Food Quality and Safety最新文献_第9页

Comparative analysis of fruit firmness and genes associated with cell wall metabolisms in three cultivated strawberries during ripening and postharvest 三种栽培草莓成熟期和采后果实硬度及细胞壁代谢相关基因的比较分析

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-04-15 DOI: 10.1093/fqsafe/fyad020

Yi-Bo Ren, Bai-Jun Li, Hao-Ran Jia, Xiaofang Yang, Yunfan Sun, Jia-Han Shou, G. Jiang, Yanna Shi, Kun-song Chen

{"title":"Comparative analysis of fruit firmness and genes associated with cell wall metabolisms in three cultivated strawberries during ripening and postharvest","authors":"Yi-Bo Ren, Bai-Jun Li, Hao-Ran Jia, Xiaofang Yang, Yunfan Sun, Jia-Han Shou, G. Jiang, Yanna Shi, Kun-song Chen","doi":"10.1093/fqsafe/fyad020","DOIUrl":"https://doi.org/10.1093/fqsafe/fyad020","url":null,"abstract":"\u0000 Cultivated strawberry (Fragaria × ananassa), a world-famous fruit, is subjected to rapid softening during ripening, resulting in a shorter shelf life and severe economic losses during storage and transportation. However, there is limited understanding of the molecular mechanism underlying differences in fruit firmness during ripening and postharvest among cultivated strawberries. Here, we explored this molecular mechanism by comparing three cultivated strawberries via firmness measurement, transcriptome analysis, quantitative real-time PCR, and correlation analysis, and revealed that FaEXP7, FaPG2, FaPLA, and Faβ-Gal4, as potential softening activators expressed before harvest to determine fruit with more softened texture and shorter shelf life, and the extremely high expression levels of FaCEL1-1 and FaCEL1-3 during ripening might be accelerators to intensify this situation. Additionally, both the enzyme activities of FaCEL and the expression pattern of FaCEL1-3 showed a significantly negative correlation with fruit firmness after harvest, suggesting that FaCEL1-3 might play a key role in promoting strawberry fruit softening not only during ripening but also postharvest. These results showed that the difference in fruit firmness and shelf life among cultivated strawberries was controlled by the temporal expression pattern of a legion of cell wall-associated genes during ripening and postharvest.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46836693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal-spatial analysis of cyromazine in cowpea using liquid chromatography-mass spectrometry coupled with mass spectrometry imaging 液相色谱-质谱联用-质谱成像技术分析豇豆中氯丙嗪的时空分布

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-04-14 DOI: 10.1093/fqsafe/fyad021

Xue-Jiao Tang, Congling Huang, Jianxiang Huang, Jiale Wang, Zhiting Chen, Yan Chen, Zemiao Xiao, K. Wan

{"title":"Temporal-spatial analysis of cyromazine in cowpea using liquid chromatography-mass spectrometry coupled with mass spectrometry imaging","authors":"Xue-Jiao Tang, Congling Huang, Jianxiang Huang, Jiale Wang, Zhiting Chen, Yan Chen, Zemiao Xiao, K. Wan","doi":"10.1093/fqsafe/fyad021","DOIUrl":"https://doi.org/10.1093/fqsafe/fyad021","url":null,"abstract":"\u0000 \u0000 \u0000 Cowpea (Vigna unguiculata L. Walp.) is an economically important crop. It is nutritious and popular with consumers. However, it has been listed as one of the agricultural products of critical concern about safety in China and cyromazine is the major risk factor.\u0000 \u0000 \u0000 \u0000 This study analyzed the dissipation and permeation kinetics of cyromazine residue in cowpea, to offer a scientific basis for the rational use of pesticide and ensuring the safety of agricultural products.\u0000 \u0000 \u0000 \u0000 Investigated the dissipation and residue level of the systemic insecticide cyromazine on cowpea under field and stored conditions. Subsequently, studied the spatial distribution of cyromazine using MSI to visualize the dynamic processes of permeation and migration in the tissues post pesticide application.\u0000 \u0000 \u0000 \u0000 The dissipation processing of cyromazine in cowpea was shown to follow the first-order kinetics and half-life was 7.76 days in the field. In cowpea, the permeation and migration rate of cyromazine was quite faster than that in the kidney beans and accumulation mainly in the pulp. It is not safe to apply cyromazine to cowpeas with reference to the application method on kidney beans.\u0000 \u0000 \u0000 \u0000 These findings present vital data for the determination of risks linked with cowpea consumption and pesticide intake.\u0000","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48151112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of HMW-GS Dx2 absence on the protein aggregation characteristics and thermal stability of wheat flour during maturation HMW-GS Dx2缺失对小麦粉成熟过程中蛋白质聚集特性及热稳定性的影响

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-03-23 DOI: 10.1093/fqsafe/fyad019

Liye Zhao, Lijun Song, Liqun Li, Xuejun Li

{"title":"Effects of HMW-GS Dx2 absence on the protein aggregation characteristics and thermal stability of wheat flour during maturation","authors":"Liye Zhao, Lijun Song, Liqun Li, Xuejun Li","doi":"10.1093/fqsafe/fyad019","DOIUrl":"https://doi.org/10.1093/fqsafe/fyad019","url":null,"abstract":"\u0000 \u0000 \u0000 Wheat flour maturation affects the aggregation and structural stability of proteins. The number of high-molecular-weight glutenin subunits (HMW-GSs) differs in various wheat varieties. The effects of Dx2 absence on the protein aggregation characteristics and thermal stability of flour were investigated during 120 days of maturation using near-isogenic lines (NILs).\u0000 \u0000 \u0000 \u0000 The absence of Dx2 delayed and decreased the protein aggregation of flours during the maturation, i.e., the maturation-induced increases were later and smaller for glutenin, glutenin macropolymer (GMP), glutenin/gliadin ratio, 𝛽-sheets, and 𝛽-sheet/𝛼-helix ratio in HMW-D1a without Dx2 than those in HMW-D1p with Dx2; these differences were ascribed to the weaker interactions between the sulfhydryl (-SH) groups, disulfide bonds (-S-S-), and hydrophobicity in the flours without Dx2. Flour maturation caused the dough microstructures to be more compact and denser, thereby increasing the flour thermal stability as observed by a higher denaturation peak temperature (Tp), enthalpy of thermal transition (ΔH), and degradation temperature (Td), These changes led to better dough properties such as dough development time, dough stability time, and protein weakening, but the optimal stage in HMW-D1a without Dx2 was reached later.\u0000 \u0000 \u0000 \u0000 These findings deepen the understanding of how HMW-GS Dx2 modifies protein structures during flour maturation.\u0000","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43354766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The difference in composition and nutritional potency of honey extracted by centrifugation and pressed processes 离心法和压榨法提取蜂蜜的成分和营养功效差异

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-03-22 DOI: 10.1093/fqsafe/fyad018

Yueyang Hu, Zhen Li, Shoucheng Wang, Xiao-bo Wu

引用次数: 0

Effect of different forms of selenium in osteoporosis rat model induced by retinoic acid 不同形式硒对维甲酸所致骨质疏松大鼠模型的影响

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-03-09 DOI: 10.1093/fqsafe/fyad017

Haitao Li, Yan Guo, Xiaoyan Chen, Zhengyin Man, Xiaotao Zhang

{"title":"Effect of different forms of selenium in osteoporosis rat model induced by retinoic acid","authors":"Haitao Li, Yan Guo, Xiaoyan Chen, Zhengyin Man, Xiaotao Zhang","doi":"10.1093/fqsafe/fyad017","DOIUrl":"https://doi.org/10.1093/fqsafe/fyad017","url":null,"abstract":"\u0000 Osteoporosis is a typical physiological disease, and the main symptoms of which are brittle fracture, bone pain and the easily deformed spine. As the aging is increasing, the prevalence of osteoporosis increases year by year. In the present study, selenium with antioxidant, immunomodulatory and anti-tumor effects was used to prevent osteoporosis induced by retinoic acid. The serum calcium contents in the selenium-treated groups (sodium selenite and selenomethionine) were significantly higher (P<0.05) than that in the model group both in prevention and treatment studies. After prevention, glutamate transaminase (GOT), glutamate transaminase (GPT), alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRACP) levels were significantly (P<0.05) decreased. In the treatment study, the serum calcium and phosphorus contents of the rats increased after selenium treatment. There was no significant change (P>0.05) in the activity of GOT and GPT. The content of ALP decreased obviously and the TRACP enzyme activity was increased. Overall, these results showed that different forms of selenium compounds have great potentials in preventing and treating osteoporosis.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2023-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46038528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Universal probe with oriented antibody to improve the immunochromatographic assay of lead ions in Procambarus clarkii 定向抗体通用探针改进克氏原螯虾中铅离子的免疫层析分析

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-02-27 DOI: 10.1093/fqsafe/fyad015

Yangyang Li, Zhengwei Zhu, Wenli Qu, Qing Yang, Yan Liu, Qiaotong Wang, Shuo Duan, Jin Wu, Zhiyong Gong, Lin Xu

{"title":"Universal probe with oriented antibody to improve the immunochromatographic assay of lead ions in Procambarus clarkii","authors":"Yangyang Li, Zhengwei Zhu, Wenli Qu, Qing Yang, Yan Liu, Qiaotong Wang, Shuo Duan, Jin Wu, Zhiyong Gong, Lin Xu","doi":"10.1093/fqsafe/fyad015","DOIUrl":"https://doi.org/10.1093/fqsafe/fyad015","url":null,"abstract":"\u0000 \u0000 \u0000 Based on the information from the random inspection of foods by the China Food and Drug Administration in 2022, the contamination levels of lead ions are high in many edible products. Traditional methods of detecting lead ions cannot meet the requirements of on-site analysis of food due to the need for large equipment. The immunochromatographic assay (ICA) is an effective, rapid, on-site analytical technique for determining lead ions in foods. However, the performance of ICA based on the traditional probe (AuNP-mAb) is limited by ignoring the influence of the antibody orientation.\u0000 \u0000 \u0000 \u0000 In this study, we developed an efficient technology for constructing a universal probe (AuNP-PrA-mAb) based on oriented immobilization of antibody. The performance of ICA was largely improved due to specific binding of the Fc region of the antibody with recombinant protein A (PrA) on the surface of a gold nanoparticle (AuNP). The ICA based on a universal probe was applied for the qualitative and quantitative detection of lead ions in Procambarus clarkii within 30 min. Meanwhile, a simple and fast pretreatment method based on dilute acid extraction was developed for pretreating the Procambarus clarkii containing lead ions.\u0000 \u0000 \u0000 \u0000 The visual limit of detection (vLOD) and the scanning limit of quantization (sLOQ) of the developed ICA strip for lead ions were 0.5 ng/mL and 0.28 ng/mL, respectively. The sensitivity of ICA based on universal probe was 10-fold higher than that of the ICA using traditional probe. Furthermore, the detection results had no obvious difference between the ICA and ICP-MS with T-test statistical method.\u0000 \u0000 \u0000 \u0000 The developed ICA that is based on a universal probe presented broad application prospects in detecting contaminants in foods.\u0000","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46739638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Malondialdehyde treatment reduced immunoreactivity of amandin and delayed its digestion 丙二醛处理降低了芒果素的免疫反应性，延缓了其消化

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-02-23 DOI: 10.1093/fqsafe/fyad013

Xin Luo, Mingyan Ai, Yongyan Wu, Qia Wang, Hongbo Song, Qun Huang, Jiankang Lu

{"title":"Malondialdehyde treatment reduced immunoreactivity of amandin and delayed its digestion","authors":"Xin Luo, Mingyan Ai, Yongyan Wu, Qia Wang, Hongbo Song, Qun Huang, Jiankang Lu","doi":"10.1093/fqsafe/fyad013","DOIUrl":"https://doi.org/10.1093/fqsafe/fyad013","url":null,"abstract":"\u0000 \u0000 \u0000 The secondary products of lipid oxidation are one of the main factors inducing protein oxidation. The effects of oxidation treatment with malondialdehyde (MDA) on immunoreactivity of amandin and its digestion were studied.\u0000 \u0000 \u0000 \u0000 The rabbit IgG binding ability of amandin was analyzed by western blotting, and the changes of oxidation on amandin and the immunoreactivity during digestion of amandin with different degrees of oxidation were investigated in combination with an almond allergen ELISA kit. Alteration of linear epitopes of amandin by oxidation was investigated by LC-MS/MS.\u0000 \u0000 \u0000 \u0000 The results showed that immunoreactivity of amandin was significantly reduced after 1 and 100 mmol/L MDA treatment. However, the 1 mmol/L MDA treatment was owing to cleavage of linear epitope peptide in amandin and oxidation of the active amino acid. The 100 mmol/L MDA treatment was due to aggregation of amandin and significant decrease in its solubility. Oxidation also reduced digestibility of amandin and significantly affected immunoreactivity during digestion. LC-MS/MS also identified four oxidation-prone methionine sites (aa 264-274, 298-308, 220-240 and 275-297) in Gamma conglutinin 1.\u0000 \u0000 \u0000 \u0000 MDA treatment reduced the immunoreactivity of amandin. MDA treatment also led to protein aggregation, which slowed down the digestion of amandin and altered immunoreactivity of amandin during digestion.\u0000","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45818319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of a novel Bacillus methylotrophicus phage BM-P1 新型甲基营养芽孢杆菌噬菌体BM-P1的特性研究

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-02-22 DOI: 10.1093/fqsafe/fyad016

Ruirui Lv, Mingjie Xu, Shengye Guo, Jingwei Yao, H. A. Sakandar, Jing Guo, Can Zhang, Xia Chen

{"title":"Characterization of a novel Bacillus methylotrophicus phage BM-P1","authors":"Ruirui Lv, Mingjie Xu, Shengye Guo, Jingwei Yao, H. A. Sakandar, Jing Guo, Can Zhang, Xia Chen","doi":"10.1093/fqsafe/fyad016","DOIUrl":"https://doi.org/10.1093/fqsafe/fyad016","url":null,"abstract":"\u0000 Bacillus species have been widely used as probiotics in a number of food products. However, these are vulnerable to bacteriophage infections which poses fermentation failure and eventually result in economic losses. Given this, we designed this study in which bacteriophage of lysogenic Bacillus methylotrophicus, Phage BM-P, was induced successfully, and its biological characteristics and genome information were researched. The obtained results showed that Bacillus species phage belonged to Myoviridae family. The maximum number of infections (MOI) for this phage was 10, with a burst size of 104.48 ± 2.70 counts expressed per milliliter per infective center. Additionally, this phage was temperature and pH sensitive and divalent ions (Ca 2+, Mg 2+) and chloramphenicol did not express significant influence on its adsorption capacity. Genomic analysis revealed that the genome size of Phage BM-P1 was 153,087 bp having 41.94% GC content, including 258 coding sequences (CDs). Further, 52 bacteriophages of Bacillus were classified into two clades by using phylogenetic analysis. Among them, phage BM-P1 with phage VB_BsuM-Goe3 and vB_BveM-Goe7 had highest ANI values, 95.23% and 95.28%, respectively. However, synteny analysis unraveled transpose, deletion and insertion in the genome of BM-P1. Considering this, it could be extrapolated that this phage is a new lysogenic phage. In conclusion, this study furthers the knowledge on the properties of Bacillus methylotrophicus phages and provides seminal insights for designing effective antiphage strategies for fermentation industry.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42409133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Epsilon-poly-L-lysine increases disease resistance of citrus against postharvest green mold by activating amino acid metabolism and phenolic compounds biosynthesis Epsilon-poly-L-赖氨酸通过激活氨基酸代谢和酚类化合物生物合成提高柑橘对采后绿霉菌的抗病性

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-02-08 DOI: 10.1093/fqsafe/fyad010

Xiong Zhang, Qian Deng, Wenjun Wang, Hongyan Zhang, Ou Chen, K. Zeng

{"title":"Epsilon-poly-L-lysine increases disease resistance of citrus against postharvest green mold by activating amino acid metabolism and phenolic compounds biosynthesis","authors":"Xiong Zhang, Qian Deng, Wenjun Wang, Hongyan Zhang, Ou Chen, K. Zeng","doi":"10.1093/fqsafe/fyad010","DOIUrl":"https://doi.org/10.1093/fqsafe/fyad010","url":null,"abstract":"\u0000 As a famous fruit worldwide, citrus is susceptible to green mold caused by Penicillium digitatum, which causes large economic losses every year. ε-Poly-L-Lysine (ε-PL) is a novel preservative with strong inhibitory effects on fungi, and has the capacity to induce disease resistance in fruit, but the mechanism was rarely reported, especially in citrus. The present study, 800 μg mL -1 ε-PL and P. digitatum spores were inoculated in two different wounds on the citrus pericarp interval of 24 h. The results revealed that ε-PL inhibited the development of green mold without direct contact with P. digitatum, indicating the disease resistance of citrus was activated. Transcriptome analysis revealed that ε-PL activated amino acid metabolism and phenylpropanoid biosynthesis. Besides, the accumulation of glutamic acid, proline, arginine, serine, lysine, phenylalanine, and tyrosine were changed during storage. In the phenylpropanoid biosynthesis, ε-PL increased PAL, C4H, and 4CL activities and total phenolic and flavonoid contents. Importantly, among these phenolic compounds, ε-PL promoted the accumulation of individual phenolic compounds including ferulic acid, chlorogenic acid, p-coumaric acid, caffeic acid, gallic acid, catechins, epicatechin, and narirutin. In conclusion, ε-PL enhanced resistance of citrus through amino acid metabolism and phenolic compounds accumulation. These results improved the knowledge on the mechanism of ε-PL induced disease resistance, and also provided a fresh theoretical basis for the use of ε-PL in post-harvest citrus preservation.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2023-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42972237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A rapid method on identifying mastitis degrees of bovines based on dielectric spectra of raw milk 基于生乳介电光谱的奶牛乳腺炎程度快速鉴定方法

IF 5.6 3区农林科学

Food Quality and Safety Pub Date : 2023-02-07 DOI: 10.1093/fqsafe/fyad014

Zhuozhuo Zhu, Biying Lin, Xinhua Zhu, Wenchuan Guo

{"title":"A rapid method on identifying mastitis degrees of bovines based on dielectric spectra of raw milk","authors":"Zhuozhuo Zhu, Biying Lin, Xinhua Zhu, Wenchuan Guo","doi":"10.1093/fqsafe/fyad014","DOIUrl":"https://doi.org/10.1093/fqsafe/fyad014","url":null,"abstract":"\u0000 Bovine mastitis is the most complex and costly disease in the worldwide dairy industry. Somatic cell count (SCC) is accepted as an international standard for diagnosing the mastitis of cows, but most instruments used to detect SCC are expensive, or the detection speed is very low. To develop a rapid method for identifying mastitis degree, the dielectric spectra of 301 raw milk samples at three mastitis grades, i.e., negative, weakly positive, and positive grades based on SCC, were obtained in the frequency range of 20-4500 MHz using coaxial probe technology. Variable importance in the projection method was used to select characteristic variables, and principal component analysis (PCA) and partial least squares (PLS) were used to reduce data dimension. The linear discriminant analysis, support vector classification (SVC), and feed-forward neural network models were established to predict mastitis degrees of cows based on 22 principal components and 24 latent variables obtained by PCA and PLS, respectively. The results showed that the SVC model with PCA had the best classification performance with an accuracy rate of 95.8% for the prediction set. The research indicates that dielectric spectroscopy technology has a great potential in developing a rapid detector to diagnose mastitis of cows in-situ or online.","PeriodicalId":12427,"journal":{"name":"Food Quality and Safety","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41786817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1