell epitopes were identified and assessed to design a multi-epitope vaccine. The developed vaccine construct was analyzed for potency based on its allergenicity, antigenicity, solubility, and stability. Results: The vaccine structure was non-allergenic and exhibited high antigenicity, stability, and solubility. This vaccine may be a candidate for eradicating F. nucleatum, based on predictions of immunogenicity responses. Conclusion: The designed vaccine construct could generate an effective immune response against F. nucleatum, owing to its high antigenicity and non-allergenicity.","PeriodicalId":12445,"journal":{"name":"Frontiers in Dentistry","volume":"23 ","pages":"15"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early Prediction of Oral Precancerous Lesions Using Artificial Intelligence. 人工智能在口腔癌前病变早期预测中的应用。

Frontiers in Dentistry Pub Date : 2026-03-20 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i14.21568

Pradeep Kumar Rathinavelu, Vyshali Pillai, Pradeep Kumar Yadalam, Sri Shivasankari Thilagar, Amir Raee, Artak Heboyan

{"title":"Early Prediction of Oral Precancerous Lesions Using Artificial Intelligence.","authors":"Pradeep Kumar Rathinavelu, Vyshali Pillai, Pradeep Kumar Yadalam, Sri Shivasankari Thilagar, Amir Raee, Artak Heboyan","doi":"10.18502/fid.v23i14.21568","DOIUrl":"10.18502/fid.v23i14.21568","url":null,"abstract":"Objectives: Oral potentially malignant disorders may present as white, red, or mixed red-white lesions and require accurate early recognition. This study evaluated whether texture-analysis features extracted from clinical digital images could distinguish oral precancerous lesions from other oral mucosal lesions and normal mucosa using gray-level co-occurrence matrix (GLCM), gray-level run-length matrix (GLRLM), and wavelet analysis. Materials and Methods: Sixty-four clinical digital images were selected according to predefined inclusion and exclusion criteria. The dataset included leukoplakia, erythroplakia, oral submucous fibrosis, candidiasis, lichen planus, leukoderma, frictional keratosis, white spongy nevus, and normal mucosa. Regions of interest were extracted from each image, and texture features were derived using GLCM, GLRLM, and wavelet analysis. A support vector machine (SVM) classifier was then used to categorize images as oral precancerous lesions or non-precancerous/normal mucosa. Results: GLCM yielded the highest classification accuracy (88%), followed by GLRLM (81%) and wavelet analysis (79%). The corresponding sensitivity values were 77%, 64%, and 60%, and the specificity values were 93%, 90%, and 89%, respectively. The positive predictive values were 83% for GLCM, 75% for GLRLM, and 75% for wavelet analysis. Conclusion: GLCM-based texture features provided the best diagnostic performance in this dataset. These image-analysis methods may be useful as non-invasive adjuncts to conventional clinical examination and histopathological diagnosis; however, larger datasets and external validation are required before clinical implementation.","PeriodicalId":12445,"journal":{"name":"Frontiers in Dentistry","volume":"23 ","pages":"14"},"PeriodicalIF":0.0,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148663717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outcome of Guided Pulpal Regeneration in Immature Molar Teeth with Chronic Apical Abscess: A Case Report and Mini-Review. 未成熟磨牙慢性根尖脓肿引导下牙髓再生1例报告及回顾。

Frontiers in Dentistry Pub Date : 2026-03-14 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i13.21567

Mohammad Mahdi Kiafar, Farzaneh Afkhami, Sholeh Ghabraei, Ove A Peters, Chun Xu, Seyyed Ali Abaee

引用次数: 0

Effect of Listerine and Sodium Fluoride Mouthwashes on Force Decay of Orthodontic Memory Elastomeric Chains: An In Vitro Study. 李斯德林和氟化钠漱口水对正畸记忆弹性链力衰减影响的体外研究

Frontiers in Dentistry Pub Date : 2026-03-05 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i12.21558

Sara Alijani, Jamshid Nemati, Mozhde Borjali, Aysa Nezakatmaab, Behnaz Alafchi

{"title":"Effect of Listerine and Sodium Fluoride Mouthwashes on Force Decay of Orthodontic Memory Elastomeric Chains: An In Vitro Study.","authors":"Sara Alijani, Jamshid Nemati, Mozhde Borjali, Aysa Nezakatmaab, Behnaz Alafchi","doi":"10.18502/fid.v23i12.21558","DOIUrl":"10.18502/fid.v23i12.21558","url":null,"abstract":"Objectives: The interaction of mouthwashes with orthodontic elastomeric materials may alter their mechanical properties. The main purpose of this study was to evaluate the impact of 0.05% sodium fluoride Oral-B PRO-Expert Multi Protection and fluoride-free Listerine Advanced with Tartar Protection mouthwashes on the force decay behavior of memory elastomeric chains. Materials and Methods: In this in vitro study, 90 pieces of closed gray memory elastomeric chains were randomly allocated to three distinct groups (n=30): control, sodium fluoride, and Listerine. The control samples remained fully submerged in artificial saliva over the course of the experiment. Test samples were placed daily for 1 minute in either sodium fluoride or Listerine mouthwash, then in a solution consisting of equal parts of mouthwash and artificial saliva for 30 minutes, followed by a thorough rinse with distilled water, and then placed back in the artificial saliva. Tensile tests were conducted at various time points (baseline, 1 hour, and 1, 7, and 28 days) (n=6). Data were analyzed by one-way and two-way ANOVA, and Tukey's post-hoc test (alpha=0.05). Results: All groups exhibited a continuous force decay over time (P<0.001). The experimental groups demonstrated significantly greater force decay compared to the control group (P<0.001). The sodium fluoride group exhibited significantly greater force decay compared to the Listerine group at all measured time points (P<0.001) except on day 28 (P=0.970). Conclusion: Both mouthwashes accelerated the force degradation of memory elastomeric chains, with sodium fluoride causing a greater reduction except on day 28.","PeriodicalId":12445,"journal":{"name":"Frontiers in Dentistry","volume":"23 ","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Two Bleaching Techniques on Teeth Discolored with Different Calcium Silicate-Based Cements. 两种漂白方法对不同硅酸钙基骨水泥牙体变色的影响。

Frontiers in Dentistry Pub Date : 2026-03-02 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i11.21496

Somayeh Hekmatfar, Vahid Samadi, Avissasadat Meraji, Karim Jafari

{"title":"Effect of Two Bleaching Techniques on Teeth Discolored with Different Calcium Silicate-Based Cements.","authors":"Somayeh Hekmatfar, Vahid Samadi, Avissasadat Meraji, Karim Jafari","doi":"10.18502/fid.v23i11.21496","DOIUrl":"10.18502/fid.v23i11.21496","url":null,"abstract":"Objectives: The objective of this study was to investigate tooth discoloration caused by different calcium silicate-based cements (CSCs), and assess the efficacy of two bleaching techniques in restoring the original tooth color. Materials and Methods: In this in vitro study, 54 sound teeth were divided into three groups (n=18) of Endocem, MTA Angelus, and calcium-enriched mixture (CEM) cement. Baseline color assessments were conducted using a spectrophotometer, with subsequent color evaluation after 3 months. Each group was divided into two subgroups of internal bleaching and internal/external bleaching. The bleaching procedure was performed over a 2-week period. Two-way ANOVA was applied to investigate the interaction effect of bleaching technique and time on color change (∆E). The Tukey's test was used as post-hoc for pairwise comparisons. P≤0.05 was considered significant. Results: The results indicated noticeable color change in all test groups, surpassing the perceptibility threshold (ΔE>3.3). Endocem exhibited the maximum discoloration, while CEM cement displayed the minimum. Significant differences were observed between Endocem and other groups (P<0.05). Regarding the L*, a*, and b* parameters, no significant differences were found between the two subgroups except in Endocem group, where internal/external bleaching caused a significantly greater increase in color parameters compared to internal bleaching (P=0.001). Conclusion: Endocem induced more discoloration than MTA Angelus and CEM cement. However, there was no difference in efficacy between the internal and internal/external bleaching techniques (except in Endocem), both proving effective in restoring the original color of discolored teeth.","PeriodicalId":12445,"journal":{"name":"Frontiers in Dentistry","volume":"23 ","pages":"11"},"PeriodicalIF":0.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Enamel Roughness and Calcium Release between Manual and Mechanical Stripping with and without Polishing after Demineralization. 脱矿后手工剥离与机械剥离牙釉质粗糙度及钙释放量的比较。

Frontiers in Dentistry Pub Date : 2026-02-25 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i10.21495

Negar Asgarian, Elham Ahmadi, Farzaneh Sadeghi Mahounak, Mohammad Javad Kharrazi Fard, Mohammad Sadegh Ahmad Akhoundi

{"title":"Comparison of Enamel Roughness and Calcium Release between Manual and Mechanical Stripping with and without Polishing after Demineralization.","authors":"Negar Asgarian, Elham Ahmadi, Farzaneh Sadeghi Mahounak, Mohammad Javad Kharrazi Fard, Mohammad Sadegh Ahmad Akhoundi","doi":"10.18502/fid.v23i10.21495","DOIUrl":"10.18502/fid.v23i10.21495","url":null,"abstract":"Objectives: Proximal enamel stripping is one way to create space in orthodontic treatments that leaves grooves on the enamel surface and predispose teeth to caries. The aim of this study was to compare the two common stripping systems and the effect of polishing after the stripping process on the level of surface roughness. Materials and Methods: Twenty-five extracted teeth were randomly divided into five groups:untreated control (C), mechanical stripping (Sn), mechanical stripping and polishing (Sp), manual stripping (Dn), manual stripping and polishing (Dp). Mechanical stripping used a SWISS denta care Quality oscillating system, manual stripping used Dentaurum hand tapes, and polishing used Soflex disks. Teeth underwent pH-cycling and the amount of released calcium was measured by spectrometry. Samples were examined under atomic force microscopy (AFM) before and after demineralization. Baseline and post-demineralization surface roughness values (Ra, Rq, Rz) were calculated. Finally, scanning electron microscopy (SEM) images were obtained from two samples of each group. data analysis was done by One-way ANOVA. P-value<0.05 was considered statistically significant. Results: There was no significant difference between the stripping methods in terms of surface roughness parameters, but there was a significant difference between the polished and unpolished groups (P<0.05). Calcium release was significantly lower in the Sp group compared to the other groups (P<0.05). In SEM images, the least amount enamel damage was related to the Sp group. Conclusion: Unlike stripping systems, polishing is considerably useful in decreasing enamel roughness. Totally, mechanical stripping and polishing has shown the best consequences on enamel structure.","PeriodicalId":12445,"journal":{"name":"Frontiers in Dentistry","volume":"23 ","pages":"10"},"PeriodicalIF":0.0,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Fresh Orange Juice and Industrial Orange Juice on Enamel Microhardness of Primary Teeth: An In-Vitro Study. 鲜橙汁和工业橙汁对乳牙牙釉质显微硬度影响的体外研究。

Frontiers in Dentistry Pub Date : 2026-02-22 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i9.21275

Parisa Kazemi Roudsari, Hajar Attarzadeh

{"title":"Effect of Fresh Orange Juice and Industrial Orange Juice on Enamel Microhardness of Primary Teeth: An In-Vitro Study.","authors":"Parisa Kazemi Roudsari, Hajar Attarzadeh","doi":"10.18502/fid.v23i9.21275","DOIUrl":"10.18502/fid.v23i9.21275","url":null,"abstract":"Objectives: Increased consumption of non-alcoholic carbonated soft drinks, diet sodas, and fruit juices greatly contributes to dental erosion. This study aimed to compare the microhardness of primary tooth enamel before and after exposure to fresh and industrial (commercial) orange juices. Materials and Methods: In this in vitro experimental study, 30 enamel blocks were obtained from primary teeth, and randomly divided into three groups (n=10). The pH levels of fresh and commercial orange juices were measured. The enamel blocks were immersed in fresh and industrial orange juices for 10 minutes, 4 times a day, over a period of 15 days. Specimens in the third group were stored in artificial saliva to serve as the control group. Enamel microhardness was assessed using the Vickers hardness test both before and after immersion in orange juices. Data were analyzed using paired t-test and ANOVA (alpha=0.05). Results: There was a significant decrease in enamel microhardness in both the fresh and commercial orange juice groups (P<0.001), with no significant difference in this regard between the two orange juice groups (P=0.209). Conclusion: The microhardness of primary enamel can decrease following exposure to both fresh and commercial orange juices, but no significant difference was found between the two types regarding the enamel microhardness reduction.","PeriodicalId":12445,"journal":{"name":"Frontiers in Dentistry","volume":"23 ","pages":"9"},"PeriodicalIF":0.0,"publicationDate":"2026-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Hypericum perforatum on Early Wound Healing after Tooth Extraction: A Randomized Controlled Clinical Trial. 贯叶连翘对拔牙后早期创面愈合的影响：一项随机对照临床试验。

Frontiers in Dentistry Pub Date : 2026-02-20 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i8.21237

Sogand Haghighatpasand, Masoud Moradi, Farshad Moradi, Faranak Shafiee

{"title":"Effect of Hypericum perforatum on Early Wound Healing after Tooth Extraction: A Randomized Controlled Clinical Trial.","authors":"Sogand Haghighatpasand, Masoud Moradi, Farshad Moradi, Faranak Shafiee","doi":"10.18502/fid.v23i8.21237","DOIUrl":"10.18502/fid.v23i8.21237","url":null,"abstract":"Objectives: Hypericum perforatum (H. perforatum) has natural anti-inflammatory properties when used as a dressing on ulcers and burn wounds. The aim of this study was to investigate the effect of topical application of hydroethanolic gel of H. perforatum on soft tissue healing in tooth extraction sockets. Materials and Methods: This split-mouth randomized controlled clinical trial was performed on 30 patients aged 18-30 years who required simultaneous extraction of maxillary premolars bilaterally for orthodontic reasons. After tooth extraction, 2mL of 3% H. perforatum gel was injected into the extraction socket in the intervention side. Pain was measured based on a Numeric Rating Scale (NRS). Socket healing was assessed using the standardized Landry, Turnbull, and Howley index. Repeated measures ANOVA and generalized estimating equations (GEE) were employed to compare different indices between the two groups (alpha=0.05). Results: The mean age of the participants was 23.53 years. Of all, 25 were females and 5 were males. The intervention side had a significantly lower pain than the control side (P<0.05). The wound size decreased at 3 and 7 days, and it was smaller in the intervention side than the control side (P<0.05). The healing index score was significantly higher in the intervention side than the control side (P<0.001). Conclusion: The results showed that 3% H. perforatum hydroethanolic gel was effective for promoting soft tissue healing in tooth extraction sockets, decreasing pain, and improving the wound healing index.","PeriodicalId":12445,"journal":{"name":"Frontiers in Dentistry","volume":"23 ","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2026-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of 660 and 940nm Low-Level Laser Irradiation on Viability and Proliferation of Stem Cells from Human Exfoliated Deciduous Teeth. 660和940nm低强度激光照射对人脱落乳牙干细胞活力和增殖的影响。

Frontiers in Dentistry Pub Date : 2026-02-10 eCollection Date: 2026-01-01 DOI: 10.18502/fid.v23i7.21236

Samar Rahimpour, Arash Azizi, Mahshid Hodjat, Mohammad Vahedi

{"title":"Effects of 660 and 940nm Low-Level Laser Irradiation on Viability and Proliferation of Stem Cells from Human Exfoliated Deciduous Teeth.","authors":"Samar Rahimpour, Arash Azizi, Mahshid Hodjat, Mohammad Vahedi","doi":"10.18502/fid.v23i7.21236","DOIUrl":"10.18502/fid.v23i7.21236","url":null,"abstract":"Objectives: This study evaluated the effects of 660 and 940nm lasers on viability and proliferation of stem cells from human exfoliated deciduous teeth (SHED). Materials and Methods: SHED were cultured and divided into six groups for laser irradiation: 660nm laser at 25mW with energy densities of 1 and 2J/cm2 for 8 and 16 seconds, and 940nm laser at 100mW with the same energy densities and durations. Two additional groups served as controls with no laser exposure. Cell viability was evaluated by the methyl thiazolyl tetrazolium (MTT) assay, and cell proliferation was measured by cell counting with a Neubauer chamber and qualitatively evaluated by 4',6-diamidino-2-phenylindole (DAPI) staining at 24 and 72 hours post- irradiation. Data were analyzed using one-way ANOVA, followed by Tamhane's and Tukey's tests. Results: Irradiation with 940nm laser enhanced cell proliferation compared to both 660nm laser and control groups at 24 and 72 hours (P<0.05), as confirmed by DAPI staining. At 24 hours, all laser-treated groups showed increased cell viability relative to the control, although the increase in the 660nm/2J/cm² group was not statistically significant (P>0.05). At 72 hours, only 940nm laser group showed significantly higher cell viability and proliferation than the control group (P<0.05). Conclusion: These findings suggest low-level laser therapy, particularly at the wavelength of 940nm enhances the viability and proliferation of stem cell, supporting its potential as a promising tool in regenerative medicine and tissue engineering.","PeriodicalId":12445,"journal":{"name":"Frontiers in Dentistry","volume":"23 ","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13428510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148653064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0